Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,057,798 C→A 5.5% R165L (CGA→CTA)  USA300HOU_1913 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,057,7980CA 5.5% 262.5 / 2.4 71R165L (CGA→CTA) USA300HOU_1913hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (41/26);  new base A (2/2);  total (43/28)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCGTGACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACGCAGCATAGTGCGT  >  CP000730/2057658‑2057934
                                                                                                                                            |                                                                                                                                        
tCGTGACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTc                                                                                                                                          <  1:316267/141‑1 (MQ=255)
tCGTGACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTc                                                                                                                                          <  2:298312/141‑1 (MQ=255)
tCGTAACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTc                                                                                                                                          <  2:39284/141‑1 (MQ=255)
 cGTGACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCg                                                                                                                                         >  1:339988/1‑141 (MQ=255)
 cGTGACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCg                                                                                                                                         >  1:171690/1‑141 (MQ=255)
   tGACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAg                                                                                                                                       >  1:224996/1‑141 (MQ=255)
      caaacaaTCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGtt                                                                                                                                    >  1:99033/1‑141 (MQ=255)
      caaacaaTCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTGAGTCCAGAATCCACCATGTATGTTTCGAGGtt                                                                                                                                    >  1:331532/1‑141 (MQ=255)
       aaacaaTCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTc                                                                                                                                   >  1:79341/1‑141 (MQ=255)
       aaacaaTCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTc                                                                                                                                   >  1:24035/1‑141 (MQ=255)
       aaacaaTCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTc                                                                                                                                   >  2:302353/1‑141 (MQ=255)
         acaaTCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCAt                                                                                                                                 <  2:147843/141‑1 (MQ=255)
          caaTCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATa                                                                                                                                >  2:319189/1‑141 (MQ=255)
              cAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCa                                                                                                                            <  2:139142/141‑1 (MQ=255)
                 cAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATg                                                                                                                         >  1:203139/1‑141 (MQ=255)
                 cAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATg                                                                                                                         >  2:64571/1‑141 (MQ=255)
                 cAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATg                                                                                                                         >  2:293094/1‑141 (MQ=255)
                                  tttACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATc                                                                                                        <  2:374193/141‑1 (MQ=255)
                                        ttGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAAtt                                                                                                  <  2:313587/141‑1 (MQ=255)
                                        ttGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAAtt                                                                                                  <  2:387281/141‑1 (MQ=255)
                                                  tGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTa                                                                                        >  1:132939/1‑141 (MQ=255)
                                                           ccacTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCCGAGCTATATTGTTTATCGTATCCATCAAt                                                                               <  2:116381/140‑1 (MQ=255)
                                                           acacTTTCCTGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTTATACGCAATGATAAATGATTTAGGATATAAGTGTTATATCGTATcctttttt                                                                               <  2:213473/141‑7 (MQ=255)
                                                           acacTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAAt                                                                               <  2:427583/141‑1 (MQ=255)
                                                            cacTTTCCAGTTCTTCTGGTTCATCATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAAtt                                                                              <  2:400219/141‑1 (MQ=255)
                                                            cacTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAAtt                                                                              <  2:82663/141‑1 (MQ=255)
                                                            cacTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAAtt                                                                              <  2:146587/141‑1 (MQ=255)
                                                                  ccAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGtt                                                                        >  1:165153/1‑141 (MQ=255)
                                                                  ccAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGtt                                                                        >  1:53206/1‑141 (MQ=255)
                                                                   cAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTc                                                                       >  1:165239/1‑141 (MQ=255)
                                                                           ctGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTc                                                               >  2:139259/1‑141 (MQ=255)
                                                                           ctGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTc                                                               >  2:166485/1‑141 (MQ=255)
                                                                                tCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCGCCATGTATGTTTAGAGGTTCCTCGGCAGTGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgt                                                          <  1:389305/141‑1 (MQ=255)
                                                                                tCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgt                                                          <  1:236366/141‑1 (MQ=255)
                                                                                tCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgt                                                          <  1:43115/141‑1 (MQ=255)
                                                                                tCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTAGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgt                                                          <  1:212062/141‑1 (MQ=255)
                                                                                 cATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgtg                                                         <  1:319189/141‑1 (MQ=255)
                                                                                  aTAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTTTCGTTTTCTTCAATTTACGTTCATATTTTCTTGgtgt                                                        >  2:349179/1‑141 (MQ=255)
                                                                                  aTAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgtgt                                                        <  1:211204/141‑1 (MQ=255)
                                                                                  aTAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgtgt                                                        >  2:2012/1‑141 (MQ=255)
                                                                                  aTAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTAGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgtgt                                                        >  2:410581/1‑141 (MQ=255)
                                                                                  aTAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTAGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGgtgt                                                        >  2:410527/1‑141 (MQ=255)
                                                                                     aTCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGCATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGCTCTAAATTTTTTATCGTCTCCAGCAATTTACGTTCATATTTTCTTGGTGTTaa                                                     >  1:331614/1‑141 (MQ=255)
                                                                                      tCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTaaa                                                    >  2:309412/1‑141 (MQ=255)
                                                                                         ttAAGCTTTCTCCGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAAtt                                                 <  2:375040/141‑1 (MQ=255)
                                                                                         ttAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAAtt                                                 <  2:188522/141‑1 (MQ=255)
                                                                                          tAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAAttt                                                <  1:188424/141‑1 (MQ=255)
                                                                                          tAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAAttt                                                <  2:142978/141‑1 (MQ=255)
                                                                                          tAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAAttt                                                <  2:74439/141‑1 (MQ=255)
                                                                                             gCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCa                                             >  2:278592/1‑141 (MQ=255)
                                                                                             gCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCa                                             >  2:237992/1‑141 (MQ=255)
                                                                                             gCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCa                                             >  2:347499/1‑141 (MQ=255)
                                                                                             gCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCa                                             >  2:138589/1‑141 (MQ=255)
                                                                                                    acgacgAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCAt                                      >  2:238864/1‑141 (MQ=255)
                                                                                                        cgaATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGa                                  >  1:181620/1‑141 (MQ=255)
                                                                                                         gaATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGAc                                 <  1:414621/141‑1 (MQ=255)
                                                                                                                tttAGTCCAGAATCCATCATGTATGTTTCTATTTTCATACGCAATGATAAATGCTTTCTCATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCAtc                          <  2:143271/141‑1 (MQ=255)
                                                                                                                  tAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGCTAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCAtctc                        >  1:195114/1‑141 (MQ=255)
                                                                                                                  tAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCAtctc                        >  1:100046/1‑141 (MQ=255)
                                                                                                                    gTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTa                      <  2:285674/141‑1 (MQ=255)
                                                                                                                     tCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTAc                     <  2:224996/141‑1 (MQ=255)
                                                                                                                      ccAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTAcc                    <  2:132939/141‑1 (MQ=255)
                                                                                                                         gAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCaaa                 >  1:287468/1‑141 (MQ=255)
                                                                                                                         gAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCaaa                 >  1:95363/1‑141 (MQ=255)
                                                                                                                         gAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCaaa                 >  1:247810/1‑141 (MQ=255)
                                                                                                                            tCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACgc              >  2:333817/1‑141 (MQ=255)
                                                                                                                             ccaccaTGTATGTTTCGAGGTTCATACGCAATGATAACTGCTTTCGGATCTAACTTTTTTATTGTCTCTATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATATGCATAAAATATCTAACAAAAgca             >  2:113854/1‑141 (MQ=255)
                                                                                                                              caccaTGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACgcag            >  1:443247/1‑141 (MQ=255)
                                                                                                                              caccaTGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACgcag            >  1:65552/1‑141 (MQ=255)
                                                                                                                                ccaTGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACgcagca          >  1:115837/1‑141 (MQ=255)
                                                                                                                                       tgtTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACGCAGCATAGTGCg   <  1:340775/141‑1 (MQ=255)
                                                                                                                                        gtTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAGCGCAGCATAGTGCGt  >  1:381687/1‑141 (MQ=255)
                                                                                                                                        gtTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACGCAGCATAGTGCGt  >  2:451430/1‑141 (MQ=255)
                                                                                                                                        gtTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTATACCAAACGCAGCATAGTGCGt  >  2:42135/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TCGTGACAAACAATCAGCAATTGTTTCATTCTCATTTACTTTGAATTTCATGTTCTACTACACTTTCCAGTTCTTCTGGTTCATAATCTTTAAGCTTTCTACGACGAATGCCTTTAGTCCAGAATCCACCATGTATGTTTCGAGGTTCATACGCAATGATAAATGCTTTCGGATCTAAATTTTTTATCGTATCCATCAATTTACGTTCATATTTTCTTGGTGTTAAAATTTGCATCACCATACGACTACCATCTCTACCAAACGCAGCATAGTGCGT  >  CP000730/2057658‑2057934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: