BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,015	C→T	27.2%	R179R (AGG→AGA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,015	0	C	T	27.2%	824.4 / inf	559	R179R (AGG→AGA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (218/180); new base T (81/72); total (307/252)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 575 total aligned reads displayed.

TTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAG‑GTGCCTGAT  >  CP000730/35877‑36154

ttAACTTGCTAGCATGATGCTAATTTCTCGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                                               <  1:547860/140‑1 (MQ=255)

  aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                                             <  2:545751/140‑1 (MQ=33)

   aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTTGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGGGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                                            <  1:253922/140‑1 (MQ=16)

         tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                      >  2:88740/1‑140 (MQ=17)

         tAGCATGATGCTAATATCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                      >  1:494992/1‑140 (MQ=12)

          aGCCTGATCGTATTTCCGTGGCATGGCTAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATATAGAGCGTCAATACCCTCAATACCTTTTAAAGTATTCTTTGCTGTATTTATACTTTGATACCTTGtctttct                                                                                                                                     <  2:388916/140‑1 (MQ=11)

           gCATGATGCTAATTTCGTGGCATGGCGACAATCAGGCGCTCTGAAGAGACCTGCTGTGCTTTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTTACTTTGATACCTTGtctttctt                                                                                                                                    >  2:275006/1‑140 (MQ=11)

           gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                                                                    >  1:29451/1‑140 (MQ=18)

                attgtAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTTCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                                               <  2:467755/136‑1 (MQ=11)

                atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                                               >  1:558252/1‑140 (MQ=35)

                    tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                                                                                                           >  1:336157/1‑140 (MQ=255)

                     aaTTTGGTGGAATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTTTCTTTCTAGATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCatat                                                                                                                          <  1:454309/140‑1 (MQ=11)

                      aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATAACTTGTCTTTCTTACTTTAATATg                                                                                                                         >  2:57899/1‑140 (MQ=255)

                           gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAACTACAATCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTATCTTTCTTCCTTTAATATGACgat                                                                                                                    >  1:282007/1‑138 (MQ=11)

                            tGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACGTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                   <  1:608332/140‑1 (MQ=255)

                            tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATGTGACGGTg                                                                                                                   >  2:17632/1‑140 (MQ=255)

                            tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                   >  2:44750/1‑140 (MQ=37)

                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATCTAACGGTGa                                                                                                                  >  2:109051/1‑140 (MQ=11)

                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                                                  <  2:563362/140‑1 (MQ=255)

                                  ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTg                                                                                                            <  1:102097/140‑1 (MQ=255)

                                  ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTg                                                                                                            <  1:134736/140‑1 (MQ=255)

                                   gggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGc                                                                                                           <  2:348514/138‑1 (MQ=255)

                                   gCGAAAATCCGTAGATCTGAAGAGACCTGTGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGc                                                                                                           <  1:392957/140‑1 (MQ=255)

                                    ggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAGGGTGAT‑CTTGCt                                                                                                          >  2:480440/2‑140 (MQ=11)

                                    cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGTT‑CTTGCt                                                                                                          >  1:323557/1‑140 (MQ=255)

                                    cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCt                                                                                                          >  2:24202/1‑140 (MQ=255)

                                       aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCaa                                                                                                       <  2:423481/140‑1 (MQ=255)

                                          tCCGTAGATCTTAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGa                                                                                                    <  1:663396/140‑1 (MQ=255)

                                          tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGa                                                                                                    <  1:536485/140‑1 (MQ=38)

                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAATTTAATATGAAGGTGGT‑CTTGCTCAATGAgg                                                                                                  >  2:196154/1‑140 (MQ=255)

                                              tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTGGATACCTTGTCTTTCTTACTTTAATATGACGCTGAT‑CTTGCTCAATGAGGtt                                                                                                >  1:382461/1‑140 (MQ=255)

                                              tAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTGATATGACGGTGAT‑CTTGCTCAATGAGGtt                                                                                                <  2:610087/140‑1 (MQ=11)

                                               aGATCTGAAGAGACCTGAGGTTGTTGGTTTATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGT‑CTTGCTTGATGAGGTTa                                                                                               >  2:642569/1‑140 (MQ=11)

                                                   cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCa                                                                                           >  2:25979/1‑140 (MQ=255)

                                                    tgatgaGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATG‑CCTTGTTGCTTGCTCAATGAGGTTATTCAg                                                                                          <  1:659629/140‑1 (MQ=11)

                                                    tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAg                                                                                          >  2:399241/1‑140 (MQ=255)

                                                    tGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAg                                                                                          <  1:588759/140‑1 (MQ=255)

                                                     gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGa                                                                                         <  1:203122/140‑1 (MQ=255)

                                                         agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATAtt                                                                                     <  2:122629/140‑1 (MQ=255)

                                                            ccTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGTGGGTATTCAGATATTTCg                                                                                  >  2:62862/1‑140 (MQ=11)

                                                             ctacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTAAGATATTTCGa                                                                                 >  1:117734/4‑140 (MQ=255)

                                                             cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGa                                                                                 >  2:343823/1‑140 (MQ=255)

                                                                cGGTTCTTTTTATCAGGGTCGTAAATACCCTCAATACCTTTTATAGTATTCTTTGCTGTCTTGTGACTTTGATACCTTGACTGTCTTTCTATAATATGACGGTGAT‑CTTGCTCAATGTGGTGATTCAGATATTTctctat                                                                              <  2:281853/140‑6 (MQ=255)

                                                                cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                              <  1:165207/140‑1 (MQ=38)

                                                                         ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTAGATGTACAATGACa                                                                     >  1:106470/1‑140 (MQ=255)

                                                                          tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                    <  2:264490/140‑1 (MQ=255)

                                                                           atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                   >  1:629438/1‑140 (MQ=255)

                                                                              taGGGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑g                                                               <  2:116986/140‑1 (MQ=255)

                                                                               agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑gg                                                              <  1:441791/140‑1 (MQ=255)

                                                                               agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGACATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑gg                                                              <  2:438705/140‑1 (MQ=255)

                                                                                gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATAGCAGTCA‑Ggt                                                             >  1:318550/1‑140 (MQ=255)

                                                                                  gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑Ggttt                                                           <  1:646304/140‑1 (MQ=255)

                                                                                    gTAAATACACTCAATACCTTTTAAAGTAATCTTTACTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaa                                                         >  1:429026/1‑140 (MQ=14)

                                                                                     tAAATTCACTCAATACCTTTTAGATTAGTCTTTGTTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaag                                                        <  2:360658/140‑1 (MQ=14)

                                                                                     tAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTCTTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑Ggtttaag                                                        >  1:248633/1‑140 (MQ=255)

                                                                                     tAAATACACTCAATACCTTTTAAAGTTTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaag                                                        <  2:616684/140‑1 (MQ=255)

                                                                                      aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaagt                                                       >  1:473959/1‑140 (MQ=255)

                                                                                       aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaagtt                                                      >  2:622599/1‑140 (MQ=255)

                                                                                       aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaagtt                                                      >  1:400025/1‑140 (MQ=255)

                                                                                        aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑Ggtttaagttt                                                     >  2:75918/1‑140 (MQ=255)

                                                                                        aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGCTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaagttt                                                     >  1:448303/1‑140 (MQ=255)

                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑Ggtttaagttta                                                    >  1:62313/1‑140 (MQ=40)

                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCCGTGTTGATACTTTCGTATATTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑Ggtttaagttta                                                    >  1:157335/1‑140 (MQ=14)

                                                                                         tACATTCAATACCTTTTAAACTATTCTTTGCTGTATTGATACTTTGATACCTCGTCTTGCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGCACAATGACAGTCA‑Ggtttaagttta                                                    >  1:406504/1‑140 (MQ=255)

                                                                                          acactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑Ggtttaagtttaa                                                   <  1:568224/136‑1 (MQ=255)

                                                                                           cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTaaa                                                  >  1:26686/4‑140 (MQ=255)

                                                                                            actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTaaaa                                                 >  2:205792/3‑140 (MQ=255)

                                                                                            actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTaaaa                                                 >  2:595728/3‑140 (MQ=255)

                                                                                             ttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTAAGATATGTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAg                                                <  1:188841/140‑1 (MQ=255)

                                                                                              ccAATACCTTTTAAAGTATTCTTAGCAGTCTTGATATTATGATAGCCTGTATTTACTCCTTTAATGTGAAGGTGAT‑CTAGCTCATACTGGTTATTCATATATTTCAATTTACAATG‑CAAACACGGTTTAAGTTTAAAAgt                                               >  2:296844/2‑139 (MQ=2)

                                                                                               cAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTATTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCt                                              >  1:145644/1‑140 (MQ=255)

                                                                                                  tatcTTTTAAAGTATTCTTTGCTGGATTGATACTTTTATTCCTTGTCGTTCTTACTTTAATATGACGGTGAT‑CTTGTTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTa                                           <  2:302571/137‑1 (MQ=14)

                                                                                                   aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGCTATTTCGACGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTaa                                          <  2:333115/140‑1 (MQ=255)

                                                                                                   aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTaa                                          >  1:12067/1‑140 (MQ=255)

                                                                                                    ccTTTTAAAGTATCCTTTGCCGTATCGATACTTTGATCTCTTCTCTTTCTTACTTCAATATGACGGTGAT‑CTTGCTTAATGACGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAAt                                         <  2:37029/140‑1 (MQ=14)

                                                                                                     cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAAtt                                        <  2:508258/140‑1 (MQ=255)

                                                                                                      ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTa                                       >  2:213982/1‑140 (MQ=255)

                                                                                                      ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTa                                       >  2:410901/1‑140 (MQ=40)

                                                                                                            aGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTaa                                 >  1:238360/1‑139 (MQ=255)

                                                                                                               aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCa                              <  2:663097/140‑1 (MQ=255)

                                                                                                                   tttgcgctattctcaCTGTGTTACCTTGTCTGTCTTTCTTTAATAGTACGGTGAT‑CTTTCTCGATGGGGTTATTTACATATTTCTATGTACACTGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAAGCATTGc                          <  2:165345/126‑1 (MQ=2)

                                                                                                                   tttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTATTTTAGCACTTGc                          >  1:81815/1‑140 (MQ=14)

                                                                                                                    ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGGTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCt                         >  2:230248/1‑140 (MQ=255)

                                                                                                                     tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa                        >  1:369070/1‑140 (MQ=255)

                                                                                                                     tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTa                        >  2:156886/1‑140 (MQ=255)

                                                                                                                       cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTGTAACCATTGCTAcc                      >  1:582384/1‑140 (MQ=255)

                                                                                                                         gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGACGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCta                    >  1:304001/1‑139 (MQ=255)

                                                                                                                           aTTGATACTTCGATACCTTGTCTTTCTTACTTTAATATGACGTTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCg                  <  2:518698/140‑1 (MQ=255)

                                                                                                                            ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                 >  1:504411/1‑140 (MQ=255)

                                                                                                                            ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                 >  1:599234/1‑140 (MQ=255)

                                                                                                                            ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                 >  1:530130/1‑140 (MQ=255)

                                                                                                                               aTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAGCATCGATGa              >  2:135870/1‑140 (MQ=14)

                                                                                                                                 aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGTTTATTCATATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTTATAGCTTTAATTACTTTAATCATTGCTACCTTCGTTGagg            >  1:98797/1‑138 (MQ=14)

                                                                                                                                  cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg‑g          <  2:323551/140‑1 (MQ=255)

                                                                                                                                   ctgtaTACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAG‑Gt         <  2:494992/136‑1 (MQ=255)

                                                                                                                                     tGATATCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAG‑GTGc       <  1:416145/140‑1 (MQ=255)

                                                                                                                                     tGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAG‑GTGc       <  1:523047/140‑1 (MQ=255)

                                                                                                                                       atatCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAG‑GTGCCt     <  2:529563/140‑1 (MQ=255)

                                                                                                                                         aCCTTGTCTTTCTTACTTTAATCTGACGGTGAT‑CTTGCTCAATGAGGTTATTCAAATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTT‑GGTGAAGAGTGCCTGa   >  1:215886/1‑140 (MQ=11)

                                                                                                                                          ccTTGTCTTTCTTAGTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAG‑GTGCCTGAt  >  1:364441/1‑140 (MQ=255)

TTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAT‑CTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCA‑GGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAG‑GTGCCTGAT  >  CP000730/35877‑36154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`