BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,174	T→C	26.7%	K126K (AAA→AAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,174	0	T	C	26.7%	482.6 / 280.6	464	K126K (AAA→AAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (165/168); new base C (59/64); total (227/237)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 472 total aligned reads displayed.

ATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGA  >  CP000730/36036‑36313

cagACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                            <  2:17390/138‑1 (MQ=255)

 tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGttt                                                                                                                                           <  1:391978/140‑1 (MQ=21)

  gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACCGTCCGTTTTAAGTTTAAAAGCTATAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTa                                                                                                                                          >  1:319011/1‑140 (MQ=11)

   aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                         <  1:568981/140‑1 (MQ=255)

    cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                        >  1:92313/1‑140 (MQ=18)

    cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACATTCGTTGAAGGTGCCTGATAGGTAATTGCCTGTTGAGGTTGAcc                                                                                                                                        >  2:585031/1‑140 (MQ=11)

       tGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAATCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                     <  1:197614/140‑1 (MQ=255)

        gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAt                                                                                                                                    <  2:449389/140‑1 (MQ=11)

        gATCCTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCGGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                                    >  1:452682/1‑140 (MQ=255)

          tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaattgt                                                                                                                                  >  2:256950/1‑136 (MQ=255)

          tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                                  >  1:105324/1‑140 (MQ=16)

          tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                                  >  1:134703/1‑140 (MQ=16)

          tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGTCTTACCAAATTg                                                                                                                                  >  1:509691/1‑140 (MQ=11)

           cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                 >  1:411095/1‑140 (MQ=16)

           cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                 >  1:539159/1‑140 (MQ=16)

           cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                 >  1:470010/1‑140 (MQ=12)

           cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGCCAGCCTTAAATTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAttct                                                                                                                                 >  1:292923/1‑138 (MQ=11)

           cTTGCTCAATGAGGGTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCACAgtgt                                                                                                                                 >  2:31405/1‑140 (MQ=11)

              gCTCAATGAGGTTATTCAGATATTTCGATGCACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGACGGTGCCTGATCTGTAATTAACTTTTAAGGTTTACCAAATTGTTTa                                                                                                                              >  2:634560/1‑140 (MQ=11)

              gCTCAAGGATGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGTTATTGCTGTTTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTa                                                                                                                              <  1:44343/140‑1 (MQ=11)

                   aTGAGGTTATTCAGATCTTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGTTTTACCAAATTGTTTAATgag                                                                                                                         <  2:198113/140‑1 (MQ=255)

                   aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                                         <  2:352590/140‑1 (MQ=12)

                   aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                                         <  1:375395/140‑1 (MQ=17)

                   aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                                         <  1:206300/140‑1 (MQ=17)

                    tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCGGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgaga                                                                                                                        >  1:177340/1‑140 (MQ=12)

                     gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                                                                                                                       >  1:545590/1‑140 (MQ=12)

                      aGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                                                                      <  2:576547/140‑1 (MQ=32)

                       ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                     >  2:468624/1‑140 (MQ=16)

                       ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTCCTACATTGCAAGGAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                     >  1:154008/1‑140 (MQ=11)

                          tATTCAGATATTTCTATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTGTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTGTGCGGAATACCAAATTGTTTAATGAGACGTTTg                                                                                                                  <  2:441248/140‑1 (MQ=11)

                            ttCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTAAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                                >  1:187211/1‑140 (MQ=17)

                              cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTCCCAAATTGTTTAATGATACGTTTGATaa                                                                                                              >  1:433582/1‑140 (MQ=255)

                              cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                              >  2:621317/1‑140 (MQ=16)

                                gATATTTCCATGTACAATGACAGGCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGCAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                            <  2:547201/140‑1 (MQ=11)

                                 atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGGTACCTTCGTTGAGGGTGCCTGCTTTGTAATTTCCTTTTGGGGTTTACCAAATTGTTTAATGAGGCGTTTGATAAACg                                                                                                           >  2:329837/1‑140 (MQ=11)

                                 atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                                           <  1:308033/140‑1 (MQ=17)

                                  tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                                          >  1:161965/1‑140 (MQ=12)

                                   atTTCTATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTTGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                         <  1:256572/140‑1 (MQ=11)

                                   atTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                         <  2:592837/140‑1 (MQ=17)

                                   atTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                         <  2:226718/140‑1 (MQ=16)

                                     ttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGCGACGTTTGATAAACGCata                                                                                                       <  1:399241/140‑1 (MQ=255)

                                         aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatctact                                                                                                   >  2:368583/1‑135 (MQ=255)

                                         aTGTACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAACAATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAGCTTTTGAGGTTTACCAAATTGTTTAATGAAATGTTTGATAAAAGAATATGCt                                                                                                   >  2:150132/1‑140 (MQ=11)

                                          tGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                  >  1:624623/1‑140 (MQ=16)

                                          tGTACAATGGCAGTCAGGATTAAGTTTAAAGGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTTAGGTTTGCCAAATTGTTTAAGGAGACATTCGATAAACGAATatgatg                                                                                                  >  2:300642/1‑140 (MQ=11)

                                          tGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                  <  2:217655/140‑1 (MQ=255)

                                            tACAATGGCAGTCTGTTTTACATTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGTCTGATCTGTAATTAAATTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                                <  1:245415/140‑1 (MQ=11)

                                             aCAATGACAGTCAGGTTTAAGTTTAACAGCTTTAATTATCTTAGACATTGCTGCGTTCGTTCGCGGTCTACGGTCTGTAACTTCCGTTGCAGGCTCAGCAAATGGTGTATTGATACGTTTTATAAACGCAGATGCTGAAt                                                                                               >  2:487761/1‑140 (MQ=2)

                                             aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                               <  1:75918/140‑1 (MQ=11)

                                              caatgtgcgtgaGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTCTTGAGCTTTACCAATTGGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                              <  2:419179/129‑1 (MQ=1)

                                                 tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                                           >  1:629481/1‑140 (MQ=11)

                                                  gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTCGACATTTCTTCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa                                                                                          <  1:620453/140‑1 (MQ=11)

                                                                       aaccTTTAATTACTGTAAACATTTCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCaa                                                                     <  1:531751/137‑1 (MQ=1)

                                                                                      tAGCCATTGCTACCTTCTTTGAATGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                      <  1:479824/140‑1 (MQ=14)

                                                                                         ccATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATAGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTccc                                                   <  2:538502/140‑1 (MQ=11)

                                                                                           aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                 >  2:380742/1‑140 (MQ=32)

                                                                                            ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                                >  2:83042/1‑140 (MQ=32)

                                                                                               atACTTTCGTTTGATGTGCCTTATTTGTAATTTCTTTTTGAGCTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                             <  1:230816/139‑1 (MQ=11)

                                                                                                tgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                            >  1:585549/3‑140 (MQ=255)

                                                                                                tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                            <  1:168970/140‑1 (MQ=33)

                                                                                                tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                            <  2:134703/140‑1 (MQ=33)

                                                                                                tACCTTCGTTGAAGGTGCCTGATCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAAAAGCAAATGAATGTCCttccgtac                                            >  2:494185/1‑135 (MQ=11)

                                                                                                tACCGTCGTTGAGGGTGCCTGTTCGGTAATTACTTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                            <  2:519602/140‑1 (MQ=11)

                                                                                                 gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                           >  2:65136/2‑140 (MQ=255)

                                                                                                 gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTCGGGCTTAGCAAGTTGTTTAATGAGACGTTTGATAAAGGCATATGCTGAATGATTAACTCGTTACTTACGCAACCGAATATTTAATTTATGTcctcctggata                                           >  2:281771/2‑131 (MQ=11)

                                                                                                 aCCTTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                           <  1:275006/140‑1 (MQ=18)

                                                                                                  ccTTTGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                          <  2:29451/140‑1 (MQ=16)

                                                                                                  ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGTTAAACGCATATGCTGAATGATTTTTTTGTTGCTTACGCAACCAAATATCTAATGTATGTCCTTCTGCATCa                                          >  1:644537/1‑140 (MQ=11)

                                                                                                  ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                          <  2:599234/140‑1 (MQ=33)

                                                                                                   ctaccttGAAGGTGCCGTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                         <  1:111550/135‑1 (MQ=255)

                                                                                                       gtggAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGc                                     <  2:395704/137‑1 (MQ=18)

                                                                                                        ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGTGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                    <  1:22527/140‑1 (MQ=16)

                                                                                                        ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTATGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCa                                    <  1:343823/140‑1 (MQ=18)

                                                                                                        ttGAAGGTGCCTGATCTGTAACTTCATTTTGTGGCTTACAAAATTGTTTAATGAGGCGTTTGATAAACGCATATGTTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                    <  1:575839/140‑1 (MQ=11)

                                                                                                        ttGAAGGTGCCTGATCTGTAAATTCCTTTTGAGGCTTATCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                    <  1:141195/140‑1 (MQ=11)

                                                                                                            aGGTGCCTGATCTTTAACTACCTTTTGGGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCCTCTTTATCCATGGCACTat                                >  1:489932/1‑140 (MQ=11)

                                                                                                            aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGGATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCGTGCATCAATGGCACGat                                >  2:336495/1‑140 (MQ=12)

                                                                                                             ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAACGATACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAAACAAATATATAATGTATGTCCCTCTGTATCAATGGCACGata                               >  1:351457/1‑140 (MQ=11)

                                                                                                              gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCATCTGCATCAATGGCAAGatat                              >  2:242180/1‑140 (MQ=17)

                                                                                                                gCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaa                            >  1:666205/1‑140 (MQ=32)

                                                                                                                 ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaa                           >  1:408911/1‑140 (MQ=17)

                                                                                                                 ccTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaa                           >  1:36623/1‑140 (MQ=255)

                                                                                                                  cTGACCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt                          <  2:529352/140‑1 (MQ=18)

                                                                                                                   tGATCTGTAATTACCTTTTGAAGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTCTCTCGTTGCTTACGCAACCAAGTATCTACTGTATGTCCCTCTGCACCAAGGGCACGATATGAATa                         >  2:458453/1‑140 (MQ=11)

                                                                                                                     aTCTGTCATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                       <  2:233684/140‑1 (MQ=18)

                                                                                                                     aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAACTAGc                       >  2:65655/1‑140 (MQ=17)

                                                                                                                     aTCTGTAACTTCCTTTTGAGGCTTACCAAATTGTTTAATGTGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGATTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                       <  1:553915/140‑1 (MQ=11)

                                                                                                                      tCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                      <  2:506166/140‑1 (MQ=255)

                                                                                                                       cTGTAATTCCATTTTGAGGTTTACCAACATGTTTAATGAGACGTTTGATAAACGCATCTGCTGAATGATTATCTCGTTGCTTACGCAAACCAATATCTAATGTATGTCCATCTTTATCAATGGCACGCTCTACATAgacc                     >  2:119877/1‑137 (MQ=11)

                                                                                                                        tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc                    <  2:111133/140‑1 (MQ=33)

                                                                                                                          tAATTACCTTTTGAGGTTTACCAAACTGTTTAATGAGACCTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                  <  2:404426/140‑1 (MQ=12)

                                                                                                                           aaTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCCCGATATACATAGCGCCaat                 >  2:608582/1‑138 (MQ=255)

                                                                                                                              tACCTTTTTAGGTTTACCAAATTGTTTAATGAGTCGTTTGATTAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCGAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc              <  1:579586/140‑1 (MQ=255)

                                                                                                                                     tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt       <  1:662456/140‑1 (MQ=18)

                                                                                                                                     tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGACACGATATTAATAGGTCccttttccttttct       >  2:248301/1‑138 (MQ=11)

                                                                                                                                      gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt      <  1:120937/140‑1 (MQ=18)

                                                                                                                                      gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt      <  1:297891/140‑1 (MQ=33)

                                                                                                                                      gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAAACCTCCATTTACCTTTTAtt      >  2:68217/1‑140 (MQ=12)

                                                                                                                                      gAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCTACCATATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt      <  1:423473/140‑1 (MQ=255)

                                                                                                                                        ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt    <  1:608593/140‑1 (MQ=33)

ATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGA  >  CP000730/36036‑36313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`