BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	56.2%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	56.2%	85.3 / inf	593	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (119/139); new base T (144/186); total (266/327)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 603 total aligned reads displayed.

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36130‑36408

  tgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                                                                                                                           >  1:585549/3‑140 (MQ=255)

  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                                                                                                                           >  2:220533/1‑140 (MQ=32)

   aCCTTCGTTGAAGGTGCCTGATATGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTACGGCACTCTGCATc                                                                                                                                          >  2:41325/1‑140 (MQ=255)

    ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTTCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                                                                                                                         >  1:179167/1‑140 (MQ=17)

    ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGTGACGTTTGATAAACGCATATGCTAAATGATTATCTCGTTGCTTACGCAACCAAATATCTTATGTATGTCCCTCTGTATCa                                                                                                                                         >  1:139585/1‑140 (MQ=11)

     cTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTAAAGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCGACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                        >  2:27618/1‑140 (MQ=11)

        ctttGAAGGGGCCTGATCTGTAATTACCTTTTTAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATATCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATgg                                                                                                                                     <  2:141332/138‑1 (MQ=11)

          ttGAAGGTGCCTGTTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGTTTAATTATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCa                                                                                                                                   <  1:2312/140‑1 (MQ=255)

          ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                                                                                                                   <  2:590772/140‑1 (MQ=255)

              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATGAGACGTTTGATAAACGCATATGCTGAATAATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGAAAGat                                                                                                                               >  1:128857/1‑140 (MQ=11)

              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGGATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCGTGCATCAATGGCACGat                                                                                                                               >  2:336495/1‑140 (MQ=12)

              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGAATGTCCCTCTGCATCAATGGCAAGat                                                                                                                               >  1:165868/1‑140 (MQ=17)

                 tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATATCGTTGCTTACGCAAGCAAAGATATAATGTATGTCCATCTGATTCAATGTAACGAtata                                                                                                                            >  2:61679/1‑140 (MQ=11)

                   ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaa                                                                                                                          >  1:408911/1‑140 (MQ=17)

                   ccTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTGTATGTCCCTCTGTATCAATGGCACGATATaaa                                                                                                                          >  2:46657/1‑140 (MQ=255)

                     tGATCTGTAATTACCTTTTGAAGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTCTCTCGTTGCTTACGCAACCAAGTATCTACTGTATGTCCCTCTGCACCAAGGGCACGATATGAATa                                                                                                                        >  2:458453/1‑140 (MQ=11)

                     tGATCTGTAACGACCTTTTGAGGCTTACGAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAGTa                                                                                                                        <  2:659672/140‑1 (MQ=11)

                       aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACTAAATATCTAATGTATGTCCCTCTGTATCAATGGGACGATATAAATAGc                                                                                                                      <  1:435336/140‑1 (MQ=11)

                        tCTGTAATTACCTTTTGAGGTTTACCAATTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                                                                                                                     <  2:429373/140‑1 (MQ=12)

                         cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                    <  1:532085/140‑1 (MQ=18)

                             aaTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                                                                                                                <  2:542351/140‑1 (MQ=18)

                                  ccTTTTGAGGTTTTCGATATTGATCAATGTGACGTTTGATTAACTCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGTTATAAATAGCTCCATTTTCCt                                                                                                           <  1:345127/140‑1 (MQ=11)

                                   cctttGAAGCTTTCCAAATTGTTTAATGTTACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                          <  1:335411/138‑1 (MQ=11)

                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                          <  1:117849/140‑1 (MQ=17)

                                      ctcagtcttACCAAATTGTTTGATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa                                                                                                       <  2:423796/133‑1 (MQ=255)

                                       tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt                                                                                                      <  1:662456/140‑1 (MQ=18)

                                        gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAGTGTTGGTCCCTCTGCAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     <  2:488089/140‑1 (MQ=11)

                                        gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     <  2:250437/140‑1 (MQ=18)

                                        gAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCTACCATATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     <  1:423473/140‑1 (MQ=255)

                                        gAGGCTTACCAAATCGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     >  2:608251/1‑140 (MQ=255)

                                         aggcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCACTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt                                                                                                    >  1:621854/5‑140 (MQ=12)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt                                                                                                   <  2:51200/140‑1 (MQ=33)

                                           gTTTACCAAATTGTTTAATGAGACGTTTGGTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTCATGTATGTACCTCTGTCTCAATGGCACGATGTACATTACTCCACTTTCCTCTTCTTTTg                                                                                                  >  2:497715/1‑140 (MQ=11)

                                           gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  <  2:170992/140‑1 (MQ=17)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCGACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATCTAAATAGCTCCATTTTACTTTTCTCTTGAt                                                                                                >  2:551904/1‑140 (MQ=11)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                >  1:578558/1‑140 (MQ=17)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                <  2:448303/140‑1 (MQ=33)

                                               aCCAAATTGTTTAATGAGCCGGTGGATAAAAGCATACGCTGAATGGTTTTCGCGTGGCTTACGAAACCAGATATATAATTTATGTCCCTCGGTATGAAGTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                              <  2:57695/140‑3 (MQ=2)

                                                ccAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATTGCTCCATTTTCATTTTATTTTAAtata                                                                                             >  2:114902/1‑140 (MQ=11)

                                                ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtata                                                                                             <  2:181517/140‑4 (MQ=16)

                                                    aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                         <  1:362737/140‑1 (MQ=32)

                                                        tttAATGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                     <  1:444491/140‑1 (MQ=17)

                                                        tttAATGAGACGTTTGATAAACGCATATGCTGGATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                     <  1:609582/140‑1 (MQ=255)

                                                        tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                     <  1:35419/140‑1 (MQ=16)

                                                        tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                     <  1:595728/140‑1 (MQ=32)

                                                         ttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatc                                                                                    >  1:388669/1‑140 (MQ=17)

                                                         ttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAGTTTGATGTACGTCtcatc                                                                                    >  1:395976/1‑140 (MQ=17)

                                                          tcaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatca                                                                                   <  2:581164/138‑1 (MQ=18)

                                                          tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatca                                                                                   <  1:296946/140‑1 (MQ=32)

                                                          tAATGAGACGTTTGATAAACGCATATGCTGAAGTATTATCTCGTTGCTTACGCAGCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAGATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatca                                                                                   <  2:365709/140‑1 (MQ=11)

                                                            aTGAGACGTTTGCTAAAAGCATATGCTCAATGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                 <  2:154008/140‑1 (MQ=255)

                                                            aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCGAATGTATGTACCTCTGTATCCATGGCACGATATACATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                 >  2:203162/1‑140 (MQ=11)

                                                             tGAGACGTTTGATAAACTCATATGTTGTATGATTATCTTTTTGCTTACGCAACCAATTATCTAATGTATGTTCCTCTGTATCAATGGTATGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                <  1:308175/140‑1 (MQ=11)

                                                               agaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCCGTATCAATGGCACGCTATCAATAGCCCCACTTACCTAATATTTTGATATTCTTCTCATCAATACg                                                                              <  2:321895/140‑1 (MQ=11)

                                                                   gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTACATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAt                                                                          >  2:382607/1‑140 (MQ=12)

                                                                   gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGACACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGGCTCATCAATACTCCaa                                                                          >  1:48495/1‑139 (MQ=12)

                                                                   gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAAAATATCAACCTGGTTTCTTTTCTTCTATTGCCACACATAAATAGCTCCCGTTTCCTTTTATTTTGATGTACGTCTCATCaaacccccgt                                                                          >  2:128/1‑132 (MQ=2)

                                                                    tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCACCATTTTCCTTTTATTTTGATTTACGTCTCATCAATACGCCAtt                                                                         >  1:249722/1‑140 (MQ=12)

                                                                     ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAttt                                                                        >  2:357159/1‑140 (MQ=33)

                                                                         tcaaCGCATATGTTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGTTATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                    <  1:198114/138‑1 (MQ=12)

                                                                         tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                    <  1:220533/140‑1 (MQ=32)

                                                                          aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                   >  1:21134/1‑140 (MQ=12)

                                                                           aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                  <  2:37398/140‑1 (MQ=33)

                                                                            aCGGATATGCTGAATGATTATCTCGTTGCTTACGCATCCAAATATCTAAGGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataa                                                                 <  1:256280/140‑1 (MQ=11)

                                                                            aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataa                                                                 >  1:565244/1‑140 (MQ=19)

                                                                            aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTACTTTTATTTTTATATACGTCTCATCAATACGCCATTTGtaataa                                                                 >  1:633035/1‑140 (MQ=255)

                                                                                 tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGAttt                                                            >  1:103655/1‑140 (MQ=25)

                                                                                    tgtGAATGATTATCTCTTTGCTTACGCAACCAACTCTCTAATGTATGTCCCTCGGTATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACTTCATTTGTAATAAGCtttttt                                                         <  2:625125/138‑1 (MQ=11)

                                                                                     actaataatGATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                        <  2:438387/137‑1 (MQ=11)

                                                                                      tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAATTTGTAATAAGCtttttttt                                                       >  1:644102/1‑138 (MQ=255)

                                                                                       taaTGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                      <  2:290341/139‑1 (MQ=255)

                                                                                        aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                     <  1:618465/140‑1 (MQ=255)

                                                                                         aTGACTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                    <  2:246376/140‑1 (MQ=255)

                                                                                             ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                >  2:654236/1‑140 (MQ=40)

                                                                                               aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                              <  2:388475/140‑1 (MQ=255)

                                                                                                tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                             >  2:521586/1‑140 (MQ=40)

                                                                                                 ctcGTTGCTTTCGTAAGCAAATACCTAATGTATTTCCCTCTTTATCAATGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                            <  1:150132/140‑1 (MQ=11)

                                                                                                    gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCaa                                         >  2:532718/1‑140 (MQ=14)

                                                                                                      tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTACGTTCTTTCTGCGAAAt                                       <  2:309305/140‑1 (MQ=14)

                                                                                                          taagCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACATCTCATCAATACGCCATTTGTAATAAACTTTTTTTTGCTTTTTATTCCAAGTTTGa                                   >  1:547315/4‑140 (MQ=14)

                                                                                                           aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTgct                                  >  2:162962/1‑138 (MQ=255)

                                                                                                            cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTTTAAAtttaaaa                                 >  1:96556/1‑136 (MQ=14)

                                                                                                             gccaCCAAAGATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                <  2:630728/137‑2 (MQ=255)

                                                                                                             gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                <  1:76923/140‑2 (MQ=255)

                                                                                                               aaCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                              <  2:644537/140‑1 (MQ=38)

                                                                                                                aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCTCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACaaa                             <  1:318896/140‑1 (MQ=255)

                                                                                                                aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTACGCTTTTTCTTCCAAATTTGATATaaa                             <  2:400025/140‑1 (MQ=255)

                                                                                                                  cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTCTTAGAAGAATTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                           >  1:513952/1‑140 (MQ=11)

                                                                                                                    aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGCCTCATCAATACGCCATTTGTATTAAACGTTTTTATCGTTTTTCTTCCAAATTTGATGTAAACTTg                         >  1:561064/1‑140 (MQ=11)

                                                                                                                     atatCTAATGTATGTCCCTCTGTATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                        <  1:493664/140‑1 (MQ=11)

                                                                                                                     ataaCTAATGTATGTCCCCTCGCATACAAGGCACGATATAAATAGCTCCATTTTTCTTTTATTTTGATGTACGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTgg                        <  2:124506/140‑1 (MQ=14)

                                                                                                                         cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                    <  2:10592/140‑1 (MQ=37)

                                                                                                                          tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                   <  1:274797/140‑1 (MQ=255)

                                                                                                                          tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                   <  1:368405/140‑1 (MQ=255)

                                                                                                                          tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCACAATTTTCCTTTTAGTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACATTTTTTTGCTTTTTCTTGAAAATTTGATTTAAAATTAGGGcct                   >  2:238423/1‑138 (MQ=11)

                                                                                                                            atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTAATTTACGTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTGATATAAAATTGGGGCctat                 >  1:465795/1‑140 (MQ=11)

                                                                                                                             tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                <  2:666491/140‑1 (MQ=255)

                                                                                                                                   tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGaa          <  1:242239/140‑1 (MQ=40)

                                                                                                                                       tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCTTCCAAATTTAATAAAAAATTGGGGCATATTCTTGAACCCa      >  1:604345/1‑140 (MQ=255)

                                                                                                                                           cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATACTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg  >  1:280412/1‑140 (MQ=21)

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36130‑36408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`