Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 40.6% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT40.6% 96.3 / 94.7 105intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/34);  new base T (21/21);  total (49/56)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 105 total aligned reads displayed.

AATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTT  >  CP000730/36448‑36718
                                                                                                                                     |                                                                                                                                         
aaTATTTCATATATATCAAGATAATGCAATGTATATCTTAGATTGTAGTTAATGTCTTCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                                                                                                     <  1:18589/140‑1 (MQ=11)
aaTATTTCAGAGATATCATGATAACTCAATGTATATCTTAGATAGTAGTCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                                                                                                     <  1:121266/140‑1 (MQ=255)
 atatTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAt                                                                                                                                    <  2:562477/140‑1 (MQ=34)
          atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg                                                                                                                           <  2:171479/140‑1 (MQ=35)
            atatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                                                                                                         <  2:45790/140‑1 (MQ=35)
              atCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAc                                                                                                                       <  1:96730/140‑1 (MQ=21)
                 aCGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCCTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGa                                                                                                                    <  2:94054/140‑1 (MQ=255)
                   gcaaaCACAATGCATATCTTAGAAAGTAGCCAAACGCTACAGCGATACCATCCTTGTTAAATTCTTTATATCTGCAATAGTTCATACAGATGACTCCTTTATGTTAAAATTATACTATTAATTCAACTTTGCAACAgccc                                                                                                                  <  2:297821/137‑4 (MQ=255)
                    taaaCTCTATGTATATCTGAGTTATTAGCCGACGTCGACCGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTCTAAATTCCACTTTGCAACAGAAcc                                                                                                                 <  2:313885/138‑1 (MQ=11)
                      agcTCAATGCATATCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGATATAGTTCATACAGAAGTTTCCTTTTTGTTAAGATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               <  1:510464/138‑1 (MQ=11)
                      agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTAGTTAAATAGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               >  1:201148/3‑140 (MQ=11)
                       gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGAAACAGAACCGta                                                                                                              >  1:176325/2‑140 (MQ=12)
                       aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGACCCGta                                                                                                              >  2:345241/1‑140 (MQ=38)
                       aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta                                                                                                              >  2:335032/1‑140 (MQ=39)
                        cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTTAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             >  2:133445/1‑140 (MQ=255)
                        cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             >  1:165123/1‑140 (MQ=12)
                        cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             >  2:407495/1‑140 (MQ=16)
                        cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCATTTTTGTTAAAATTATATTATAAATTCAAATTTGCAACAGAACCGtat                                                                                                             >  1:652670/1‑140 (MQ=255)
                         tcaagtcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctccta                                                                                                            <  2:278809/133‑6 (MQ=11)
                          cAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                           >  1:217589/1‑140 (MQ=255)
                           aaTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATCTCTGAAATAGTTCATACAGAAGAATCCTTCCGATTAAAATAATATTATACATGCAACATTGCAACAGAACCGtattat                                                                                                          >  1:313865/1‑140 (MQ=11)
                           aaTGGATATCTTAGATAGTAGCCAACGGCTACAGTGATAAGATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAATAGAACCGtatttt                                                                                                          >  1:476929/1‑138 (MQ=11)
                            atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTCTGCAACAGAACCGtatttta                                                                                                         <  1:403360/136‑4 (MQ=11)
                             tggatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAATCTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGGAACAGAAcctcctaactg                                                                                                        <  1:523037/137‑10 (MQ=11)
                              gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTTTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                       <  1:266827/138‑6 (MQ=11)
                              gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTATAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                       <  1:205793/138‑6 (MQ=11)
                              gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                       <  2:662111/138‑6 (MQ=255)
                              gcatatCTTAGATAGTAGCCAACAGCTACAGTGATAACACCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttacta                                                                                                       >  1:223220/3‑130 (MQ=1)
                              gcatatCTGTGATAGTAGCCAACGGCTACAGTGATAACATCCTTTTTAAATTGTTTATATCTGAACTAGTTCATAGAGAAGGCACGTTTTGGTAAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                       <  2:61228/138‑6 (MQ=11)
                              gcagatCTTGGATAGTAGCCAACAGCTACAGTGATAATATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttacta                                                                                                       <  2:375577/136‑11 (MQ=11)
                               catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATTCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgta                                                                                                      >  2:376878/2‑129 (MQ=11)
                               catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgta                                                                                                      <  2:214248/139‑12 (MQ=11)
                               catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                      <  1:248386/139‑7 (MQ=255)
                                atttCTTAGATAGTAGCCAACGGCTACAGTGATAACCTCCTTTTTCAATGTCTTATATCTGAAATAGTTCATACAGTAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCATCAGATCCGtattttatgag                                                                                                     <  2:147891/137‑8 (MQ=11)
                                atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctactaactgtat                                                                                                     >  2:256609/1‑128 (MQ=11)
                                atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactata                                                                                                     >  1:547735/1‑128 (MQ=11)
                                atatCTTACATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAAACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                     <  2:147980/140‑8 (MQ=11)
                                atatCTGAGAAAGTAGCCAACGGCTACAGTGATCACATCCAAGACACGTTCTATATCTCTGAAATAGTTAATACAGATGGCTCCATTTTGTTAAAATTATATTATGAATTAAACTTTACACCAGAccctcctagctgtat                                                                                                     >  2:79602/1‑128 (MQ=2)
                                 tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                                                                                                    >  2:4143/1‑127 (MQ=11)
                                 tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                                    <  1:464694/140‑1 (MQ=255)
                                 tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACGTTGCAACAGAACCGTATTATGGAATa                                                                                                    <  1:51894/140‑1 (MQ=255)
                                 tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGTTTTCTTTTTGTTAAAATTATACTATAAATGCAACTTTGCAACAGAGcctcatttactatat                                                                                                    <  1:63110/140‑14 (MQ=11)
                                 tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                    <  1:219227/140‑14 (MQ=11)
                                 tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                    <  1:358985/140‑14 (MQ=11)
                                  atCTTAGATAGTAGCCAACGGCTACAGTGATAAGATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAACGtattttatgagta                                                                                                   >  1:140938/1‑131 (MQ=11)
                                  atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                                                                                                   >  1:115313/1‑126 (MQ=11)
                                  atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                   >  1:644896/1‑131 (MQ=255)
                                  atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTAAAAATTCAACTTTGCAACAGAACCGtattttattagta                                                                                                   >  1:146566/1‑131 (MQ=11)
                                  atCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGAAcctcttttactatatg                                                                                                   >  2:36905/1‑126 (MQ=11)
                                  atCTTAGATAGTAGCCAACAGCTACAGTCATAACATCCTTGTTAAATAGTTTATCTCTGAAATAATTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTATGCAACAGAAcctcatttactatatg                                                                                                   >  1:430172/1‑126 (MQ=11)
                                  atCTGAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                                                                                                   <  2:513869/140‑15 (MQ=11)
                                   tCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTCATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAGTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                                                                                                  <  2:377702/140‑11 (MQ=11)
                                   tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                                                                                                  >  2:475420/1‑130 (MQ=11)
                                    cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca                                                                                                 <  2:274664/140‑12 (MQ=11)
                                     ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacac                                                                                                <  1:478917/140‑13 (MQ=11)
                                      tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                               <  1:287331/140‑14 (MQ=11)
                                      tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt                                                                                               >  1:173918/1‑140 (MQ=255)
                                       aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                              >  2:393736/1‑126 (MQ=11)
                                       aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                              <  1:262311/140‑15 (MQ=11)
                                       aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttataaaaacacac                                                                                              <  2:60426/140‑15 (MQ=11)
                                           agtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTgg                                                                                          >  2:478246/1‑140 (MQ=255)
                                              agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACTGTATTACGGAATAGAGATATTGATaa                                                                                       >  2:135387/1‑140 (MQ=255)
                                              agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                       >  1:367217/1‑140 (MQ=255)
                                              agCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                       <  2:311526/140‑1 (MQ=255)
                                                 cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAATTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAt                                                                                    <  1:521121/140‑1 (MQ=255)
                                                    cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtttt                                                                                 >  2:181122/1‑139 (MQ=255)
                                                      gCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAACTTCACCTTTTCAACAGAACCGTATTACGGAATAGAAATGTTGGTAACACTtata                                                                               >  2:23400/1‑140 (MQ=17)
                                                        tACAGTGATAACATCCTTGTTAAATTGTTTATATCTCAAATAGTTGACACACAAGACTCCTGTTGCTTAAAATTAAACTATAAATTAAACTTTGCCGCAAAACTGTGTTGTGGAATAGAGCTGTTTGTAACATTTTTACa                                                                             >  2:270777/1‑140 (MQ=9)
                                                         aCAGTGATAACATCCTTGTTAAATTGTTTGTATCTGAAATAGTTCATAAAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAAATGTTGGCAACATTTATACAg                                                                            >  1:99526/1‑140 (MQ=255)
                                                         aCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAATATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAg                                                                            >  1:231763/1‑140 (MQ=255)
                                                          cAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATTGAGATGTTGTTAACATTTATACAgg                                                                           <  1:335032/140‑1 (MQ=255)
                                                             tGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATc                                                                        >  1:306133/1‑140 (MQ=255)
                                                             tGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTATTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATGATGGAATAGAGATGTGGGTAAAATTTATACAGGATc                                                                        >  1:237797/1‑140 (MQ=255)
                                                             tGATAACATCCTTGATAAATTGTTTATATCTGAAATAGTTCATACAGAAGATTCCTTTTTGTTAAAATTATACTATAAATTCCACTTTTCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATc                                                                        <  1:28334/140‑1 (MQ=255)
                                                                   cATCCTTGTTAAATAGTTTGTATCTAAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATtata                                                                  >  1:365749/1‑140 (MQ=255)
                                                                          gTTAATTTCTTTATATTTGAAATAGTTCATACAGAATACTCCTGTTTGTTAAAATTATACTATAAATTCTACTTTGCAACATAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGt                                                           <  2:121980/140‑1 (MQ=25)
                                                                           ttAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                          <  2:530069/140‑1 (MQ=255)
                                                                                ttGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTTTGGTCTAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                     <  1:519474/140‑1 (MQ=255)
                                                                                ttGTTTATATCTGAAATACTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                     <  2:284007/140‑1 (MQ=255)
                                                                                gtttttATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                     <  2:563857/137‑1 (MQ=255)
                                                                                  gTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTc                                                   <  2:385044/140‑1 (MQ=255)
                                                                                       tatCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttat                                              <  2:182491/140‑1 (MQ=255)
                                                                                       tatCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTACAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTGTAATTTCGttat                                              >  1:378972/1‑140 (MQ=255)
                                                                                                  gTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcac                                   >  2:595037/1‑140 (MQ=255)
                                                                                                   ttCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGCTGGTAACACTTACACAGGACCATTATACTTAAGTCTAATTACGTTATTACAGagtcata                                  <  2:389170/140‑7 (MQ=25)
                                                                                                    tCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGAATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacac                                 >  2:462408/1‑140 (MQ=255)
                                                                                                     cATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacaca                                >  1:661107/1‑140 (MQ=255)
                                                                                                        ccacccgcaaccTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTc                             <  2:465172/130‑1 (MQ=255)
                                                                                                         cAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTcc                            <  1:638164/140‑1 (MQ=255)
                                                                                                                cTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCaga                     <  2:582957/140‑1 (MQ=255)
                                                                                                                cTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCaga                     <  1:491271/140‑1 (MQ=255)
                                                                                                                 tCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCagaa                    <  2:14435/140‑1 (MQ=255)
                                                                                                                 tCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAACTTCCATCTCTCAGAACCACACATTCCAACCagaa                    <  1:659706/140‑1 (MQ=38)
                                                                                                                 tCCGTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCagaa                    <  2:14716/140‑1 (MQ=255)
                                                                                                                  ccTTTTTTTTATTTTTATACTATAAATTCAACTTTGCAACAGAACGGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCAGTATACTTAAGTTTATTTTCGTTATTAGAGAAACAAACATTCCAACCagaag                   >  2:410013/1‑140 (MQ=255)
                                                                                                                  ccTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACCTTCCAACCagaag                   >  1:262481/1‑140 (MQ=255)
                                                                                                                     ttatggtAAAATTATACTATAAATTCTACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTAGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAgaga                <  2:442741/134‑1 (MQ=255)
                                                                                                                          ttAAAATTATACTATAAATTCAACTTTGCACCCGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACCGAACCACACATTCCAACCAGAAGAAAAAGTa           >  2:274576/1‑140 (MQ=255)
                                                                                                                                  ataCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTagttcaatt   >  2:571537/1‑132 (MQ=255)
                                                                                                                                   taCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCACAAGAGGAAGTAGGtgacttt  >  2:355035/1‑134 (MQ=255)
                                                                                                                                     |                                                                                                                                         
AATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTT  >  CP000730/36448‑36718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: