Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 54.7% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC54.7% 104.7 / inf 473intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (108/103);  new base C (136/118);  total (250/223)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

ATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCG  >  CP000730/76156‑76434
                                                                                                                                           |                                                                                                                                           
tgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                                                                                                                                             <  2:476766/138‑1 (MQ=9)
ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGAAATATTATGTATGAAATATTTAAGATATAAAAAATTTAAAAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGATATGCATTGAGt                                                                                                                                             >  1:188018/1‑140 (MQ=11)
    gTTCTGTTGCAAAGTTGAATATATAGTATAATTTTAACAAAAAGGAGTCTACAGTATGAACTATTTCAGATATAAACAATTGAACAAGAATGTTATCACTGTAGCAGTTGGCTACTAACTAAGATATGCATTGAGTAATc                                                                                                                                         >  2:71773/1‑140 (MQ=11)
      tCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCCTCTGTATTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                                                                                                                                       <  1:492048/140‑1 (MQ=255)
       cTGTTGCAAAGTTGAATCTATAGTAAAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTTAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTg                                                                                                                                      <  1:634008/140‑1 (MQ=25)
        tGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                                                                                                                                     <  2:384727/140‑1 (MQ=37)
                      atttataatataaTTTTAACAAAAAGGCAACTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAatat                                                                                                                       >  2:610648/4‑140 (MQ=11)
                      atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAatat                                                                                                                       >  2:402950/4‑140 (MQ=0)
                       tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                      <  2:218442/138‑1 (MQ=9)
                       tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg                                                                                                                             <  1:84463/133‑1 (MQ=34)
                         tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGCGCTATCGTGATATATCTGAAATATTaa                                                                                                                    >  2:175440/1‑140 (MQ=255)
                         tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTGCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATAGGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                    >  2:167928/1‑140 (MQ=255)
                            aatataATTTTAACAAAAAGGAGTCTTGTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgag                                                                                                                 <  2:100810/138‑3 (MQ=12)
                            aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTGAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgag                                                                                                                 <  1:419350/138‑3 (MQ=255)
                                taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                                             >  1:100391/1‑140 (MQ=12)
                                 aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgt                                                                                                            <  1:619054/140‑1 (MQ=17)
                                 aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                                                            >  1:588452/1‑140 (MQ=12)
                                   ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCCGATATAAACATTTTAATCAGGCTGTTTTCACTGTAGCCGTTGGCTAGTAGCTAAGGTATGTATTGAGGTAGTGTGAGAGAGCGGAAATATTAAGGTAACttgg                                                                                                          <  2:132038/140‑1 (MQ=2)
                                   ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                                                          <  2:391951/140‑1 (MQ=33)
                                     ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTCACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                                                        <  2:535404/140‑1 (MQ=255)
                                     ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCATTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                                                        <  1:277145/140‑1 (MQ=255)
                                     ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                                                        <  1:478863/140‑1 (MQ=16)
                                       aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                                                      >  2:321978/1‑140 (MQ=17)
                                        aCAAACAGGAGACTGCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGACGATATCACTGTAGCAGTTCGCAACTAACTAAGATATGCATTGAGTTATCGTGATATATCTGAAATGTTAAGGGGACGTCGTGTaa                                                                                                     >  2:451721/1‑140 (MQ=11)
                                        aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                     >  1:508945/1‑140 (MQ=17)
                                        aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                     >  2:3546/1‑140 (MQ=18)
                                           aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATAGAGTTATCGTGATATATCTGAAACATTCAGAGAACATGTTGTAAACg                                                                                                  >  1:250033/1‑140 (MQ=11)
                                            caaGGAGTCTTCTGTATGAACGATTTCAGATATAACCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                 <  2:585203/139‑1 (MQ=11)
                                            aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAGTTAACGAGGATGTTATAACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                 >  1:320295/1‑140 (MQ=11)
                                            aaaGGAGTCTTCTGTATGAACTATTTAAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGGGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                 >  2:259142/1‑140 (MQ=255)
                                              aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTGTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAACTATCATGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                               >  1:386608/1‑140 (MQ=11)
                                              aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTTAGTTATCGTGATATATTTGAAATAGTAAGGGCACGTGGTGTAAACGTtc                                                                                               >  2:651455/1‑140 (MQ=11)
                                                   tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAAAGAACGTGGTGTAAACGTTcatcat                                                                                          >  2:251056/1‑140 (MQ=16)
                                                    cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCAtt                                                                                         <  1:164787/140‑1 (MQ=16)
                                                      tctGTATGAACTATTTCAGAGATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCa                                                                                       >  1:402337/1‑140 (MQ=17)
                                                        tGTTTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGGTATCGTGATATATCTGAAGTATTAAGGGAACGTGGGGTAAACGTTCATCATTCAAc                                                                                     >  2:280412/1‑140 (MQ=11)
                                                        tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAAc                                                                                     >  2:510001/1‑140 (MQ=17)
                                                        tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGGGGTGTAAACGTTCATCATTCAAc                                                                                     >  2:338796/1‑140 (MQ=12)
                                                        tGTATGAACTAGTTCAGATATAAACAATTTAATAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTCAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTTTAAACGTTCATCATTCAAc                                                                                     <  2:372332/140‑1 (MQ=11)
                                                         gTATTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                    <  2:523037/140‑1 (MQ=12)
                                                          tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                   <  1:549904/140‑1 (MQ=32)
                                                            tGAACTTTTTCAGATATAAACAATTTAACGAGGATGTTATCACTGTAGGCGTTCGGTACGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                 <  1:315792/140‑1 (MQ=11)
                                                            tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAAAGTAGTGTAAACGTTCATCATTCAACGATc                                                                                 >  1:119877/1‑140 (MQ=11)
                                                              aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTa                                                                               >  1:242705/1‑140 (MQ=18)
                                                              aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTa                                                                               <  2:128423/140‑1 (MQ=255)
                                                                cTATTTCAAATATATACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAATATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                             <  2:288582/140‑1 (MQ=12)
                                                                  aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGtagaccgt                                                                           <  2:62503/140‑8 (MQ=255)
                                                                   tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                          >  1:608251/1‑140 (MQ=255)
                                                                    ttCAGATATAAACAATTTAACAATTTAATTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                         >  2:143593/1‑140 (MQ=11)
                                                                     tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                                                        >  1:521586/1‑140 (MQ=17)
                                                                      catatatAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                       <  2:237417/137‑1 (MQ=17)
                                                                       aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                 <  1:157803/129‑1 (MQ=17)
                                                                       aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGt                                                                      >  2:558230/1‑140 (MQ=18)
                                                                          tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                   <  2:543544/140‑1 (MQ=255)
                                                                            taAACAATTTAACAAGTATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                 <  2:593270/140‑1 (MQ=11)
                                                                            taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                 <  2:562546/140‑1 (MQ=255)
                                                                             aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTAAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                >  1:65655/1‑140 (MQ=17)
                                                                              aaCAATTTAACAAGGATGTTAGCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAATGTGCTGTCAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGaa                                                               >  1:345742/1‑140 (MQ=11)
                                                                               accaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                              <  2:463950/137‑1 (MQ=255)
                                                                                 aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTGAGCTATCGTGATATATCTGAAATATTATGAGAACTTGGGGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                            <  1:56896/140‑1 (MQ=11)
                                                                                 aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGTAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAatat                                                            <  2:82917/140‑1 (MQ=17)
                                                                                 aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                            <  2:665956/140‑1 (MQ=255)
                                                                                    ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                         >  1:453553/1‑140 (MQ=17)
                                                                                    ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                         <  2:50029/140‑1 (MQ=255)
                                                                                      aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                       >  2:620042/1‑140 (MQ=255)
                                                                                       aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTGCAAGAATATGCCCCa                                                      >  1:534126/1‑140 (MQ=17)
                                                                                          aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                   >  1:173955/1‑140 (MQ=12)
                                                                                           ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAAAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCACCAAttt                                                  >  1:12591/1‑140 (MQ=11)
                                                                                              tGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                               <  1:68446/140‑1 (MQ=18)
                                                                                              tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                  <  1:286991/137‑1 (MQ=255)
                                                                                               gTTATCACTGTAGCCGTTGGCTACTATCAAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                              >  1:430704/1‑140 (MQ=255)
                                                                                                ttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                             <  1:196252/140‑1 (MQ=25)
                                                                                                 tATCACTGTAGCCGCTGGCTACTATCTAAGATATGCATTGAGCTCTCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                            >  1:364152/1‑140 (MQ=11)
                                                                                                     aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                        >  2:603510/1‑140 (MQ=25)
                                                                                                     aCTGTAGCCGTTGGCTACTATCTCAGATATGCATTGAGTTATCGTGATATATCTGAAATATGAAAGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                        >  1:10739/1‑140 (MQ=255)
                                                                                                      cTGTAGGCGTTGGTGAGTATCTAAGAGATGCATGGCGGTATCGTGATATATCTGCAGTATTAAGGGAACGTGGTGTAAACGTTGATCATCCAACGATCTAGCGTTGGGTTCAATAATATGCCCCAATGTTTTATTAAAtt                                       <  2:118376/140‑1 (MQ=2)
                                                                                                      cTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt                                       >  1:643659/1‑140 (MQ=255)
                                                                                                         taaccGTTGGCTACTATCTAAGAAAAGCATTGAGCTATCGTGATATATCTGAACAATTAAGAGAAAGTGGAGTAAACGTTCATCAATCAACGATCAACAGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtgacg                                    >  2:153087/4‑136 (MQ=11)
                                                                                                           gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAAAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTAGGGTTCAAGAATAAGCCCCAATTTTATATCAAATCTGgaa                                  >  1:146696/1‑140 (MQ=11)
                                                                                                            ctgTTGTCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                 <  2:414824/138‑1 (MQ=25)
                                                                                                            ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                 >  2:429130/1‑140 (MQ=255)
                                                                                                            ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                 <  1:380947/140‑1 (MQ=255)
                                                                                                              aacggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                               >  1:577315/4‑140 (MQ=255)
                                                                                                                  tctactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                           <  2:326770/139‑1 (MQ=255)
                                                                                                                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                           <  1:615768/140‑1 (MQ=37)
                                                                                                                    tactaTCTAAGATATGCATTGAGCTATCTTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                         <  1:211156/140‑1 (MQ=255)
                                                                                                                     actaTCTAAGGTATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                        <  1:515757/140‑1 (MQ=21)
                                                                                                                     actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                        <  2:302362/140‑1 (MQ=35)
                                                                                                                     actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATCTCAAATTTGGAAGAAAAAGCAt                        <  2:643659/140‑1 (MQ=255)
                                                                                                                     actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCAAATTTTATATCAAATTTGGAAGAAAAAGCAt                        >  1:232537/1‑140 (MQ=11)
                                                                                                                       taTCTAAGATAAGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                      <  2:91742/140‑1 (MQ=25)
                                                                                                                        aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                     >  2:633111/1‑140 (MQ=37)
                                                                                                                           tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                  <  1:375577/140‑2 (MQ=21)
                                                                                                                                tgtacATTGAGCTATGGTGATATTTCTAAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCAAATTTTATATAAAATTTGGAAGAAAAAGCATaaaaacacttg             >  2:489932/5‑134 (MQ=11)
                                                                                                                                tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAAt             >  1:446041/1‑140 (MQ=40)
                                                                                                                                  tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTa           >  1:266684/1‑140 (MQ=40)
                                                                                                                                   gCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTACAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGATAATTAc          >  1:466030/1‑140 (MQ=255)
                                                                                                                                   gCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTAAACGGTCTACCGTTGGGTTCAAGCCTATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTAc          >  1:136537/1‑140 (MQ=255)
                                                                                                                                    cATTGAGTTATCGTGATATAGCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGACTCTGCCCCAATTTTATATCAAGTTTGGAAGAAAAAGCATAAAAAGCTAATTACa         >  2:365513/1‑140 (MQ=255)
                                                                                                                                     aTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTTGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACaa        <  2:397033/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCG  >  CP000730/76156‑76434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: