Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,646,1890GC100.0% 178.4 / ‑6.5 63A155G (GCA→GGA) USA300HOU_1531M24B family Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (28/34);  minor base A (0/1);  total (28/35)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAT  >  CP000730/1646053‑1646320
                                                                                                                                        |                                                                                                                                   
tttATCACTTGCAACACCATGTGGTAATGCACATTTATAACCAGATTCGAGAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTCCTTCCTTTTCAGTCATGTCTACtt                                                                                                                                  <  2:467529/140‑1 (MQ=255)
  tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                <  1:231752/140‑1 (MQ=255)
     cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTaca                                                                                                                             <  2:493901/140‑1 (MQ=255)
      acgtGCAACACCATGTGTTAATGCACCTCTATGACCAGAATCTACTTACGTACAGAATGTTGGTAAATCTGCTCCTTCTTCTTGAAGGTTAATTTCTTACAATGCCTTGAGTGCTTTTTCGTTCGTGCCTCCTTTTagaa                                                                                                                            <  2:295213/137‑1 (MQ=255)
       cTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTTCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                           <  1:173975/140‑1 (MQ=255)
       cTTGCAACACCATGAGGTAATGCACCTCTCTGACCAGATGCTAAAATAGTATCGAATGATGGTCACTCAGCTCCTAATTCTAGCATTTTACTTACTATTATTGCCTTTAATTCTTTTTCAGTCATGCCTCATTTTaccca                                                                                                                           >  2:293634/1‑137 (MQ=255)
          gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTGATAGTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                        <  1:187909/140‑1 (MQ=255)
              cacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                    <  1:634106/140‑1 (MQ=255)
                   tgtgGTAATTCACCTCGATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatata                                                                                                               <  1:373223/140‑1 (MQ=255)
                   tgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatata                                                                                                               >  2:378252/1‑140 (MQ=255)
                    gtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAACGCTTTTGCAGTCATGCCTCCTTTTACCAAAGTTAAAatatat                                                                                                              >  2:279942/1‑140 (MQ=255)
                     tgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                                                                             >  1:144242/1‑140 (MQ=255)
                        tAATGCATCTCTATGACCAGATGCTACAATCGTATCTAATGATGGTCCATCTGCTCATTCTATGAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                          <  1:154645/140‑1 (MQ=255)
                        tAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                          <  1:459198/140‑1 (MQ=255)
                         aaTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                         <  1:119073/140‑1 (MQ=255)
                           tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATg                                                                                                       <  1:338845/140‑1 (MQ=255)
                                  ctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTAAAATATATTCATATGTTtcatc                                                                                                >  1:198668/1‑140 (MQ=255)
                                  ctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                                                                >  1:271447/1‑140 (MQ=255)
                                   tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTATAGAATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcttca                                                                                               >  1:454943/1‑140 (MQ=255)
                                   tATGACCAGATGCTACAATCGTATAGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTCATTCTATTTCATTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                               >  2:330077/1‑140 (MQ=255)
                                    aTGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                              <  2:537262/140‑1 (MQ=255)
                                     tGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaac                                                                                             >  1:268557/1‑140 (MQ=255)
                                        ccAGATGCTACAATCGGATCGAATGATGGCCCATCTGCTCCTAATTCTAGCATTTTGATTTATAATATTGCCTTTAATTCTTTTTCAGTCGTGCTTCCTTTTCGAACAGTTAAAATATTTTTATATGTTTCGTAAACTat                                                                                          >  2:151046/1‑140 (MQ=255)
                                               cTACAATCGTATCGAATGATGGTCCATCTGCTACTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATAAATTCATATTTTTGATCAACAATATTAgtt                                                                                   >  2:112363/1‑138 (MQ=255)
                                                 aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATATTTCATCAACAATATTAgctgc                                                                                 >  1:495239/1‑140 (MQ=255)
                                                  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTAAAATATATTCATATGTTTCATCAACAATATTAcctgct                                                                                >  2:356911/1‑140 (MQ=255)
                                                   aaTCGTATCGAATGATGGTCCTTCTGCTCCTAATTCTAGCATTTTGCTTTCGAATATTTCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGCTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                               <  2:22954/140‑1 (MQ=255)
                                                     tCGTATCGAATGATGGTCTATCTGCTCCTATTTCTAGCATTTTGCTTTCTAAATTTGCCCTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTGTCATCAACAATATTAGCTGCtttt                                                                             <  2:621119/140‑1 (MQ=255)
                                                     tCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATTTTGCCTTTAATTCTTTTTCAGTCATGTCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                             <  1:182759/140‑1 (MQ=255)
                                                     gcGTATCGAATGATGTTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCCCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                             <  2:573794/139‑1 (MQ=255)
                                                       gTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTg                                                                           >  2:449581/1‑140 (MQ=255)
                                                               tgatgGTCCATCTTGTCCTAATTCGAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                   <  2:103826/140‑1 (MQ=255)
                                                                gatgGTCCATCTGCGCCTAAGTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATTTTTCATCAACAATATTAACTGCTTTTTGAATTtaact                                                                  >  2:417353/1‑138 (MQ=255)
                                                                   ggTCCATCTGCTCTTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAt                                                               <  2:162079/140‑1 (MQ=255)
                                                                   ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAt                                                               <  1:284157/140‑1 (MQ=255)
                                                                       cATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATGTTCTCCTAACTTTTTTTGCATTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCg                                                           >  1:320027/1‑140 (MQ=255)
                                                                       cATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCg                                                           >  1:1669/1‑140 (MQ=255)
                                                                            gCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGc                                                      >  1:500945/1‑140 (MQ=255)
                                                                               ccTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTGATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATc                                                   <  2:114622/140‑1 (MQ=255)
                                                                                 tcaTTCTAGCATTTTGCTTTATAATCTTGCCTTTAATTCTTTTTCAGGCATGCCTCCTTTTACAACAGTGAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                 <  2:292727/138‑1 (MQ=255)
                                                                                  aaTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                >  1:583133/1‑140 (MQ=255)
                                                                                    ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTCACAACAGTGAAAATATCTTCCTATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAATCCACATTCGTCAGCATCTTTGa                                              <  2:335441/140‑1 (MQ=255)
                                                                                       tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                           <  1:275777/140‑1 (MQ=255)
                                                                                         gCATTTTGCTTTCTTATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                         <  2:459406/140‑1 (MQ=255)
                                                                                         gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                         <  1:304427/140‑1 (MQ=255)
                                                                                              ttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAt                                    <  1:420011/140‑1 (MQ=255)
                                                                                               tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTCTGACGCCTCTaaat                                   <  2:585786/140‑3 (MQ=255)
                                                                                                 cTTTCTACTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGATTTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtttt                                 <  2:132622/140‑1 (MQ=255)
                                                                                                  tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTACAATCTATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAACCAATTTCGTCAGCATCTTTGACGTCTATAATTTTa                                >  1:310472/1‑140 (MQ=255)
                                                                                                  tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTACAGTAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                                >  2:531980/1‑140 (MQ=255)
                                                                                                  tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGCTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                                >  2:474102/1‑140 (MQ=255)
                                                                                                   ttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                               <  1:229020/140‑1 (MQ=255)
                                                                                                     cTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTTATGCGGTGCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCt                             <  1:654537/140‑1 (MQ=255)
                                                                                                      ttatatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTATACAACAGGTAAAATATATTCATATGCTTCATCAACAATATTAGCTGGTTTTTTAATTAAAGCAATTTCGTCAGCATCTTTTACGTCTCTAATTTTATCTa                            <  2:260256/138‑1 (MQ=255)
                                                                                                             gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACTATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGGCGTCTCTAATTTTATCTACAGTAtt                     >  2:395716/1‑140 (MQ=255)
                                                                                                               cTTTAATTCTTTTTCAGTCATGCCTCCTTTTCCAACAGTTAAAATATATTCATATGTTTCATCCACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                   >  2:305258/1‑140 (MQ=255)
                                                                                                               cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                   <  1:83764/140‑1 (MQ=255)
                                                                                                                   aaTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAt               <  2:268557/140‑1 (MQ=255)
                                                                                                                             cAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAAt     >  2:187993/1‑140 (MQ=255)
                                                                                                                              aGTCATGCCTCCTTTTACAACAGTTGAAATATATTCATATGTTTCATCAACAATATTTGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAACTTTGACATCTCTAATTTTATTTACAATATTAGAAATGATTGTTaagt    >  2:363707/1‑138 (MQ=255)
                                                                                                                              aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATAAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATTTACAGTATTAGAAATGCTTATTAATg    >  1:201619/1‑140 (MQ=255)
                                                                                                                               gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa   <  1:418856/140‑1 (MQ=255)
                                                                                                                                tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat  >  2:527388/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                   
TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAT  >  CP000730/1646053‑1646320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑