Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,876 C→T 55.0% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8760CT55.0% 10.6 / 111.1 100intergenic (+107/+1)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (15/30);  new base T (21/34);  total (36/64)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAT  >  CP000730/35737‑36013
                                                                                                                                           |                                                                                                                                         
gCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAAAAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                                           >  1:518698/1‑140 (MQ=255)
   aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTa                                                                                                                                        >  1:349692/1‑140 (MQ=255)
     tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAAc                                                                                                                                      >  2:635511/1‑140 (MQ=255)
      cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                                                                                     <  2:307882/140‑1 (MQ=255)
            aacgTTTTTGGCGAAAAATGGTTACTTGAACAAGAGCTAAGATAAAATTGTGATCTAATAAAAATAGAATGTTCTGTTGCAAAGTAAACACATCTAGCTAACCACCAATTTATCACGGCATTGTTCGCTTAATTGGCTAg                                                                                                                               <  2:557235/137‑1 (MQ=255)
                      gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCACGATGCTa                                                                                                                     >  1:336889/1‑140 (MQ=255)
                       cGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                                                                                    >  2:84775/1‑140 (MQ=255)
                           aaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAAtttg                                                                                                                >  1:213423/1‑139 (MQ=255)
                               gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTgg                                                                                                            >  2:169961/1‑140 (MQ=255)
                                  tACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATTGAAGGTTCTGTTGCTTAGTATATTAATATAGCTAGCCACTGATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCAt                                                                                                         <  1:280684/140‑1 (MQ=21)
                                     ttGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGCCAGTGTTCGCTTAACTTGCTAGCATGACGCTAATTTCGTGGCATGGc                                                                                                      <  2:334978/140‑1 (MQ=255)
                                          cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaaa                                                                                                 <  2:51706/140‑1 (MQ=255)
                                                cTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGATATGTTTGTGGCATGGCGAAAATCCGTa                                                                                           <  2:306120/140‑1 (MQ=255)
                                                cTAAAAAAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTa                                                                                           <  2:216147/140‑1 (MQ=255)
                                                 taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAg                                                                                          >  1:663515/1‑140 (MQ=255)
                                                     acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTACAAAATCTAGCTAATCACTAATATATCATACCAATATTCGTTTAACTGGTGAGCATGATGCTAATTTCGTGGCATGGCGAAAATGCGTAGATCTGa                                                                                    >  1:288880/15‑140 (MQ=2)
                                                     acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGa                                                                                    >  1:20869/15‑140 (MQ=11)
                                                      caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                   <  1:265277/127‑1 (MQ=11)
                                                       aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCTGTGTTTGTTTATCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                  <  1:194225/128‑1 (MQ=11)
                                                       aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                  <  1:628378/128‑1 (MQ=11)
                                                        aaagtgtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCTCTAATATATCTTGTCAGTGTTCGTTTATCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                 <  1:74933/129‑1 (MQ=11)
                                                        aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                 <  1:517866/129‑1 (MQ=11)
                                                        aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                 >  2:159932/12‑140 (MQ=11)
                                                         aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                                                <  1:534692/130‑1 (MQ=11)
                                                          atTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                 >  2:584782/1‑140 (MQ=255)
                                                          agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                                               >  2:296117/10‑140 (MQ=11)
                                                           gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                              <  1:83130/132‑1 (MQ=11)
                                                           gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                              <  1:123561/132‑1 (MQ=11)
                                                            agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                             <  1:237830/133‑1 (MQ=11)
                                                            agtattttaataaAAATGATGGCTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                             <  2:294229/133‑1 (MQ=11)
                                                              tgactatataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATACCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                             <  2:307935/133‑1 (MQ=255)
                                                              tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                             <  1:504728/140‑1 (MQ=255)
                                                              tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                             <  2:630285/140‑1 (MQ=255)
                                                              tgATCTAATAAAAATAGAAGGTTCTGTTACAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                             <  1:384811/140‑1 (MQ=255)
                                                              tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGCAgagagttg                                                                           <  2:576400/135‑5 (MQ=11)
                                                              tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                           <  2:357206/135‑1 (MQ=11)
                                                              tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                           >  1:545401/6‑140 (MQ=11)
                                                              tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTg                                                                           <  1:34158/135‑1 (MQ=11)
                                                              tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGATAGCATGCTGCTCAGTTCGTGGCATGGCGATAATCCGTAAATCTGCAGAGAcacg                                                                           >  2:329856/6‑137 (MQ=11)
                                                               gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTATATCTGAAGAGACCt                                                                            <  1:356554/140‑1 (MQ=255)
                                                               attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                          <  1:158799/136‑1 (MQ=11)
                                                               attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                          >  1:526189/5‑140 (MQ=11)
                                                               attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGACAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCTGTAGATCTGAAGAGAACTGc                                                                          >  1:118936/5‑140 (MQ=11)
                                                               attttaataaAAAGGATGGCTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATAGATCATGTCAGTGTTCGTTTAACTTGCTAGCATGACGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                          <  2:32631/136‑1 (MQ=11)
                                                                ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTACTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                         >  1:46612/4‑140 (MQ=11)
                                                                ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                         <  1:665108/137‑1 (MQ=11)
                                                                ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                         <  2:14986/137‑1 (MQ=11)
                                                                  ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTTATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                       <  1:595201/139‑1 (MQ=11)
                                                                  cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                         <  1:635511/140‑1 (MQ=255)
                                                                   taataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTCGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                        <  2:354634/140‑1 (MQ=255)
                                                                   taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                      <  2:45730/140‑1 (MQ=11)
                                                                    aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAATATTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                     >  1:228945/1‑140 (MQ=11)
                                                                     ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                    <  1:602248/140‑1 (MQ=11)
                                                                           atggctgggtCTGTGGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTta                                                               <  2:33711/131‑1 (MQ=1)
                                                                             tgctttttatGTTGCAAAGTAAAAAAATATAGCGTATCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGTCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                                              <  2:454308/131‑1 (MQ=1)
                                                                             tgAAGGTTCTGTTGAAAAGCAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttcgt                                                              <  2:504480/139‑4 (MQ=9)
                                                                                tggttaaatttcAAAGCAAAAAAATATAGATAACCACTAATTTATCATGTCAGTGTTCGCTTAACTGGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                                           <  2:546926/129‑1 (MQ=1)
                                                                                                    aacatagAGGTAACTATTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTTTTATGGCGAAAATTCTTATATTTGAAGTGACCTGCAGTTTTTTTTATATAGAGCGTAAATACATTCAATa                                       <  1:508092/137‑1 (MQ=1)
                                                                                                              tatccACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAg                             <  1:15170/137‑1 (MQ=1)
                                                                                                                    ctaatacaccATGTTAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTATATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCt                       <  1:360623/131‑1 (MQ=1)
                                                                                                                       atatattatGTCAGTGTTCGTTTACCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGATAGTTGCGGTTCTTGTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTg                    <  1:385591/137‑1 (MQ=1)
                                                                                                                       atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTGAATACGTTTTAAAGTATTCTTTg                    >  2:531082/4‑140 (MQ=1)
                                                                                                                       atatATCATGTCAGTGTTCGTATAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGATCGTAAATACATTCAATACCTTTTAAAGTATTCTTTg                    <  1:462310/137‑1 (MQ=1)
                                                                                                                       atatATCAGGTCATTGTTCTTTTAACTTGCAAGCATGATGCTAATTTCCTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATCGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTg                    <  1:320431/137‑1 (MQ=1)
                                                                                                                             caggTCCGTGTTGGCTTAACTTGCTAGCGTGATGCTAATTTCGTGGCATTGCGAAAATCCGTAGATTTGAAGAGACCTGCGGCTCTTTCTATATATAGCGTAAATACCTTCAATACCGTTTAATCTACTCTTTGCTGTAt              <  2:276759/137‑1 (MQ=1)
                                                                                                                                gtgaGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATATCTCCAATACCTTTTAAAGTATTCTTCGCTGCATTGa           <  2:373396/137‑1 (MQ=1)
                                                                                                                                   ggggtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGTTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc        <  2:255617/137‑1 (MQ=11)
                                                                                                                                   cgtgtTCGCTTAACTTGCTAGCATGTTGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATCCACTCAAAGCCTTTTAAAGTATTCTTTGCTTTATTGATAc        <  1:489477/139‑1 (MQ=11)
                                                                                                                                   aGTGTTCGTTTAACTTGGTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc        <  1:231026/140‑1 (MQ=11)
                                                                                                                                   aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc        <  1:571427/140‑1 (MQ=11)
                                                                                                                                   aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc        >  1:18135/1‑140 (MQ=11)
                                                                                                                                   aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTATATTGATAc        >  1:86527/1‑140 (MQ=2)
                                                                                                                                   aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAGATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc        <  1:656331/140‑1 (MQ=2)
                                                                                                                                   aGTGTTCGCTTAACTCGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc        <  1:656236/140‑1 (MQ=2)
                                                                                                                                    gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGAGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt       >  1:284000/1‑140 (MQ=11)
                                                                                                                                    gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGAGAAAATCCGTGAATCTGAAGAGACCTGCGGTTCGCTTTATATAGAACGTAAATACAATCAAACAATCTTTAAATATACTTTGCTGTATTGATAca       >  2:454330/1‑139 (MQ=11)
                                                                                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt      >  1:217655/1‑140 (MQ=255)
                                                                                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt      >  2:478886/1‑140 (MQ=255)
                                                                                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt      <  2:279644/140‑1 (MQ=12)
                                                                                                                                     tgtTCGCTTAACTTGCTAGCCTTTGGCTTATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt      >  2:642331/1‑140 (MQ=11)
                                                                                                                                     tgtTCGCTTAACTTGCTAGCATGCTGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACATTTTAAAGTATTCTTTGCTGTATTGATACtt      >  1:210348/1‑140 (MQ=12)
                                                                                                                                     tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCCTGGCGAAAATGCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTAACAGTATTCTTTGCTGTATTGATACtt      >  2:197614/1‑140 (MQ=11)
                                                                                                                                     tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt      >  1:494598/1‑140 (MQ=17)
                                                                                                                                     tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt      >  1:306929/1‑140 (MQ=17)
                                                                                                                                      gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGTAAAATCCGTAGATCTTAAGAGACCTACGGTTCTTTTTATATAGAGCGGAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt     <  2:649666/140‑1 (MQ=11)
                                                                                                                                      gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt     <  1:645293/140‑1 (MQ=11)
                                                                                                                                      gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt     >  1:108877/1‑140 (MQ=17)
                                                                                                                                      gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt     <  2:548954/140‑1 (MQ=16)
                                                                                                                                      gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAACCGTAGATCTGAAAAGACCTGCGGTACTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt     >  1:358220/1‑140 (MQ=11)
                                                                                                                                      gtTCGCTTAACTTTCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCCGTAGATCTGAAGAGTCCTGTGGTTCTTTTTATATAGAGCGTAAATACACTCAAGACCTTTGAAAGTATTCTTTGCTGTATTGATACttt     <  1:218079/140‑1 (MQ=11)
                                                                                                                                      gtTCGCTTAACTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt     <  1:80714/140‑1 (MQ=16)
                                                                                                                                      gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt     <  1:360350/140‑1 (MQ=18)
                                                                                                                                       ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg    <  2:349688/140‑1 (MQ=16)
                                                                                                                                       ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTGCGGTTCTTTTTATATAGAGCCTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACATTg    >  1:311666/1‑140 (MQ=11)
                                                                                                                                       ttCGCTTAACTTTCTAGCCTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATATAGCATAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg    <  2:57776/140‑1 (MQ=11)
                                                                                                                                        tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa   <  2:628678/140‑1 (MQ=16)
                                                                                                                                        tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa   >  2:391978/1‑140 (MQ=12)
                                                                                                                                        tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa   <  1:270266/140‑1 (MQ=18)
                                                                                                                                         cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt  >  2:349203/1‑140 (MQ=17)
                                                                                                                                         cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt  >  2:546368/1‑140 (MQ=17)
                                                                                                                                           |                                                                                                                                         
GCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAT  >  CP000730/35737‑36013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: