Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,917 C→T 44.8% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,9170CT44.8% 44.9 / 153.6 134intergenic (‑1000/‑168)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/40);  new base T (37/22);  total (71/63)
Fisher's exact test for biased strand distribution p-value = 5.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 137 total aligned reads displayed.

TGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATT  >  CP000730/1929778‑1930056
                                                                                                                                               |                                                                                                                                                  
tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc                                                                                                                                                    <  1:249326/140‑1 (MQ=255)
   cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                                                                                 >  1:407211/1‑140 (MQ=255)
     atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGAACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                                                                              >  1:336112/2‑140 (MQ=255)
         ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                                                                          <  2:340199/140‑4 (MQ=255)
         ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                                                                          <  1:500520/140‑4 (MQ=255)
           gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                                                                        <  1:652921/140‑3 (MQ=255)
                ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                                                                   >  1:378478/7‑140 (MQ=255)
                ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                                                                   >  1:444181/7‑140 (MQ=255)
                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                  >  2:262819/6‑140 (MQ=255)
                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                  >  2:223806/6‑140 (MQ=255)
                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATGACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                  <  2:226637/135‑1 (MQ=255)
                  cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGATGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                 >  1:297137/5‑140 (MQ=255)
                  cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                 >  1:262654/5‑140 (MQ=255)
                  cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                 >  2:250494/5‑140 (MQ=255)
                   acagatagtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                <  2:152124/132‑1 (MQ=255)
                    ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTg                                                                                                                                >  2:156940/1‑140 (MQ=255)
                    cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAAAGGAGGTGCATTACTTTGCTAATCATTTTCGGTAACAACATAGGACCAGTCATCAGTGGCTGTGCTGTTTCGTATTATACTTATTgg                                                                                                                               >  1:337951/3‑140 (MQ=255)
                     agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                              <  2:36775/139‑1 (MQ=255)
                     agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                              <  2:383188/139‑1 (MQ=255)
                      gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                                                                              <  2:113652/140‑1 (MQ=255)
                      gatgatATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTATACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                                                                              <  1:110705/140‑1 (MQ=255)
                         ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                          <  1:426444/138‑4 (MQ=255)
                          ttataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                         <  2:615958/139‑1 (MQ=255)
                          gttaaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                         <  2:332591/136‑1 (MQ=255)
                          gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                         >  1:144792/2‑140 (MQ=255)
                          atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                          <  2:495638/140‑1 (MQ=255)
                          atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                          <  2:658037/140‑1 (MQ=255)
                           tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                        >  2:558378/1‑140 (MQ=255)
                            ttaataGTAGAGCCGCTGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                                                                        >  2:146383/2‑140 (MQ=255)
                            cgatctatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTGACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                       >  2:6591/8‑139 (MQ=255)
                             taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATAATAGCACCAGTTATCAGTGGCTGTGCCAATGCATTTTTTTCTTATTGGCTAAGTAg                                                                                                                       <  1:28062/140‑1 (MQ=255)
                              aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                                     <  1:540592/140‑4 (MQ=255)
                              aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                                     >  1:667840/1‑140 (MQ=255)
                               ataCTAGAGTAGCCTATCTCTCAGGCAAAAATT‑‑GAAGAAGAGAGGAGGTGAATTAATTTGAAAAGCATTTTCAGTTGCATCAGATCACCAGTCATCAGTGGCTGTGGTGTTGCGTATGATACTTATTGGCTTAGTAAACg                                                                                                                    <  2:657606/140‑1 (MQ=255)
                                 agtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTACTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                                                                   <  1:116665/140‑1 (MQ=255)
                                  atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGACGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                                                                 >  2:433154/2‑140 (MQ=255)
                                   tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                                >  2:353430/1‑139 (MQ=255)
                                      agacGCCTATCTCTCAGGCGACAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                             <  1:179096/137‑5 (MQ=255)
                                      agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                             >  1:644172/1‑136 (MQ=255)
                                      agTCGCCTACCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTTATCAGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                              <  1:173036/140‑1 (MQ=255)
                                       gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                            >  1:438656/1‑135 (MQ=255)
                                       gTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGATCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATCCTTATTGGCTTAGTAAACGCaaaaaat                                                                                                            >  2:188322/1‑135 (MQ=255)
                                       gTCGCCTATATCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                            >  2:251746/1‑135 (MQ=255)
                                        tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTTCTCAATTGCCGCCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                           <  1:484475/140‑7 (MQ=255)
                                          gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑aa‑‑aa                                                                                                      <  2:177297/140‑1 (MQ=255)
                                          gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                                                                         >  1:186500/1‑132 (MQ=255)
                                           cctctctttacggCGTAAATTGAGACCCAGAGAGGAGGTGTATAAGGTGTTGGTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGCAATAC‑‑AA‑‑AAt                                                                                                     <  2:179563/129‑1 (MQ=255)
                                           ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AAt                                                                                                     >  1:264990/1‑140 (MQ=255)
                                           ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                        >  1:30862/1‑131 (MQ=255)
                                           ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                        >  2:324772/1‑131 (MQ=255)
                                             tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                      >  1:307337/1‑129 (MQ=255)
                                              aTCTCTCAGTCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACCTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                     <  2:532574/140‑13 (MQ=255)
                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattact                                                                                                     >  2:624595/1‑128 (MQ=255)
                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                     >  1:314945/1‑128 (MQ=255)
                                                     aGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACata                                                                                          >  2:53056/1‑140 (MQ=255)
                                                            aaTTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGc                                                                                 >  2:298503/1‑140 (MQ=255)
                                                             aattgacgcctgagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCa                                                                                <  1:45457/129‑1 (MQ=255)
                                                             aTTTAGGCGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCa                                                                                <  2:117695/140‑1 (MQ=255)
                                                               ttAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTTACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCAcc                                                                              >  2:355254/1‑140 (MQ=255)
                                                                     gCAGATACGAGTTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATCGGCTAAGTCGCCGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATaaa                                                                        <  1:657444/140‑1 (MQ=255)
                                                                       agagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATaaaaa                                                                      <  1:546191/140‑1 (MQ=255)
                                                                        gagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGAACCAATAAAAAt                                                                     >  1:373733/1‑140 (MQ=255)
                                                                         agagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATc                                                                    >  1:442154/1‑140 (MQ=255)
                                                                         agagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATc                                                                    <  1:581282/140‑1 (MQ=255)
                                                                           gggaggGGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCCAAAAAAATcac                                                                  >  2:541456/2‑138 (MQ=255)
                                                                            ggaggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATcccc                                                                 <  1:420496/140‑1 (MQ=255)
                                                                             gaggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                                                                <  2:375683/140‑1 (MQ=255)
                                                                               ggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCCTTGCGTTTTTTTCTTATTGGCTAAGTAGAGGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCACTAAAAATCCCCCCa                                                              >  2:293383/1‑140 (MQ=255)
                                                                                gtgtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                                                             >  1:556422/1‑140 (MQ=255)
                                                                                  gtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa                                                           >  1:634105/1‑140 (MQ=255)
                                                                                  gtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGAAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCACCTCACTa                                                           >  1:433186/1‑140 (MQ=255)
                                                                                       aaGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGc                                                       <  1:359776/140‑1 (MQ=255)
                                                                                        aGGTGATGCTTATTTTCTTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑CCCCGGTCACAGATAGCCGCACCAATAAAAATCCCCTCACTACCGCa                                                      <  2:607591/140‑1 (MQ=255)
                                                                                            gTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa                                                    >  2:255216/1‑140 (MQ=255)
                                                                                            gTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa                                                    >  1:17989/1‑140 (MQ=255)
                                                                                              tgttGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTatttgcggt                                                   <  1:601680/137‑9 (MQ=255)
                                                                                               cacggctATTTTCGTTCACATCATAGCGCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATa                                                  <  2:666234/134‑1 (MQ=255)
                                                                                                   cTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGa                                              >  1:60442/1‑140 (MQ=255)
                                                                                                    ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGCCATATAGCCGCACCAATACACAACCCCTCACTACCGCAAGTaacgcg                                             >  2:552250/1‑135 (MQ=255)
                                                                                                    ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATCGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATTAAAATCCCCTCACTACCACAAagagagag                                             >  2:424809/1‑140 (MQ=255)
                                                                                                     tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                            <  2:58478/140‑1 (MQ=255)
                                                                                                     tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGGTTTGTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                            >  1:457144/1‑140 (MQ=255)
                                                                                                       ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                          <  1:290266/140‑1 (MQ=255)
                                                                                                       ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                          <  1:130121/140‑1 (MQ=255)
                                                                                                                   tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCCAAAAGTGAGGGGATTGGTGTATaa                              <  2:643649/140‑1 (MQ=255)
                                                                                                                   tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGAATaa                              >  2:665917/1‑140 (MQ=255)
                                                                                                                    catAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAg                             <  1:146383/140‑1 (MQ=255)
                                                                                                                      tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGAAAAAATCCCCACACTACTGCAATAGTGTGGGGATTGgtgcat                                <  1:102347/140‑4 (MQ=255)
                                                                                                                         cACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCAACAATAAAAATCCCCTCACTACCGCAAATAATGAGGGGATTGGTGTATAAGTAAAt                        >  1:196179/1‑140 (MQ=255)
                                                                                                                                catcaGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGATATATAGCCGCACCAATAAAAATCCCCTCACTAGCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt                 <  1:53056/140‑1 (MQ=255)
                                                                                                                                 atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                <  1:240119/140‑1 (MQ=255)
                                                                                                                                 atcaGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTATCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                <  1:343325/140‑1 (MQ=255)
                                                                                                                                   caGTGGCTGTGCCATTGCGTTTTTTTTTTGTTGGGTGGGAAGACGCAATAC‑‑AA‑‑AAGAGGAGACGTATAGCCGCACCAATGAAAATCCCCTCACTACCGCAAATAGTAGGGGGATTGGTGTATAAGGAAATACTTTTTTTc              >  2:341920/1‑140 (MQ=255)
                                                                                                                                   caGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑CA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTc              >  1:221683/1‑140 (MQ=255)
                                                                                                                                          tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCt       >  2:374244/1‑140 (MQ=255)
                                                                                                                                          tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATACGGAGGGGGTAGGTGTATAAGTAAATACTTTTTTTTGTTTTCt       >  2:370638/1‑140 (MQ=255)
                                                                                                                                            tgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAACACTTATTTTCGTTGTCTTa     <  1:371126/140‑1 (MQ=255)
                                                                                                                                             gCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTaa    <  1:637807/140‑1 (MQ=255)
                                                                                                                                               cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGAGTATAAGTAAATACTTATTTTCGTTGTCTTAAtt  >  1:611717/1‑140 (MQ=255)
                                                                                                                                               cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAAAAAAAAACCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAAACTTATTTTCGTTGTCTTAAtt  >  1:596654/1‑140 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATT  >  CP000730/1929778‑1930056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: