Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,485,771	A→C	8.1%	intergenic (‑32/+292)	mqo ← / ← USA300HOU_2349	malate dehydrogenase (acceptor)/LctP family L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,485,771	0	A	C	8.1%	164.9 / 5.2	66	intergenic (‑32/+292)	mqo/USA300HOU_2349	malate dehydrogenase (acceptor)/LctP family L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base A (26/34);  new base C (3/3);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ATACCGCCACCAATTAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACAAAACCACAAACCTTAACAGCTATCAACCAATT  >  CP000730/2485633‑2485909                                                                                                                                            |                                                                                                                                            ataCCGCCACCAATTAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑Caa                                                                                                                                            <  1:147770/140‑1 (MQ=255)       ccaccaATTAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CCATAGGtt                                                                                                                                      >  1:78917/1‑140 (MQ=255)       ccaccaATTAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CCATAGGtt                                                                                                                                      >  1:573247/1‑140 (MQ=255)         accaATTAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTcc                                                                                                                                    <  1:625603/140‑1 (MQ=255)             aTTAAGTTGACACCTGTTTTGCTTTATTGTTCTGTCATAACAACTACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATATGTTCCCATg                                                                                                                                <  2:603869/140‑1 (MQ=255)             aTTAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACGAATACAGTCACCATATTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATAACACTTTAAATCAATAAGTGTAT‑CAATAGGTTCCAATg                                                                                                                                >  1:62995/1‑140 (MQ=255)              ttAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATgg                                                                                                                               <  1:472898/140‑1 (MQ=255)              ttAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATgg                                                                                                                               <  1:215004/140‑1 (MQ=255)               tAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACTAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGt                                                                                                                              <  1:620179/140‑1 (MQ=255)               tAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGt                                                                                                                              <  1:362097/140‑1 (MQ=255)               tAAGATGACATCTGTTTTGCTATGTTGTGATGTCATAACAAATACAGTCTCACTTTTTAAAATTAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAAGAGGTTCCCATGGt                                                                                                                              <  2:435407/140‑1 (MQ=255)                aaGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTg                                                                                                                             <  2:545988/140‑1 (MQ=255)                  gATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑At                                                                                                                          <  2:455488/140‑1 (MQ=255)                    tGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGc                                                                                                                        <  1:5142/140‑1 (MQ=255)                        aTCTGTTTTGTTATGTTGTCTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTGTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTa                                                                                                                    <  1:314502/140‑1 (MQ=255)                         tCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTAt                                                                                                                   <  2:531043/140‑1 (MQ=255)                          cTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAATTTAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTAtt                                                                                                                  <  1:352125/140‑1 (MQ=255)                             ttttGCTATGTTGTGTTGTCATAACAACTACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAACTCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACt                                                                                                               <  1:377524/140‑1 (MQ=255)                               ttGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTAt                                                                                                             <  1:503175/140‑1 (MQ=255)                                tGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATTAATAATGTAACGTTCTATAAATACTACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATCACTATc                                                                                                            <  1:358092/140‑1 (MQ=255)                                tGCTATGTCGTTTTGTTATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATc                                                                                                            <  2:430600/140‑1 (MQ=255)                                       ttttgttatCATAAAAAATACAGTCCACCTTTTTAAAATGAATAATGGAACGTTTGAGAAATATGACATATTAATACAACTTTAAACACATAAGTGTCT‑CAATAGATTTCCATGGTG‑ATGCTCTATTAATATTAACTATg                                                                                                     >  2:158253/4‑140 (MQ=255)                                          tgttgtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGATATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGtat                                                                                                  <  1:639350/140‑1 (MQ=255)                                          agttgtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGATAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCAATGGTG‑ATGCTCTATTCATATCTACGATGtat                                                                                                  >  1:592715/2‑140 (MQ=255)                                           gttgtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGtata                                                                                                 >  2:352439/1‑140 (MQ=255)                                           gttgtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACCATGaata                                                                                                 >  2:326791/1‑140 (MQ=255)                                           gttgtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACAAATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATAGTG‑ATGCTCTATTACTTACTACTATGtata                                                                                                 >  1:283863/1‑140 (MQ=255)                                            ttgtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTatat                                                                                                >  1:545365/1‑140 (MQ=255)                                            cggtCATAACAAATAGAGTCCCTCTTTTTAAAATGAATAATGGCACGTTCGAGAAATATGACATATTAATACCGCATTAAATCATTAAGTGTCT‑CATGAGGTTCGCGTGGGG‑ATGCTTTATTCATATCTACGATGgatag                                                                                                >  2:7487/3‑139 (MQ=255)                                              gtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CCATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTa                                                                                              <  2:78917/140‑1 (MQ=255)                                              gtCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CCATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTa                                                                                              <  2:573247/140‑1 (MQ=255)                                                 aTAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGTAATACGACATATTAATTTCTTTTTTTTTTTTTTTGTGTTT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAata                                                                                           <  1:39054/140‑1 (MQ=255)                                                   aaCAATAACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATAtt                                                                                         <  2:27577/140‑1 (MQ=255)                                                        aTACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTtata                                                                                    >  2:230043/1‑140 (MQ=255)                                                        aTACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTtata                                                                                    <  1:584258/140‑1 (MQ=255)                                                         tACAGTCCCCCTTTTTAAAATTAATAATCGAACGTTCGAGAAATTCGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CCATCGGTTCCCATGTTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTataa                                                                                   <  2:317096/140‑1 (MQ=255)                                                           cAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTTACT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTAATATCTAATATGTATATTAATATTTTataata                                                                                 >  1:41185/1‑140 (MQ=255)                                                                  ccTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCCCTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAac                                                                          <  1:550992/140‑1 (MQ=255)                                                                    tgttagcactGAATAACGAAACGTTCTAGAAATTCGACATATTAATTCCCCTTTAAATCACTCAGTGTCT‑TAAGAGGGTCCCATGGTC‑ATGCTCTATTACTATCTACAATGTATATTAATATTTTATAATAACGattcca                                                                        <  2:408815/131‑6 (MQ=255)                                                                      tttAATATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAactact                                                                      <  2:11045/140‑1 (MQ=255)                                                                         aaaaTGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTCCTTTa                                                                   >  1:571810/1‑140 (MQ=255)                                                                         aaaaTGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTa                                                                   >  2:500170/1‑140 (MQ=255)                                                                         aaaaTGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTA‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTa                                                                   >  1:275900/1‑140 (MQ=255)                                                                          aaatttttatttgAACGTTCGAGAAATACGAGATATTAATACCAGTTTAAATAAATAAGTCTCT‑CAATCGGTTCCAATGGTG‑TTGCTCTATTACTATTTACTTTGTATATTAATATTTTATAATAATAAAACTACTTTaa                                                                  >  2:37963/13‑140 (MQ=255)                                                                          aaaTGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTaa                                                                  >  2:38323/1‑140 (MQ=255)                                                                           aaTGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAAt                                                                 >  1:272854/1‑140 (MQ=255)                                                                             tGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTCAATATTTGAG‑ATCTCCAATGGTTATATATTAAG‑ATACTCTAATACGATTAACTAGTTATATTAATAGTTTATAATAACGAAACTACTTTAATta                                                               >  1:319434/1‑140 (MQ=255)                                                                                    tGGAACCTTCGAGAAATACGACCTATTAAGACCACTTTAAATCAATAACTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGACACTACTTTAATTATATACtt                                                        <  2:409264/140‑1 (MQ=255)                                                                                     ggAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTAT‑CCATAGGATCTCCTGATG‑TCGCTCTTTCACTATCTCCTATCGAAAATATTCCTTTCAAATAACTAATCTACATGAATcatatttttt                                                       >  2:386534/1‑135 (MQ=255)                                                                                         cGTTCGAGAAATTCGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTAAAAAAACGAAACTACTTTAATTATATACTTTAtgt                                                   <  1:17690/140‑1 (MQ=255)                                                                                              gagaAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAGTTATATACTTTATGTGCgtt                                              >  2:587297/1‑138 (MQ=255)                                                                                              gagaAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTAATTTAATTATATACTTTATGTGCGAt                                              >  2:362036/1‑140 (MQ=255)                                                                                               agaAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATa                                             <  1:628553/140‑1 (MQ=255)                                                                                                 aaaTACGATATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCTATGGTT‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGc                                           <  2:590184/140‑1 (MQ=255)                                                                                                    tACGCCATATTAATATCACTTTAAATCAATAAGTGTTT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATTTACTTTTTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGcaca                                        <  1:226716/140‑1 (MQ=255)                                                                                                    tACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGG‑TCCAAGTTTGAACTCCTTCTTAAGATTAACTATGTATATTAATCTGGTCTAAGAACAAAACTACTATAATTATATACTTTATGTGCAATAGcaca                                        >  1:656804/1‑140 (MQ=255)                                                                                                            atTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACaaa                                <  1:38323/140‑1 (MQ=255)                                                                                                            atTAATACCACTTTAAATCAATAAGTATCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACaaa                                <  1:37963/140‑1 (MQ=255)                                                                                                                 tACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACCAGGACAAAACcac                           >  2:382211/1‑140 (MQ=255)                                                                                                                      cTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGAACAAAGACAAAACCACAAAcc                      >  1:665197/1‑140 (MQ=255)                                                                                                                             tCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATTCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACAAAACCACAAACCTTAACAg               <  1:87584/140‑1 (MQ=255)                                                                                                                              cAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACAAAACCACAAACCTTCACCGc              >  2:322911/1‑140 (MQ=255)                                                                                                                              cAATAAGTGTCC‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAACTATATACTTTATGTGCGATAGCACATAGTTAATTCTTCAAACCTTAACAGc              <  2:473680/140‑1 (MQ=255)                                                                                                                                       tct‑cAATAGGTTCCCATGGTG‑ATGCTCTATTACTATATACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACAAAACCACGAACCTTAACAGCTATCAACCa     >  1:461779/1‑140 (MQ=255)                                                                                                                                         t‑cAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATAGTTTATGTACGATAGCACAAAGACAAAACCACAAACCTTAACAGCTATCAACCaat   >  1:492299/1‑140 (MQ=255)                                                                                                                                           cAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACAAAACCACAAACCTTAACAGCTATCAACCaatt  <  1:278006/140‑1 (MQ=255)                                                                                                                                           cAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAAGAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACAAAACCACAAACCTTAGAAGCTATCAACCagtt  >  2:447432/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                            ATACCGCCACCAATTAAGATGACATCTGTTTTGCTATGTTGTGTTGTCATAACAAATACAGTCCCCCTTTTTAAAATGAATAATGGAACGTTCGAGAAATACGACATATTAATACCACTTTAAATCAATAAGTGTCT‑CAATAGGTTCCCATGGTG‑ATGCTCTATTACTATCTACTATGTATATTAATATTTTATAATAACGAAACTACTTTAATTATATACTTTATGTGCGATAGCACAAAGACAAAACCACAAACCTTAACAGCTATCAACCAATT  >  CP000730/2485633‑2485909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑