| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,298,108 | T→A | 100% | intergenic (+4/‑232) | pnp → / → USA300HOU_1207 | polyribonucleotide nucleotidyltransferase/beta‑lactamase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,298,108 | 0 | T | A | 100.0% | 67.4 / NA | 24 | intergenic (+4/‑232) | pnp/USA300HOU_1207 | polyribonucleotide nucleotidyltransferase/beta‑lactamase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (8/16); total (8/16) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCTGTA > CP000730/1297972‑1298244 | ttCACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATaaa < 1:350241/141‑1 (MQ=255) cacaAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGt > 2:187668/1‑141 (MQ=255) aaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACGCAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACa > 1:208982/1‑141 (MQ=255) aaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACa < 2:113378/141‑1 (MQ=255) gAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCa > 1:244758/1‑141 (MQ=255) ggAAGATGTATTAAAAATCGGGTACACAATTTAAGTTAAGATTACTGAAATTCCT‑GTCTCGTTACATGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGAttt < 1:188164/141‑1 (MQ=255) tATTAAGAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAGGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGc < 1:113925/141‑1 (MQ=255) ggtggtacaATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACg < 1:3830/135‑1 (MQ=255) ggTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATATAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACg < 1:265424/141‑1 (MQ=255) tGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCCCATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGaa > 1:3581/1‑141 (MQ=255) tGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGaa < 2:16576/141‑1 (MQ=255) gAAGTTAAGATTACGGAAATTGAAAAACAAGGT‑CGAGTAAATGCTTCACATCCGGTGTTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtt < 1:187668/141‑1 (MQ=255) aGTTAAGAATACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtttt < 1:615/141‑1 (MQ=255) gATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTTTTTTATTTTTTACGAAGTAACTCGTTTTGACttt > 2:136441/1‑141 (MQ=255) tagaTTGATAACAAAGGG‑CGGGTAAATGCTACGCAGAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTAt < 2:20703/138‑1 (MQ=255) gAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGCGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTAt < 2:239998/141‑1 (MQ=255) aCAAGGT‑CTAGTAAATGCTTCACAAAGAGCATTAGAAGAAAAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTa < 1:19900/141‑1 (MQ=255) aCAAGGT‑CGAGTAAAAGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTa < 2:66136/141‑1 (MQ=255) ggT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAAc > 1:335557/1‑141 (MQ=255) aGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCttt < 1:48384/141‑1 (MQ=255) gTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTtta < 2:3581/141‑1 (MQ=255) cacaTAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTtgtg < 1:346691/141‑1 (MQ=255) agagCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGCATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTCTGTGTGCaa > 2:350428/1‑141 (MQ=255) tAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTaa > 2:368896/1‑141 (MQ=255) atatatAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCtgtt > 2:228566/1‑140 (MQ=255) | TTCACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGT‑CGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCTGTA > CP000730/1297972‑1298244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |