Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 253.4 / NA 76I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (34/42);  total (34/42)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416322‑1416594
                                                                                                                                        |                                                                                                                                         
tttAACTCAATCATAGTAATCCCTCGTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTa                                                                                                                                       <  1:415596/141‑1 (MQ=255)
    aCTCAATCATAGTAATCCCTCTTTAAGTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                                                                   >  2:637159/1‑141 (MQ=255)
     cTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAc                                                                                                                                  <  2:462424/141‑1 (MQ=255)
     cTCAAGCATAGTAATCCCTCTTTAATTGTGTTTTATATTTAATTAGAATTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAc                                                                                                                                  <  2:964903/141‑1 (MQ=255)
       cAATCATAGTAATGCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCCATGATTTCACCTTCTTTTAAAACATAATTGTACTgcaat                                                                                                                                >  2:539220/1‑137 (MQ=255)
       cAATCATAGTAACACCTCTGTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTGCTTTTAAAACTTAATTTTACTTACaa                                                                                                                                >  2:301908/1‑141 (MQ=255)
             tAGTAATCCCTCTTTATTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGAGTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                          <  2:786668/141‑1 (MQ=255)
             tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                          <  2:885950/141‑1 (MQ=255)
             aaGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                          <  2:18728/140‑1 (MQ=255)
               gTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAGTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATCTTTCaa                                                                                                                        >  1:277292/1‑141 (MQ=255)
                       tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAc                                                                                                                >  1:520544/1‑141 (MQ=255)
                       tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAc                                                                                                                <  2:548301/141‑1 (MQ=255)
                       tctGTAATTGTGTGCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAc                                                                                                                <  2:826692/141‑1 (MQ=255)
                        ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACt                                                                                                               >  1:870944/1‑141 (MQ=255)
                           tAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCACTACATCAATTaa                                                                                                            >  1:712447/1‑141 (MQ=255)
                             aTTGGGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                          <  2:367597/141‑1 (MQ=255)
                              ttGTGTTCTATATTTAATTCGACGTTCAGTATACGGATCCACATTCTCATACTTGGAATGATTAATGTTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTACAGtt                                                                                                         >  2:733029/1‑141 (MQ=255)
                              ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                         <  2:649438/141‑1 (MQ=255)
                              ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                         <  2:84439/141‑1 (MQ=255)
                              ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                         <  1:1026224/141‑1 (MQ=255)
                              ttGTGTTCTATATTTAATTAGAAGTTCAGTATACGGATGAAAATGATCATAATTGAAATAATTAATATTAACTCGTTCCATGATATGAACTTAATTTCAAGAGTAATTGTACTGACCATATTTCACTACATGGCTTCAGtt                                                                                                         >  2:114231/1‑141 (MQ=255)
                                 tgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                      <  2:1027530/141‑1 (MQ=255)
                                    tCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgt                                                                                                   <  1:666976/141‑1 (MQ=255)
                                    tCTATATTTAATTACACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATAGTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAAAATCTCTCTTTTGATGACTTAAGTTAtgtgt                                                                                                   <  1:514828/141‑1 (MQ=255)
                                     cTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgtg                                                                                                  >  2:452351/1‑141 (MQ=255)
                                         atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                              >  2:443290/1‑141 (MQ=255)
                                           ttAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataata                                                                                            <  1:468524/141‑1 (MQ=255)
                                            tAATTAGACCTTTAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCCATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                           <  2:28620/141‑1 (MQ=255)
                                              aTTAGACGTTCAGTATCCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaat                                                                                         <  1:871528/141‑1 (MQ=255)
                                              aTTAGACGTTCAGTATACGGATGCAAATGCTCCTACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTATTGACAATATTTCAATACATGACTTATGTTATGTGTGAtaattaat                                                                                         >  1:39860/1‑141 (MQ=255)
                                              aTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTATTGACAATATTTCCATACATGACTTAAGTTATGTGTGAtaataact                                                                                         >  1:165118/1‑139 (MQ=255)
                                              aTTAGACGTTCAGTATACGGATGCAAACGCTCATACTTCAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGGTATGTGTGATaataaat                                                                                         <  2:449033/141‑1 (MQ=255)
                                                ttatggTTCAGTATACGGTTACAAATGCTCATTCTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTATTAAAAAATAATTGTTCTGACATTATTTCAATACATGACTTAAGTTATGTGTGATAataaataa                                                                                       <  2:830282/136‑1 (MQ=255)
                                                tAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACACGACTTAAGTTATGTGTGATAataaataa                                                                                       <  1:673988/141‑1 (MQ=255)
                                                   aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATACTAAATCATGt                                                                                   >  2:677011/1‑141 (MQ=255)
                                                      ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                <  2:847901/141‑1 (MQ=255)
                                                       ttcgTATACAGATGCACATGCTCATTCTTAAAATGATTACACTTACCTCGTCCCATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTACAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                               <  1:126076/138‑1 (MQ=255)
                                                       tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTgc                                                                               >  2:731555/1‑140 (MQ=255)
                                                       tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                               <  2:217434/141‑1 (MQ=255)
                                                        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                              <  1:732100/141‑1 (MQ=255)
                                                        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                              <  1:420736/141‑1 (MQ=255)
                                                          atataCGGATGCAAATGCTTATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                            <  1:1006165/140‑1 (MQ=255)
                                                            ataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATg                                                                          <  1:607291/141‑1 (MQ=255)
                                                                       aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTGAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                                                               <  1:637159/141‑1 (MQ=255)
                                                                       aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                                                               >  1:668381/1‑141 (MQ=255)
                                                                         tGCTTATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTCTGATAATAAATAATGTTTGACGATGTTCTAATACAata                                                             <  1:591154/141‑1 (MQ=255)
                                                                         tGCTCATACTTGAAATGATTTATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                             <  1:928715/141‑1 (MQ=255)
                                                                         ggCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                             <  1:208620/140‑1 (MQ=255)
                                                                         cgCTTATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                             <  1:6406/140‑1 (MQ=255)
                                                                           cTCATACTTGAAATGATTAATATTGCCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAGCATGTTCTAATACAATATg                                                           >  1:676630/1‑141 (MQ=255)
                                                                            tcattcatGAAATGATTAATATTACCTCTGTGAATGATTTTGCATTCTTTTAATTCATAATTGTACTGACAATATTTCATTACGTGACTTAAGTTCTGTGTCATAATAAATAATGTTTGACCATGTTCTagtgtgatgtgc                                                          <  2:834671/141‑12 (MQ=255)
                                                                                 cTTGAAATTATTAATATTACCTCGTTCAATGATTTGACCTTCTTTAAACACATATTTCTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaa                                                     <  1:723081/141‑1 (MQ=255)
                                                                                 cTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaa                                                     <  1:452351/141‑1 (MQ=255)
                                                                                    gAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAATTAATGTTTGACCATGTTCTAATACAATATGCTGtaataa                                                  >  2:923107/1‑141 (MQ=255)
                                                                                    gAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaataa                                                  >  1:265870/1‑141 (MQ=255)
                                                                                     aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATTCTGTGATaaa                                                 >  1:44862/1‑141 (MQ=255)
                                                                                     aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATaaa                                                 >  1:593494/1‑141 (MQ=255)
                                                                                          aTTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACAATGTTCTAATACAATATGCTGTAATAAATCCAt                                            >  2:889947/1‑141 (MQ=255)
                                                                                           ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                           >  2:1027518/1‑141 (MQ=255)
                                                                                           ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                           >  2:74567/1‑141 (MQ=255)
                                                                                           ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                           >  2:997559/1‑141 (MQ=255)
                                                                                             aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATTCTGTAATAAATCCATccc                                         >  2:997797/1‑139 (MQ=255)
                                                                                             aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCat                                         >  2:971190/1‑140 (MQ=255)
                                                                                                   acgtCGTGCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGAtt                                   >  2:1053178/4‑141 (MQ=255)
                                                                                                    ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTa                                  <  1:114084/141‑1 (MQ=255)
                                                                                                    ccTCGTTCAATGATTTGACATTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCCCTTGATTa                                  <  1:677742/141‑1 (MQ=255)
                                                                                                          tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTc                            >  1:229323/1‑141 (MQ=255)
                                                                                                              tGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAg                        >  1:633862/1‑141 (MQ=255)
                                                                                                               gATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGCTTACCGTTCAAAGc                       >  1:158616/1‑141 (MQ=255)
                                                                                                                       cttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAata               >  2:174749/1‑140 (MQ=255)
                                                                                                                                aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATACTAATAATAATGTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      <  1:245449/141‑1 (MQ=255)
                                                                                                                                aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATGTGCTCTGAGAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      <  1:411505/141‑1 (MQ=255)
                                                                                                                                aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      >  2:652291/1‑141 (MQ=255)
                                                                                                                                aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      >  1:342199/1‑141 (MQ=255)
                                                                                                                                   cATAATTGTCTTGACAATATTTCAAGCCATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCTAATGATGCAACTGGtt   <  1:631778/141‑1 (MQ=255)
                                                                                                                                    aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  1:74567/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416322‑1416594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: