| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,719,269:1 | (A)5→6 | 100% | coding (213/705 nt) | USA300HOU_2573 → | dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,719,264 | 1 | . | A | 52.8% | 64.3 / 67.1 | 36 | coding (208/705 nt) | USA300HOU_2573 | dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (8/9); new base A (11/8); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGA > CP000730/2719126‑2719396 | gCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aa > 1:270498/1‑141 (MQ=255) ttGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aa‑aaa > 2:219962/1‑141 (MQ=255) ttACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGCACAACAAGCAACAACGAAATAAGGTCCGCTATAAGCACTGTTAGATATAATAGGTTATGTCTCGATCAATAATGTTTATAACATAATTGCTG‑ca‑aaaacaag > 2:205060/1‑136 (MQ=255) tatataGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGa < 1:218156/141‑1 (MQ=255) tataGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGata < 2:176326/141‑1 (MQ=255) taGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATcc < 2:21705/141‑1 (MQ=255) ggAATGATGTAAGAGGACAACCAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTAAAAGTGACTACTCAGTCAAAAATCCTTATAAACTGATTGATG‑AA‑AACGAAACCCGACTTGATATCCTcgtgagca > 2:192544/1‑134 (MQ=255) ggAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAAc > 1:16744/1‑141 (MQ=255) atgatgTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATg > 1:177070/1‑141 (MQ=255) tgatgTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATg > 2:143661/1‑141 (MQ=255) tgatgTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATg > 2:143692/1‑141 (MQ=255) gatgTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGc < 1:193232/141‑1 (MQ=255) aaaaaataaaaaaaaTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCaa < 2:83453/131‑1 (MQ=255) aaaTTAGGTGTTCATTATGTACAATTAGATGTTATAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCt < 2:119656/141‑1 (MQ=255) aGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCa > 1:216230/1‑141 (MQ=255) ggTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCaaa > 1:76143/1‑141 (MQ=255) gtgtTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAt < 2:108389/141‑1 (MQ=255) tttCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTg < 2:217652/140‑1 (MQ=255) tGTACAATTAGATGTGACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCg < 2:177070/141‑1 (MQ=255) gTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGt > 2:260337/1‑141 (MQ=255) tACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGTAGGTATATCTGGTCAATTTTCAGCACCTCCCAAATTGACACCTCGTg > 2:38385/1‑141 (MQ=255) tACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGCATATCTGGTCAATTTTCAGCACCTTCCAAATTGGCACCTCGTg > 1:136731/1‑141 (MQ=255) tgaGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGTCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCAGCTTCCAAATTGACACCTCGTGACGt < 2:270157/139‑1 (MQ=255) aGATGTTACAAGTGACTACGCAGTCGAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTAGATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTg < 2:94550/141‑1 (MQ=255) aaTGTCACAAGTGACTACTCAGTCAAAAATGCTTATAACATTATTGCCG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaa < 2:11217/140‑1 (MQ=255) aTGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCCATTTTCAGCACCTTCCAAATTGACACCTCGTGCCGTTgaa > 1:194020/1‑141 (MQ=255) tACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGaagaag < 2:227110/141‑1 (MQ=255) tACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGaagaag < 1:46412/141‑1 (MQ=255) aCAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGt > 1:276148/1‑141 (MQ=255) cAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCCGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGtat < 1:276253/141‑1 (MQ=255) aGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGtata > 1:58661/1‑141 (MQ=255) aGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATACTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCCAATTGACACCTCGTGACGTTGAAGAAGtata > 2:51724/1‑141 (MQ=255) actactCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATAACaaa > 1:3577/1‑141 (MQ=255) ctCAGTCAAAAATGCTCATAAGATGATTGCTG‑AAGAAAGAATGGTGTCGTGATATCCTCTTTAACTATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGa < 2:110723/141‑1 (MQ=255) aGTCAAAAATGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACATCGTTACGTTGAAGAAGTATATCAAACGAATg < 2:116125/141‑1 (MQ=255) aaTGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCAAATTCCTATTTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGt > 1:190789/1‑141 (MQ=255) tGCTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTa > 1:137366/1‑141 (MQ=255) cTTATAACATGATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATc > 1:244268/1‑141 (MQ=255) gATTGCTGAAA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAatga > 1:168547/1‑141 (MQ=255) | GCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AA‑AAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGA > CP000730/2719126‑2719396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |