Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,279,7050GA100.0% 86.7 / ‑6.5 34intergenic (+1373/+6659)mtlD/glmMmannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/19);  minor base T (0/1);  total (14/20)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTG  >  CP000730/2279568‑2279840
                                                                                                                                         |                                                                                                                                       
ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGAGGTTACtg                                                                                                                                      >  2:27680/1‑141 (MQ=255)
   tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg                                                                                                                                   <  2:16154/141‑1 (MQ=255)
      agTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATGCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATGAGCTTTCGGTTTATCATTTGCATCATCCGTTGCTGATTTTACTGTTGttt                                                                                                                                >  1:119623/2‑141 (MQ=255)
        ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCa                                                                                                                              >  1:227722/1‑141 (MQ=255)
        ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCa                                                                                                                              >  1:17531/1‑141 (MQ=255)
        ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTATTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCa                                                                                                                              <  1:110927/141‑1 (MQ=255)
        ttattaGTTGATTCTACTTCTGCTTTACTAGTTACATCGTTATCCACTGTCGGACTGTTTGTGGATGCATCTACACTAGAATTGTTATGAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCa                                                                                                                              <  1:59390/141‑1 (MQ=255)
         tattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAc                                                                                                                             <  2:205611/141‑1 (MQ=255)
            taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCAACAGCCACCCATGTTACTGTTGTTTCACCTg                                                                                                                          <  1:64805/141‑1 (MQ=255)
                           cTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                            <  1:215822/124‑1 (MQ=255)
                           cTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                            >  2:215822/1‑124 (MQ=255)
                                  aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTCGTTTCACCTGTTGCCGCATCACTATTATTTgg                                                                                                    <  2:30307/141‑1 (MQ=255)
                                       ttACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACAATTATTTGGtgttg                                                                                               <  2:184069/141‑1 (MQ=255)
                                          cATCGTTATTCTTTGTCTGACTGTTTGTTTAGGCATCTGGACTAGAATGGTTATTAGCTTTCGGTTTATCATTTGTATCATCGGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCGTCACTATTATTTGGTGTTGTCg                                                                                            <  2:132563/141‑1 (MQ=255)
                                          cATCGTTATCCAGTGTCGGACTGTTTGTTGATGAATGTACACTGGAATTGTTTTTAGCTTGCGGTTTATCATTTGCATCAACAGTTGCTGATGTTACGGTTGTTTCACCTGTTGCCGCATCACAGTTATTGGGtgttgttg                                                                                            >  2:247075/1‑139 (MQ=255)
                                              gTTTTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                        <  2:3927/141‑1 (MQ=255)
                                                  tCCATTGTCGGACTGTTTGTTTATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGCTGTTACTGTTGTTTCACCTGTTGCCGCCTCACTATTATTTGGTGTTGTCGCAGAAGCg                                                                                    >  1:154111/1‑141 (MQ=255)
                                                  tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACGATTATTTGGTATTGTCGtagtaacg                                                                                    >  1:154238/1‑136 (MQ=255)
                                                    cATTGTCGGACGGGTGGTTGATGCATCTACACTAGAATTGTGATCAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                                  <  1:222645/141‑1 (MQ=255)
                                                      ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                                >  2:218390/1‑141 (MQ=255)
                                                        ttCGGACTGTTTGTTGTTGCATCTAGACTAGAATTGTTATTGGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTTCTGGTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt                                                                              <  2:50670/140‑1 (MQ=255)
                                                                   tgttgATGACTCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTCCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                   <  1:12885/141‑1 (MQ=255)
                                                                                                        ggTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt                              >  2:160119/1‑141 (MQ=255)
                                                                                                           ttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGGTACGTTAGGTTGTCCAGTATTTTCTGgt                           >  2:159632/1‑141 (MQ=255)
                                                                                                            tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGttg                          >  1:84874/1‑141 (MQ=255)
                                                                                                               cATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTg                       <  1:154613/141‑1 (MQ=255)
                                                                                                                     gatcatcTGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATTCGTTTGGTTGTCCAGTATTTTCTGGTGTTGCATTAg                 <  2:240802/140‑1 (MQ=255)
                                                                                                                           caGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTg           >  2:205458/1‑141 (MQ=255)
                                                                                                                             gTTGCTGATTTTACTGTTTTTTCACCTGTTGCCGCATCACTATTATTTGGTTTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTTTTTTCTGGTGTTGCATTAGCATTTGaa         <  2:154238/141‑1 (MQ=255)
                                                                                                                             gTTGCTGATTTTACTGTTGTTTCACCTGTTGCCGCATCACTATTTTTTGGTGTTGTCGGTGATGCGTCTGCTTTGCCATTTGCTGTCGTCTCAGTTTCGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa         <  2:154111/141‑1 (MQ=255)
                                                                                                                             gTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGATGTTGTCGATGTAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGTTCCGATAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa         >  2:120886/1‑141 (MQ=255)
                                                                                                                                  tGATGTTACTGTTGTTTCACCTGATTCTGCATCACTATTATTTGGTGTTGTCGGAGATTCTTATGCTTTTCTATTATCTGTCTTCTCAGATTCGTTTGCTTGCCCAGTATTTTCTGTTGTTGCATTAGCATTTGAATTTGc    <  2:119623/141‑1 (MQ=255)
                                                                                                                                    ttGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg  <  2:227722/140‑1 (MQ=255)
                                                                                                                                    gtGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATGAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg  <  2:17531/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTG  >  CP000730/2279568‑2279840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: