Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 584,121 G→T 100% intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730584,1210GT100.0% 56.2 / ‑6.5 23intergenic (+205/‑67)rplA/rplJribosomal protein L1/ribosomal protein L10
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (11/11);  total (11/12)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCA  >  CP000730/583983‑584259
                                                                                                                                          |                                                                                                                                          
aGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTg                                                                                                                                          >  1:21227/1‑141 (MQ=255)
    aTTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTa                                                                                                                                      >  2:182405/1‑141 (MQ=255)
                       tATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTTGTTAGAAAGTGCTTTTTttatta                                                                                                                   >  2:70354/1‑141 (MQ=255)
                        aTTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTaa                                                                                                                  <  1:58600/141‑1 (MQ=255)
                         ttGTGATTATTTTACCTAAGCCAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAt                                                                                                                 <  1:102675/141‑1 (MQ=255)
                          tgtgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAtt                                                                                                                >  2:104252/1‑141 (MQ=255)
                                       ccTAAGACAGTAGGTGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAc                                                                                                   <  2:92412/141‑1 (MQ=255)
                                           aGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaaa                                                                                               >  1:69170/1‑141 (MQ=255)
                                              cAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAAGTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAt                                                                                            <  1:83701/141‑1 (MQ=255)
                                                              taACTTAAAAGTTATCCTGCCGAGGCCAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCt                                                                            <  1:57676/141‑1 (MQ=255)
                                                                       aTTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCGTTTTTTATTAATTTTAAATAAAGCACCCACAATTTAAATGGAGGTGGCTGACTGTCTg                                                                   >  2:109699/1‑141 (MQ=255)
                                                                           agccTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTAt                                                               <  1:238519/139‑1 (MQ=255)
                                                                           aTCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTTTTTTTTTTTGAAAGTGCTTTTTTTATTAATTTTAATACAAGCATCAAAAATTTAAATGGTGGTGTTTGTATGttttcttt                                                               >  2:125588/1‑134 (MQ=255)
                                                                                     ggCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCt                                                     <  1:25269/141‑1 (MQ=255)
                                                                                     ggCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGACTGGGGTAGGAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATAATTGAGGCt                                                     >  1:82614/1‑141 (MQ=255)
                                                                                       cTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaa                                                   >  2:231685/1‑141 (MQ=255)
                                                                                             ttGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTGTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaaca                                             <  1:182405/141‑1 (MQ=255)
                                                                                                 agtGAACGTGATGATGTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATAAAAAAAGAAAAAAAACAACTa                                         <  2:208477/139‑1 (MQ=255)
                                                                                                                      tctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGACATTGCTGAGGTa                    >  2:166802/1‑141 (MQ=255)
                                                                                                                         ttCAAGCCATTCTTGCCTTGGGTAGAAAGTGCTGTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTa                 <  2:210763/141‑1 (MQ=255)
                                                                                                                           cAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATc               >  2:170389/1‑141 (MQ=255)
                                                                                                                                aCTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAAtt          <  1:120679/141‑1 (MQ=255)
                                                                                                                                        ccGGGGGTAGAAAGTGCTTCTTTGATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCGGAGGTACTATCAAATTCAGTTTCa  <  1:109699/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCA  >  CP000730/583983‑584259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: