Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 46.8% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA46.8% 48.5 / 184.3 167intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (48/40);  new base A (48/30);  total (96/71)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 169 total aligned reads displayed.

GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAT  >  CP000730/76213‑76491
                                                                                                                                            |                                                                                                                                          
gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                                                                            <  2:22444/141‑1 (MQ=32)
 tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGt                                                                                                                                           <  1:19407/141‑1 (MQ=17)
   tGAACTATTTCAGATCTAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTACGATATGCATTGAGGTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCt                                                                                                                                         >  2:213949/1‑141 (MQ=11)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                                                                                      <  1:97905/141‑1 (MQ=17)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTGCAACATTCAACGGTCTAcc                                                                                                                                      >  1:196248/1‑141 (MQ=255)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                      >  1:60671/1‑141 (MQ=12)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                      >  1:213637/1‑141 (MQ=12)
        tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                                                    <  2:104421/141‑1 (MQ=17)
        tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                                                    <  2:272632/141‑1 (MQ=32)
         ttttCCGATATAAACAATTTCACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                                                   <  2:193461/140‑1 (MQ=11)
         aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                   >  1:356484/1‑141 (MQ=17)
         aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                   >  1:303221/1‑141 (MQ=17)
          tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTGAACGTTCATCATTACACGATCTACCGCTg                                                                                                                                  >  2:41242/1‑141 (MQ=11)
          tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                                                  <  1:112097/141‑1 (MQ=12)
          tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                                                  <  1:92849/141‑1 (MQ=12)
            tgaGATATAAACATTTTAACAAGGTTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTggg                                                                                                                                <  2:289370/139‑1 (MQ=255)
             cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACAATCTACCGTTGGGt                                                                                                                               >  2:139883/1‑141 (MQ=255)
              aGATATAAACAATTTACCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                                                              >  2:171932/1‑141 (MQ=11)
              aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                                                                              >  1:164112/1‑141 (MQ=32)
              aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                                                              <  1:116124/141‑1 (MQ=255)
              aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                                                              >  2:119287/1‑141 (MQ=255)
                atatAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                                                                            <  1:271861/141‑1 (MQ=17)
                  ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                                                          <  1:138836/141‑1 (MQ=17)
                   taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                                                         <  2:305998/141‑1 (MQ=17)
                   taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                                                         >  1:66343/1‑141 (MQ=17)
                   taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATACGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                                                         >  2:27763/1‑141 (MQ=17)
                     aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAat                                                                                                                       <  2:54938/141‑1 (MQ=32)
                      aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                                                                                      <  1:150092/141‑1 (MQ=255)
                      aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                                                                                      >  2:261069/1‑141 (MQ=12)
                        aaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                                                                                    <  1:137732/141‑1 (MQ=17)
                         aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTAAACGATCTCCCGTTGGGTTCAAGAACATGc                                                                                                                   >  2:84667/1‑141 (MQ=11)
                          tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACTATCTACCGTTGGGTTCAAGAATATGcc                                                                                                                  <  2:3467/141‑1 (MQ=255)
                           ttAACAAGGATGTTATCACTGTGACTGTGGGCTACGATCACAGAAACGTGCACTCTGATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                                                                                 <  1:365844/141‑1 (MQ=11)
                           ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                                                 >  2:83311/1‑141 (MQ=255)
                            tAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                                                                                                                <  2:334809/141‑1 (MQ=17)
                            tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                                                                                <  1:130882/141‑1 (MQ=12)
                             aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                                               >  1:281408/1‑141 (MQ=17)
                             aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTAATCCTTCAACGGTCTACCGTTGGGTTCAAGAATGTGCCCCa                                                                                                               >  2:8626/1‑141 (MQ=12)
                             aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                                               <  2:232981/141‑1 (MQ=12)
                              aCAAGGGTGTTCTCACTGTAGCGGTTGGCTACTATCTAAGTTATGCATGGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                              >  2:370948/1‑141 (MQ=11)
                              aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCCGAAATATTAAGGGAACGTGGTGTAAACGTTCAGCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                              >  2:113243/1‑141 (MQ=12)
                              aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCGAAGATATGCTCTGATGTGTCTTGATATATCTGAAATATTAAGGGAACGTGGTGTACACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                              >  2:134410/1‑141 (MQ=11)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAAATTAAGGGAACGTGGTGTAAACGTTCAGCATTAAACGGTCTACGGTTGGTGTCAAGATTATGCCCCaa                                                                                                              >  2:72824/1‑141 (MQ=11)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTGTCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                              >  1:249234/1‑141 (MQ=255)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTATGAGAACGTGGTGTAAAAGTTCATCATTCAACGATCTAACGTTGGGTTCAAGAATGTGCCCCaa                                                                                                              >  1:85380/1‑141 (MQ=11)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                              >  1:98996/1‑141 (MQ=255)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATAATTCAACGATCTACCGTTGGGTTCAAGAATATACCCCaa                                                                                                              >  2:135193/1‑141 (MQ=11)
                                aaGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATGTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTATACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                                                            >  2:27694/1‑141 (MQ=12)
                                aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                                                            >  2:283246/1‑141 (MQ=12)
                                  ggATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                                                                          >  2:199765/1‑141 (MQ=17)
                                    aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                                                        <  1:334333/141‑1 (MQ=255)
                                    aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                                                        <  2:310590/141‑1 (MQ=255)
                                     tGTTATCACTGTAGCTGTTGGTTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                       <  1:152956/141‑1 (MQ=25)
                                       ttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                                                     >  1:336915/1‑141 (MQ=25)
                                        tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                    >  2:235591/1‑141 (MQ=25)
                                        tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCTTGATATATTTGAAATATTAAGAGATCGTGGTGTAAACGTTCATCATTCAACGTTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                    <  2:19584/141‑1 (MQ=11)
                                        tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                    <  1:297940/141‑1 (MQ=255)
                                          tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                                                  >  2:203350/1‑141 (MQ=255)
                                              tGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                                                              <  1:28773/141‑1 (MQ=21)
                                                tagccaacggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                            >  2:98174/9‑141 (MQ=11)
                                                tAGCCGTTGGCTACTATCTAAGATCTGCATTGAGCTATCGTGATATATCGGAAGTATTAAGAGACGGTGGAGAAAAGGTTCATCATTCAACGATCGAGCGTTGGGTGCAAGAATATGCCCCAAGTTTAGATCAAATTTGga                                                                                            >  2:320178/1‑141 (MQ=11)
                                                tAGCCGTTGGCTACTAGCTAAGATATGCATTGAGCTATTGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATACAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                            >  1:207752/1‑141 (MQ=11)
                                                  gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                          >  1:245417/1‑141 (MQ=255)
                                                      ttGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGACTATGCCCCAATTTTATATCAAATTTGGAAGaaaa                                                                                      >  1:68700/1‑141 (MQ=11)
                                                      ttGGATAGTATCAAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGATTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaa                                                                                      >  2:44382/1‑141 (MQ=11)
                                                      gtGGCTAGTAGATAAGATATGAATTGACTTATCGTGATATATCTGAAACATTAAGGGAACGTTGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaa                                                                                      <  1:23716/140‑1 (MQ=11)
                                                        ggttacttTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                    <  2:130687/138‑1 (MQ=255)
                                                        ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                    <  1:197693/141‑1 (MQ=35)
                                                         gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATGTGCTGGAATTTTATAACAACATTGGAAGAAAAAGc                                                                                   >  2:184108/1‑141 (MQ=11)
                                                         gCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                                                   >  1:350167/1‑141 (MQ=255)
                                                          ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                  <  2:24166/141‑1 (MQ=35)
                                                           tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGcgt                                                                                 <  2:343328/141‑3 (MQ=25)
                                                             ctaTCTAAGTTATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                               <  2:199624/141‑1 (MQ=25)
                                                             ctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGTACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                               >  1:246750/1‑141 (MQ=25)
                                                             ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                               <  1:104061/141‑1 (MQ=255)
                                                              aaTCTAAGATATTCATTGAGCTAACGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                              <  2:104296/140‑1 (MQ=11)
                                                                 atAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAGGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                                                           <  2:286866/140‑2 (MQ=255)
                                                                  tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaaa                                                                          >  2:207254/1‑139 (MQ=18)
                                                                   aaGATATGCATGGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaacc                                                                         >  2:100983/1‑138 (MQ=11)
                                                                    aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTCGAAGAAAAACCATaaaaaagct                                                                        >  2:176971/1‑137 (MQ=9)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                        >  1:316034/1‑137 (MQ=11)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaaact                                                                        >  2:69117/1‑137 (MQ=11)
                                                                      atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAACAAGCATaaaaaagctta                                                                      >  1:327324/1‑135 (MQ=11)
                                                                       tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTTGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                     <  1:205034/141‑8 (MQ=11)
                                                                         tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTAc                                                                   <  1:232084/141‑1 (MQ=255)
                                                                           cATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCACaaaacagcgtagtaca                                                                 >  1:327284/1‑129 (MQ=1)
                                                                                   tATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTAATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTa                                                         >  1:12623/1‑141 (MQ=255)
                                                                                    aTCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTAt                                                        >  2:190310/1‑141 (MQ=38)
                                                                                      cGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTAttt                                                      >  1:191059/1‑140 (MQ=39)
                                                                                          atatatCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCATTGTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATtgatga                                                  <  1:103729/141‑1 (MQ=255)
                                                                                           tatatCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATgag                                                 >  1:220074/1‑141 (MQ=38)
                                                                                              atttGAAATATTAAGGGACCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGGTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACg                                              <  2:247691/138‑1 (MQ=255)
                                                                                               tCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGt                                             <  1:300244/141‑1 (MQ=40)
                                                                                                    aaTATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATc                                        <  1:335496/141‑1 (MQ=40)
                                                                                                        ttAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTCCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAGAGCTAATTACAAATGGCGTATTGTTGACACGTACATCaaaa                                    >  1:168859/1‑141 (MQ=255)
                                                                                                                    ggtgTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAAt                        <  2:209413/141‑1 (MQ=40)
                                                                                                                    ggtgTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAAt                        >  2:90997/1‑141 (MQ=40)
                                                                                                                    ggtgTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAAt                        >  2:339417/1‑141 (MQ=40)
                                                                                                                              ttCATCATTCAACGGTCTACCGTTGGGTTCCAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTAttt              <  2:164112/141‑1 (MQ=255)
                                                                                                                              ttCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTAttt              <  2:128590/141‑1 (MQ=40)
                                                                                                                                            |                                                                                                                                          
GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAT  >  CP000730/76213‑76491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: