Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,185 G→A 23.4% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1850GA23.4% 224.6 / 62.0 130intergenic (+243/‑891)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (37/60);  new base A (11/19);  total (50/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

Only 100 of 155 total aligned reads displayed.

AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAA  >  CP000730/76047‑76325
                                                                                                                                          |                                                                                                                                            
aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                            <  1:563086/141‑1 (MQ=255)
    taACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAt                                                                                                                                        >  1:218877/1‑141 (MQ=255)
       cTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                                     <  1:550027/141‑1 (MQ=255)
        tAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTa                                                                                                                                    >  1:9303/1‑141 (MQ=255)
              tgctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTCACaaaa                                                                                                                              >  1:211573/1‑141 (MQ=255)
               gctgcACTTGTGTCACTTAGAACAGCTAACCAAAGTATTAGTAAACCAGGGAATACTAACAGAAAGGCAATTAATTTAATAATTATAGTAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAAAATaaaaaaaa                                                                                                                             <  2:389353/141‑1 (MQ=255)
               gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                             <  2:334920/141‑1 (MQ=255)
               gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                             <  1:681868/141‑1 (MQ=255)
               gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGAGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                             <  1:70596/141‑1 (MQ=255)
                 tgtacatGTATCACTTAAAACAGCTAACCAAAGTGTTAGTAATCCAGTTAATACTAATACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAATTTGAATCTATAGTATAATTTTAACaaaaatg                                                                                                                           <  2:692749/135‑3 (MQ=38)
                        gTAACACTTAGAACCGCCAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGATGCAACGTTGTATATTAACTATAATTTTAACATAAAGGAGtcttc                                                                                                                    >  2:163546/1‑141 (MQ=255)
                         tATCACTAAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTAAAAAATAAAAAAAAAGGAGtcttct                                                                                                                   <  2:566060/141‑1 (MQ=39)
                                tAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCGATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGaa                                                                                                            <  1:507264/141‑1 (MQ=255)
                                 aGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                           <  2:5976/141‑1 (MQ=255)
                                    aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTAt                                                                                                        >  2:656268/1‑141 (MQ=255)
                                    aCAGCGAACCAATGTGTTAGTAATCCAGGGAATCCTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATTGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                        <  1:348060/141‑1 (MQ=255)
                                       gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGACAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                     >  2:266785/1‑141 (MQ=255)
                                          aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATACTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCaca                                                                                                  >  2:110569/1‑139 (MQ=255)
                                           aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAtttacgag                                                                                                 >  1:566722/1‑136 (MQ=255)
                                                    ttAATAATCCAAGGAATACAAACACAAAGGAAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAt                                                                                        <  2:671441/141‑1 (MQ=21)
                                                      aatAAACCAGGAAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                      <  2:708588/139‑1 (MQ=255)
                                                      aGTAATCCATGGAATACTAACACAAAGGCAATTAACCTAATAATTATAGCAAAATACAGGTCCTGTTGCAAAGTTGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                      <  2:617408/141‑1 (MQ=21)
                                                      aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                      >  2:424750/1‑141 (MQ=255)
                                                         aaTCCAGGGAATACTAACACAAAGGTAATTAATTGAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAc                                                                                   <  1:86581/141‑1 (MQ=255)
                                                                         acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                                   <  1:303635/141‑1 (MQ=255)
                                                                          cacaAAGGCAATTAACTTAAATATTACAGCAAAATATATGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGt                                                                  <  1:319662/141‑1 (MQ=17)
                                                                            caAAGGTAATTAATTTAATAATTATAGGAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATTTAAACAATTTAACAAGGATGTTATCACTGTAg                                                                <  1:424903/141‑1 (MQ=255)
                                                                            caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                                <  1:195968/141‑1 (MQ=255)
                                                                              aaGGCAATTACTTTAATAATTCTAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                              <  1:172888/141‑1 (MQ=255)
                                                                              aaGGCAATTAATTTAATAATTATAGAAAAATATAGGTTCTTTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                              <  2:221452/141‑1 (MQ=255)
                                                                               aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                             >  1:399516/1‑141 (MQ=255)
                                                                                ggCAATTAATTTAATAATTATAGCAAAATATAGGTACTGTTGCAAAGTTGAATCTATAGTATAATATTAACAAAAAGGAGTCTGCGGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGt                                                            >  2:228296/1‑141 (MQ=255)
                                                                                            aataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGCAGGTAGGATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                                >  1:574299/1‑141 (MQ=17)
                                                                                             ataatTATAGCAAAATATTGAGTCTGGTGCAAAGTTGATTCTATAGTATAATTATAAAAAAAAGGAGTCTTCTGTATGTACTATTTCAGATATAACCCATTTAACAAGGATGTTATCACTGTAGACGTTGGCTACTATCTa                                               <  2:289655/141‑1 (MQ=14)
                                                                                             ataaaTATAGGCAAACATAGGTTCTGTTGTAAAGTTGAATCTATTGTATAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTa                                               <  2:224301/141‑1 (MQ=11)
                                                                                               aatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTCGGCTACTATCTAAg                                             >  2:32644/1‑141 (MQ=255)
                                                                                                 ttATAGCAAAATATAGGTTCTGTTGCAAAGTTCAATCTATAGTATAATTTTAACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGat                                           <  1:526604/141‑1 (MQ=255)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                     >  1:645850/9‑141 (MQ=1)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                     >  1:156821/9‑141 (MQ=11)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                     >  1:238094/9‑141 (MQ=1)
                                                                                                       aataaatgaGGTTCTGGTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                                     <  2:751178/133‑1 (MQ=11)
                                                                                                        gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATGGTATAATTTTAACAAAAGGGGGTATTCTGTATGAACTATTTCAGAGAGATACGATTTAGCACGGCTGTTATCAAACTAGATGTTGGCTGCTATCTAAGATATGCAtt                                    >  2:674139/8‑141 (MQ=2)
                                                                                                         taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                   <  2:19210/135‑1 (MQ=2)
                                                                                                         taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATAGGCATTg                                   >  1:305848/7‑141 (MQ=1)
                                                                                                         taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTAAAATAATACAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                   <  2:404896/135‑1 (MQ=11)
                                                                                                         gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                   <  1:47979/135‑1 (MQ=0)
                                                                                                         gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                   <  2:768824/135‑1 (MQ=255)
                                                                                                          ttaggaGGTTCTGTTGCAAAGTTAAATTTATAATAAAATATTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                                  <  1:149810/136‑1 (MQ=11)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                 <  2:469028/137‑1 (MQ=2)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                 <  2:649000/137‑1 (MQ=9)
                                                                                                           atatAGGTTCTGTTGTAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                 <  1:236609/141‑1 (MQ=255)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                 <  2:146789/137‑1 (MQ=2)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                 <  1:17344/137‑1 (MQ=9)
                                                                                                            aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                                <  1:70770/138‑2 (MQ=9)
                                                                                                            aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGACTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                                <  2:52961/138‑2 (MQ=0)
                                                                                                             ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                               >  1:558877/3‑139 (MQ=2)
                                                                                                             ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                               >  1:135622/3‑139 (MQ=2)
                                                                                                             ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                               >  1:147746/3‑139 (MQ=0)
                                                                                                             ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATGTTCATTGAggt                               >  1:94504/1‑139 (MQ=255)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                              >  2:267132/2‑141 (MQ=14)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcta                              >  1:269438/2‑138 (MQ=0)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcta                              <  2:501687/140‑4 (MQ=2)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                             >  1:120858/1‑141 (MQ=1)
                                                                                                                 gTTCTGTTGCAAAGTTGACACTCTATTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATAACTGTAGCTGTTGCCTACTATCTAAGATATGCATTGAGTTAtgg                           >  2:761403/1‑139 (MQ=11)
                                                                                                                  ttCTGTTGCAAAGTTGAATCTCTAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                          <  1:253568/141‑1 (MQ=25)
                                                                                                                  tactgctgCAAAGTTGAATGTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGt                          <  2:735252/139‑1 (MQ=11)
                                                                                                                      gtcgCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGata                      <  1:717754/138‑1 (MQ=1)
                                                                                                                       ttccAAAGTTAAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTCATCGTGatat                     <  2:288916/138‑1 (MQ=1)
                                                                                                                         tgaaaGTGGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGatatat                   <  2:776085/139‑1 (MQ=255)
                                                                                                                         gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat                   >  2:250451/1‑141 (MQ=34)
                                                                                                                         gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATAGTGatatat                   >  1:768242/1‑141 (MQ=25)
                                                                                                                          cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                  >  2:128609/1‑141 (MQ=35)
                                                                                                                           aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTACCGGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCt                 >  1:694516/1‑141 (MQ=255)
                                                                                                                              gTTGAATCTATAGTAGAATTTTAACAAAAAGGAGTCTTTTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCATTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa              <  2:381797/141‑1 (MQ=255)
                                                                                                                                tGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGCATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAat            <  1:653370/141‑1 (MQ=255)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt         >  1:244869/4‑141 (MQ=0)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt         >  2:495528/4‑141 (MQ=2)
                                                                                                                                    tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa        >  1:282971/3‑141 (MQ=14)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATTTAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa       <  2:555042/140‑1 (MQ=12)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa       <  2:734253/140‑1 (MQ=12)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa       <  2:425848/140‑1 (MQ=12)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa       <  1:190706/140‑1 (MQ=17)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGGTTTCTGTATGAACTAGTTCGGATATAAACCATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa       <  2:358064/140‑1 (MQ=11)
                                                                                                                                      tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg      >  2:692118/1‑141 (MQ=17)
                                                                                                                                      tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg      <  1:57963/141‑1 (MQ=12)
                                                                                                                                      tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAACTATCGTGATATATCTGAAATATTAAg      >  1:298344/1‑141 (MQ=12)
                                                                                                                                      tataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg      >  1:618780/1‑141 (MQ=17)
                                                                                                                                      tataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg      >  1:162432/1‑141 (MQ=17)
                                                                                                                                      tataATAGAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg      <  1:168564/141‑1 (MQ=12)
                                                                                                                                       ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga     <  2:461006/141‑2 (MQ=17)
                                                                                                                                       ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga     <  1:312343/141‑2 (MQ=12)
                                                                                                                                       ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga     <  1:104054/141‑2 (MQ=12)
                                                                                                                                       ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga     <  1:620681/141‑2 (MQ=12)
                                                                                                                                       ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga     <  1:208718/141‑2 (MQ=12)
                                                                                                                                       ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTAAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg     <  1:227450/141‑1 (MQ=17)
                                                                                                                                        taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACCATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg    <  2:322656/141‑1 (MQ=17)
                                                                                                                                         aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa   <  1:341986/141‑1 (MQ=12)
                                                                                                                                         aGTAGAATTTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCCTTGGCTACGATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTATGGGa   <  1:538689/141‑1 (MQ=11)
                                                                                                                                          gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAGCGTGATATATCTGAAATATTAAGGGaa  >  2:97034/1‑141 (MQ=17)
                                                                                                                                          gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGaa  >  1:111865/1‑141 (MQ=32)
                                                                                                                                          |                                                                                                                                            
AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAA  >  CP000730/76047‑76325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: