Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,651 C→T 15.2% D170D (GAC→GAT)  USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6510CT15.2% 237.0 / 24.5 105D170D (GAC→GAT) USA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (25/62);  new base T (4/12);  total (31/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 106 total aligned reads displayed.

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAG  >  CP000730/110511‑110787
                                                                                                                                            |                                                                                                                                        
aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAACAAATCGAAAACTCTAAGTTCTTTGTGCAAGATGGCGACTTTAAAAATTTGAAAAATTATAAAGAc                                                                                                                                          >  2:196164/1‑141 (MQ=255)
  aaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                        <  1:98901/141‑1 (MQ=255)
   aaatatCCAGTAAAAAAGATCAATAATAAAATCATTCAAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                       <  2:849974/139‑1 (MQ=18)
   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                       <  2:299316/141‑1 (MQ=255)
    gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                      <  1:350147/141‑1 (MQ=255)
    gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                      <  1:427310/141‑1 (MQ=255)
     atatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGGAAAATTATAAAGACGgaga                                                                                                                                     <  1:193359/141‑1 (MQ=255)
      tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGat                                                                                                                                    >  2:219697/1‑141 (MQ=255)
       atCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAAATGAAAAAATAAAAAAAGAAATCGAAAACTATAAGATCTTTGTTCAATATGGCGCCTTTAAAAATTTGAAAACTTATACAGCCGGAGata                                                                                                                                   >  2:815933/1‑141 (MQ=17)
        accGGTAAAAATGGTAGATAAAACAGTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                  <  2:942175/140‑1 (MQ=18)
             ttAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCat                                                                                                                             <  1:308024/141‑1 (MQ=255)
              tAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTCAGTTCTTTGTTCCATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                            >  1:359573/1‑141 (MQ=255)
                aaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGTATAATTATAAAGATGGTGATATTTCAtata                                                                                                                          >  1:53685/1‑141 (MQ=255)
                  aTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAAAAATCAAAAACTGTAAGTTCTTTGTTCAATAGAGAGACTTTAAAAATTTGAAAAATTATAAATAAGGAAATATTTAATATAAt                                                                                                                        >  2:149913/1‑141 (MQ=14)
                    ggTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATca                                                                                                                      >  2:1095027/1‑140 (MQ=255)
                    ggTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTATAAAAATTTGAAAAATAATAAAGACAGAGATATTTCATATAAccc                                                                                                                      >  2:202238/1‑141 (MQ=255)
                       cGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                   <  1:210820/141‑1 (MQ=255)
                       aaaaaataaAATCATTCCAAAAACAGAAATTAAAGAAAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                   <  2:1084815/137‑1 (MQ=17)
                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                 >  1:206429/1‑141 (MQ=255)
                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                 <  1:934446/141‑1 (MQ=255)
                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                 <  1:458559/141‑1 (MQ=255)
                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                 <  2:773268/141‑1 (MQ=255)
                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAAGCCAGAgg                                                                                                                 <  2:1047802/141‑1 (MQ=255)
                         ataataAAATCATTCCAACAAAAGAAATTAAAGACGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTAAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                 <  2:104922/141‑1 (MQ=255)
                            ataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                              >  2:426571/1‑141 (MQ=255)
                              aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTAAAAAATTTGAAAAATTATAAAGAGGGAGATATTTAATATAATcacaaagtgcgt                                                                                                            >  2:224163/1‑130 (MQ=17)
                                aaacATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                          <  1:1016060/138‑1 (MQ=255)
                                aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCTGAg                                                                                                          <  1:796371/141‑1 (MQ=255)
                                  tCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTATAAGTTCTTTGTTCAATATGGCGACTTTTAAAATTCGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAgct                                                                                                        <  2:816909/141‑3 (MQ=255)
                                        caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATATAGACGGAGATATTTCATACAATCCTGAGGCGCCGAGTTATTCgg                                                                                                  <  1:606023/141‑1 (MQ=255)
                                           caaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                               >  1:684975/1‑141 (MQ=255)
                                           caaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGGTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATCTAATCAAGAGGTACCGAGTTATTAGGCTa                                                                                               >  1:564469/1‑141 (MQ=17)
                                           aaaaaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                               <  1:796442/140‑1 (MQ=255)
                                           aaaaaGAAATTAAAGATAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                               <  2:385379/140‑1 (MQ=255)
                                           aaaaaGAAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                               <  2:456985/140‑1 (MQ=255)
                                            aaaaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGTTATTCGGCTaa                                                                                              >  1:391308/1‑141 (MQ=255)
                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCGTATAATACAGAGGTGCCGAGTTATTCGACTAAat                                                                                            >  1:1016517/1‑141 (MQ=255)
                                                gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                          <  1:329274/141‑1 (MQ=255)
                                                 aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTTAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATc                                                                                         >  2:599202/1‑141 (MQ=255)
                                                    ttAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGAGATTTCATATAATCCAGAGGTGCCGAGGTATTCGGCTAAATATCAAt                                                                                      >  1:49690/1‑141 (MQ=255)
                                                       aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGCGGTGCCGGGTTATTCGGCTAAATATAAAtata                                                                                   >  1:366266/1‑138 (MQ=255)
                                                          aTGAAAAAATAAAAAAAGAAAGCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                <  1:589846/141‑1 (MQ=255)
                                                                aaaTAAAAAAAGAAATCGAAATCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGTGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                          <  1:155992/141‑1 (MQ=255)
                                                                aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                          <  1:904964/141‑1 (MQ=255)
                                                                 aaTAAAAACAGAAATCCAAAACTTTAAGTTCTTTTTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                         <  2:75906/141‑1 (MQ=255)
                                                                 aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                         <  1:394998/141‑1 (MQ=255)
                                                                 aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                         <  1:186537/141‑1 (MQ=255)
                                                                   aaaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                       <  2:86368/140‑1 (MQ=255)
                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtag                                                                     <  2:404092/141‑2 (MQ=255)
                                                                      aaaaaGAAATCGAAAACTTTAAGTTATTTGTTCAATATTGCGGCAACTTAGCTTTAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                                    <  1:799338/141‑1 (MQ=14)
                                                                           gAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGAGTTGAAAAATTTGAAAAATTATAAAGACGGAGATAATGCATATAATCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATAATTATAGTGTa                                                               <  2:403167/141‑1 (MQ=17)
                                                                           gAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                               <  1:515985/141‑1 (MQ=255)
                                                                             aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                             >  2:365864/1‑141 (MQ=255)
                                                                                cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTCACTAATGATGATTATAATGTAAAACa                                                          >  2:9193/1‑141 (MQ=255)
                                                                                  aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                        <  2:44359/141‑1 (MQ=255)
                                                                                    aaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGATAAATCTCCATTAACTAGTGATGATTATATTGTaatgtaatta                                                      <  2:534133/141‑9 (MQ=17)
                                                                                    aaCTTTAAGTGCTTTGTTCAATAGGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTa                                                      <  2:378896/141‑1 (MQ=255)
                                                                                     aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                     <  2:1030022/141‑1 (MQ=255)
                                                                                      cTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                    <  2:420551/141‑1 (MQ=255)
                                                                                         tcatgtttttttccAATATTGCGACTTTAAAAATTTGACACAGTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAATTATTCGTCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTCCGCaa                                                 <  2:366266/128‑1 (MQ=14)
                                                                                         tAAGTTCTTTTTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                 <  2:525172/141‑1 (MQ=255)
                                                                                                  tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                        <  1:423496/141‑1 (MQ=255)
                                                                                                  tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                        <  2:573342/141‑1 (MQ=255)
                                                                                                  tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                        <  2:315239/141‑1 (MQ=255)
                                                                                                  tGTACAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAACTACGCAAAAgatatga                                        <  1:405520/141‑1 (MQ=255)
                                                                                                   gTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgat                                       >  1:603532/1‑141 (MQ=255)
                                                                                                   gTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAAAgatatgat                                       >  1:866982/1‑141 (MQ=255)
                                                                                                    ttCAATATGGCGACTTTAAGAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAGAACAATTACGCAAAAgatatgata                                      <  1:703842/141‑1 (MQ=255)
                                                                                                     tCAATATGGCGACTTTACAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgatat                                     <  1:579817/141‑1 (MQ=255)
                                                                                                     tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATACAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgatat                                     >  1:218517/1‑141 (MQ=255)
                                                                                                      cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                    <  2:679234/141‑1 (MQ=255)
                                                                                                             gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAAATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAACATCAATTAACCAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                             <  2:180395/141‑1 (MQ=255)
                                                                                                                aCTTGAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                          <  2:1010753/141‑1 (MQ=255)
                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                         <  1:426571/141‑1 (MQ=255)
                                                                                                                 atttAAAAATTTGAACAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                         <  1:94866/140‑1 (MQ=255)
                                                                                                                   ttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                       <  1:365864/141‑1 (MQ=255)
                                                                                                                    taaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                      <  1:875977/128‑1 (MQ=11)
                                                                                                                      aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                    >  1:389196/12‑141 (MQ=11)
                                                                                                                      aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATACTGTAAAACAATTACGCAAAAGATATGATATACCGACGAGGAAAGCTc                    >  2:116029/1‑141 (MQ=255)
                                                                                                                       agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAAAGATATGATATACCGACGAGTAAAGCTcc                   >  1:170281/11‑141 (MQ=11)
                                                                                                                       agagttaatgaaTTAGAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                   <  2:190420/131‑1 (MQ=14)
                                                                                                                        aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATAGTAAAACAAGGAGAAAAGCTGCa                  >  2:65174/1‑141 (MQ=14)
                                                                                                                          agtGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                <  1:984615/139‑1 (MQ=255)
                                                                                                                            taatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt              <  1:984751/136‑1 (MQ=255)
                                                                                                                              atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAACGACGAGTAAAGCTCCAAAGtga            >  1:469130/4‑139 (MQ=14)
                                                                                                                              aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa            <  1:150900/141‑1 (MQ=255)
                                                                                                                              aaaaaGTATGAAGGCGGAGATGTTTCCTATGATCCAGAGGTGGCGAGTTATTCGGCTAAATTTCAATTAACTAAGGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa            <  2:304920/141‑1 (MQ=18)
                                                                                                                               tgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt           <  2:64854/139‑1 (MQ=255)
                                                                                                                               agaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt           <  1:284307/139‑1 (MQ=255)
                                                                                                                                  aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt        <  2:1057139/141‑1 (MQ=255)
                                                                                                                                     ataAAGATGGAGATATATCATATGATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa     <  2:226601/141‑1 (MQ=255)
                                                                                                                                     ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTATGCCCTTTACGCTAAAGCTTTCTAAGACCGACGAGTAAAGCTCCAAAGTTATTGTTaa     <  1:530369/141‑1 (MQ=14)
                                                                                                                                     ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa     <  2:393653/141‑1 (MQ=255)
                                                                                                                                      taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa    <  1:563778/141‑1 (MQ=255)
                                                                                                                                      taAAGATGGAGAGATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa    <  2:529917/141‑1 (MQ=255)
                                                                                                                                      taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa    <  1:497606/141‑1 (MQ=255)
                                                                                                                                      taAAGACGGAGATATTGCATATAGTCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa    <  2:1104558/141‑1 (MQ=255)
                                                                                                                                        aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg  <  1:138121/141‑1 (MQ=255)
                                                                                                                                        aaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg  >  1:115769/1‑141 (MQ=255)
                                                                                                                                        aaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg  >  1:405795/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAG  >  CP000730/110511‑110787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑