Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 744,511 G→C 5.1% intergenic (+2348/‑578) USA300HOU_0687 → / → sarX hypothetical membrane protein/possible staphylococcal accessory protein X

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730744,5110GC 5.1% 252.9 / 3.4 82intergenic (+2348/‑578)USA300HOU_0687/sarXhypothetical membrane protein/possible staphylococcal accessory protein X
Reads supporting (aligned to +/- strand):  ref base G (43/32);  new base C (2/2);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

ATATTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCAAAATACGAAAAC  >  CP000730/744373‑744641
                                                                                                                                          |                                                                                                                                   
atatTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGAt                                                                                                                                   >  2:290421/1‑141 (MQ=255)
 tatTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGAtt                                                                                                                                  <  2:615935/141‑1 (MQ=255)
 tatTGAACAAGAAAAGCTGATTTTACTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGAtt                                                                                                                                  >  1:484121/1‑141 (MQ=255)
  atTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTa                                                                                                                                 <  1:665038/141‑1 (MQ=255)
    tGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTaaa                                                                                                                               <  1:96885/141‑1 (MQ=255)
            aaaaGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACAtttttt                                                                                                                       >  2:196923/1‑140 (MQ=255)
              aaGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATGACAACTTTGATGGCTTTGGATTAAACATTTTTGAc                                                                                                                     >  1:368578/1‑141 (MQ=255)
              aaGCTGATTTGTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGAc                                                                                                                     <  2:578532/141‑1 (MQ=255)
                 cTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAat                                                                                                                  <  1:677201/141‑1 (MQ=255)
                   gATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTGAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAatca                                                                                                                <  1:53078/141‑1 (MQ=255)
                   gATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAatca                                                                                                                >  1:764744/1‑141 (MQ=255)
                   gATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAatca                                                                                                                <  2:493727/141‑1 (MQ=255)
                        ttCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGGTCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAg                                                                                                           >  2:356456/1‑141 (MQ=255)
                          cTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCa                                                                                                         >  2:578402/1‑141 (MQ=255)
                          cTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCa                                                                                                         >  1:206942/1‑141 (MQ=255)
                           tAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCAt                                                                                                        >  2:397312/1‑141 (MQ=255)
                                 ttGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATg                                                                                                 >  1:629212/1‑141 (MQ=255)
                                  tGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGc                                                                                                <  1:382749/141‑1 (MQ=255)
                                  tGAAAATCATAAACTGCGTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGc                                                                                                <  1:373553/141‑1 (MQ=255)
                                    aaaaTCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAGCATTTTTGACAATCATCAAGAATTTAATCCGa                                                                                              >  1:199933/1‑141 (MQ=255)
                                    aaaaTCATAAACAGCTTAATAATACAGACACATATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGa                                                                                              >  2:128394/1‑141 (MQ=255)
                                       aTCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGc                                                                                           <  2:2392/141‑1 (MQ=255)
                                        tCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACTTTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCa                                                                                          <  1:513261/141‑1 (MQ=255)
                                        tCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCa                                                                                          <  1:2944/141‑1 (MQ=255)
                                        tCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCa                                                                                          <  2:671932/141‑1 (MQ=255)
                                         cATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCAt                                                                                         >  2:636963/1‑141 (MQ=255)
                                          aTAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATc                                                                                        >  1:524323/1‑141 (MQ=255)
                                               cTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTatat                                                                                   <  1:476140/141‑1 (MQ=255)
                                               cTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTatat                                                                                   <  1:475909/141‑1 (MQ=255)
                                                      ataataaAGAACGATATCTAAGTAATAGCGCTCCATTAATTTATAAGACAATAAGGGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACaaaa                                                                            <  1:20974/141‑1 (MQ=255)
                                                      ataataaAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACaaaa                                                                            >  1:288703/1‑141 (MQ=255)
                                                         ataaAGAACGAGATCTAAGTAATAGTGCCCCATTAATTTGTAAGAGATTAAGTGCGCTATATCACAACTTGGATGGCTATGGATTAAACCATTTTGGCAAACATCAAGCATTTACTGCAATGCCACTCAATGAAAAAAATg                                                                         >  2:138564/1‑141 (MQ=255)
                                                           aaaGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGa                                                                       >  1:315083/1‑141 (MQ=255)
                                                               aaCGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAAGCATCAAGCATTGCATGCGATGCCTCTATTTTACAAAAATGAATTTa                                                                   <  2:737436/141‑1 (MQ=255)
                                                                aCGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAACATAGATTTaa                                                                  >  2:399706/1‑141 (MQ=255)
                                                                 cGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATTCGATGCATCTATATGACAAAAATGGATTTaaa                                                                 >  2:286215/1‑141 (MQ=255)
                                                                  gagaTCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTaaaa                                                                >  1:753961/1‑141 (MQ=255)
                                                                  gagaTCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTaaaa                                                                >  1:608243/1‑141 (MQ=255)
                                                                        tAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCCTTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAacac                                                          <  1:593519/141‑1 (MQ=255)
                                                                          aGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGCATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACACCACTa                                                        >  2:752329/1‑141 (MQ=255)
                                                                          aGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGCATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTa                                                        >  2:752257/1‑141 (MQ=255)
                                                                                 gTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATTGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCAGCTATATGACAAAAATGGAGTTAAAACAACAATAGGTCttt                                                 >  1:75271/1‑140 (MQ=255)
                                                                                 gTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTta                                                 <  1:469052/141‑1 (MQ=255)
                                                                                  tGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTtat                                                <  1:623857/141‑1 (MQ=255)
                                                                                  ggCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTtat                                                <  1:227838/140‑1 (MQ=255)
                                                                                    cTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACAATTTTGACAATCATCAAGCATTTAATGCGATGCATCCATATGACAAAAAAGGATTTAAAACAACACTAGGACTTatat                                              >  2:618234/1‑141 (MQ=255)
                                                                                      ccATTAATTTATAAGACATTAAATGCGCTGTATAACAACTTTGATGGATTTGGATTACAAATATTTGACAACCCTTAACCATATAATGCGAAGGATCGATAGCACAAAAAACGATGTAAAACAACCCGACGTCTTATATTa                                            >  2:752486/1‑141 (MQ=255)
                                                                                          tAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGaaa                                        >  1:357913/1‑141 (MQ=255)
                                                                                          tAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGaaa                                        <  1:636963/141‑1 (MQ=255)
                                                                                           aaTTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGaaaa                                       >  2:699685/1‑141 (MQ=255)
                                                                                            aTTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAg                                      <  2:298856/141‑1 (MQ=255)
                                                                                            aTTTATAAGACATTAAGCTCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAg                                      <  1:291908/141‑1 (MQ=255)
                                                                                             tttATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGt                                     >  1:669318/1‑141 (MQ=255)
                                                                                              ttATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGtt                                    >  1:20638/1‑141 (MQ=255)
                                                                                               tataAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGttt                                   <  2:402208/141‑1 (MQ=255)
                                                                                                   aGACATTAAGTGCGCTGTATCACAACTTTGATTGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCg                               <  2:468730/141‑1 (MQ=255)
                                                                                                   aGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCg                               <  1:155994/141‑1 (MQ=255)
                                                                                                    gACATTACGTGCGCTGTATCACAACTTTGATGGCTTTGCATTAACAATTCATGACAATCATCAAGCCATTATATTGATGCATCTATTCAAGGATAATGGATGTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGa                              <  1:752329/141‑1 (MQ=255)
                                                                                                    gACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGCATCATACATTTGTGACAATCATCAAGCATTGTGCTGTATGCAAATTCATTGCAAAAATGTATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGa                              <  1:752257/141‑1 (MQ=255)
                                                                                                        ttAAGTCCTCTGTATCACAACTTTCATGCCTTTGAATTAAACATTTTTTACAATCATCAAGCATTTAATGCGATGCATCAATATAACAAAAATTGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAatat                          <  1:223531/141‑1 (MQ=255)
                                                                                                         tAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGAAAATCATCAAGCATTTAATGCGATGCATATATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAatatt                         >  2:233271/1‑140 (MQ=255)
                                                                                                          aaGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGt                        >  2:169840/1‑141 (MQ=255)
                                                                                                          aaGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGt                        >  1:295102/1‑141 (MQ=255)
                                                                                                            gTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGtct                      >  1:691180/1‑141 (MQ=255)
                                                                                                            gTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGtct                      >  2:221621/1‑141 (MQ=255)
                                                                                                            gTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGtcc                      >  1:9050/1‑140 (MQ=255)
                                                                                                            gTGCGCTGTATCACAACTTAGATGGCATCGGAATAAACATTTTTGACAATCAACAAGCATTTAAAACGATGCATCTATATGACAAAAATGGAATTAAAACAACACCAAGACTTATAATAGAACAGATTATCGAataagacc                      >  2:88196/1‑136 (MQ=255)
                                                                                                              gcgcTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCg                    >  2:605819/1‑141 (MQ=255)
                                                                                                                gcTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATATCTCGaa                  >  2:182218/1‑141 (MQ=255)
                                                                                                                aacatATCACAAGCTTGATGGCTTTAAAGGAAACACGTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGaa                  <  1:356456/137‑1 (MQ=255)
                                                                                                                   gTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAAcc               <  1:658089/141‑1 (MQ=255)
                                                                                                                   gTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAAcc               <  1:290421/141‑1 (MQ=255)
                                                                                                                      tCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCaaa            >  1:644791/1‑141 (MQ=255)
                                                                                                                      ccacaGCTGTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCaaa            >  2:196924/2‑141 (MQ=255)
                                                                                                                       tacaACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTGAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGACAAGTTTATCGAATATGTCTCGAAACCaaaa           <  2:181216/140‑1 (MQ=255)
                                                                                                                       cacaACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCaaaa           >  1:334746/1‑141 (MQ=255)
                                                                                                                        acaACTTTGATGGCTTTGAATTAAACATTATTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCAAAAt          <  1:337008/141‑1 (MQ=255)
                                                                                                                         caACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCAAAATa         <  1:585852/141‑1 (MQ=255)
                                                                                                                           aCTGTGATGGCTTTGGATTAAGCATTTTTGCCAATCATCAAGCATTGAATTCGATGCGTCTATATGACACAAATGGATGTAAAACAACACTAGGGCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCAAAAGAct       <  2:199933/141‑2 (MQ=255)
                                                                                                                                gATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTGGAAAAGTTTATCGAATATGTCTCGAAACCAAAATACGaaaaa  >  2:23390/1‑140 (MQ=255)
                                                                                                                                gATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCAAAATACGaaaaa  >  2:409520/1‑140 (MQ=255)
                                                                                                                                gATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCAAAATACGAAAAc  >  1:433370/1‑141 (MQ=255)
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ATATTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCATTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTAGAAAAGTTTATCGAATATGTCTCGAAACCAAAATACGAAAAC  >  CP000730/744373‑744641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: