Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,516 C→A 31.3% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5160CA31.3% 121.5 / 58.8 86Q196K (CAA→AAA) USA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (31/27);  new base A (13/14);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

AGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTG  >  CP000730/111379‑111654
                                                                                                                                         |                                                                                                                                          
aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                                         >  1:520619/1‑141 (MQ=255)
    aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTTTCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                                     >  2:706482/1‑141 (MQ=17)
      ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGGTGGAGATATATCATATAATGCAGAGGTGCCGAGTTATTCAGCTAAATATAAAt                                                                                                                                   >  2:416901/1‑141 (MQ=255)
      ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAt                                                                                                                                   >  2:760850/1‑141 (MQ=255)
       tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCACATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAtt                                                                                                                                  <  1:542119/141‑1 (MQ=255)
         aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                                >  1:485942/1‑141 (MQ=255)
          agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCTGCTCAATATCAATTAAc                                                                                                                               >  2:106703/1‑141 (MQ=255)
          agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                               <  1:601205/141‑1 (MQ=255)
           gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                              >  1:266128/1‑141 (MQ=255)
            aagacAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTCTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAACGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTa                                                                                                                             <  2:349831/141‑1 (MQ=255)
            aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCGATATCAATTAACTa                                                                                                                             <  1:405873/141‑1 (MQ=255)
              aaaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                           <  1:514710/140‑1 (MQ=255)
                 aaCATAAAAAAAGAAATCGAAAACTTTATGTTGTGTGCGCAATATAGCAATTTTAAAGGTTTAATGAATTATAAAGATGGAGATATATCCTATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                        <  1:527118/141‑1 (MQ=17)
                  aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTTGCAATTTTAAAGATCTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                       >  1:70502/1‑141 (MQ=17)
                    aTAAAAAAAGAAATCGAAAACTTTAAGGTTTTTGCGCAACATAGCAATATGAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAAGTGCCGAGTTATTGAGCTCAATATCAATTAAGTAGagatgag                                                                                                                     >  2:702734/1‑140 (MQ=18)
                     aaaataaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGCTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAtt                                                                                                                    <  1:62973/140‑1 (MQ=255)
                       aaaaaaaaaaCCGAAAACTTTAAGTGTGTTGCACAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGAGATATCATATAATCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                  <  2:349887/141‑1 (MQ=17)
                       aaaaaaGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTTTCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAACAAATAACTAATAATAATtat                                                                                                                  >  2:523784/1‑141 (MQ=17)
                       aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                  <  1:193133/141‑1 (MQ=255)
                       aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                  <  2:744473/141‑1 (MQ=255)
                       aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                                  <  2:446761/141‑1 (MQ=255)
                       aaaaaaGAAATCGAAAACTTGAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                  <  1:607749/141‑1 (MQ=255)
                       aaaaaaGAAATCGAAAACTTCCGGTTTTTGGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATaat                                                                                                                  >  1:756397/1‑141 (MQ=18)
                        aaaaaGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTTCCAATGTTAAAGATTTAATGAATTATAAAGCTGGAGATGTATCATATAATCCAGTGTTTCCAAGTTATTCAGCTAAAAAAAAATAAACTAATGTTAAtttat                                                                                                                 >  2:377886/1‑138 (MQ=12)
                        aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATTATtata                                                                                                                 >  2:577917/1‑141 (MQ=255)
                        aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtata                                                                                                                 >  2:497439/1‑141 (MQ=255)
                          aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                                               >  1:119885/1‑141 (MQ=255)
                          aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                                               >  1:637013/1‑141 (MQ=255)
                           aaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                                                              <  2:454037/141‑1 (MQ=255)
                              aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAAAAATTAACTAATGATGATtaaagggaaa                                                                                                           >  1:170918/1‑133 (MQ=18)
                              aaaTCGAAAACTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                           <  2:578669/141‑1 (MQ=255)
                              aaaTCGAAAACTATAAGTTTCTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATTCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                           <  2:142164/141‑1 (MQ=17)
                               aaTCTAAAACTTTAGATTATTTTCTCTATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAATATAATGTaaa                                                                                                          >  2:756141/1‑141 (MQ=18)
                                  ggAAAACTTTAAGTTTTGTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                                       <  2:611521/140‑1 (MQ=255)
                                  cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGAAGATTATAATGTAAAACa                                                                                                       >  2:642561/1‑141 (MQ=255)
                                   gAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGGGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGAAGATTATAATGTAAAACaa                                                                                                      >  1:126457/1‑141 (MQ=255)
                                   gAAAACTTTAAGTTTTTTGCGCAATATAGCAATATTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                                                                      >  2:695955/1‑141 (MQ=255)
                                    aacaCTTGAAGTTTTTTGCGCAATATAGCAATTTTACAGATATAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGTGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                     <  1:212957/138‑1 (MQ=17)
                                    aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                     <  2:583141/141‑1 (MQ=255)
                                       aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAAGGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                                  <  1:287480/141‑1 (MQ=255)
                                        cTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                                 <  2:608626/141‑1 (MQ=255)
                                          ttAAGTTTTTTGCGCAATATAGCAATTTTACAGCTTTAATGAATTATACAGATGCAGATCTATCCTCTCATCCAGAGCTGCCGAGCTATTAGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACgca                                                                                               >  2:38187/1‑139 (MQ=17)
                                           tAAGTTTTTTGCGCAAAACAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACgcaa                                                                                              <  1:760184/141‑4 (MQ=17)
                                            aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                             >  1:167278/1‑141 (MQ=255)
                                                tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGat                                                                                         >  2:83761/1‑141 (MQ=255)
                                                  tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGatat                                                                                       >  2:410376/1‑141 (MQ=255)
                                                   ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGGGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAGCTAATGATGATTATAATGTAAAACAATCAAGTAAAAGagaag                                                                                      >  2:411294/1‑137 (MQ=21)
                                                   ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATg                                                                                      >  2:425997/1‑141 (MQ=255)
                                                     gcgcAATAGAGCAATTTTAAAGAGTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAc                                                                                    <  1:191837/141‑1 (MQ=255)
                                                      cgccaagaaGCGATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                                   <  2:529765/133‑3 (MQ=11)
                                                       gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAACAGATATAACaa                                                                                  >  2:723512/1‑140 (MQ=255)
                                                        ttttttttcAATTATAAAGATTTAATGAATTATAAAAATGGAGATGTATCATATAATCTGGAGGTGCCGAGTTATTTATTTTATTATTATTTAAATAATGATGATTATAATGTAAAACAATTACGTAAAAGATTTgtaata                                                                                 >  2:745590/9‑136 (MQ=12)
                                                        cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                                                                 >  1:598968/1‑141 (MQ=255)
                                                           aataGCTATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCACAAGATATGATATAccg                                                                              >  2:69479/2‑140 (MQ=14)
                                                              aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTTTAATGTAAAACAAGTACGTAAAAGATATGACATACcaaca                                                                           >  1:440408/1‑141 (MQ=255)
                                                                aaaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacgag                                                                         <  1:470544/140‑7 (MQ=11)
                                                                      tAAAGGTTAAAAGAATTATAAAGATTGAGATATAGCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                                   <  2:593399/141‑1 (MQ=17)
                                                                        aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                 >  2:444928/1‑141 (MQ=255)
                                                                          gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCATTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                               >  2:51392/1‑141 (MQ=255)
                                                                          gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                               <  2:763239/141‑1 (MQ=255)
                                                                            tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                             <  2:377912/141‑1 (MQ=255)
                                                                                 tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                        <  1:523784/141‑1 (MQ=255)
                                                                                 tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATATAAAACAATTACGTAAAAGATATGACATACCAAGAAATAAAGCGCCGAAGCtgt                                                        <  1:491838/141‑1 (MQ=255)
                                                                                   aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                      >  1:270730/1‑141 (MQ=255)
                                                                                    aTTATAAAGATGGTGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAAAAGAAGGAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                                     <  1:360219/141‑1 (MQ=17)
                                                                                            gATGAAGATATATCATATAATCCAGATGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                             <  2:596509/141‑1 (MQ=255)
                                                                                                  gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                       <  1:676119/141‑1 (MQ=255)
                                                                                                   atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAAAGGGa                                      >  2:435717/1‑141 (MQ=255)
                                                                                                      tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                   >  1:652611/1‑141 (MQ=255)
                                                                                                       atCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAttt                                  <  1:57766/141‑1 (MQ=255)
                                                                                                              aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                           <  1:165018/141‑1 (MQ=255)
                                                                                                               aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                          <  1:703660/141‑1 (MQ=255)
                                                                                                                 ccAGAGGTGCCGGGTTATTCAGCTCAATAACAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGGATTTAAAAGGTtca                        >  2:128182/1‑141 (MQ=255)
                                                                                                                    gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca                     >  1:723824/1‑141 (MQ=255)
                                                                                                                       gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                  >  1:373133/1‑141 (MQ=255)
                                                                                                                        tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCCTCAGGTg                 >  1:127959/1‑141 (MQ=255)
                                                                                                                         gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTCTAATGGAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                >  1:332461/1‑141 (MQ=255)
                                                                                                                            gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAGTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata             >  1:374898/1‑141 (MQ=255)
                                                                                                                               ttATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa          <  1:746380/141‑1 (MQ=255)
                                                                                                                                tATTCAGCACAAACTCAAATAACTAATGATGATTATAATATAAAACAATTACGAAATAGATATGACATACCAACAAATAGAGCTCCGAAGCTATTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa         <  2:440408/141‑1 (MQ=14)
                                                                                                                                 aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAAAAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaa        >  1:613706/1‑141 (MQ=255)
                                                                                                                                  ttCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa       <  2:175287/141‑1 (MQ=255)
                                                                                                                                    cAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCGACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaa     >  1:521551/1‑141 (MQ=255)
                                                                                                                                      acTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAtt   <  2:587559/140‑1 (MQ=255)
                                                                                                                                       cTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTg  <  1:611795/141‑1 (MQ=255)
                                                                                                                                       cTCAATATCAATTAAATAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTg  <  2:287410/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
AGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTG  >  CP000730/111379‑111654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: