Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,187,152	0	T→G	100.0%	1.4	2	L215V (TTG→GTG)	insD1	IS2 transposase TnpB
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/2):  ref base (0/0):  total (0/2)

AACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTAT  >  NC_000913/3187130‑3187431                       |                                                                                                                                                                                                                                                                                        aaccgggcAGCTCGCCCAGATGGTGGGGCTTGAACCAAAGAACACGGCGGTGCGGAGGCCGTGAAGTAACGGAATAGCAGAGAGCTGCGGGAAATCGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGCCGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATGCGCATAGTGCGCTGGGTTATCGCTAGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTa   <  2:305760/294‑1 (MQ=11)  acacGCGAGTTCGCACGGATGGTGGGACTTGAACCGAAGAATATGGTGGCGCGGAGTCCGGAGAGTAAAGGAATAGCAGAGAGCTTCGTGAAAACGATAATGCGTGACTACATACGCATCATGTCCAAACCATGTGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGGTCGAGCATTATAACTAATGGCATCCGCATAGTGCGCTTGTTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTat  <  2:171435/301‑1 (MQ=11)                       |                                                                                                                                                                                                                                                                                        AACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTAT  >  NC_000913/3187130‑3187431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.