Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,950,469	T→G	22.9%	intergenic (+49/‑38)	ilvL → / → ilvX	ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,950,469	0	T→G	22.9%	11.8 ‑1.4 ‑0.2	36	intergenic (+49/‑38)	ilvL/ilvX	ilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  new base (3/6):  ref base (16/11):  total (19/17)
Fisher's exact test strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.29e-01

CTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATGCCGACATGAGCAGGGTGCGGCAATGGCGGCTATCGGTTATGCTCGTGCTACCGGCAAAACTGGCGTATG  >  NC_000913/3950179‑3950759                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                   ctATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGCTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACTAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGCGGTTTTTTTGa                                                                                                                                                                                                                                                                                           >  2:287151/1‑300 (MQ=255)                                   aaaTTATTAAACGCAACATAAAAATCGGGCAAAAAAAAACTTGTAATATTTACAAAAACTATGGTAACTCCTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                                                                                                                                                                                                                        <  1:287145/301‑1 (MQ=255)                                      ttattaAACGCATCATAAAAATCGGCCAAAAAATATCTTGGACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGAGTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                                                                                                                                                                                                                      <  2:399682/301‑1 (MQ=255)                                                              gCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCCAGGACCAGACAATGACTAACAGTACAAAGTTCTGTTTCCCaataa                                                                                                                                                                                                                             >  1:153949/1‑298 (MQ=255)                                                                        atCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAAAGACCAAAATTCAGTTTCTCAAGATTCAGGACgg                                                                                                                                                                                                                    >  2:160305/1‑301 (MQ=255)                                                                                 tcattACAAAACCTAGAGGAACTCTTTTGGCATTACTTCGAACAAGATGAAAGAAAAGACAAAATGACAGCCCTTCGACGAGTGATTAGCCTGGTCGTGATTAGCGGGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaact                                                                                                                                                                                                          <  1:53946/298‑1 (MQ=255)                                                                                  aTTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATACCAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAactaacta                                                                                                                                                                                                         >  2:246326/1‑301 (MQ=255)                                                                                          aaCCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAATAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACCTAAACGAGGAGCAGACAATGAATAATGTCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGCACTACCTATGAATgg                                                                                                                                                                                                  >  2:71784/1‑300 (MQ=255)                                                                                           aCCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGAGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCCAGCCTTAACGAACTAAGACCCCCGCACCGAACGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGCCGCTGGATAAAAGCACAAAAGTCAGTTGCCCAAGATTACGGACAGGGACCCAACTAGAAATGgcg                                                                                                                                                                                                >  1:12477/1‑301 (MQ=255)                                                                                                      ctctTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCCCCGAAAGGTCC‑GGGGGTTTTTTTTGCCCTTAAAAACATAACCGAGGAGCAGACAATGa                                                                                                                                                                                                                                                           >  1:19295/1‑231 (MQ=255)                                                                                                      ctctTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGCCCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                                                                                                                                                                                                                      >  1:78618/1‑236 (MQ=255)                                                                                                      ctctTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTATTTCTCAAGATTCCGGACGGGGAACTAACTATGAATGGAGCCAAgggggtg                                                                                                                                                                                     >  1:263258/1‑301 (MQ=255)                                                                                                      ctctGTAGGCATTGCTACGAACAAGATGCAAGAAAAGACAAAAGGACCGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGa                                                                                                                                                                                                                                                           <  2:19295/231‑1 (MQ=255)                                                                                                      ccctTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTTGTGATTATTATCCCACCGTGTGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                                                                                                                                                                                                                      <  2:78618/234‑1 (MQ=255)                                                                                                                   cctttcaaCAAGATGGTATAATAGACAAAATGTCAGCCCTTCGACGAGTGATTTGCTTGGTCGGTGTGAGCGTGGTGGTGATTATTATCCCCACGTGCGGGGGTGCACTTGGATGAGGAAATGTATATAGATCAAGCCGTAAAGAACTAACACACCCGCACCGAAAGGTGA‑GGGTGGTTTCTTTGAGCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAGTCTGTTTCGCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCg                                                                                                                                                                            <  2:249415/291‑1 (MQ=255)                                                                                                                   ccTTCGAACAAGCTGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCTTGGTCGTGATGAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGCAAGGCTTCGAGATCAAGCCTTAACGAACTAAGACCCCCGCAACGAACGGTCC‑GGGGGTTTTTTTTGGCCTTAAAACCATAACCGAGGAGCAGACAATGAATAACCGCACAACCTTCTGTTTCGCACAATTCAGGACGGTGAACTAAATATGAACGGCACACAGAGTGTGGTACATgag                                                                                                                                                                            >  1:249410/1‑295 (MQ=255)                                                                                                                   acTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGAc                                                                                                                                                                                                                      <  1:357125/254‑1 (MQ=255)                                                                                                                              aaggCAAGAAAAAACAAAATGACAGGCCTTCTACGAGTGATGAGCCTGGGCGTGATTAGCGTGGTGGTGATTAGTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTGTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAggg                                                                                                                                                              <  1:160304/298‑1 (MQ=255)                                                                                                                               aTGAAAGAAAAGAAAAAATGACAGCCCTTCTACGAGTGATTAGCCGGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAggg                                                                                                                                                              <  1:246321/299‑1 (MQ=255)                                                                                                                                      aaaaGACAAAACGACAGCCCGTCTACGAGTGATTTGCATGGTCGTGAGTAGCGTGATGGTGATTATTCACCCACCGTACGGGGCTGCACTTGGACTAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTGTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAac                                                                                                                                                       <  2:153950/299‑1 (MQ=255)                                                                                                                                         aGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCCCAAAATTCTGATTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGGGGTCCATGCGTTGCGGGCACAGGGTGTGAACACCgg                                                                                                                                                  >  1:156613/1‑300 (MQ=255)                                                                                                                                                                   tGATTAGCCTGGTCTTGATTAGCGGGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGaa                                                                                                                                                                                                     <  1:381455/224‑1 (MQ=255)                                                                                                                                                                   tGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATACCACCGTGCGGGGCTGCACTTGGACGAGGAACGGCTTAGAGATAAAGCCTTAACGAACTAAGACCCACGCACCGAAAGGTCC‑GGGGGTGTTTTTTGAACTTAAAAACATAACCGCGGCGCAGACAATGAATAACAGCACAACATTCTGTTACACAAGATTCAGGACGGGGACAAAACTATGaa                                                                                                                                                                                                     >  2:381464/1‑224 (MQ=255)                                                                                                                                                                      ttAGCCTGGTCGTGATTAGCGTGGTGTTGAGTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGc                                                                                                                     <  2:263263/301‑1 (MQ=255)                                                                                                                                                                                  tGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAACCCCGCACCGAAAGGTCC‑GCGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGACGACAATGGATAACAGCACAACGTTCTGTTTCTCAAGTTTCAGGACGGGGAACTAACTATGAATGGAGCACAGTGGGTGGTACATGCGTTGCGGGCACATTGTGTGAACACAGTATTCGGTTATCCGGGGGGCACAATTATGCCAGTTTACGATg                                                                                                         >  2:282403/1‑301 (MQ=255)                                                                                                                                                                                        gCGTGGGGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGcac                                                                                                                                                                  <  1:207551/238‑1 (MQ=255)                                                                                                                                                                                          ttggttgtgATTATTATCCCACGCTGCGGGGATGCACGTGGACGAGGAAATGCTTAGAGATCAAGCCTGAAAGAACTAACACCCCCGCACCGAAAGGTCC‑GGGGTGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTAt                                                                                                 <  1:415371/300‑1 (MQ=255)                                                                                                                                                                                                      attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGACCTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTCTCCGGGTGGCGCACTTATACCGGGTTAAGAGGCATTGTATGAAGgcggcgg                                                                                      >  2:289664/1‑300 (MQ=255)                                                                                                                                                                                                         atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGt                                                                                                                                                  <  1:227054/237‑1 (MQ=255)                                                                                                                                                                                                         atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTAAGGACGGGGAACTAACTATGAATGGCGCACAGTGTGTGGTACATGCGTTGCGGGCACAGGGTGCGAACACCGt                                                                                                                                                  >  2:227059/1‑237 (MQ=255)                                                                                                                                                                                                                           gccagtGGACGAGGAAAGTCTGAGAGATCAAGCCTTAACGAACTACAACCACCGAACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCCCAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGAACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATGCCGACa                                                                 <  2:156614/295‑1 (MQ=255)                                                                                                                                                                                                                                             gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGGGGGGCACTTGCTATGCCCACATGGCCGGGGTGCgggcagg                                              >  1:202421/1‑296 (MQ=255)                                                                                                                                                                                                                                                  gagaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACATTAAAAACATAACCGAGGAGCAGAAAATGAATAACAGCACAAAATTCTGTTTCTCAAGAATAAGGACGGGGAACGAACTATGAAAGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGGTTTCGGTTATCCGGGTGGCGCAATTATGACGGTTTACGATGAAGTGTATTACGGCGGCGTGGAGCACTTGCTATGCAGAACGAAACAGGGGTCGGCAATggcggg                                         >  2:180083/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           cccccGCACCGAAAGGTCC‑GGGGGTTTGTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTAc                                                                                                                                                                                 <  2:150863/140‑1 (MQ=255)                                                                                                                                                                                                                                                                           cccccGCACCGAAAGGTCC‑GGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTAc                                                                                                                                                                                 >  1:150862/1‑140 (MQ=255)                                                                                                                                                                                                                                                                                         ggTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACAGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACAACGTTTTCGGTTATCCGGGTGGGTCAGTTCTGACGGGTTACGATGCATTTTATGACGTCGGCGTGGAGCCATTGCTATGGCGACTTGAGAAGGGTGCGGCGATGGCGGCTATGGGTTCTTGTCGTCCTACCGGCaagcatggggtcgg  >  2:162765/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                   CTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATGCCGACATGAGCAGGGTGCGGCAATGGCGGCTATCGGTTATGCTCGTGCTACCGGCAAAACTGGCGTATG  >  NC_000913/3950179‑3950759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.