Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,334,502	C→A	10.1%	R265S (CGC→AGC)	ispB →	octaprenyl diphosphate synthase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,334,502	0	C→A	10.1%	4.2 0.0 ‑0.9	41	R265S (CGC→AGC)	ispB	octaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (17/20):  total (19/22)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.21e-01

GCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAAT  >  NC_000913/3334203‑3334797                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                        gcgTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGATGCCGCTGCTGCATGCGATGCCACATGGCACACCA‑GAACAGGCACAGAAGATCCTGAACACAATCGAACAGGGTAACGGACg                                                                                                                                                                                                                                                                                                        >  1:314046/1‑301 (MQ=255)    ttgtctatagtcagtccGCGCGTCTGTTTGAGGCGGCCGAGCAGTGGTCGGGGATTCTAGCTGGCTGTTCGCCGTAGGAGTAGAAAGGCCTGCAGGATTCTGGGCGCTATCTCGGCACTGCTTTCCAGTAGAGCGACGATTTACTCGATTACAAAGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACAAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCa                                                                                                                                                                                                                                                                                                     <  2:84270/286‑1 (MQ=255)                aaaccgctgtgcggTTTGAGGCTGCCGTGCAGTGTTCCGGGATTCGGGCTGGCTGTACGCCTGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTAGCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAAcc                                                                                                                                                                                                                                                                                         <  2:356143/288‑1 (MQ=255)                                      atgagcgtactgttcgtggctggcggctggagGGACGCCGGGGGAGGGGTAAGGAATGCAGGAATATGGGCGCTATCTCGGCACTGCTTTACAGTTGAACTAAGAGTGACTCTGTTACAAGGCCGATGGCGAACAGTTAGGTAACAATGTCGGCGAAGATCTGAACGAAGGTAAACCCACGCTGCCGCTGCTGCATGCTATGCATCAGGTCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACATGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGc                                                                                                                                                                                                                                                                    <  2:414715/276‑1 (MQ=255)                                       gCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCtt                                                                                                                                                                                                                                                                  >  1:75145/1‑301 (MQ=255)                                          gcgcAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCCTCTTCTGGACCCGGTTCTGGAAGCCATGAACGGCtgtg                                                                                                                                                                                                                                                               >  1:343501/1‑301 (MQ=255)                                                tgtTCCGCGACTCGGGGTGTCTGTTCGCCGGATGAGTGTACATGCCTGCAGGATTATGGGCGCCAACTCGGCACTGCCTTACAGTTGACCGACGATTTACTCGATTAACACTCCTATGGCGAACAGTTAGGTAAAAAGGCCGGTGACCATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTTCCTGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACTGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGAt                                                                                                                                                                                                                                                            <  2:346200/298‑1 (MQ=255)                                                       ggATTCTGGCTGTCTGTACGCAGGAGGAGGAGAAAGGCCTTCAGGATTATGGGCGCGATCTCGGAACTGCTGTCCAGTTGATCGCCGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAAt                                                                                                                                                                                                                                                   <  2:314054/300‑1 (MQ=255)                                                                   gctgTACGCCGGAGGATGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTATGACACCGGTTCGGGAAGCACTGCACCCATGTGGATCCCTTGCATGGACGCCTccggg                                                                                                                                                                                                                                      >  1:394896/1‑297 (MQ=255)                                                                                   ggagAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCTATTACAATGCCGATGGCGAACAGTGCGGTAAAAATGTCGGCGACGACCTGAACGAAGGTAAACCGACGCTGCCGCTGATGCATGCGAGGCCTCATGGCGCACGA‑GAACAGGCACAGATGATCCGTACGGGCCTCGCACGGGGTAAGGGTCGACATCTTCTGTAACCGGACCCGGCAGCAATGCAAGCTTGTGGAGCACTTGATTGGCCGGGTCAGCGTGCCAAGgagaacgca                                                                                                                                                                                                                      >  1:250322/1‑294 (MQ=255)                                                                                                                                   ttggATCGACGGTGGACTGGATTAAAAGGCCGAGGGAGAACAGTTAGGTAAAAATGTCGGGAACGATCTGAACGAAGGTAAACCGACGGTGCCGGTGCTGCATACGATGCATCATGGCAGACCA‑GAACAGGAACAGATGATCCGTACCGCGAGCGAACAGGGTAACGGTCGCCAGCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGGGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACAccc                                                                                                                                                                              <  2:100197/290‑1 (MQ=255)                                                                                                                                   gTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCCCTTGAATGGACGCGTCCGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGAAGCGTTACAGGTGCCCCCGGACAccc                                                                                                                                                                              >  1:100197/1‑293 (MQ=255)                                                                                                                                      gATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCACCGAACAGCGTAACGGTCGCCCTCTTCTGGACCCGGTTCTTGAAGCAATGCACGCTTGTGGATCTCTTTATTGGCCGCTCCACCCTGCCGCGGCAGAACCCGACCACTCCCTCGCACCCTTCCCGGTGCTccccgccccaccttcgacagaa                                                                                                                                                                   >  1:221222/1‑282 (MQ=255)                                                                                                                                              attgaagcgagtagacagcaaagtgCGACCAGTTAGGAAAAAATGTCGCCGAGGAGCTGAACGAAGGTACACCGCTGCTGCCGCTGCTGCATGCGATGCCTCATGGCACAACA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAAGGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTc                                                                                                                                                             <  2:61492/275‑1 (MQ=255)                                                                                                                                               tttACTAGACTACAACGCCGAAGTCGAACAGTTAGGTAAAGATGTCGGCGAAGATCTGAACGAAGGGAAACCGCCGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAATAGGCACAGATGATCCGTACCGCCAGCGAACAGGGCAACGGTCGCCATCTTCTTGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGTCGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATc                                                                                                                                                          <  2:75145/301‑1 (MQ=255)                                                                                                                                                   cTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGACCAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTTGAAGACATGAAAGCTTGCGGATCTCTTGAATGGACGCGTCAGCTTGCCGAGGCAGAAGCCATCCAAGCCATCGCATCGTTACCGGTGCCTCCGGACCCCCCTTgtctggaaccacccacggac                                                                                                                                                       >  1:15925/1‑281 (MQ=255)                                                                                                                                                               gCCGATGGCAAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCCTGTGGATCTCTTGAATGGACGCTGCAGCGTGCCGAGGAAGAAGCAGACAACGCACTCGCAGCGTTACCGGTGCTCCCGGACACCCCGTGGCGAGGACCACTCATCGGCCTccccccccac                                                                                                                                           >  1:168513/1‑291 (MQ=255)                                                                                                                                                                     ggCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGCATGGACGCGTCAGCGTGCCGGGGAAGAAGCAGACCACGCCATCGCAGCGTTACAGGTGCTCCCGGACCCCCCTTGGCGAGACGCACTCATCGGCCTCGCGCACATCGCTGtt                                                                                                                                     >  1:149335/1‑300 (MQ=255)                                                                                                                                                                      gggAAAAGTTAGGTAAAAATGCGGGCGACGAACGGAACCAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCAGCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTAGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTc                                                                                                                                    <  1:332852/298‑1 (MQ=255)                                                                                                                                                                           cAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACAGTAGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCCCCCACCGCTGTTCCAcgcg                                                                                                                              >  1:202462/1‑301 (MQ=255)                                                                                                                                                                                                   gaTCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGCCACCCCTTGGCGAGACGCACTCATCGGCCTCGCGCCCATCGCTCTTCAACCCGATCGTTACTCCCCCCCCCCCATc                                                                                                       >  1:215427/1‑300 (MQ=255)                                                                                                                                                                                                    aTCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTAGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTCCAGGTGCTCCAGGacac                                                                                                                                                                                >  1:179793/1‑226 (MQ=255)                                                                                                                                                                                                    aTCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTAGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCAGGacac                                                                                                                                                                                <  2:179794/226‑1 (MQ=255)                                                                                                                                                                                                                              cctccgaggcatgacaggaaggatGGCACACCA‑GAAAAGGCACAAATGGGCAGTACCGCCATCGAACAGGGTAGCGGTCGCCATTTTCTGAAACCGGTTCAGCAAGCAATGAACGCTTGTGGATCTTTGGAATGGACGCGTCAGCAGGGCGAGGAAGAAGCAGACAAAGCCATCTCAGCTTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGAACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                           <  2:15925/279‑1 (MQ=255)                                                                                                                                                                                                                               cgatgcagcAAGCGATGCATAAGGGGACACAA‑GAACAGGCACGGATGATCCGTAACGCCAGCGAACAGGGTAACAGGAGCCATCTTCTGGAAACGGTGCGGGGAGCAAGGAAGGATTGAGGGTCTCGTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCGCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                           <  2:202464/297‑1 (MQ=255)                                                                                                                                                                                                                                 cgtcggcacgcgtagaaacatGCCACAACA‑AAACAGGCAAATCTGACCGGTACATCAATCGAGCAGGGTAAAGGTCGCCATCGTCTGGAACTGTTTCTGGAAGTAATGAACGCTTGTGGATCGCTTGAATGGACTCGGCAGCGTGCCGAGGAAGAAGCAGACAAAGCCAGCGCAGCGTTACAGGTTCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTTAACGCGATCGTTAATCCCCGCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                           <  2:236287/280‑1 (MQ=255)                                                                                                                                                                                                                                   gctgcATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTCCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCACCGCCCTCCCGCACATCGCTGTTCAACCCCACCGTTAATCCCCCCCCCCCACCCCCCCCACCCCGCGGGAGGGGTCCCGTAAGtt                                                                       >  1:372694/1‑300 (MQ=255)                                                                                                                                                                                                                                    cggaaTGCGATGCAGAATGGCA‑ACCACGAACAGGCACAGATGAGCCGTGCCGCCATCGAACAGGGTAACGGTCGCCCTCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGGGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTc                                                                      <  1:432712/296‑1 (MQ=255)                                                                                                                                                                                                                                          gCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTGCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACACAGCAATCGCAGCGTTACAGGTGCTCCAGGACACCCCTTGGCGAGAAGCACTCATCGGACTGGCGCACACAGGTGGTCAACGGGATGTTTAATCGCCTCCCCCCATCCCGCCCATCCAGCGGGAGGGTTcacggcaagtccatcaaa                                                               >  2:77695/1‑284 (MQ=255)                                                                                                                                                                                                                                                    atGGAACACAA‑GAACAGGCAAATGTGAACCGGCACGCCATCTAACTGGGTAACGTTCGCCAGCTGCTGGAACCGGTTCTGGCAGCAATGAACGCTTGTGGTTCTCTTGCATGGACGACTCAGCGTGCCGAGGCAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCTCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGGTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc                                                     <  2:215431/301‑1 (MQ=255)                                                                                                                                                                                                                                                      ggCACACCA‑GAACAGGCACAGAGGATCCGTACCGCCATCGAACAGGGGAACGGTCGCCATCGTCTGGAACCGGTTCGTGACGCAATGAACGCTTGTGGATCTCGTGAAGGTGCGCGTAAGCGTGACGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGTTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc                                                     <  2:168514/299‑1 (MQ=255)                                                                                                                                                                                                                                                          cacCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGCAGCAATGAACGCTTGTGGATCTCTTGAATGGCCGCGTCAGCGTGCTGAGGAAGAAGCAGACAAAGCCATCGCAGCGATACAGGTGCTCCGGGACACCCCTTGGCGAGAAGCACTCATCGCACTCGCGCCCATCTCTGTGCAACGCGATCGTTAATCCCCTCCCCTCATcc                                                                                                      >  2:406808/1‑246 (MQ=255)                                                                                                                                                                                                                                                          cacCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACCAAGCCATCGCAGCGTTACAGGTGCTCCCGGACAGCCCTTGGCGAGAAGCACGCATCGGCCTCGCGCACATCGCTGTTCCACACGCTCGTTAATCCCCTCCCCTCATCACGCGCAGCCGACAGGACGGGTCCAGTACTGTCCCTCAACACACCTATTccactca                                               >  2:178845/1‑295 (MQ=255)                                                                                                                                                                                                                                                            ccA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCAATCCCGCGCAGCGCGCGGGCTGGTTCCCGTACGTTCCATAAAAACACTTATTCAGCCCtat                                             >  2:183721/1‑301 (MQ=255)                                                                                                                                                                                                                                                                 aaCAGGCACAGATGACCCGTACCGCCAGCGAACAGGGTAACGGTCGCCATCTTCGGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATaa                                           <  1:178844/299‑1 (MQ=255)                                                                                                                                                                                                                                                                 aaCAGGCACAGAGGATCCGTACCGGCAGCGAACATGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATaa                                           <  2:343510/299‑1 (MQ=255)                                                                                                                                                                                                                                                                      gaacaGATGTGCCGTACGTCCATCAAACAGGGTAACGGTCGCCATCGTCTTGAACCGGTCCTGGAAGCAATGAACGATTGTGGATATCGTGAAGTGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAac                                          <  2:394905/293‑1 (MQ=255)                                                                                                                                                                                                                                                                         cagatacgccGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGCAGCAATCAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCCTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAac                                          <  2:19202/284‑1 (MQ=255)                                                                                                                                                                                                                                                                         caGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCCTCGGCCTCGCGCACATAGCTGTTCAACGCGATCGTTAATGCCCCCCCCTCATCCCGCACAGCGCGCGGACTGGTTACAGTAAGCTCCATCAAAACACTTATTCCGctctctcac                                          >  1:19202/1‑287 (MQ=255)                                                                                                                                                                                                                                                                                               aaaaGGGTAACGGTCGCCAGCTTCTGGAAACGGTTCTGGAAGAAATGAACGATTGTGGATCTCTGGAATGGACGCGGCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCTCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat           <  2:149336/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        aaggTCGCCAGCTTCTGGAACCGGGTGTGGAAGCAAGGAACGCTTGTGGAGCTCGTGAATGGACGCGGCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAat  <  2:221227/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                        GCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCA‑GAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAAT  >  NC_000913/3334203‑3334797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.