Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,706	T→G	K173Q (AAA→CAA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,706	0	T	G	100.0%	105.9 / NA	31	K173Q (AAA→CAA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/15);  total (16/15)

AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGC  >  NC_000913/4116410‑4116994                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                 aaaGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGAACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAg                                                                                                                                                                                                                                                                                              <  1:59091/301‑1 (MQ=255)            tGCCATAGGTGTTCTTCGCCATCCCTTCTTTCCCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACGTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACg                                                                                                                                                                                                                                                                                    <  1:36919/300‑1 (MQ=255)                                   ccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCCCGTATCAACCGTACCAAACAGCAAtt                                                                                                                                                                                                                                                            >  1:465815/1‑301 (MQ=255)                                                 caACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCa                                                                                                                                                                                                                                              <  2:41854/301‑1 (MQ=255)                                                        ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTcg                                                                                                                                                                                                                                        >  2:344194/1‑300 (MQ=255)                                                        ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTcg                                                                                                                                                                                                                                        >  2:121759/1‑300 (MQ=255)                                                          aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCga                                                                                                                                                                                                                                     >  2:413689/1‑301 (MQ=255)                                                           aaCAGTGAGGCCTCCGGGTCACAGGCAATCCCGGAGATTGGAATACGCGTGCGGCCTTTGCCGCCAATGTAAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgag                                                                                                                                                                                                                                    <  1:121608/301‑1 (MQ=255)                                                              aGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCaagataggaagagcacacgtct                                                                                                                                                                                                                                  >  2:311592/1‑282 (MQ=255)                                                                   ggCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAAGAGAGGCGTTGGTGTAATCGGTCACATGGACAAGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCCCGACGTGCAAGCTCGCGAGAGCCTTc                                                                                                                                                                                                                             >  2:239557/1‑300 (MQ=255)                                                                              cACCGTGGATCCCGGCGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                                                                                                                                                                                 <  1:365020/301‑1 (MQ=255)                                                                                            gagaTTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcact                                                                                                                                                                                                    <  2:466404/300‑1 (MQ=255)                                                                                                     cctacacgacgcgcttccgatctaaTGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAg                                                                                                                                                                                          <  1:271996/278‑1 (MQ=255)                                                                                                      ttcagacgtgtgctcttccgatcttGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAg                                                                                                                                                                                          <  2:136999/276‑1 (MQ=255)                                                                                                                  cTTTGCCGCAAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                                                                                                                                                                             <  1:343758/301‑1 (MQ=255)                                                                                                                        cgccAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAc                                                                                                                                                                       <  2:44383/301‑1 (MQ=255)                                                                                                                            aaTGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGagatcggaagagcacacgtctga                                                                                                                                                                   >  2:272317/1‑279 (MQ=255)                                                                                                                              tGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCCCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGagatcggaagagcgtcgtgtagggg                                                                                                                                                                 >  1:136830/1‑277 (MQ=255)                                                                                                                                       gACCGTATCATGCGGAAAAACGACGCACTTCTGGCAGCATCTCTGCAGGAAAAGCCAGCACTTCAATAATGTTTTCGGCCCAGTCCAGGGTATGGATGTTGAAGAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc                                                                                                                                                         <  1:239271/300‑1 (MQ=255)                                                                                                                                                    ggAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTTAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCCCGACGTGCACTCTCGCGAGAGCCTTCCACATTGTCGAGGATCCACTTCACTTTTGTGCCAGAAACGTCCGGGTCAATCACCATACCGGTATTGCTGCGGAtatact                                                                                                                                             >  1:234439/1‑297 (MQ=255)                                                                                                                                                                     cTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATCGTCGAGGGTCCACTTCACTTTGGGGCCAGAAAAGTACGGGTCAATCACCAGACCGGCATTGCTGCGGATATACTCTTCTCAACCGTAAcagtt                                                                                                                          >  1:123101/1‑297 (MQ=255)                                                                                                                                                                                    tcgtaaTATCTATCACTTCCAGCAGTCTGTAGTCCCAGACCAGGGTTTTTATGTTGAACAACATAGTACGCGAGTCGTTGGTGGAATAGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCAAGTATCAACCGGAACAAACAGCACTTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCGCTTTGGTGCCCGAAAAGTACGGGTCAATCACCAGACCTGTAGTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTCAATGCTCGCAGa                                                                                                            <  1:413152/296‑1 (MQ=255)                                                                                                                                                                                          ttccctacacgacgctcttccgatcttcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGc                                                                                                     <  1:412006/275‑1 (MQ=255)                                                                                                                                                                                                                    tcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGcagatcggaagagcacacgtctgaa                                                                             >  2:412543/1‑275 (MQ=255)                                                                                                                                                                                                                         gTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTta                                                                      >  2:298256/1‑301 (MQ=255)                                                                                                                                                                                                                                         ttGAACAAAATGGTACGGGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCgg                                                      <  1:297895/301‑1 (MQ=255)                                                                                                                                                                                                                                          tGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCggg                                                     >  2:229840/1‑300 (MQ=255)                                                                                                                                                                                                                                              caacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCCAATGGCGTTATAGATAGGCTTGCCGGttt                                                   >  2:278625/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                   tGGACACGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTaa            >  2:41719/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                      acacGGCCCTGAGTCATTTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATa          >  2:219867/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ccTGAGTCATCTGCCCGATCAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGc  <  1:41668/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                 AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGC  >  NC_000913/4116410‑4116994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑