Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,170,457	0	A→C	100.0%	1.1	3	K74N (AAA→AAC)	insE1	IS3 transposase A
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (1/0):  total (3/0)

GAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAACGTGAACTGGAGATGTCTACCGAGATTGCACGTCTCAAACGCCAGCTGG  >  NC_000913/2170160‑2170467                                                                                                                                                                                                                                                                                                          |           gaATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAACCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCGGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAACGTAAACTGGAGATGTCTAACGAGATTGCACGTCTCAAACg          >  1:527705/1‑300 (MQ=17)        ggTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTACACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCCGAACGCATCGGTGTCACTGCCGCAGCCCGTGAACTGAACCTGTATGAATCTCAACTCTACATCTGGCGCAGTACACAGCCAATTGAGCAGTACTCTTCTGAACTTGAACTGGGGATTTCTACCGAGATTGCCCGTCTCAACCGACactatg  >  1:569737/1‑296 (MQ=11)        ggTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACCACTCCACAACTGGCGCAGTAAACAGCAAAATCAGAAGACGTCTTCTGAACGTGAACTGGAGATGTCTACCGAGATTGCACGTCTCAACCGCCCGCTgg  >  1:519937/1‑301 (MQ=11)                                                                                                                                                                                                                                                                                                          |           GAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAACGTGAACTGGAGATGTCTACCGAGATTGCACGTCTCAAACGCCAGCTGG  >  NC_000913/2170160‑2170467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.