Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,797,027	0	T→G	100.0%	0.9	1	I27R (ATA→AGA)	waaL	O‑antigen ligase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/1):  ref base (0/0):  total (0/1)

GCTGGCCGGGGCTAAATTTGTAGTGTCGGTGGATACGGGGTTAAGCCATTTAACGGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCAACCGATCCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGAGAAAATTTAATTAACCTCAACAGTCAAGCAGTTTTGGAAAAGTTATCATCATTATAAAGGTAAAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTT  >  NC_000913/3796728‑3797106                                                                                                                                                                                                                                                                                                            |                                                                                gCTGGGCGGGGCGAAATTTGTAGTGGCGGTGGATACGGGGTTAAGCCATTTAACGGCGGCAATGGATAGACCCAATATCACGGTTTATGGAACAACCGATCCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGAGAAAATTTAATTAACCTCAACAGTCAAGCAGTTTTGGAAAAGTTATCATCATTATAAAGGTAAAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTtatat                                                                                 <  2:722477/300‑1 (MQ=255)  cTGGCCGGGGCTAAATTTGTAGTGTCGGTGGATACGGGGTTAAGCCATTTAACGGCGGCACTGGATAGACCCAATATCACGGGTTATGGACCAACCGATCCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGAGAAAATTTAATTAACCTCAACAGTCAAGCAGTTTTGGAAAAGTTATCATCATTATAAAGGTAAAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTatatat                                                                               <  1:440889/301‑1 (MQ=255)   tGGCCGGGGCTAAATTTGTAGTGGCGGTGGAGACGGGGTTAAGCCATTTAACGGCGGCACTGGATAGACCCAATATCACGGTTTAGGGACCAACCGATCCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGAGAAAATTTAATTAACCTCAACAGTCAAGCAGTTTTGGAAAAGTTATCATCATTATAAAGGTAAAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTatatat                                                                               <  1:821005/300‑1 (MQ=255)                                                                               cACGGTTTATGGACCAACCGATCCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGAGAAAATTTAATTAACCTCAACAGTCAAGCAGTTTTGGAAAAGTTATCATCATTATAAAGGTAAAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATAGATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGtt  <  2:1053844/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                GCTGGCCGGGGCTAAATTTGTAGTGTCGGTGGATACGGGGTTAAGCCATTTAACGGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCAACCGATCCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGAGAAAATTTAATTAACCTCAACAGTCAAGCAGTTTTGGAAAAGTTATCATCATTATAAAGGTAAAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTT  >  NC_000913/3796728‑3797106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.