Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,558,995 Δ1,040 bp [rtcR][glpR] [rtcR], [glpR]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3558995 3560034 1040 43 [0] [0] 45 [rtcR]–[glpR] [rtcR], [glpR]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 35589940 (0.000)41 (0.920) 39/578 0.2 100% coding (727/1599 nt) rtcR sigma 54‑dependent transcriptional regulator of rtcBA expression
?NC_000913 3560035 = 0 (0.000)pseudogene (419/606 nt) glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

GCCGGACGCCAGCTTTTTGACGTTTCGCGCCAGGGCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT  >  NC_000913/3559735‑3560335
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                            
gcaaaaaagaacagccgcgcggcccaggtgaagtaacgattatcgatctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttcgggcgcgccgcatcttaaagttaaaaccggcgcggcatcagtttagcggctcgtttgg                                                                                                                                                                                                                                                                                                              >  1:412328‑M2/301‑301 (MQ=255)
 caaaaaagaacagccgcgcggcccaggtgaagtaacgattatcgatctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttacgtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaaggggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccgccccgggcaagtcatttatggaccgacgcatcttagagttacaacaggcgcggcacaaggttagcggcccgttttGt                                                                                                                                                                                                                                                                                                             >  1:227080‑M2/300‑301 (MQ=255)
    aaaagaacagccgcgcggcccaggtgaagtaacgattatcgatctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgccgcatcttagagttaaaaaaggcgcggcatcagtttagcggcgcgtgtgGTGCg                                                                                                                                                                                                                                                                                                          >  2:238276‑M2/297‑301 (MQ=255)
              ccgcgcggcccaggtgaagtaacgattatcgatctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGt                                                                                                                                                                                                                                                                                                  <  2:85014‑M2/13‑1 (MQ=255)
                gcgcggcccaggtgaagtaacgattatcgatctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgcatgagcagatcgaaaaagtgtcgagcaaatcccgcgcgccgattctgcttaacggtccaatcggcgcgggcaagtcatttctggagcgacgcatcttcgagttaaaacagtcgcggcatcagtttagctgcgcgtttgGTCCGCACTTCGTGGTa                                                                                                                                                                                                                                                                                              >  1:199829‑M2/285‑301 (MQ=255)
                      cccaggtgaagtaacgattatcgatctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGaa                                                                                                                                                                                                                                                                                        >  1:41805‑M2/279‑301 (MQ=255)
                             aaagtaacgatgaacactctcgatttaagcccgatacacccccacgccacccgctgtgccgaggaacgcccgcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacgtcaaccgcatgattgggcagatcgaaaaagtggcgatcaaaacccgcgcgcccattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCcagc                                                                                                                                                                                                                                                                                 <  1:238040‑M2/30‑1 (MQ=255)
                                           cgatctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGc                                                                                                                                                                                                                                                                    >  2:50884‑M2/258‑300 (MQ=255)
                                             atctcgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgccgcatcttagagttaaaacaggcgcggtatcagtttagcggcgcgtttgGTTCGAACTTCGTGGAAATCGAAATCCAGCAGCGAGCCGTCGCAAt                                                                                                                                                                                                                                                                 >  2:182593‑M2/256‑301 (MQ=255)
                                             atatcgatttaagcggttataacgccctggccagccgcttggccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacgtcaaccgcaggattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTAt                                                                                                                                                                                                                                                                 <  1:449597‑M2/46‑1 (MQ=255)
                                                 cgatttaagccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAACCGTCGCtatcaag                                                                                                                                                                                                                                                             >  2:5404‑M2/252‑298 (MQ=255)
                                                         gccgttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTa                                                                                                                                                                                                                                                     >  2:134436‑M2/244‑301 (MQ=255)
                                                             ttataacgccatcgccagccgctttgccgaggaacgccagcaaacgcttgattttcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATcc                                                                                                                                                                                                                                                  <  2:41844‑M2/61‑1 (MQ=255)
                                                                                                              gattttcttaagtccgtcatagccacgcgtaacccctacttcaatcgtaggattgagaagatcgaaaaagtggcgattaaagcccgcgcgccgattctgctgaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggtatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGc                                                                                                                                                                                                 <  1:182398‑M2/110‑1 (MQ=255)
                                                                                                                   tcttaagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCAAGCGTCACCTCGc                                                                                                                                                                                            >  1:397200‑M2/186‑300 (MQ=255)
                                                                                                                   ccttaaggccggcatcgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGcc                                                                                                                                                                                           <  2:397681‑M2/116‑1 (MQ=255)
                                                                                                                      taagtccggcattgccacgcgtaacccccacttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAAt                                                                                                                                                                                        <  2:294552‑M2/119‑1 (MQ=255)
                                                                                                                                                   ccttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGc                                                                                                                                                           <  2:379587‑M2/148‑1 (MQ=255)
                                                                                                                                                   acttcaaccgcatgattgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCg                                                                                                                                                            >  2:336691‑M2/154‑300 (MQ=255)
                                                                                                                                                    acgcccttccgatcttgcgaagatcgaaaacggggcgatcaaatcccgcgcggcgattctgcttaacggtccaaccggcgcgggcaagtcattgctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGcc                                                                                                                                                          <  1:289422‑M2/149‑1 (MQ=255)
                                                                                                                                                                   tgagcagatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCtgcgtaattcgccagatcggaagagca                                                                                                                                            >  2:289747‑M2/138‑287 (MQ=255)
                                                                                                                                                                       gcagatcgaaaagtggcgatcaaatcccgcgcgctgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgattgGTGCGAACTTCGTGGTACTCGAACTCCAGCAGCGAGCCGTCGCCAGCGTTGTCGCCTATCCCCAGACAGCCGAACTCAAGGCGGAACTGGGAGACCAAATCGAACCTCGCTTGGCCAATGACGCCGCCATCCCGGATGCGTGATTCTCCCCCGGccagcatcattgca                                                                                                                                       >  1:137950‑M2/134‑297 (MQ=255)
                                                                                                                                                                        agatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGaa                                                                                                                                      >  1:327111‑M2/133‑301 (MQ=255)
                                                                                                                                                                         gatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGaaa                                                                                                                                     >  2:236063‑M2/132‑301 (MQ=255)
                                                                                                                                                                         gatcgaaaaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGaaa                                                                                                                                     >  2:196589‑M2/132‑301 (MQ=255)
                                                                                                                                                                                aaagtggcgatcaaatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAAtcttct                                                                                                                              <  2:227316‑M2/177‑1 (MQ=255)
                                                                                                                                                                                   ggggcgatcaaatcccgcgcgcagatactgcttaacggtccaaccggcgcgggcaaggcatttctggcgcgacgcatcttagagtgaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCttt                                                                                                                            <  1:196381‑M2/179‑1 (MQ=255)
                                                                                                                                                                                     ggcgacaaaatcccgggcgccgatgctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtgtagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTa                                                                                                                          <  1:235827‑M2/181‑1 (MQ=255)
                                                                                                                                                                                             aatcccgcgcgccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACg                                                                                                                 >  2:34334‑M2/112‑301 (MQ=255)
                                                                                                                                                                                                    cgggccgattctgcttaacggtccaaccggcgcgggcaagtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAg                                                                                                           <  1:336289‑M2/196‑1 (MQ=255)
                                                                                                                                                                                                                     acggtccaaccggcgcgggcaagtcatttctggcgcgtcgcaccttagagttaaaacaggcgctgcatcagttcagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTgg                                                                                         >  2:150908‑M2/88‑301 (MQ=255)
                                                                                                                                                                                                                                         aagtcatttctggcgcgccgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACGCCAGCAGCGAGCCGTTGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAAGTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCtg                                                                     <  2:138092‑M2/234‑1 (MQ=255)
                                                                                                                                                                                                                                          agtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTg                                                                        >  1:164984‑M2/67‑297 (MQ=255)
                                                                                                                                                                                                                                           gtcatttctggcgcgacgcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGTCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCtgtg                                                                   >  2:100819‑M2/66‑301 (MQ=255)
                                                                                                                                                                                                                                                          aagcatcttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCGTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAgtgcgtg                                                    <  1:134294‑M2/251‑1 (MQ=255)
                                                                                                                                                                                                                                                                cttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCATTGCGTCCGCTa                                               >  1:337448‑M2/45‑300 (MQ=255)
                                                                                                                                                                                                                                                                 ttagagttaaaacaggcgcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTAc                                              >  2:266935‑M2/44‑300 (MQ=255)
                                                                                                                                                                                                                                                                                  gcggcatcagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACACTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGGGGTGc                             >  2:108786‑M2/27‑300 (MQ=255)
                                                                                                                                                                                                                                                                                   cggcatctggttagtggggcgtttgGTGCGAACTTCGTGTTAATCGAACACCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAAAGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGGTCCCGCCAGCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTATTTTACCATCAACATGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAGATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGcc                            <  1:150750‑M2/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        ccgctcttccgatctgtttgGTGCGAACTTCGTGGTAAGCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATc                      <  1:387028‑M2/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         cagtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCg                     >  2:91633‑M2/20‑301 (MQ=255)
                                                                                                                                                                                                                                                                                          agtttagcggcgcgtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCATTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGCTATcgt                    >  2:83137‑M2/19‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                       gtttgGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATTCGCAAATTGCTGTGATTGAGCAGTTGGTGCGCTACCGTTTCCGgcgtggtgacgatatcagatccgaagagcaa       >  2:387491‑M2/6‑286 (MQ=255)
                                                                                                                                                                                                                                                                                                            gTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGCAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAt  >  1:340658/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                            gTGCGAACTTCGTGGTAAACGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCa   <  1:266640/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                            
GCCGGACGCCAGCTTTTTGACGTTTCGCGCCAGGGCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT  >  NC_000913/3559735‑3560335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: