New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1096961 =	31 (0.800)	7 (0.250)	3/438	4.2	24.3%	intergenic (+132/+604)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
	?	NC_000913	= 1097503	21 (0.740)					intergenic (+674/+62)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
Rejected: Coverage evenness skew score above cutoff.

GGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTT  >  NC_000913/1097215‑1097788                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               gcggAGTGACCCGTTACGCGCGCGATGCGGGCAGACAGCGCCATGCGTTCAAGGTCTGTCGAACTGGCTGTGGCCAGGTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGtgtgtg                                                                                                                                                                                                                                                                                     <  1:216792/298‑1 (MQ=11)         aCTCGCTTCGCTCGCCCTGCGGGCATCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATAc                                                                                                                                                                                                                                                                             <  2:156753/300‑1 (MQ=14)                        ccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCCTCCCTCTCCCTGCGGGCAGCCCGCCCACGGCGTTCACGGTCTGTCCCACTGGCTGTCGCCCGTTTTCGAACCACCGTCAGGGCCTCTCATCCCCCCGGTGTGTGCCATCTACGCAACAATAGCACGTa                                                                                                                                                                                                                                                             >  1:243999/1‑301 (MQ=11)                                                 tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGGTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCGTCTCATCCCCCCGGTGTGTGCAATCTACTAACAAAAAGCCAGTACTTTCGTACGAACacttacttacat                                                                                                                                                                                                                                    >  1:323057/1‑293 (MQ=11)                                                         tGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCGCACTGCCGTCACGGTCTGTCCAACTGGCTGTCTCCAGTTGTCGAACCCCGGTCGGGGCTTCCCATTCCCCCGGGTTGTGCAACTTACGAAAAAAAAGCCCGTACGTTCGTACGATCGCTTCTTGAACTATGGCggg                                                                                                                                                                                                                            >  1:372658/1‑300 (MQ=11)                                                               aCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTTGCCCGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTTTGCAATATACGCAAAAAAAGCCCTTTCGTTCGGACGAGCCCTTCCTTACATATCGCGGTGAggag                                                                                                                                                                                                                      >  2:66761/1‑299 (MQ=11)                                                                      gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGAAACCCTTCGCTCGCCCTCCGGGCAGCGCGGTCACTGCGTTCAGGGTCTGTCCAACTGGCTGGCGCCCGTTGTCGAATCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTCATTTCGTACTAGATCTTCTTTACATATTGCGTGGAGGGGGGGAttt                                                                                                                                                                                                               >  1:102669/1‑300 (MQ=11)                                                                       tCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGACAAAAAAGCCCGTACTTTCGTACGAACTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCTCCCTGCTGGCAGCCCGCCCACTGCGTTCACGGTCTGTCCAACTGGCTGTCTCCTGTTGTCGATCCCCGGTCGGGGCTTCTCATCCCCCCGGTGGCTGCAATATACGAAAAAAAAGCCCGTACTATTGACCTATCTCTTCTGTGAATATTGCCGTGAGGGGGGGTTTca                                                                                                                                                                                                              >  2:137932/1‑300 (MQ=11)                                                                        cGCCGGTTGCCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGGTTGAAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGa                                                                                                                                                                                                             <  2:162423/301‑1 (MQ=17)                                                                                    aaCCCCCGGCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGG‑‑TTCGAAtccccccctcac                                                                                                                                                                                                <  2:244257/300‑8 (MQ=2)                                                                                            tCGGGCCTTCTCCTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGt                                                                                                                                                                                          <  2:102782/300‑1 (MQ=255)                                                                                                                        cAATCTACGAAAAAAAAGCCCGTTCTTGCGTACGAGCTGTTCTTTGAATATGGCGTTGAGGGGGGTATTGACTCGCTTCGCTCGCCCGGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCa                                                                                                                                                              <  2:117377/300‑1 (MQ=255)                                                                                                                                     aaaaGCCCTTTCTTTCGTACGAGCTTTTCTTTAAAGATGGCGGTGAGGTGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACAcc                                                                                                                                               <  2:170163/301‑1 (MQ=255)                                                                                                                                                              cttcttTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGACGTATACACACTTTCCAGGCGTGCTCCTTCCGCCCCT‑CGGCCACGCCCCCAAACTATCGGTCCTGTCTTg                                                                                                                      >  1:122642/1‑301 (MQ=255)                                                                                                                                                                   ttAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGa                                                                                                                 >  2:377879/1‑301 (MQ=255)                                                                                                                                                                                             gACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATCCGGTCCCGTATAAACACTTTCCAGGCGTGCTCCCTCCACCACTCCGGACCCCTC‑CCAAAATTTCTATCCTGTCTTTCTGGAACTTGCGCTAATTTAGGGatactctt                                                                                       >  1:239768/1‑294 (MQ=17)                                                                                                                                                                                                        ctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTca                                                                            <  2:57283/301‑1 (MQ=255)                                                                                                                                                                                                          cgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCCCCAAATTTTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATgctcgtcggtcaaa                                                                          >  1:57226/1‑288 (MQ=255)                                                                                                                                                                                                           tcagacgtgtgctcttccgatcTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca                                                                         <  2:31513/280‑1 (MQ=255)                                                                                                                                                                                                                        cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa                                                            <  2:122775/301‑1 (MQ=255)                                                                                                                                                                                                                                  gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTTCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaacaagatcggaagagcgtcgtgtagg                                                  >  1:31481/1‑279 (MQ=255)                                                                                                                                                                                                                                                tCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTtca                                    <  2:176520/301‑1 (MQ=255)                                                                                                                                                                                                                                                             cGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTCTACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTTTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGt                       >  2:371307/1‑301 (MQ=255)                                                                                                                                                                                                                                                                   ttGCCGAACCCCGGTCGGGGCTTCTCATCCGCCCGGTGTGTGCAATATACGAAGAAAAAGCCCTTACTGTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTtaat                 <  1:377418/301‑1 (MQ=255)                                                                                                                                                                                                                                                                         aaCCCCGGTCGGTGCTTCTGATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCg            <  2:331369/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         aaCCCCGGTCGGGGTGTCTCATCGCCCCGGTGTGTGCAAGATACTAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCg            <  1:370846/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCCCTCATTTAGGGAAATCCTGACCTGAGGGCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTTGTTTTAATCAGCGaaa         >  1:312394/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                  cGGGGCTCCTCATCACCCCAGGGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGATCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTTGAACCCCCGATACGCTGCCGATTACACCCTTTCCAGGCGTGCTCCTTCAGCCACT‑CCGACACCTCACCAAATTATCGTTCCTGCCTTTCTGGTAAGGGAGCTAATTTAGGGAAATCATTACCTAAGGGCCACAGAACTGTTGAAGAAATGACTGATTTATTAAAACATCAATCACTGTTTGGTTTTAATAATTGATATcttctt  >  1:124945/1‑301 (MQ=18)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               GGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTT  >  NC_000913/1097215‑1097788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.