Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,407	C→A	M272I (ATG→ATT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,407	0	C	A	100.0%	145.0 / NA	41	M272I (ATG→ATT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/23);  total (18/23)

ATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGA  >  NC_000913/4116129‑4116698                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                    aTCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAAGGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAgg                                                                                                                                                                                                                                                                               <  2:266290/301‑1 (MQ=255) aTCGCCCCACGCGAATACGGGTCCCGGTACGGCGCAACCAGCCCGGTAAATGCCGGAACCAAATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAgg                                                                                                                                                                                                                                                                               <  2:118161/301‑1 (MQ=255)           tgtgctcttccgatctgTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGc                                                                                                                                                                                                                                                                     <  2:219531/285‑1 (MQ=255)            cgcATGGGGGGCCCAGCAGGGCGGACCCAGCGCGGTGAAGGCCGGAGCCACGTACAGCCCATGGGTGTTGTGCACGTTGGTGGGGAAAGAGTCGGAATCGTAGGCGTCGTTAAGCAACTGCATTTCGGCGCGCAGCCACTGAATGGATGCGCGTGCCATAGACACCGCACCGTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGc                                                                                                                                                                                                                                                                     <  1:329903/300‑1 (MQ=255)             gcATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCa                                                                                                                                                                                                                                                                   >  2:269696/1‑301 (MQ=255)                        ccAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCttt                                                                                                                                                                                                                                                         >  2:84455/1‑300 (MQ=255)                          accaCGGCGCACCCAGCCCGGTAAAAGCCGGAACCACAGACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCAc                                                                                                                                                                                                                                                      <  1:62396/298‑1 (MQ=255)                           gTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCCTAGGTGTTCTTCGcagatcgtaagagcgcg                                                                                                                                                                                                                                                     >  1:219312/1‑285 (MQ=255)                              cGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCAcagat                                                                                                                                                                                                                                                  >  2:62467/1‑297 (MQ=255)                                    ccccAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTTAAAGTGGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAAGGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGa                                                                                                                                                                                                                                            <  1:269398/300‑1 (MQ=255)                                          cccGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAAc                                                                                                                                                                                                                                      <  2:194377/301‑1 (MQ=255)                                                aaaTGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCCCAACTGACCaacccgcccg                                                                                                                                                                                                                                >  1:196798/1‑293 (MQ=255)                                                         aaCCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTgg                                                                                                                                                                                                                       <  2:11583/301‑1 (MQ=255)                                                         aaCCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTgg                                                                                                                                                                                                                       <  2:102843/301‑1 (MQ=255)                                                             acaTACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCAc                                                                                                                                                                                                                   <  2:351905/301‑1 (MQ=255)                                                                    cacCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGAt                                                                                                                                                                                                            >  2:224192/1‑301 (MQ=255)                                                                           ggTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGgag                                                                                                                                                                                                     >  2:102261/1‑301 (MQ=255)                                                                                        tggggggCGAAATATTCGGAAGCGGAGGCGTCGGTAATCAACTTCAGTTCATCGCGCAGCCACGGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCGCTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCTTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgcg                                                                                                                                                                                        <  1:102148/296‑1 (MQ=255)                                                                                                              cGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGtt                                                                                                                                                                  <  2:196995/301‑1 (MQ=255)                                                                                                               gTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCCAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGGCGGCCGCCTTTGCCGCCTATGttt                                                                                                                                                                 >  1:69664/1‑300 (MQ=255)                                                                                                                    ctacgtTAATCAACTCCAGTTCATCGCGCAGCCCCTGAATGAATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCGTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTg                                                                                                                                                            <  1:290626/298‑1 (MQ=255)                                                                                                                       cgtTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc                                                                                                                                                          >  2:60177/1‑300 (MQ=255)                                                                                                                                    catttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACACATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaa                                                                                                                                            <  2:369642/301‑1 (MQ=255)                                                                                                                                       ttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaagaa                                                                                                                                         >  2:229012/1‑301 (MQ=255)                                                                                                                                        tcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaagaa                                                                                                                                         >  2:371107/1‑300 (MQ=255)                                                                                                                                        cccagagcacaaaacggcatgagtGCGCCTGCAAACAAATCCGCACCATCCAACGCATAGGTCACTTCGCCAGTCGGGCCGCCGGAGATGGTGATCAGCAGGCCGTTTTCTGATTTCACCGCTTTCGCGCCATTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTAGCCATCCCTTCTTTCGCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAac                                                                                                                                        <  1:169419/278‑1 (MQ=255)                                                                                                                                             gcgcAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCACTGTTAGTCTGACCGTATACTTCGGAAGAacgacg                                                                                                                                    >  2:23035/1‑300 (MQ=255)                                                                                                                                                        gAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGgcag                                                                                                                        >  2:195205/1‑301 (MQ=255)                                                                                                                                                             gATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTCCCGCCAATGTTAGCCTGACGGTATACTTCGGAAGAACGACGCACTTCTGgcagctcc                                                                                                                    >  1:168418/1‑297 (MQ=255)                                                                                                                                                                                  ccACCTTCCAACGCATAGTTCACTGCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACtt                                                                                              <  2:267611/300‑1 (MQ=255)                                                                                                                                                                                       ttCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGc                                                                                         <  2:69745/301‑1 (MQ=255)                                                                                                                                                                                                     tCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTGCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCa                                                                           <  1:329208/301‑1 (MQ=255)                                                                                                                                                                                                                      ccGCAGGCGATGGTGGGCAGCAGGCCGGTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTGCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGt                                                          <  1:223959/301‑1 (MQ=255)                                                                                                                                                                                                                           ggCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATACCCAGCACTTCCAGCATTTTGTCGTCCCAGTCACGGGTATGGATGTTGaaa                                                     >  1:337464/1‑300 (MQ=255)                                                                                                                                                                                                                           ggCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTGGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGACCGTCGCACTTCTGGCACCATCTCGAGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaaa                                                     >  1:192713/1‑300 (MQ=255)                                                                                                                                                                                                                               atgttcgtCAGCACGCCGTGTTCTGAGTTCACCGCGTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAacaaca                                                 <  1:228779/295‑1 (MQ=255)                                                                                                                                                                                                                                             gCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATAT‑CAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACCTGGTACGAGAGGCGt                                  >  1:106241/1‑301 (MQ=255)                                                                                                                                                                                                                                                   ttCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGACAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgtg                             <  1:370646/301‑1 (MQ=255)                                                                                                                                                                                                                                                                gCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGTGGCGTTGGTGTAATCGGTCACAt                >  2:168921/1‑301 (MQ=255)                                                                                                                                                                                                                                                                             tgtTCATCAGAATAAAGCAGCCCGTGCCATTGGTGTTCTGCGCCATCCCTTCTTTCACGCACAACGTACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACTCGTGCCGCCTTTGCCGCCAATGTTAGTCGGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTg   <  1:168748/301‑1 (MQ=255)                                                                                                                                                                                                                                                                             tgtTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATAT‑CAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGa  <  2:106354/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                    ATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGA  >  NC_000913/4116129‑4116698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑