Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,004,6160AC45.0% 18.0 / 44.5 40T249P (ACC→CCC) fliDflagellar filament capping protein
Reads supporting (aligned to +/- strand):  ref base A (8/14);  new base C (11/7);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCAGACGCAGTTGAAATCGATGCTGAGTAATACCGTCAGTTCTTCCAGCTATAAAACGTTGGCGCAGA  >  NC_000913/2004330‑2004901
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                             
ccGAGATGCCAGAAACAACGCAAAAGAAGGAGAAAGCGCAAGCATCAGTGACGTGGGTAAAGGTGGATAGCGTCTGTCAGTCACATCAAAGGACACCGGCCTTGATAATGCGATGACACTTTCGGTCAGCGGGCATGATGGGCTAGAAAGTTGTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCAGCCCAGAATGCGCAGCTGTCAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCctgaacctgaac                                                                                                                                                                                                                                                                                 <  1:46461/301‑1 (MQ=255)
       gCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATAGAGAACAGAAGCAACAACATCAGCGACGCGCTGGAAAACATCCCcctgaacctgacagctgtc                                                                                                                                                                                                                                                                           >  2:273757/1‑292 (MQ=255)
        ccATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTca                                                                                                                                                                                                                                                                          >  2:136639/1‑300 (MQ=255)
                  cGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAAAGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGACCGATGTCACCAAGGGCaa                                                                                                                                                                                                                                                                >  1:49449/1‑300 (MQ=255)
                    cAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCCACGGTATGGAGGTCTCGGTTGTCGCCAAGTATGCGCAGCTGACAGTAAACAACGACAGCATCGAGAACAGCAACAACACCAACGGAGAGGCTCTGGCAACCATAACacctaaccagaaacgaggtagacagggcaacca                                                                                                                                                                                                                                                             >  1:409941/1‑280 (MQ=255)
                            ggCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCAACAGCGACGCGCGTGAAAACATCCCCCTGAACCCGAACGCTGTCACCACGGGCAACCAGACGCaaa                                                                                                                                                                                                                                                     >  1:60891/1‑301 (MQ=255)
                                    cgaaaGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCAc                                                                                                                                                                                                                                              <  2:313242/297‑1 (MQ=255)
                                     gcAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCATCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAAAACAGCAGCAACACCATCAGCGAGGCGCTGGAAAACATCCCCCTGAACCTGCACGATGTCACCACGGGCAACCAGACGCTAACCATCACt                                                                                                                                                                                                                                             >  2:54890/1‑300 (MQ=255)
                                        aGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAgg                                                                                                                                                                                                                                         >  2:61899/1‑301 (MQ=255)
                                          gatgagtatggTGGTTAACGGTGAATATCGTCCTCCAGACACATAAAAAGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGGGGTGATGATGCGATACAAAGTTTTATGGGCGATGACGCCAGTGCCAGCAGCTACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAgg                                                                                                                                                                                                                                         <  1:34098/289‑1 (MQ=255)
                                                           aCGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAAc                                                                                                                                                                                                                      <  2:17228/301‑1 (MQ=255)
                                                                              tgctcttccgatctacacCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTg                                                                                                                                                                                                   <  2:672/287‑1 (MQ=255)
                                                                                            acacCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGACTGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGACCCTGAACGATGTCACCACGGGCAACCCGACGCTAACCATCAACCAGGACACCTCCAACACGCAAACGGCGATCAAAGACTGGGTGagataggaatagcg                                                                                                                                                                                     >  1:672/1‑288 (MQ=255)
                                                                                                   ccTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATa                                                                                                                                                                              <  2:268828/301‑1 (MQ=255)
                                                                                                   ccTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCACAGCGCAAACGGCGATTAAAGCCTGGGTGAATGCCTACAACTCGCTAATa                                                                                                                                                                              >  1:70639/1‑301 (MQ=255)
                                                                                                               gacgacaccaccGGTCAGCGGTGATGATGGGCCACAAAGGTTGATGGGCGATGACGCCGGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGACAACAACGTGGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTcag                                                                                                                                                                   <  1:392133/289‑1 (MQ=255)
                                                                                                                            ttCAGCGGTGATGTTGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGATGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAAt                                                                                                                                                     <  2:277052/300‑1 (MQ=255)
                                                                                                                                       tgatgCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTa                                                                                                                                          <  2:410417/301‑1 (MQ=255)
                                                                                                                                               tACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCggg                                                                                                                                  <  2:70720/301‑1 (MQ=255)
                                                                                                                                                                   gACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACCACTCGATAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATCGCCAGAGTTCTa                                                                                                              >  1:312121/1‑301 (MQ=255)
                                                                                                                                                                    aCGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAg                                                                                                             <  2:416613/301‑1 (MQ=255)
                                                                                                                                                                       ccAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATCAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACAAAATACACCGCCGTAGATGCCGGAGCTGATAGCCAGATTTCGATCaa                                                                                                          >  1:13266/1‑301 (MQ=255)
                                                                                                                                                                             caagcagcAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTg                                                                                                     <  1:136512/298‑1 (MQ=255)
                                                                                                                                                                               agcagcAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCGTCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGCCCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTgaa                                                                                                   >  2:59800/1‑298 (MQ=255)
                                                                                                                                                                                 cagcaACGGTAGGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACt                                                                                                 <  2:245780/300‑1 (MQ=255)
                                                                                                                                                                                    caACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCt                                                                                              >  2:402595/1‑300 (MQ=255)
                                                                                                                                                                                       cGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCggg                                                                                          >  2:55817/1‑300 (MQ=255)
                                                                                                                                                                                       cGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAAAACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGc                                                                                          <  2:5811/301‑1 (MQ=255)
                                                                                                                                                                                         gTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCg                                                                                         <  2:49502/300‑1 (MQ=255)
                                                                                                                                                                                          tATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGAc                                                                                       <  2:219571/301‑1 (MQ=255)
                                                                                                                                                                                             ggAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGATACCAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCggataatc                                                                                     >  1:225273/1‑294 (MQ=255)
                                                                                                                                                                                               aGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCa                                                                                   <  2:312474/300‑1 (MQ=255)
                                                                                                                                                                                                    tcGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGAGAGCCAGAGTTCTAGCAAGGGTGctgcattgcagttgacttaacctt                                                                             >  2:122430/1‑278 (MQ=255)
                                                                                                                                                                                                              cccagaattgcaaggtagccgacgacaacGACACCATCGAGAACCGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCACCTGAACCTGAACGATGTCACCACGGTCAACCAGACGCTAACCATCACTCAGGACACCACCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTGCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCa                                                                   <  1:54837/277‑1 (MQ=255)
                                                                                                                                                                                                                                   tcacaaacGTCGCCAACGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACGCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCAGACGCAGTTGAAATCGATg                                                <  1:61831/294‑1 (MQ=255)
                                                                                                                                                                                                                                   tCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCAGACGCAGTTGAAATCGATGc                                               <  2:60959/300‑1 (MQ=255)
                                                                                                                                                                                                                                                        aCAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCAAGCTGCGGACGATTCAGACGCAGTTGAAATCGCTGCTTAGTAATACCGTCAGttctt                          >  1:287256/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                    ccATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCAGACGCAGTTGAAATCGATGCTGAGTAATACCGTCAGCTTTTCCAGGCATaaaa              >  1:398921/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                           cGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCGTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCAGACGCAGTTGAAATCGATGCTGAGTAATACCGTCAGTTCTTCCAGCTATAAAACGTTg         <  2:225495/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gcgcTGGAAAACATCCCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCAGACGCAGTTGACATCGATGCTGAGTAATACCGTCAGTTCTTCCAGCTATAAACCGTTGGCGCAGa  >  1:168123/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                             
CCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTTCTAGCAATGGTGCACTGCTCGGCGACTCCACGCTGCGGACGATTCAGACGCAGTTGAAATCGATGCTGAGTAATACCGTCAGTTCTTCCAGCTATAAAACGTTGGCGCAGA  >  NC_000913/2004330‑2004901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: