Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,296,191	A→C	34.1%	intergenic (+397/+245)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	4,296,191	0	A→C	34.1%	37.6 ‑0.3 0.0	54	intergenic (+397/+245)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  new base (10/9):  ref base (16/19):  total (26/28)
Fisher's exact test strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

GCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCC  >  NC_000913/4295898‑4296483                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         gCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc                                                                                                                                                                                                                                                                                                    <  1:940423/300‑1 (MQ=35)      aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATACGCCAGACAGCGCAATAGCATGATTTAGCGTGACTTTGTAGGTCGGATAAGGCGTTTATGCCGAATCCGACCTCAACGCCTGATGAGACGCTTAACGCGTCGTAGCa                                                                                                                                                                                                                                                                                              >  1:644483/1‑301 (MQ=255)                 cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTTGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCGGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTTTCAGGCATCCGcc                                                                                                                                                                                                                                                                                    >  2:864854/1‑300 (MQ=255)                    atatggtgttcagtacgcatcgggtgtgaaCGTCGGATGCGACGCGTACCG‑TGTCTTATCAGGCCTACG‑CTGCCAGCCGAAATAGCCTGATTTATCG‑TGATTTTGTCGGGCTGCTAAGGCGTTTACGCCGCATCCGCCATCAACGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAc                                                                                                                                                                                                                                                                                <  2:155342/273‑1 (MQ=11)                    aTAAGGGGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAc                                                                                                                                                                                                                                                                                <  1:941719/301‑1 (MQ=25)                    aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAc                                                                                                                                                                                                                                                                                <  1:101015/301‑1 (MQ=35)                    aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAc                                                                                                                                                                                                                                                                                <  1:325869/301‑1 (MQ=35)                              tagtgcgcctcggcacccAACGGCGGATGCGACG‑TTATCGCCGGCTTATCAGGCCTACGCCGGACAG‑CGCATTCGCTTGATTCATCG‑TGATTTTGTAGGTCGGATAAGGCGTTTCCGCCGCATCCGGCATAAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAGTAg                                                                                                                                                                                                                                                                      <  2:208223/284‑1 (MQ=11)                                      atcgtagaTCAAAGCCTGATGCACAGCGTAACC‑GGTCTTATCAGGCAGACGGCGGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGGTAAGGCGTTTACGCCGCAGACGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAttt                                                                                                                                                                                                                                                              <  2:950278/294‑1 (MQ=11)                                           aCATCAACGCCTGATGCGACGCTTACCG‑CGTCTTATCAGCCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                                                                                                                                                                                                                                          <  1:495713/300‑1 (MQ=17)                                                       gaggCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc                                                                                                                                                                                                                                                                                                       <  2:2092/240‑1 (MQ=25)                                                       gATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc                                                                                                                                                                                                                                                                                                       >  1:2092/1‑243 (MQ=35)                                                        ttGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCATCCTG‑CGCAATAGCCTCATTTAGCG‑TGATTTTTTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                                                                                                                                                                                            <  1:337337/300‑1 (MQ=255)                                                         tGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAAGAGCCTGATTTAGCGTGCTTTTGTCGGTCGGATAAGGCGGTTATGCCGCATTCGACATCACAGCCTGAGGCGACGCTTAACGCGTCGTATCAGGCCTACGCCCGCCAGCGCAAAACCATGATTAGCCGTGAGTTGGTAGGTCg                                                                                                                                                                                                                                           >  2:697570/1‑301 (MQ=11)                                                                cTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAgg                                                                                                                                                                                                                                    >  1:691646/1‑301 (MQ=34)                                                                        cgTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGCATTAGCGTGATTTTGTAGGTCTGATACGGCGTTTATGCCGCATCCGACCTCACCGCCTGCTGCGACGCTTACCGAGTCTTGTCAGGCCTCCGCCCGACAGCGCAATATCCTGCTTTGGCTTCATCTTGTACGTCGGagccgtcgtttaa                                                                                                                                                                                                                             >  2:308227/1‑289 (MQ=11)                                                                          tCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGc                                                                                                                                                                                                                           <  1:684709/301‑1 (MQ=17)                                                                            ttATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCg                                                                                                                                                                                                                         >  1:941560/1‑301 (MQ=18)                                                                                  ggCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                                                                                                   >  1:876939/1‑301 (MQ=25)                                                                                    ccTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCTGTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGAc                                                                                                                                                                                                                 <  1:287360/301‑1 (MQ=25)                                                                                       aCGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTATGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTCTTTCGCGTTATTTTCTCTATCGGATACGGCGTTTacccccccctctacct                                                                                                                                                                                                               >  2:85278/1‑285 (MQ=11)                                                                                       aCGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTTTACGCCGCCTCCCAcct                                                                                                                                                                                                               >  2:469536/1‑298 (MQ=18)                                                                                          ccAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaa                                                                                                                                                                                                            <  1:534311/300‑1 (MQ=25)                                                                                                    gcAATTGCCTG‑TTTGGCGTTGATTTTGTAGGTCGAATAAGGCGTTTACGCCGCATCCGACATCAACGCCAGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg                                                                                                                                                                                                   <  2:757521/300‑1 (MQ=255)                                                                                                     caagagctagATTTGGCG‑TGATTTTGTGGGGAGGCTAAGGCGTTTACGCCGCACACGGCATAAACGCCGGATGCGAGGCTTGCCTGGTCTTATCAGGCCCACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                                                                                  <  2:493028/291‑1 (MQ=11)                                                                                                          gCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCTCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGACTACGCCAGACAACGCAATAGCCTGCTTTCGCGTGATTTTGTAGGTCGGCTAAGGCGTTTACGCCGCAACGGACATCAATGCCTGCTGGCACGc                                                                                                                                                                                             >  2:636567/1‑300 (MQ=11)                                                                                                          gCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                                                                                                                             <  1:80605/300‑1 (MQ=21)                                                                                                                             ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATCGCCTGATTTAGCGTTAATTTGTAGGTGGGATAAGGCGTGTACGCCGCATCCGACTTCAATGCGTgtctattatacacatctgacgctgccga                                                                                                                                                                           >  2:713025/1‑273 (MQ=11)                                                                                                                               ggaGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                         <  1:589937/298‑1 (MQ=12)                                                                                                                                       gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACTCCGGATGCGACGATTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCTTATCCGGGCTatcttaa                                                                                                                                                                 >  1:725110/1‑294 (MQ=11)                                                                                                                                       gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAAGGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTatcttaaa                                                                                                                                                                >  2:888225/1‑294 (MQ=255)                                                                                                                                       gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatcttaa                                                                                                                                                                 >  1:223765/1‑294 (MQ=2)                                                                                                                                       gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTGATCCGGCCGAcattaaa                                                                                                                                                                >  1:391612/1‑295 (MQ=255)                                                                                                                                        tcaagcctgtcaccccgcACCCAGCATCAACCGCGTATGTGCCGTTTGCCGGGTCTTATCAGGTCTAGGCCAGACAGCGCGAGTGCCTGCTTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGTAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatcttaacc                                                                                                                                                               <  2:391608/287‑9 (MQ=11)                                                                                                                                               gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                                                                    <  1:841435/289‑4 (MQ=9)                                                                                                                                               gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCCGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCCCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCAGACTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGCCCTatct                                                                                                                                                                    >  2:841402/1‑286 (MQ=11)                                                                                                                                                                      tgCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCATACGCCAGCAAGCGCAATAGCCTGATTTAGCGTGATTTCGTATGTCCGATCAGGCGTTTAGCCCGCACTCGGCGTTACATCCTGCAGCTACGCTGAACGCGCCTCATCAAGCCGATCCGAGACAGCACGATAGCCTGAgttctggcacttttt                                                                                                                                 >  2:495705/2‑289 (MQ=11)                                                                                                                                                                                                                                                         acaaGCCGTTTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCTTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                            <  1:713164/299‑1 (MQ=11)                                                                                                                                                                                                                                                         aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                            <  1:864887/301‑1 (MQ=2)                                                                                                                                                                                                                                                          aaaGGCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGACGGTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                           <  1:747643/300‑1 (MQ=2)                                                                                                                                                                                                                                                          aaaGGCGTTTACGCCGCATCACAACTCACCGCCGGATGCGCTCCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                           <  2:941515/300‑1 (MQ=11)                                                                                                                                                                                                                                                          aaaGGAGTTTACGCCGCATCCGACATAAACGCCTGATGCGACGCTGCCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                           <  1:469543/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      gCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCTTGATTTTGTAGGTCGGATACGGCGTTTACGC‑C‑GCATCCGACCTCAATGCCTGATTCTACGCTTGCCGCGTCATCTCAGGCCTATCTTACCCGTTGGTTCAtttt                               >  2:691780/1‑301 (MQ=11)                                                                                                                                                                                                                                                                         gCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCa                            >  1:399192/1‑301 (MQ=255)                                                                                                                                                                                                                                                                          cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGAATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                           >  1:225122/1‑301 (MQ=255)                                                                                                                                                                                                                                                                            tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                          >  1:700648/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATATTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTGTGTAGGTAGGATAAGGCGTTTA‑‑CGCAGCATCCGACATCAATGCCTGATGCGACGCGTGCCGCG‑‑‑TATCAGGCCTATCTTAACCGTTGGTTAATGTTTCAAAct                        >  1:840902/1‑299 (MQ=11)                                                                                                                                                                                                                                                                                  aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGCATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCCACGCTTGCCGCGGCTTATCAGCCCTATCTTAACCGTTGTGTAATTTTTCCACCTCTTc                    >  2:475242/1‑300 (MQ=11)                                                                                                                                                                                                                                                                                   tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                   >  1:123425/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      aCGCCTGATGCGACGTTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc               <  1:903106/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCTATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GAATGCGACATCAATGCCTGATGCACCGGTTGCCGCGTCTTATCAGGCATATTTTAAccctctcacttatctacatctgaagctgccta              >  2:52161/1‑271 (MQ=11)                                                                                                                                                                                                                                                                                                  ccGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc   <  1:636591/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ccGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc   <  1:888265/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   cGCTTGCCGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc  <  1:292687/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         GCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑CGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCG‑TGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCC  >  NC_000913/4295898‑4296483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.