Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_000913 | 2,660,628 | 2 | .→A | 1.3% | 0.1 0.0 ‑0.0 | 158 | coding (904/1215 nt) | iscS | cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent |
Rejected: E-value exceeds prediction threshold. | ||||||||||
Rejected: Polymorphism does not pass frequency cutoff. | ||||||||||
Rejected: Polymorphism prediction not supported by minimum number of reads on both strands. | ||||||||||
Reads supporting (aligned to +/- strand): new base (1/1): ref base (80/76): total (81/77) | ||||||||||
Fisher's exact test strand distribution p-value = 1.00e+00 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.24e-01 |
Only 100 of 162 total aligned reads displayed.
CATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGA > NC_000913/2660330‑2660927 | aTTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTTAAGCTGACGTT‑‑gag > 2:88996/1‑301 (MQ=255) cTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTc < 1:189532/300‑1 (MQ=255) cTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCg > 1:333030/1‑301 (MQ=255) gTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCgg > 1:881692/1‑300 (MQ=255) gTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCgg > 1:396025/1‑300 (MQ=255) tCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCgt > 1:825151/1‑301 (MQ=255) tCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACATGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCGGCGACTCACCTTCAACGTAGTTGAAGCTGAAGTT‑‑GAGAATGTTCGGCGCACCgt > 1:801566/1‑301 (MQ=255) ccccTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGACATTGATAGAGCTATGTGCCAGCTCGTCGTTCAGCACAACCCTACTCCGCACGTATTATTTTTT‑TATTTTTCCTTCCGTTCATGCGGAACCTTTAGAAACTTCTAGGTCTTTCAGCGTTCTATTCCTCTCCTCCTCTTCCCCGTCTTTTTAGCTGACTTT‑‑TAGAATGTTCGGCGCACCGTGTTcc < 1:377856/300‑1 (MQ=255) cTGCTTGTACATTTCCCCCAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTc < 2:632271/283‑1 (MQ=255) gCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGTTGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTTGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTGGTCGTTCAGCCCCAGTGCGCGCAGCCCGTAGGACGGTTC‑CAGGCTTGCTGACGTACTGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCCCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGGTGACGTT‑‑GAGCATGTTCGGCGCACCTTGTTCCTGGt > 1:148683/1‑300 (MQ=255) gCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGtg > 1:305785/1‑300 (MQ=255) gCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGACCCAGCGCGCGCAGCACGCAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCCGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAGAATGTTCGGCGCACCGTGTTCCAgg > 2:385614/1‑301 (MQ=255) ttGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATAGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCAc < 2:333024/301‑1 (MQ=255) acaaaTTCCCCAAAGCGGAGAAAGGTCACGCATACGACCGAAGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCg < 2:525005/297‑1 (MQ=255) aCATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGGAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGAGAGGTCTTTCCGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGGTGACGTT‑‑GAGAATGTTGGGCGACACGTGTTCCAGGTCACCGtt > 2:3664/1‑301 (MQ=255) cATTTCCCACAGCGTAGACAGGTCACGCAGACGCCCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGCTCTCTTCTTCAGTAGTAAAACGCCCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGATC‑GAGGCTTGCTGACGAACAGGCGGAACCGGACGAAACTGCGAGGGCTTTCAGCGCCATAGTCAGAGAATAGCATTAAAAGTCGTTGGATCTTACGTT‑‑GAGAATGTTCGGCTCACCTTGTTccggctcccagtt > 2:597733/1‑289 (MQ=255) cATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGtt > 1:352954/1‑300 (MQ=255) aTTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCg > 1:525014/1‑297 (MQ=255) ttCCCGCAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAgg < 2:829126/301‑1 (MQ=255) tCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGt > 1:563862/1‑301 (MQ=255) gCGGGGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTTCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATTAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTaacatt > 1:4930/1‑296 (MQ=255) gagaATGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtcttt > 2:260470/1‑300 (MQ=255) gagaAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTCGTTGAAGGTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGTTCACCGTTCAGGTAAActtctt > 2:875802/1‑300 (MQ=255) ggcACCCAGACGTCCCATGGATGTACGACATAACTCAG‑TGTTGTAGTCGATCTCTTATTCCGTAGTAAAACGACTCAAAGAGAAACGGATAGAGCCATTTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGatat < 2:187952/297‑1 (MQ=255) acgacCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGc < 2:110447/299‑1 (MQ=255) attgATTTACGTACTAACATGAGTGGT‑TAGTCGATCTCTGCGGTAGTAGTAAAACGACCGAAAGAGACACCGATAGAGCTACGTGCCAGCTCGTCGTGCAGCACCAGCGCGCTCAGCACGTAGGACGGTTC‑GAGGCTTTCTGACGTACAGGCGGAACCTGAATAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGATGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCcac < 2:568736/297‑1 (MQ=255) gAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGGAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACg > 1:335103/1‑301 (MQ=255) aactaactCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACg > 2:148248/1‑300 (MQ=255) ctaactCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGACGAAACTGCGAGGTCTTTCAGCGCCCTAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCCCCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACCGACGGGTACGcc > 2:426053/1‑299 (MQ=255) actCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGc > 1:710490/1‑300 (MQ=255) a‑TGGTGTAGTCGATCTCTTCTTCAGTTGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGTTATCTTTGCTGCCGTTCCACAGACGGTTACGCAGGCcgcga > 2:163003/1‑299 (MQ=255) a‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCgcgc > 2:40909/1‑300 (MQ=255) ggTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGACACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGa > 1:616706/1‑301 (MQ=255) ggTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTACTTGCAGCTGACGTT‑‑GAACATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAGTTCTTCGATATCTTTGCTGCCGTTCCACCGACGGTTACCCAGGccccctaga > 2:779326/1‑294 (MQ=255) gtgtAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTATAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCCCCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTTATGCCGTTCCACAGACGGTTACGCAGGCAGCGCAgc > 2:301228/1‑299 (MQ=255) gtgtAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGa > 1:838332/1‑300 (MQ=255) gTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGTATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGAACGGTTC‑GAGACTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGAGCCATAATCAGCGACTCACCTTACACGAAGTTGAAGCTGCCGTT‑‑GAGAATGTTCGGCGCACCGTGTTCACAGTCACCGTTCAGGTAAAGTTCTTCTATCTCTTTGATGCAGTTCCACAGACGGTTCCTCAGGCCGAGCCGAcctgc > 2:582595/1‑296 (MQ=255) aTCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCcac < 1:460636/267‑1 (MQ=255) ctctTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGACGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTCGGCAGGCCGCGCAGACGTTCCATCTCg > 2:700133/1‑301 (MQ=255) gtagtaAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtc < 2:251498/288‑1 (MQ=255) gtagtaAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtc > 1:251502/1‑288 (MQ=255) gACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCCTCCCTTCTTTtg > 2:55313/1‑300 (MQ=255) aCCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGAGATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTtgcg > 2:243586/1‑301 (MQ=255) gaAACGGGTAGAGCGATGTGCCCGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTGTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGc < 1:869148/227‑1 (MQ=255) aaaCGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAgg < 1:597369/301‑1 (MQ=255) aaCGGATGGAGTTAGGTGGCAGCTGGTCGTCCAGGCCTAGCGCCCACAGCTCGTA‑GACCGTTCTGAGGCTTCCTGACGTGAAGGCGAAACCGGCAGCAACTGCGAGGTCTTTAAGCGCCATATTCAGCGACTCACCTTCAACGTAGGTGAAGCAGACGTT‑‑AAGAATTTTCGGCGCACCGCGTTCCAGGTCACCGTTCCGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTGCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGc < 2:557455/301‑1 (MQ=255) ggATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtc < 1:308093/263‑1 (MQ=255) ggATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCt > 1:431062/1‑300 (MQ=255) ggATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGACGACGTTCCAtc > 2:308087/1‑263 (MQ=255) gagCTATGTGCCAGCTCGTCGTTCAGCCCCGGCGCGCGCAGCACGTAGGCCGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGccc < 1:3664/300‑1 (MQ=255) gagCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGccc > 1:412712/1‑300 (MQ=255) agCTATGTGCCAGCTCGTCGTTCAGCCCCAGTGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGccc < 1:104915/299‑1 (MQ=255) gCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGc < 1:134215/235‑1 (MQ=255) gCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTAAGc > 2:134214/1‑235 (MQ=255) gCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTACGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCGACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCAtt > 1:409487/1‑301 (MQ=255) ttATGTGCCAGCTCGGCGGTCAGCCCCAGCGCGCGCAGCACTGAGGACGGTTC‑GAGGCTTGCGGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTG‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTc < 2:881655/300‑1 (MQ=255) cTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTc < 1:325827/301‑1 (MQ=255) aTGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAACCTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCACCTCGGTCGCCATCCTTTCTTTTGCGCTGCGACAGCCATCGCCCATTcc > 2:357304/1‑300 (MQ=255) gtgCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTCCGCAGGCCGCGCAGACGTTCCATCTCGGTCACCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCcga > 1:767671/1‑300 (MQ=255) tgCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCcgac < 1:108137/300‑1 (MQ=255) cTCGTCGGTCAGCCCCAGCGCGCGCAGCACGTAGGACGTTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTgg < 1:522397/301‑1 (MQ=255) tcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGa < 1:300652/300‑1 (MQ=255) gtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGAGAGGCCTCGCCCATTCCGACGATCGGGTGAACa > 2:259950/1‑301 (MQ=255) gtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAAACGTTCCATCCCGGTCGACATCCCTTCTTTTGCAAGTCGATAGGCCTCGCCCATTCCGACGCTCTGGTGAACa > 2:854276/1‑301 (MQ=255) gCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCag > 1:130852/1‑301 (MQ=255) cccAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCagag < 1:900914/301‑1 (MQ=255) cgcAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGaa < 1:262995/300‑1 (MQ=255) cgcAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGaa < 1:163005/300‑1 (MQ=255) caCGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGc < 1:558155/298‑1 (MQ=255) aCGTAGGACGGTTG‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATAc < 1:493615/301‑1 (MQ=255) ggTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCCCGt > 1:708155/1‑301 (MQ=255) gTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTg > 1:629132/1‑301 (MQ=255) gTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTg > 1:613420/1‑301 (MQ=255) gCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACAATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAcccccgccgg > 2:233032/1‑300 (MQ=255) tACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTg < 1:242769/301‑1 (MQ=255) aaCAGTCGGAACCTAAATAAACTGCGAGGTCTTCCAGCGCCATAATCGGCGACTCACCTGCAACTTAGTTGAGGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTg < 1:754623/300‑1 (MQ=255) gCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTgcg < 1:88997/298‑1 (MQ=255) gTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACcg > 1:523119/1‑247 (MQ=255) tttttCCGCGCCATAACCAGCGCCGACCCTTCAATTGAGTTGAAGCGTACGTT‑‑GAGAACGTTCGGCGCACCGTGTTCCAGCTCACCGTTCAGGTAAACTTCTTCGATCTCTGTGACGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGTTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTacgac < 2:617186/299‑1 (MQ=255) ttttCCGGGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGATGACGTT‑‑TAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTacgacg < 2:710469/300‑1 (MQ=255) ctccATAATCCGCGACTCACCTTCAACGTAGTTGAAGGTGACGTT‑‑GGGAATGATCGGAGGACCGTGTTCCAGTTCACCGTTCAGGTAAACTTCGTCGATACCTTTGATGCCGTGCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac < 2:473585/299‑1 (MQ=255) ctcactAATCAGCGACTGACCGTCAACCTAGGTGCAGCTGACGTG‑‑GAGAAGGTACGTAGCAGCGTGTTACACGTCTCTGGTCACGTAAACGTCTTCGATATCTTGGATGCCGTTCCTCTATCGGTTACGCAGGCCTCGCAGACGTTCCATCTCGGGCGCCATCTCGTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac < 2:431055/296‑1 (MQ=255) ctcaaacagcatcGACTCACCTTTAACGTAGTGTAACGTGAAGTT‑‑GGGCATGTTCGGCGCACCGTGGTCCAGGGACCCGGTCAGGTAAGATGCTTGGATAGCGTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGTAGACTTGTCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATGCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac < 2:339025/289‑1 (MQ=255) cgcCATAATCAGCGACTCACCTTCAACGGAGTTGAAGCTGACGTT‑‑GAGAATGGTCGTAGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGGTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac < 1:597750/301‑1 (MQ=255) cgcCATAAACAACGACTAACCTTCAACGTAGTTGAAGCTGACGTT‑‑CAGAATGTTCGGCGCACCGTGTTCAAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTACACAGACGGTTACGCAGGCCGCGCAGACGTTCCATATCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac < 1:243590/301‑1 (MQ=255) ccccataattatgggcccACCTTCACCGTAGTTGAAGCTGACGTG‑‑GACAAGTTTCGGCGCACCGTGTTCCAGGACACCCTTCAGGTAAACTTCTTCGTTATCTTTGATGCCGTTCCACCGACGGTTACGGAGGACGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac < 2:4930/284‑1 (MQ=255) gcCCTAAGCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GTGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac < 2:201725/300‑1 (MQ=255) ccataattcggtaCTCACCTCCAACGTAGTTGAAGCTGACGTT‑‑GGAAATGTTCGGCGCACCGTGTCCCAGTTCACCGGTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacataca < 2:825123/288‑1 (MQ=255) ccATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAg < 1:426060/301‑1 (MQ=255) gCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGaa > 1:833487/1‑300 (MQ=255) aatacccTTCAACGTATTTGAAGGTGACGTT‑‑GAGAATGTGTGTCTCACCGTGTTCCAGGTCACCGTACAGGGAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTGACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTGGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGAGGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATAcc < 2:709946/296‑1 (MQ=255) aCTCACCTTCAACGAAGTGAAAGATGACGGT‑‑AGAAATGTTCGGCGGACCTTGTTCCAGTGCACCGGTCAGGTAAACTTCTTCGATATCTTTGAGGCCGTTCCACCGGCGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATAc < 2:335097/300‑1 (MQ=255) aCTCACATTCAAAGGATGTGAAAGTGACGTG‑‑GAGAATGTTCGCGGCCACGTGTGCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGGTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATAcc < 2:352948/301‑1 (MQ=255) cACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCttt < 1:686127/301‑1 (MQ=255) cACCTTAAACGTAGTTGGAGCTGCCGTT‑‑GAGACTGTTCGGTGCACCGTGTTCCGGGGCAACGATCAGTTGAACTTGTTATAGTGCTTTGAGGCCGTTCCAAAGACGGTTACGCAGGCCGCGGAGACGTTCCATCTCGGTCGCCATTTCTGCTTTTGCGATGCGATAGGCCTCGCCCATCCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCttt < 2:767648/301‑1 (MQ=255) ttCAACGTAGTTGAAGCTGACGTT‑‑GGGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCggg < 1:55313/301‑1 (MQ=255) tgtaaCGGAGGTGAAGCTGACGTT‑‑GGGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCggg < 2:83299/298‑1 (MQ=255) cTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTtgtg < 1:878417/299‑1 (MQ=255) cGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCATCGCCCATTCCGACGATCTGGTGAACATGCAGAGTGCCGGAACGGATACAGCGCTCGTGACAGCCTGCGTGCATTTGCGATTCAACCCGTACGCGCGGGTTACGACGTACCCACCACGACACGATACCTTTCTAGCCaatcgtttcgtcaacgtg > 2:280581/1‑284 (MQ=255) tt‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGgaga < 1:779350/301‑1 (MQ=255) | CATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGA‑TGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTC‑GAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTT‑‑GAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGA > NC_000913/2660330‑2660927 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |