mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.26.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | 6-Gly_S4_L001_R1_001 | 915,184 | 260,058,404 | 100.0% | 284.2 bases | 301 bases | 99.4% |
| errors | 6-Gly_S4_L001_R2_001 | 915,145 | 261,513,507 | 100.0% | 285.8 bases | 301 bases | 91.3% |
| total | 1,830,329 | 521,571,911 | 100.0% | 285.0 bases | 301 bases | 95.4% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 113.3 | 3.6 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 1770 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 126 |
| Total length of all junction candidates (factor times the reference genome length) | ≥ 0.1 | 0.016 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.88288 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum coverage each strand | 2 |
| Polymorphism bias cutoff | 0.001 |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥2 bases |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 05:15:17 07 Jul 2015 | 05:16:00 07 Jul 2015 | 43 seconds |
| Read alignment to reference genome | 05:16:00 07 Jul 2015 | 05:19:53 07 Jul 2015 | 3 minutes 53 seconds |
| Preprocessing alignments for candidate junction identification | 05:19:53 07 Jul 2015 | 05:20:48 07 Jul 2015 | 55 seconds |
| Preliminary analysis of coverage distribution | 05:20:48 07 Jul 2015 | 05:23:35 07 Jul 2015 | 2 minutes 47 seconds |
| Identifying junction candidates | 05:23:35 07 Jul 2015 | 05:23:35 07 Jul 2015 | 0 seconds |
| Re-alignment to junction candidates | 05:23:35 07 Jul 2015 | 05:24:00 07 Jul 2015 | 25 seconds |
| Resolving alignments with junction candidates | 05:24:00 07 Jul 2015 | 05:25:26 07 Jul 2015 | 1 minute 26 seconds |
| Creating BAM files | 05:25:26 07 Jul 2015 | 05:27:06 07 Jul 2015 | 1 minute 40 seconds |
| Tabulating error counts | 05:27:06 07 Jul 2015 | 05:29:49 07 Jul 2015 | 2 minutes 43 seconds |
| Re-calibrating base error rates | 05:29:49 07 Jul 2015 | 05:29:50 07 Jul 2015 | 1 second |
| Examining read alignment evidence | 05:29:50 07 Jul 2015 | 07:21:26 07 Jul 2015 | 1 hour 51 minutes 36 seconds |
| Polymorphism statistics | 07:21:26 07 Jul 2015 | 07:21:28 07 Jul 2015 | 2 seconds |
| Output | 07:21:28 07 Jul 2015 | 07:22:38 07 Jul 2015 | 1 minute 10 seconds |
| Total | 2 hours 7 minutes 21 seconds | ||
