Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,407	C→A	M272I (ATG→ATT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,407	0	C	A	100.0%	147.0 / NA	43	M272I (ATG→ATT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/21);  total (22/21)

CCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTC  >  NC_000913/4116110‑4116701                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       cccACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATa                                                                                                                                                                                                                                                                                                     <  2:218838/301‑1 (MQ=255)    aCGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAag                                                                                                                                                                                                                                                                                                  <  2:48873/301‑1 (MQ=255)              ccGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTTGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGACCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCCGAGTAAagccagcagtt                                                                                                                                                                                                                                                                                         >  1:246767/1‑292 (MQ=255)                      cGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCTGAACCCCATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACTCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGgtg                                                                                                                                                                                                                                                                                <  2:113662/301‑1 (MQ=255)                              tctcagaCGGGACCCAGTACGGCGCCCCCACCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAACCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCATCCACGGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTGTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGc                                                                                                                                                                                                                                                                        <  1:252015/295‑1 (MQ=255)                                 cATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGcaga                                                                                                                                                                                                                                                                     >  2:252313/1‑298 (MQ=255)                                           ccAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCttt                                                                                                                                                                                                                                                            >  1:303016/1‑300 (MQ=255)                                                     ttcagacgtgtgctcttccgatctaCCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAAc                                                                                                                                                                                                                                                  <  2:33140/276‑1 (MQ=255)                                                      cACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCAGAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGCCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGACTTAACCGCTTCCTGGAAAGAGTTCATCAGAATAAAGCAGCCAGTTCTACAGGTGTTGTTCTCAAGACGTTCTTTCacacacccatt                                                                                                                                                                                                                                                >  1:170442/1‑296 (MQ=255)                                                             cccGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCaa                                                                                                                                                                                                                                           <  2:38853/299‑1 (MQ=255)                                                                     aTGCCGTAAACACATAAACACCATTGGTGTTTTGCGCTTTGGTGGCGAAATACTCGGAATCGTAGGCGTCGTTACTCAACTTCATGTCATAGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCTCCTTACAACGCAGAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGCTTTAACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTGCGCCATCCCTTCGTACACGCACAACTGACCAAACAGCGCGGc                                                                                                                                                                                                                                 <  1:238953/301‑1 (MQ=255)                                                                         cGGAACCACATACACACCAGTGGTGTTTTGCACATTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCctgc                                                                                                                                                                                                                             <  2:48923/301‑1 (MQ=255)                                                                             aCCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACCGatcggaagacggtcgtgtagggg                                                                                                                                                                                                                         >  1:33107/1‑279 (MQ=255)                                                                                               gtgtgtTGCACGTTGGTGGCGAACTATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGgag                                                                                                                                                                                                        <  2:48986/300‑1 (MQ=255)                                                                                                   ctatctcTTGGGGGGAGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCAGTTCATCGCTCAGCCACTGAATGGACGCGCCGGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTgg                                                                                                                                                                                                   <  1:65010/295‑1 (MQ=255)                                                                                                         cTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGagatcg                                                                                                                                                                                             >  2:65086/1‑296 (MQ=255)                                                                                                          tttGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgc                                                                                                                                                                                            <  2:2860/301‑1 (MQ=255)                                                                                                           ttGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgcg                                                                                                                                                                                           <  2:366590/301‑1 (MQ=255)                                                                                                                   gAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgccgcc                                                                                                                                                                                    <  2:323009/300‑1 (MQ=255)                                                                                                                              aaTCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAAt                                                                                                                                                                        <  2:247057/301‑1 (MQ=255)                                                                                                                                   tAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAg                                                                                                                                                                   <  2:303388/301‑1 (MQ=255)                                                                                                                                                   aCTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTTACCAAACAGCGCGGCCTGCTGTCCACCGGCGTCTCCGGAGATTGGCATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAACggttgatt                                                                                                                                                    >  1:116782/1‑293 (MQ=255)                                                                                                                                                     ttcatttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCGGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCgg                                                                                                                                                 >  2:303080/1‑301 (MQ=255)                                                                                                                                                         ttcacacgcgCAGCAACGGAAGGGATGGCCCGGCCATAAACACCGCACCTTCCAACGCATATTTCACTCCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCAGTTTTCTGATTTCACCGCTTTCTCGCAAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaaga                                                                                                                                             <  1:377336/295‑1 (MQ=255)                                                                                                                                                                                              aaaCACCGCACCTGACGACGCATAGTTCACTTCGCCAGGCGGGCCTGAGGCGAGGGTGGTCAGCAGGCCGCTTTCTGATGTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATcc                                                                                                        <  1:27858/301‑1 (MQ=255)                                                                                                                                                                                                       cccTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCGGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTcc                                                                                               <  1:302708/300‑1 (MQ=255)                                                                                                                                                                                                          ttCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAg                                                                                             >  2:243766/1‑300 (MQ=255)                                                                                                                                                                                                           tCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCa                                                                                           >  2:368102/1‑301 (MQ=255)                                                                                                                                                                                                           cccAACGCATAGTGCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCGGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCa                                                                                           <  2:168231/300‑1 (MQ=255)                                                                                                                                                                                                             cAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt                                                                                         >  2:21113/1‑301 (MQ=255)                                                                                                                                                                                                                                     cGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCCTCCCGCCCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATgg                                                                 >  1:322052/1‑301 (MQ=255)                                                                                                                                                                                                                                                cGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaacaa                                                      >  2:182969/1‑301 (MQ=255)                                                                                                                                                                                                                                                cGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaacaa                                                      >  2:118007/1‑301 (MQ=255)                                                                                                                                                                                                                                                      tggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTAGGGATGTTGAACAACATGGt                                                >  2:20330/1‑301 (MQ=255)                                                                                                                                                                                                                                                                gCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGATAGGCg                                      >  1:98714/1‑301 (MQ=255)                                                                                                                                                                                                                                                                 ccGTTTTCTGATTTCACCGCCTTCTCGCCAGTGTTCATAAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCg                                      <  1:182762/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        cTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTa                              >  2:316870/1‑301 (MQ=255)                                                                                                                                                                                                                                                                        cTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAAGGGCGTTGGTGTa                              >  2:299590/1‑301 (MQ=255)                                                                                                                                                                                                                                                                               cACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCTCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCATTACAGGGTATGGATGTTGAACAACATGGTACGAAAGGCGTTGGTGTAATCGGt                        >  2:40104/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    cTTGCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACAt                   <  2:255800/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ctcGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGCTGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca              >  1:163540/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                          cGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTACTCGGTCACATGGACACg            >  1:44657/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                    catcaGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCAAGCATTTTGTCGTACCGGTCCCAGGTATGGATGTTGAACAACATGGTACGAGCAGCGTTGGTGTAATGGGTCACAAGGTCACGGCACTGAGtg  >  1:69968/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       CCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTC  >  NC_000913/4116110‑4116701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑