Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,846,674 | 0 | T | G | 46.4% | 70.3 / 20.9 | 56 | T247P (ACC→CCC) | hycD | hydrogenase 3, membrane subunit |
Reads supporting (aligned to +/- strand): ref base T (8/22); new base G (26/0); total (34/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.66e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCC > NC_000913/2846524‑2846822 | cAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGt < 1:356121/151‑1 (MQ=255) gCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGgtttc > 1:418724/1‑149 (MQ=255) ccAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCa < 1:708544/151‑1 (MQ=255) aaaCCCACCCCAGGTAATGCGCGGAGTAATATCAAGACTCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACTCCGACCACCAGTTTTACGATTTCAATCACCAGCCCCGGCAGCAGTCCACCGGCGGTGCAAGGttctgtt > 1:230792/1‑146 (MQ=255) aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTg < 2:877665/151‑1 (MQ=255) aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCCttt > 1:35919/1‑150 (MQ=255) ccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTGTCCCCAc > 2:976074/1‑151 (MQ=255) ccagGTAATGCGCGGAGTAATATAAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑GAGGGTTCCATTTGTCCCCAc > 1:812362/1‑151 (MQ=255) agGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACgg < 2:162794/151‑1 (MQ=255) agGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTTTTCCCCCgg > 2:222125/1‑151 (MQ=255) tAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTACATTTGTCCCCACGGAAt > 2:265051/1‑151 (MQ=255) aaTGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTTCATTTGTTCCCACGGaa > 2:404804/1‑149 (MQ=255) aaTGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTGTTCCCACGGaa > 2:426196/1‑149 (MQ=255) gcgcGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATaaa < 2:626309/150‑1 (MQ=255) gcgGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTTCCCACCGaaaaaaaa > 1:426805/1‑148 (MQ=255) ggAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑GAGGGTTCCCTTTGTCCCCCCGGAATAAACAccc > 1:576535/1‑151 (MQ=255) gTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCcga < 2:587775/151‑1 (MQ=255) tatCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTTTCCCCACGGAATAAACAcccc > 2:852323/1‑145 (MQ=255) gACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCCTTTTTCCCCACCGAAAAAAAAACCCCACGAACATCt > 1:648818/1‑150 (MQ=255) cGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGCG‑GAGGGTTTCATTTTTTCCCACCGACTAAACACCCCGACGAACCACTGc > 1:233094/1‑150 (MQ=255) aGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAAGTTTCCATTTTTCCCCACGGAATAAACACCCCGACGAACATCTGCAac > 2:93674/1‑150 (MQ=255) gACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑GAGGGTTCCATTTGTCCCCACGGAATAAAAACCCCGACGAACAACTGCAaca > 1:914447/1‑150 (MQ=255) gACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCCTTTGTCCCCACGGAATAAACACCCCCACGAACATCTGCCacac > 1:221364/1‑151 (MQ=255) ccATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAg < 1:893899/151‑1 (MQ=255) aTGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCCTTTGTCCCCACGGAATAAACACCCCCACGAACATCTGCAACaacacc > 1:28273/1‑147 (MQ=255) tGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGGG‑AAGGGTTTCCTTTGTCCCCCCGGGATAAAAACCCCGTCGGACAACTGCAACACCACCCGccg > 1:334080/1‑146 (MQ=255) gTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGttt < 2:444883/150‑1 (MQ=255) gTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGtt < 1:441802/149‑1 (MQ=255) gTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTc < 2:854760/151‑1 (MQ=255) tttCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCACGGAAAAAACACCCCGACCAACATCTGCAACACCACCAGCTGttt > 2:431287/1‑148 (MQ=255) ttCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAg < 2:531162/150‑1 (MQ=255) tCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAAGGTTCCCTTTGTCCCCCCCGAATAAAAACCCCCACGAACATCTGCAACACCACCCGCTGTTTCAgg > 1:605020/1‑150 (MQ=255) gAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTTTCCCCCCGGAATAAAAACCCCGACCAACATCTGCAACACCACCAGCTGTTTTAgg > 1:903612/1‑148 (MQ=255) cgcgATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑GAGGTTTTCATTTGTTCCCCCGGGATAAAAACCCCCACGGACATCTGCCACACCACCAGCCGTTTCAGGCTGGTAacc > 1:154348/1‑151 (MQ=255) cgATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGtttagatcggaagagcgt < 2:190255/151‑16 (MQ=255) gATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCAt < 2:700126/151‑1 (MQ=255) aaCCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTc < 1:732193/151‑1 (MQ=255) ggACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGAc < 1:452366/151‑1 (MQ=255) gACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGAc < 2:837237/150‑1 (MQ=255) gACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAAGGTTTCAATTGTCCCCCCGGGATAAACACCCCCCCGAAAATCTGTAAAACCACCCGCCGGTTTAGGCAGAATCCCCCATTCAAGAc > 1:342654/1‑150 (MQ=255) cGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTT‑TAAGTTTTCATTTTTCCCCCCCGAATAAACACCCCCACGAACAACCGCCAACCCACCCGCCGGTTTCGGCCGATAaccccctttattatgcc > 1:375090/1‑135 (MQ=255) gACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCACGGAAAAAAAACCCCGCCGAACATCTGCCAAACCACCAGCCGGTTCAGGCTGATACCCCCATTCATGACGCCaaa > 1:800354/1‑150 (MQ=255) ccaccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTTTCCCCACGGGATAAAAACCCCCACGAACATCTGCAACACCACCAGCCGTTTCAGGCTGATACCCCATTTCATGACGCCAAAgcc > 2:506684/1‑151 (MQ=255) caccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAgccg < 1:397385/151‑1 (MQ=255) accaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCACGGAATAAACAACCCCCCGAAAATCTGCAACACCACCCGCTGTTTCAAGCTGCTAACCCCTTTCAAGAAGCCaaaagc > 2:182397/1‑146 (MQ=255) tttACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCCCCGAAAAAACACCCCCACGAACATCTGCAACACCCCCACCTGTTTTAGGCTGATACCCCCTTTCATGAAGCCCAAacccccgcc > 1:959548/1‑149 (MQ=255) ttACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTGTCCCCACGGAATAAAAACCCCGACGAACATCTGCAACACCACCAGCCGTTTCAGGCTGATACCCCCTTTTATGACGCCAAAgccgccgccgc > 1:846557/1‑150 (MQ=255) cGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTa < 2:739042/151‑1 (MQ=255) aTGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTAtt < 1:122326/151‑1 (MQ=255) tCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCCTTTTTCCCCCCGGAAAAAACACCCCCACGAAAAACCGCCAAACCACCAGCTGTTTCAGGGTGATACCCCATTTTATGAAGCCAAAACCGCCGCCGCCGTATTc > 1:610569/1‑145 (MQ=255) ccAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCgg < 1:869996/151‑1 (MQ=255) ccAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCg < 1:510454/150‑1 (MQ=255) gcagTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCCGCCGCTGTATTCAGAGAGCGGGCCCTcc > 2:373047/1‑146 (MQ=255) agTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCCCGGAAAAAACACCCCGACGAACATCTGCAACACCACCAGCTGGTTCAGGCTGATACCCCATTTCATGACGCCCAAACCGCTGCCGCTGTATTCAGAGAGCGGGCCCTcc > 1:990012/1‑144 (MQ=255) ccACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCt < 1:226119/151‑1 (MQ=255) cACCGGCGGTG‑AAGGGTTCCATTTTTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCCTTTCATGACGCCAAAACCGCTGCCGCTGTATTCAGAGAGAGGGCCTTCCTGCAGcccc > 1:645207/1‑149 (MQ=255) cggcggTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACCCCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTc < 2:493403/151‑1 (MQ=255) cggcggTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTc > 2:651460/1‑151 (MQ=255) ggTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCt > 2:978802/1‑151 (MQ=255) tttCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGcc > 2:527679/1‑149 (MQ=255) | CAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCC > NC_000913/2846524‑2846822 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |