Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 15,382 | 0 | A | G | 41.8% | 86.0 / 16.1 | 55 | intergenic (+84/‑63) | dnaJ/insL1 | chaperone Hsp40, DnaK co‑chaperone/IS186 transposase |
Reads supporting (aligned to +/- strand): ref base A (4/28); new base G (23/0); total (27/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.40e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGG > NC_000913/15246‑15532 | cAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACC‑‑CCCAt > 2:29782/1‑147 (MQ=255) cAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATaa < 2:778650/149‑1 (MQ=255) aaaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACT‑‑CCCACaa > 1:947915/1‑148 (MQ=255) aaaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAgc < 2:508549/150‑1 (MQ=255) aaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACT‑‑CCCCTAAacg > 1:957883/1‑150 (MQ=255) cttcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCAAT‑‑CCCCTAAtcgccca > 1:93149/1‑146 (MQ=255) tcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACtt < 2:713840/150‑1 (MQ=255) aTGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACT‑‑CCCCTAAACGGCTACTTaaag > 1:260876/1‑146 (MQ=255) aTGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCAAT‑‑CCCAACAAAGCTAACTTAAagg > 2:361294/1‑149 (MQ=255) ggTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTAACG‑‑ACCAAAAAAGCCAACTTAAagg > 1:675468/1‑147 (MQ=255) ggTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑Ttg < 1:573113/150‑1 (MQ=255) gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTgg < 2:458381/150‑1 (MQ=255) gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGt < 2:334650/151‑1 (MQ=255) tgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTAACT‑‑CCCCTAAACGCCAAATTAAAGG‑Gtg > 1:922494/1‑146 (MQ=255) aagaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTAtt < 1:1151739/151‑1 (MQ=255) aagaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTAtt < 1:610628/151‑1 (MQ=255) agaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTa < 1:804984/151‑1 (MQ=255) gaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTAc < 2:976733/151‑1 (MQ=255) gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGatat < 2:716667/150‑1 (MQ=255) gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATg < 1:103884/151‑1 (MQ=255) cgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACT‑‑CCCATAAGCGCTAACTTAAGGG‑GTGTGGTGTTACGCCTGatat > 2:850436/1‑148 (MQ=255) cgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACC‑‑CCCACAAAAGCCAACTTTAGGG‑GTGTggggt > 1:974609/1‑133 (MQ=255) gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACT‑‑CCCCTAAACGCTAACTTTAAGG‑TTGTGGGATTACGCCTGATATGAtt > 2:353995/1‑151 (MQ=255) ccTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACT‑‑CCCATAAGCGCTCAATTAAGGG‑GTGTGGGATTTAGCCTGATATTAttt > 2:185008/1‑150 (MQ=255) cGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTGACT‑‑CCCACAAACGCCAAATTAAAGG‑GTTTGGGATTTCGCCTGATATTATTTTAAGTGcc > 1:614733/1‑151 (MQ=255) gCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCg < 2:370721/151‑1 (MQ=255) tAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACT‑‑TCCATAAAACCTCACCTTAAGG‑TTGTGGGAGTTTGACTTGTATTATTTAACg > 1:1103539/1‑144 (MQ=255) aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt < 1:645778/150‑1 (MQ=255) aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAAGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt < 1:392164/150‑1 (MQ=255) cTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACT‑‑CCCACAAACGCTAACTTAAAGG‑GTGTGGGATTTCGCCCGGTATGATTTTAAGTGCCGGTGaa > 2:960459/1‑150 (MQ=255) ccccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt < 2:1065860/149‑1 (MQ=255) cAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACC‑‑CCCCTAAACGCTAACTTAAGGG‑GTGTGGGATTTCGCCCGGTATTATTTAAAGTGCCGATGaa > 2:1133163/1‑145 (MQ=255) aaGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa < 1:353360/151‑1 (MQ=255) gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACg < 2:426305/151‑1 (MQ=255) gCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAt < 1:772537/149‑1 (MQ=255) cccGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACC‑‑CCCCTAAGCGCCAAATTAAGGG‑GTGTGGGATTAAGCCTGCTATGATTTTAACTGCCGATGGATTATTCTCTCGa > 2:441759/1‑147 (MQ=255) gCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑g < 2:455194/151‑1 (MQ=255) ggCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCaa < 1:950820/151‑1 (MQ=255) aaaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGTACC‑‑CCCCCAAACGGTAACTTTAGGG‑GTGTGGGATTACGCCTGGTATTATTTTAAGAGCCGGTGAATTAATCTCACGATAACTAGGTA‑GGAAATCTTGccc > 1:901103/1‑151 (MQ=255) aaaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGccc < 1:500153/151‑1 (MQ=255) gggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTgg < 1:404161/151‑1 (MQ=255) cGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACT‑‑CCCCTAAGCGCTAACTTAAGGG‑GTGTGGGATTAAGCCTGATATGATTTAAAGTGCCGATGAATTAATCTCACGATAAATGGTCA‑GCAAATCTGGCCCCTATTTGTaa > 2:735729/1‑149 (MQ=255) gTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGAAAA‑‑CCCCTAAAGGCCCACTTAAGGG‑GTGTGGGATTACGGCCGGTATGAATTTACGAGGCGATGAATTACTTTCACCATAAATGGGCA‑Gcgaattttgccc > 1:913395/1‑127 (MQ=255) ttGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGAACTTCCCCAAAAGC‑CTAA‑TTAAGGGTTTTTGGTGTT‑TGGCTGCTATGATTTTAAGGGTCGACGGATTAATCTCCCCCTAACTGGTCAGGCATTTTTGGcccttctggtaaaccc > 1:702764/1‑138 (MQ=255) gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCga < 1:576514/151‑1 (MQ=255) aaGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACT‑‑CCCCTAAACGCCAAATTAAAGG‑GTGTGGGATTTCGGCTTATATTATTTTAAGTGGCGAAGGATTTATCTCCCGAAAAATGGTCA‑GCAATTTTTGCCCCTTTTTGTaaaac > 1:853376/1‑144 (MQ=255) aaGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCgaa < 1:29781/151‑1 (MQ=255) atatGCGAGCACCTGTAAAGTGGCGGGGGACACT‑‑CCCCTAAACGCTAACTTAAGGG‑GTGTGGGGTTATTCCTGGTATGATTTTTAGTGCCGATGAATTAATCCCCCGCTAAATCGTCA‑GCAAATCTTGCCCATATTTGTAAGacc > 1:68442/1‑145 (MQ=255) gAGCACCTGTAAAGTGGCGGGGAGTACT‑‑CCCCTAAGAGCCTACTTTAGGG‑GTGGGGGGTTTCGCCCGATATGATTTTAAGTGCCGATGAAATACTCTCACGATAAATGGTCA‑GCAATTCTTGGCCCTATTTGTAAACCCCAAGAACTgg > 1:50244/1‑149 (MQ=255) ccTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTc < 2:922517/151‑1 (MQ=255) gTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCg < 2:110215/149‑1 (MQ=40) gCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATg < 2:469893/151‑1 (MQ=34) cGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGc > 2:228162/1‑151 (MQ=33) ggggATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGcc > 2:1146305/1‑151 (MQ=33) ggggATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGc > 2:60363/1‑150 (MQ=33) aTCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCgggg > 1:428780/1‑151 (MQ=33) | CAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACT‑‑CCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCA‑GCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGG > NC_000913/15246‑15532 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |