Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	47.4%	75.0 / 19.9	57	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/25);  new base G (27/0);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 2.32e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437                                                                                                                                                    |                                                                                                                                                      ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaa                                                                                                                                                    <  2:1024842/151‑1 (MQ=255)    gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                 <  1:953098/151‑1 (MQ=255)     cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGaa                                                                                                                                                    >  2:857845/1‑147 (MQ=255)      aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATg                                                                                                                                               <  1:990049/151‑1 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                                                                             <  2:647070/151‑1 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGaag                                                                                                                                               >  1:853993/1‑145 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTg                                                                                                                                          >  2:848810/1‑150 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATg                                                                                                                                               >  2:16263/1‑145 (MQ=255)             aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                        <  2:500483/151‑1 (MQ=255)                     ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAgg                                                                                                                                <  1:343705/151‑1 (MQ=255)                      gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                               <  1:318637/151‑1 (MQ=255)                       ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTTGGGGGAAAGATGCGCTggagg                                                                                                                                      >  1:334208/1‑139 (MQ=255)                       ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCACGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTGAAAGGGCAgggg                                                                                                                              >  1:396079/1‑149 (MQ=255)                        tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGTGGAAGAAGGGCCGGAAGGGGAggggg                                                                                                                             >  1:947435/1‑151 (MQ=255)                           cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAggtggggg                                                                                                                          >  2:265529/1‑151 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAgggggcggggg                                                                                                                       >  2:52215/1‑151 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                                      <  2:227629/150‑1 (MQ=255)                                 aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGt                                                                                                                    <  1:832233/151‑1 (MQ=255)                                    ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCGGCAAGGGGAGGGGGCGGGGGCGgtt                                                                                                                  >  1:1004890/1‑150 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGGAAGATGGGCTGAAAGGGGAGGGGGGGGGGGCGTTTGt                                                                                                                >  2:714868/1‑150 (MQ=255)                                         gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGAAGGGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCAtt                                                                                                            >  1:207212/1‑151 (MQ=255)                                             aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAAGGGAgggggcgggggggggtttcgttc                                                                                                           >  1:1077925/1‑136 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                      <  2:75356/150‑1 (MQ=255)                                                     tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATGGGCTGGAAGGGCGGGAGGCGGTGGGGTTTGTCATTCCGGAagag                                                                                                   >  2:686645/1‑148 (MQ=255)                                                        aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAagaggg                                                                                                 >  1:450715/1‑145 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                     <  2:157620/150‑1 (MQ=255)                                                                      ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                               <  1:629993/151‑1 (MQ=255)                                                                          tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa                                                                            <  1:30079/150‑1 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                                           <  2:770174/150‑1 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGAt                                                                           >  1:404731/1‑150 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTTCGAAAGAGAGCGAGAGTCTGGAAGATCGTGATGTgg                                                                       >  1:1140948/1‑150 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                       <  2:441284/149‑1 (MQ=255)                                                                                   gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc                                                                  >  2:1109714/1‑151 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               <  1:821273/151‑1 (MQ=255)                                                                                        gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGGTGTGGCGCACTCGc                                                              >  1:451248/1‑150 (MQ=255)                                                                                         cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGTGAAATAAGTGCTGAAAAGGCAGGTGGGGGTGGCGCTTTTGCTTCCGAAAGAGAGCCAAAGACTCGAAGGACGAGATGGGgcgacc                                                                  >  2:42471/1‑142 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAACGGGCAGGGGGCGGGGGGGGTTGTTCTTCCCAAAGAGAGCGACAGTCTGGAAGAACGGGATGTGGGGCCCTc                                                                >  2:689879/1‑144 (MQ=255)                                                                                              ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGGGCTGAAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                        >  2:621654/1‑150 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTTGTTGGGGGGCAACAAGCGCTGAAAAGGCCGGGGGGGGGGGCGTTTGTCCTTTCGGAAGAGGGCGAAAGTTTTGAAGATCGTGGTGTGGGGCCCCCGCAag                                                           >  1:273556/1‑146 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                       <  2:139356/150‑1 (MQ=255)                                                                                                gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  1:745655/150‑1 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCTGGAAGGGCCGGGGGCGGGGGCGGTTGGCATTTCGGAAGAGAGGGGAAGTCTGGAAGGTCGTGGTGTGGGGCACTCGCAAGAGAAGGCg                                                   >  1:624338/1‑151 (MQ=255)                                                                                                       gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                              <  1:517211/151‑1 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGAAAGATGCGCTGAAAGGGGCGGGGGGGGGGGCGGTTGGCATTCCGAAAGAGAGCGACAGACTGGAAGATCGTGATGGGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                         >  1:51389/1‑150 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGGGGGGCACTCGCAAGAGAAGGCGGTTATGGCGCtgg                                      >  2:162664/1‑151 (MQ=255)                                                                                                                gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                     <  2:8063/151‑1 (MQ=255)                                                                                                                gCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                      >  2:527446/1‑150 (MQ=255)                                                                                                                gCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCCGAAAGGGCCGGGGGCGGGGGGGTTTGTGATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGATTATGGCGCggg                                      >  1:115630/1‑150 (MQ=255)                                                                                                                 ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAAGCGATTATGGCGCtggtg                                    >  1:1145040/1‑151 (MQ=255)                                                                                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                                 <  1:9964/150‑1 (MQ=255)                                                                                                                      cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   >  1:401491/1‑147 (MQ=255)                                                                                                                                 gAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACCCGCAAGAGAAGGCGGTTATGGGGCTGGTGGGCAGCCAGATTgg                      >  1:28651/1‑149 (MQ=255)                                                                                                                                  aaGTGGCGGTGGTTGGGGGGACAGATGCGCTGAAAGGGCAGGTGGGCGGGGCGTTTGTCAATCCGCAAGCGAGCTCTAGTCTGGAAGATCGAGCAGCTGCGCCATCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCTAGATTggga                    >  1:1100342/1‑148 (MQ=255)                                                                                                                                      ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt               <  1:139474/151‑1 (MQ=255)                                                                                                                                         ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg             <  2:360885/150‑1 (MQ=255)                                                                                                                                            ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg          <  2:637353/150‑1 (MQ=255)                                                                                                                                                  ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg   <  1:604593/151‑1 (MQ=255)                                                                                                                                                    tGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  1:25827/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑