Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 233,963 (GT)4→5 intergenic (‑8/+64) mltD ← / ← gloB putative membrane‑bound lytic murein transglycosylase D/hydroxyacylglutathione hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913233,9541.T83.6% 214.6 / 33.1 61coding (2/1359 nt)mltDputative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (6/4);  new base T (21/30);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01
*NC_000913233,9542.G83.6% 206.2 / 35.1 61coding (2/1359 nt)mltDputative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (6/4);  new base G (21/30);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01

CCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑TGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTT  >  NC_000913/233806‑234094
                                                                                                                                                     ||                                                                                                                                              
cccATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑t                                                                                                                                               <  2:918490/150‑1 (MQ=255)
     cGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑tgtgtgt                                                                                                                                         <  2:602206/151‑1 (MQ=255)
      gTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑tgtgtgtg                                                                                                                                        <  1:509966/151‑1 (MQ=255)
        ccatccatCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtg                                                                                                                                      >  2:624185/1‑150 (MQ=255)
        ccatccatCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtg                                                                                                                                      <  2:964947/151‑2 (MQ=255)
         catccatcGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtgt                                                                                                                                     >  2:982594/1‑149 (MQ=255)
          atccatcGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtgtc                                                                                                                                    <  2:71371/151‑4 (MQ=255)
          atccatcGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtgtc                                                                                                                                    >  2:80803/1‑148 (MQ=255)
           tccatcGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtgtc                                                                                                                                    >  1:668145/1‑147 (MQ=255)
           tccatcGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtgtc                                                                                                                                    >  1:941931/1‑147 (MQ=255)
              atcGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtgtcaata                                                                                                                                <  1:456885/151‑8 (MQ=255)
                cGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑Tgtgtgtgtgtcaatagt                                                                                                                              >  1:623967/1‑142 (MQ=255)
                       gACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTa                                                                                                                        <  1:35759/150‑1 (MQ=255)
                         cTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTaaa                                                                                                                      >  2:432949/1‑150 (MQ=255)
                          ttGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTaaa                                                                                                                      >  1:649490/1‑149 (MQ=255)
                              aaaCTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAgacga                                                                                                                <  1:183644/151‑1 (MQ=255)
                              aaaCTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGATACTCTGGCATCCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAgtcga                                                                                                                <  1:183650/151‑1 (MQ=255)
                               aaCTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAgacgac                                                                                                               <  1:812679/151‑1 (MQ=255)
                               aaCTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAgacgac                                                                                                               >  1:188939/1‑151 (MQ=255)
                                        gctTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATaa                                                                                                      <  2:234234/151‑1 (MQ=255)
                                        gctTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATa                                                                                                       >  1:283692/1‑150 (MQ=255)
                                          ttCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACg                                                                                                    >  2:357889/1‑151 (MQ=255)
                                           tCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAAc                                                                                                     <  1:382484/149‑1 (MQ=255)
                                           tCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACg                                                                                                    <  2:760920/150‑1 (MQ=255)
                                            ccccTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACgg                                                                                                   <  1:432304/150‑1 (MQ=255)
                                            ccccTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGc                                                                                                  <  2:997998/151‑1 (MQ=255)
                                                   aCCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGa                                                                                           <  1:240574/151‑1 (MQ=255)
                                                      aGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGcc                                                                                        >  2:1017292/1‑151 (MQ=255)
                                                        cTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCg                                                                                       >  2:449463/1‑150 (MQ=255)
                                                        cTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCg                                                                                       <  2:18826/150‑1 (MQ=255)
                                                                  gCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGaaa                                                                            <  2:435387/151‑1 (MQ=255)
                                                                  gCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGaa                                                                             >  1:208854/1‑150 (MQ=255)
                                                                         gcgtgcTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTAt                                                                     <  2:435918/151‑1 (MQ=255)
                                                                         gcgtgcTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTAt                                                                     <  2:284117/151‑1 (MQ=255)
                                                                         gcgtgcTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTAt                                                                     <  2:427324/151‑1 (MQ=255)
                                                                          cgtgcTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATc                                                                    <  1:955365/151‑1 (MQ=255)
                                                                             gcTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGa                                                                 <  1:170107/151‑1 (MQ=255)
                                                                                gTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAAcc                                                              <  2:393342/151‑1 (MQ=255)
                                                                                  tGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCt                                                             <  2:759276/150‑1 (MQ=255)
                                                                                   gAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTAt                                                           <  1:110764/151‑1 (MQ=255)
                                                                                   gAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTAt                                                           <  2:131071/151‑1 (MQ=255)
                                                                                   gAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTAt                                                           >  2:18426/1‑151 (MQ=255)
                                                                                     aCGTTGCCGGTACTCTGGCGACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTAtct                                                         <  2:825188/151‑1 (MQ=255)
                                                                                                    tGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAAc                                          >  2:746372/1‑151 (MQ=255)
                                                                                                       cAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCAt                                       <  2:1004087/151‑1 (MQ=255)
                                                                                                        aaCCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCa                                        >  2:957436/1‑149 (MQ=255)
                                                                                                        aaCCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATg                                      >  1:208206/1‑151 (MQ=255)
                                                                                                           ccACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCa                                    >  1:266116/1‑150 (MQ=255)
                                                                                                               gAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACg                               >  2:780234/1‑151 (MQ=255)
                                                                                                                   aGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGctct                           <  2:286428/151‑1 (MQ=255)
                                                                                                                   aGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGctct                           >  1:273667/1‑151 (MQ=255)
                                                                                                                   aGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGctct                           <  2:188934/151‑1 (MQ=255)
                                                                                                                    ggACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCtt                          <  1:807174/151‑1 (MQ=255)
                                                                                                                    ggACAGAGGCGAGTAATATCGCTTGTGCCTTCA‑‑TGTGTGTGTTGCAATAGTTGCTTAAAAGCCGACCGATCATAACGGCGAACGTAGCCGATGACAAGAAAGGTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGct                             <  2:693920/148‑1 (MQ=255)
                                                                                                                       cAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTc                         >  2:28586/1‑149 (MQ=255)
                                                                                                                       cAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTc                         >  2:697809/1‑149 (MQ=255)
                                                                                                                       cAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTc                         >  2:685046/1‑149 (MQ=255)
                                                                                                                       cAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTc                         >  1:931224/1‑149 (MQ=255)
                                                                                                                          agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGgtt                    <  2:701110/151‑1 (MQ=255)
                                                                                                                             cGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGgttgtt                 >  1:552609/1‑151 (MQ=255)
                                                                                                                              gAGTAATATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGttgttg                >  1:54965/1‑151 (MQ=255)
                                                                                                                                  aaTATCGCTTTTGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCaat            <  1:655738/151‑1 (MQ=255)
                                                                                                                                           tttGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGttt     <  2:898695/149‑1 (MQ=255)
                                                                                                                                             tGCCTTCATGTGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTttctt  <  2:703208/150‑1 (MQ=255)
                                                                                                                                                     ||                                                                                                                                              
CCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTGCAGAAAGGCTCTGTGCGTGCTGTTGAACGTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑TGTGTGTGT‑‑CAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTT  >  NC_000913/233806‑234094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: