Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,079,4680AG43.6% 88.4 / 14.2 55D55G (GAT→GGT) putPproline:sodium symporter
Reads supporting (aligned to +/- strand):  ref base A (3/28);  new base G (24/0);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.18e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAAC  >  NC_000913/1079323‑1079616
                                                                                                                                                 |                                                                                                                                                             
aTGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGa                                                                                                                                                         <  1:828671/151‑1 (MQ=255)
aTGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGgtat                                                                                                                                                           >  1:971465/1‑149 (MQ=255)
  gTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGc                                                                                                                                                      <  1:225475/151‑1 (MQ=255)
  gTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGc                                                                                                                                                      <  1:230207/151‑1 (MQ=255)
  gTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGc                                                                                                                                                      <  2:243615/151‑1 (MQ=255)
  gTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGTGGGTATgggg                                                                                                                                                       >  1:740359/1‑148 (MQ=255)
      gTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCggct                                                                                                                                                 <  1:598288/151‑1 (MQ=255)
            ttttGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGGGATGAGCGggtgggtg                                                                                                                                           >  2:338878/1‑150 (MQ=255)
              ttGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTGGGGGATGAGGGGGCGGGTGttt                                                                                                                                       >  1:648254/1‑150 (MQ=255)
                gtgtCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAAt                                                                                                                                     <  1:342084/151‑1 (MQ=255)
                   tCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGGTGGGTGTTTAt                                                                                                                                     >  1:797416/1‑148 (MQ=255)
                      atatCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATggg                                                                                                                                  <  2:810640/148‑1 (MQ=255)
                                 aTGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTg                                                                                                                  <  1:837534/151‑1 (MQ=255)
                                   gATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCATAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATGAGCGGCTGGCTGTTTATGGGGGTGCCGGgcgccg                                                                                                                   >  1:913181/1‑147 (MQ=255)
                                    atatTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGttt                                                                                                              <  2:245953/151‑1 (MQ=255)
                                      attgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGGCGGGTATGAGCGGGCGGGTGTTTATGGGGGTGCCGGGGGCTGgtttt                                                                                                            >  1:117764/1‑151 (MQ=255)
                                               gTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCggg                                                                                                   <  2:474180/151‑1 (MQ=255)
                                               gTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCggg                                                                                                   <  2:193086/151‑1 (MQ=255)
                                               gTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCggg                                                                                                   <  2:596169/151‑1 (MQ=255)
                                                 ttATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGa                                                                                                  <  1:456525/150‑1 (MQ=255)
                                                 ttATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGa                                                                                                  <  2:655981/150‑1 (MQ=255)
                                                 ttATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTTTGAGAGGGCGGCGGTTTATTGGGGTGGCGGGGGCTGTttttttttt                                                                                                       >  2:695487/1‑144 (MQ=255)
                                                     cGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTcc                                                                                             <  1:614944/151‑1 (MQ=255)
                                                     cGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTc                                                                                              <  2:52316/150‑1 (MQ=255)
                                                       ccTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGGGGGGTGTTTATTGGGGTTGCGGGGGCCGTTTTTTTTTTCCGGGGtt                                                                                                >  1:954705/1‑147 (MQ=255)
                                                         tGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGGTATGAGGGGCGGGGTGTTTATGGGGGTGGCGGGGGCTGTTTTTTTTTTCTGGGTTTTCgca                                                                                          >  1:85428/1‑148 (MQ=255)
                                                         tGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGGGGGGATTGGGGGGCGGGGGTTTATTGGGGTGCCGGGGGGCGGTTTTTTTTTCGGGGGTTTcca                                                                                            >  2:478259/1‑148 (MQ=255)
                                                             gATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTg                                                                                    <  1:731406/151‑1 (MQ=255)
                                                              aTCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGAGGGTCGGGTATGAGCGGGGGGCGGGTTATTGGGGTGGCCGGCGCTGTTTTTTTTTTCGGGGTTTTCCGaaaatgg                                                                                     >  1:741913/1‑147 (MQ=255)
                                                               tCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTGGGAGCGGGTGGGTGGTTTTTGGGGTGGGGGGGGCTGGTTTTTTTTTTCGGGttttgccaagatgg                                                                                     >  2:438002/1‑140 (MQ=255)
                                                                cAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGAt                                                                                 <  1:240009/151‑1 (MQ=255)
                                                                 aaCGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGTCGGGTATTAGCGGGGGGGTGTTTATGGGGGTGTCGGGGGGGGTTTTTCTTTTCGGGGTTTCCGgaaacgtg                                                                                    >  2:373982/1‑143 (MQ=255)
                                                                   cGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGc                                                                              <  2:155350/151‑1 (MQ=255)
                                                                      aaaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGCGGCTGTTAATTGGGGTGCCGGGGGGTGTTTTTTTTTTCTGGGGTTCCTAAAGCTGGATCGcc                                                                             >  2:463582/1‑149 (MQ=255)
                                                                       aaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGGGGGTGGGTGTTTATGGGGTGGGCGGGGGGCGTTTTTTTTTTCCGGGTTTTCCGaaactgggtggcc                                                                               >  1:865003/1‑138 (MQ=255)
                                                                       aaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGCGGCTGGCTGTTTATGGGGGTTGCGGGGGCTGTTTTTTTTTTCGGGGTTTTCGAAAGCTGGATCGCCAtt                                                                          >  1:947991/1‑151 (MQ=255)
                                                                        aaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTg                                                                         <  2:602102/151‑1 (MQ=255)
                                                                        aaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTg                                                                         <  1:192074/151‑1 (MQ=255)
                                                                            ttGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATTGGGGGGTGGGGGGTTTTTGGGGTGGCGGGGGGTGGTTTTTTTTTCGGGGGTTTCGGaaacggggaaggcct                                                                             >  2:634926/1‑133 (MQ=255)
                                                                            ttGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATGAGGGGCGGGGCGTTTATTGGGGTGGCCGGGGCCGTTTTTTTTTTTCGGGGTTTCCTAAGCTGGGTCGcccct                                                                          >  2:896051/1‑143 (MQ=255)
                                                                                    tataTTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGGGGGTATGAGGGGGTGGGTGTTTATGGGGGTGGCGGGGGCTGTTTTTTTTTTTGGGGGTTCCGTAAGATGGGTGGCCACTTGCCTGTCAtt                                                               >  2:579334/1‑149 (MQ=255)
                                                                                     atatTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTa                                                              <  2:524310/149‑1 (MQ=255)
                                                                                      tatTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGTGGGTGTTTATGGGGTTGGCGGGCGGTGTTTTTTTTTTCGGGGTTTTCGAAAGCTGGATCGCCATTGGCCTCTCAtt                                                               >  1:26015/1‑147 (MQ=255)
                                                                                           tGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGGGGGCATGAGCGGGTGGGGCTGTTTGGGGGGGGCGGGCGCGGTTTTTTTTTTTGGGGGTTTCTAAAGTCGGGATGCCCATGGGCCGACATTatgg                                                           >  2:897205/1‑143 (MQ=255)
                                                                                                 gTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTaa                                                <  1:111101/151‑1 (MQ=255)
                                                                                                    gTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTg                                             <  2:608038/151‑1 (MQ=255)
                                                                                                       gTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGGATGAGGGGGTGGCTGTTAATGGGGGGGTCGGGGGCGGTTTTTTTTTTCCGGGTTTTCGTAAGATGGGTCGCCCCTGGCCTGCCATTTGGGGCGGGGGtt                                                  >  1:319586/1‑143 (MQ=255)
                                                                                                        tCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGGTTTGAGGGGGCGGGTGGTTATTGGGGTGGCGGGGGCGGGTTTTTTTTTTTGGGTTTTCTCAAAATGGGTCGCCCTTTGGCTCTCATTTGGCGCGGGGGtttaa                                               >  2:334498/1‑142 (MQ=255)
                                                                                                            gggCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATGAGGGGCGGGGTGGTTATGGGGGTGCCGGGGGGTGGTTTTTTTTTCGGGGGTTTCGAAAACTGGGTCGCCCTTGGGCTGACATTTTGCGCGTGGGTTTAATGGGAGGtg                                      >  1:612957/1‑150 (MQ=255)
                                                                                                                    cGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCggg                             <  2:648238/151‑1 (MQ=255)
                                                                                                                     gTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGc                            <  1:403860/151‑1 (MQ=255)
                                                                                                                       gACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCg                           <  1:319835/150‑1 (MQ=255)
                                                                                                                                   ggcggGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCAt              >  2:696901/1‑151 (MQ=255)
                                                                                                                                      gggTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATAcc           >  2:19921/1‑151 (MQ=255)
                                                                                                                                               ggATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATacaac  <  2:117760/151‑1 (MQ=255)
                                                                                                                                                gATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATacaac  >  1:377982/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                             
ATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAAC  >  NC_000913/1079323‑1079616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: