Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA46.8% 48.7 / 26.2 47intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (20/5);  new base A (0/22);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 9.41e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824906‑1825205
                                                                                                                                                        |                                                                                                                                                     
aCCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagag                                                                                                                                                       <  1:871328/151‑1 (MQ=255)
   gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagagaa                                                                                                                                                     >  1:685124/1‑150 (MQ=255)
     cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                                  <  1:719722/151‑1 (MQ=255)
     cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                    >  2:578916/1‑149 (MQ=255)
         ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                               <  2:703330/150‑1 (MQ=255)
           aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                            <  1:94178/151‑1 (MQ=255)
            cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                           <  1:840264/151‑1 (MQ=255)
                 atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACCGGGGTGATTTACGTTAGGGGTGATCAGTCTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAAccgccg                                                                                                                                        <  1:449645/149‑1 (MQ=255)
                  tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                                     >  2:953827/1‑151 (MQ=255)
                     aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                  >  2:828643/1‑151 (MQ=255)
                       gTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                                                >  2:366233/1‑151 (MQ=255)
                              aTACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                          >  2:469755/1‑150 (MQ=255)
                              aTACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                          >  1:241498/1‑150 (MQ=255)
                                aCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                       >  2:336204/1‑151 (MQ=255)
                                 cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                       >  1:737178/1‑150 (MQ=255)
                                   cGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                    >  2:502235/1‑151 (MQ=255)
                                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   >  2:137643/1‑151 (MQ=255)
                                        ccGGCCCGTTTTTTTATTTTTTATCGTTCAGGTACCGGTGGATGTTTATCGCCAACTGGGGTGATTTACGTTAGGGGTGATCGGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAg                                                                                                               <  1:756919/151‑1 (MQ=255)
                                          ggCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                             >  1:299265/1‑151 (MQ=255)
                                            cccTTTTTTTTCATTTTTATCTTCCAGGTACCCGTGGATGGTTATCGCCAACTCGGTTAATTTACTTTAGGGGTAACCTGTCTTTTTTTACACCTGTTTAAAAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg                                                                                                           <  2:90639/151‑1 (MQ=255)
                                            cccGTTTTTTTCTTTTTTATCTTTCAGGTAACCGGGGATGTTTATCGGCAACTGGGTTTTTTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTGTTTAAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTa                                                                                                            <  1:635570/150‑1 (MQ=255)
                                              cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                         >  2:542296/1‑151 (MQ=255)
                                              cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                         >  1:973241/1‑151 (MQ=255)
                                                    tttCATTGTTATCGTTCAGGTAACCGGGGTTGGTTATCGGCAACTGGGGTGATTTACGTTAGGGGTGATCGGTTTTTTTTTACACTTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                      <  2:829494/148‑1 (MQ=255)
                                                        cttGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                               >  2:602366/2‑151 (MQ=255)
                                                          ttttATCTTTCAGGTACCGTGGGGTGGTTTGCGGCCACTGGGGGGTTTTCCTTTAGGGGGGACCACTTTTTTTTAACAACTATTGGAAAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                              <  2:575437/148‑1 (MQ=255)
                                                                  ttgggttAACCGTGGATGGTAGTCGGCACGTGGGGTATTTAACGTGGGTGTTTATGTGGCTTTTTTTCCACTTGTGAGAGAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                      <  1:431032/144‑1 (MQ=255)
                                                                  ttcggttAACGGTGGTTGTTTATGGGCAACTCGGGTGATTTACTTAAGGGGTGATCAGGTTTTTTTTACACCTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                       <  2:971720/143‑1 (MQ=255)
                                                                      gttagccGGGGATGGTTATCGGCACCTGGGGTTTTTTACGTTAGTGGTGATCAGGTTTTTTTAACAACTTTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                        <  1:782270/139‑1 (MQ=255)
                                                                      ggTAACGGGGGATGGTTACCGGCAACTGGGTTAATTTACGTGGGTGGTGACCGGGTTTTTTTTACACTTTTTTGAGAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                                   <  2:395511/149‑1 (MQ=255)
                                                                      ggTAACCGGGGAGGTTTACCGGAAACTGGGTTGTTTTACGTTAGTGGTAATCGGTTTTTTTTAACACTTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                  <  2:976047/150‑1 (MQ=255)
                                                                         agccGTGGATGGTTATGGACAACTCGGGTAATTTACGTTAGTGGTGACCAGGCTTTTTTCAAAACTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                  <  1:846636/145‑1 (MQ=255)
                                                                         aacgttGGATGGTTATCGAAAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt                                                                               <  2:790259/145‑1 (MQ=255)
                                                                          accgggggATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCGGGTTTTTTTAACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt                                                                               <  2:897400/144‑1 (MQ=255)
                                                                           ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                              >  1:907109/1‑149 (MQ=255)
                                                                                 gATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                         >  2:441395/1‑149 (MQ=255)
                                                                                           ggaacctggggTGTTTTACGTTGGTGGTGACCAGGTTTTTTTTACAATTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                              <  1:787722/142‑1 (MQ=255)
                                                                                           ggCAACTGGGGTGTTTTCCGTTAGTGGTGATCAGTTTTTTTTTAAAACTGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                               <  1:9436/149‑1 (MQ=255)
                                                                                              aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                           >  1:229548/1‑150 (MQ=255)
                                                                                                   gggTGTTTTGCTTTGGTGGTAATCAGGTTTTTTTTACAACTTTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                     <  1:756912/151‑1 (MQ=255)
                                                                                                               taaGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                            <  1:168311/146‑1 (MQ=255)
                                                                                                                    ggtgATCAGGTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAcc                                         <  1:183998/146‑1 (MQ=255)
                                                                                                                             gtttttttttAAACTTTTTGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                              <  1:786022/147‑1 (MQ=255)
                                                                                                                               ttttttaACAGTTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAc                           <  1:83172/149‑1 (MQ=255)
                                                                                                                                ttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                        >  2:500027/1‑151 (MQ=255)
                                                                                                                                    ttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCt                      >  1:825899/1‑149 (MQ=255)
                                                                                                                                      acacttGTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTg                     <  2:737157/143‑1 (MQ=255)
                                                                                                                                         aCTGTTGGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                  <  1:828663/148‑1 (MQ=255)
                                                                                                                                           tGTTTAAGAAAAGAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                   <  1:155173/145‑1 (MQ=255)
                                                                                                                                            gTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt            >  1:220129/1‑151 (MQ=255)
                                                                                                                                             tttGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg              <  2:374470/148‑1 (MQ=255)
                                                                                                                                             cttGAGACGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCCTTAGTTTTTTGGCTGAAAGTTTTGTCAGCAATTGCACGCGCTTTTCCTTTTGCCACTGGCCGGTTTGTCAAAc               >  2:43853/2‑147 (MQ=255)
                                                                                                                                                  agaaaaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa       <  1:428859/151‑1 (MQ=255)
                                                                                                                                                   tttgagaaGAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa       <  2:753406/147‑1 (MQ=255)
                                                                                                                                                    aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa       <  2:658561/149‑1 (MQ=255)
                                                                                                                                                        gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt  >  2:643507/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824906‑1825205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: