Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091315,4100TG42.5% 71.2 / 11.6 47intergenic (+112/‑35)dnaJ/insL1chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
Reads supporting (aligned to +/- strand):  ref base T (5/22);  new base G (20/0);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 9.41e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT  >  NC_000913/15261‑15536
                                                                                                                                                        |                                                                                                                              
aTGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGtt                                                                                                                               <  1:450090/151‑1 (MQ=255)
     gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGt                                                                                                                          <  2:334212/151‑1 (MQ=255)
       gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                                                                                                         <  2:940305/150‑1 (MQ=255)
       gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                                                                        <  2:974152/151‑1 (MQ=255)
         agaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCAACCCCCTAAACGCTAAACTAAAGGGtggggggtt                                                                                                                        >  2:928789/1‑142 (MQ=255)
          gaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGACAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCCATTCCCTAAACGCCTAATTTAAGGGtgtgg                                                                                                                            >  1:854883/1‑141 (MQ=255)
               tttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTGCGCCt                                                                                                                 <  2:589327/150‑1 (MQ=255)
               tttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAACACTCCCCCAAAAGCCCACTTAAGGGGTGTGGGATTATGCCt                                                                                                                 >  1:617834/1‑150 (MQ=255)
                 tttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACCCCCCTAAACGCTTAATTAAGGGGGGTggggtttagcccgtt                                                                                                              >  1:895848/1‑138 (MQ=255)
                       gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACCCCCCTCAACGCCACATTTAGGGGTGTGGGATTAAGCCTGTTATGa                                                                                                          >  2:712948/1‑149 (MQ=255)
                          cTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGGTAACTTAAGGGGTGGGGTGTTAAGCCTGattttat                                                                                                         >  2:839839/1‑142 (MQ=255)
                            gACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACg                                                                                                   <  2:309300/151‑1 (MQ=255)
                                cGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGACACCCCCCCAAGCGCTAACTTTAGGGGTGTGGGGTTATGCCCGATATGAttt                                                                                                       >  1:9738/1‑143 (MQ=255)
                                 gCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCg                                                                                              <  1:993872/151‑1 (MQ=255)
                                  cTAACCTCCCCAAAAGCCTTCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCg                                                                                              <  2:721125/150‑1 (MQ=255)
                                    aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt                                                                                            <  1:834486/150‑1 (MQ=255)
                                     aCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGATACTCCCATAAACGCTAAATTTAAGGGTGTGGGGTTACGCCTGATATGATTTTAAGTGCCGAt                                                                                            >  1:680961/1‑149 (MQ=255)
                                     aCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGa                                                                                          <  2:1006380/151‑1 (MQ=255)
                                         ccccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCATAAACGCGAACTTAAGGGGTGTGGGATTTCGCCTGGTATGATTTTAAGTGCCGGTg                                                                                           >  2:167739/1‑146 (MQ=255)
                                         ccccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTa                                                                                      <  1:723505/151‑1 (MQ=255)
                                          cccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCTATGAATTAc                                                                                     <  2:305630/151‑1 (MQ=255)
                                           ccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                                    <  2:948297/151‑1 (MQ=255)
                                           ccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                                    <  1:318493/151‑1 (MQ=255)
                                                   cTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACTCCCCTCAAACCCAAACTTTGGGGTGTGGGGTTAAGCCCGGTATGATTTAAAAGGCCGATGAATTAATCTCCCggt                                                                            >  2:658576/1‑149 (MQ=255)
                                                   cTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCCCAAACGCCAAATTTAGGGGTGTGGGATTACGCCTGGTATGATTTTAAGTGCCGATGAAATACTCTCACg                                                                              >  1:171782/1‑149 (MQ=255)
                                                        cGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCATAAGAGCTCACTTAAGGGGTGTGGTATTAATCCTGAGTTTATTTTAATTGCCGATGAATTTcttaccagcaaactg                                                                       >  2:908104/1‑138 (MQ=255)
                                                        cGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTg                                                                       <  1:886747/151‑1 (MQ=255)
                                                           gggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACCCCCCTAAACGCCAACTTAAGGGGTGTGGGATTACGCCCGATATGATTTTACGTGCCGATGAATTACTCTCACGCTAAATgg                                                                      >  1:578755/1‑149 (MQ=25)
                                                              cAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCCAAACGCCAACTTTAGGGGTGTGGGATTTTGCCCGATATGATTTAAAGTGCCGCTGAAATAATCTCACGATAAATGGTCAGc                                                                 >  1:893874/1‑151 (MQ=255)
                                                                ggCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCaa                                                               <  2:771742/151‑1 (MQ=255)
                                                                  ccTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAACGCTAAATTTAGGGGTGTGGGATTAAGCCCGATATGATTTTACGTGCCGGTGAATTAATCTCACCATAACTGGGCAGCAAtt                                                             >  1:173395/1‑151 (MQ=255)
                                                                   cTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAGCGCCAACTTAAGGGGTGTGGGATTACGCCTGATATGATTTTAAGTGCCGATGAATTACTCTCACGATAAATGGTCAGCaaat                                                             >  1:882135/1‑148 (MQ=21)
                                                                         aaaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAAAGCCAACTTAAAGGGTGTGGGATTTTGCCCGATTTGATTTTAAGTGCCGGTGAATTATTCTCACCATAAATTGTCAGCCATTTTTGccc                                                      >  2:358896/1‑151 (MQ=255)
                                                                              tAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGGCAACTTAAGGGTTGTGGTGTTTTGCCTGGTTTGATTTTACGTGCCGGTGAATTAATCTCACCATAAATGGTCAGGAATTCTTGCCCCTAtt                                                 >  2:465186/1‑151 (MQ=255)
                                                                                gggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCCAACTTTAAGGGTGTGGTATTACGCCTGATATGATTTAAAGTGCCGATGAATTAATCTCACGATAACTTGGCAGCAATTCTTGCCCATAtt                                                 >  1:883846/1‑149 (MQ=18)
                                                                                    gCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTaa                                            <  1:920626/150‑1 (MQ=255)
                                                                                       ttGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGAACTCCCATAAACGCTAAATTAAGGGGTGTGGGATTACGCCCGATATGATTTAAAGTGCCGGTGAATTAATCTCAAGATAAATGGGCAGCAAATCTGGCCCCTATTTGTAAGacc                                        >  1:606002/1‑151 (MQ=255)
                                                                                         gAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAAAGCCAAATTAAGGGGTGTGGGGTTATGCCTGATATGTTTTAAAGTGCCGAGGAAATACTCTCACGACAACAGGTCAGCAATTCTGGCCCCTATTGGTAAGccc                                        >  2:504434/1‑149 (MQ=255)
                                                                                          aaGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaa                                     <  1:560668/151‑1 (MQ=255)
                                                                                            gATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaaga                                   <  1:637032/151‑1 (MQ=255)
                                                                                              tatGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAGCGCTAACTTTAGGGGTGTGGGGTTACGCCTGGTATGATTTTAAGTGCCGATGAATTACTCTCACGATAAATGGGCAGCAAATCTTGGCCCTATTGGTAAGCCCgaagaa                                  >  1:59678/1‑150 (MQ=17)
                                                                                               atGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCATAAACGCTAACTTTAAGGGTGGGGTATTTAGCCCGGTATGATTTTAAGTGCCGATGAATTATTCTCCCGATAAATGGTCAGCAATTCTTGCCCCTATTTGTAAGCCCgaagaa                                  >  1:700356/1‑149 (MQ=255)
                                                                                                        ccTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTc                       <  2:171777/151‑1 (MQ=255)
                                                                                                             aaaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCa                   <  2:237405/150‑1 (MQ=39)
                                                                                                              aaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACg                 <  2:179550/151‑1 (MQ=38)
                                                                                                                 tGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTa               >  2:85608/1‑150 (MQ=35)
                                                                                                                      gggATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCg         <  2:866597/151‑1 (MQ=33)
                                                                                                                         aTCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCgggg      >  2:123172/1‑151 (MQ=33)
                                                                                                                             cTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGctct  >  2:145919/1‑151 (MQ=11)
                                                                                                                             cTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGctct  >  1:621799/1‑151 (MQ=11)
                                                                                                                                                        |                                                                                                                              
ATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT  >  NC_000913/15261‑15536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: