Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,131,440 | 0 | T | C | 41.4% | 94.9 / 14.5 | 58 | G323G (GGA→GGG) | yghQ | putative inner membrane polysaccharide flippase |
Reads supporting (aligned to +/- strand): ref base T (29/5); new base C (0/24); total (29/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.85e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCG > NC_000913/3131291‑3131578 | gaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTc > 1:514789/1‑151 (MQ=255) gaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTc < 2:302165/151‑1 (MQ=255) aaatcaCTGAGTCAGCCGAGAAGAATTTTCCCGCTTATTCGCTCCTTCCCTAAATCAGCTCATACGCTTCGAGATACTTACCGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTcc < 2:379003/151‑1 (MQ=255) atgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTccgc < 1:815295/151‑1 (MQ=255) gaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTccgccc > 2:960854/1‑151 (MQ=255) aCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTccgcccg < 1:891622/151‑1 (MQ=255) tGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCcgcccgcc > 2:354460/1‑151 (MQ=255) gAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCcgcccgcc > 2:399187/1‑150 (MQ=255) tCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGt > 2:435503/1‑150 (MQ=255) agaagaATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCgg > 2:1158066/1‑150 (MQ=255) atttcccccGCTTATCCCCCCCTCCCCGAAATCGGGTAATCCGCTGGAAGATCCTAACCGCA‑AAC‑CCCCAGCAAAAGGAGCGGCTTGCCGAAGATCACCCCCGCGGC‑CGCCACA‑AAATTTCCGCTCCCCCCCGCCAGTAAACCGGACTTca < 1:403594/146‑1 (MQ=255) ttCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCacac > 2:675598/1‑151 (MQ=255) ccccGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCt > 1:46283/1‑151 (MQ=255) ccctttttgtccccccTCCCATAAACCAGGTCATCCGCTCAGAATTACTTACCGCC‑AAC‑CCCCGGAAAAAGGAGGGCCTTGCCGACGACCACCACCGCGGG‑CGCCACC‑AGAACACCAATCCCGCCCGCCAGTAAACCGGACTTCACACCTa < 1:859089/137‑1 (MQ=255) cccGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCt > 1:32337/1‑150 (MQ=255) ccttttttcccccccTCCCCAAAATGGGGTCATACTCTTCGAAATTCTTACCCCC‑AAA‑CCCCAGCAAAATGTGGGGCGTGCCGAGGCTCAACACCGCGAG‑CCCAACA‑AGATTCCCGTTCCGGCCCGCCAGTAAACCGGACTTCACAcc < 1:1131410/135‑1 (MQ=255) cTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTaaca > 2:929473/1‑150 (MQ=255) ttatcccccccTCCCCAAAATCGGTCCATACGCTTCGAGATACTAACCGCC‑AAA‑CCCAAGAAAAAGGGGCGGCTGCCCGACGTCCAACCCCGCGAG‑CCCCCCA‑AAAATACCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTaacaa < 1:860702/141‑1 (MQ=255) aTTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAAcc > 1:850729/1‑150 (MQ=255) tcccccccTTCCCAAAACCAGCTCATCCGCTTGGAAAT‑TTTAACCCCAAAA‑CCCAAGCAAAAGGGGCGGCTTGCCAACTACAAACCCCGCGCG‑C‑CCACCAAAAATCCCGTTCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCa < 1:806914/144‑1 (MQ=255) cgcccctcccaaaaaTCAGGTAATCCGCTTCGAGATACTAACCGCA‑AAA‑CCCCAGAAAAAGGGGCGGCTTGCCGACAACCACCCCCGCGCG‑CGCCCCA‑AAAATCCCGATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACgg < 1:240697/139‑1 (MQ=255) cgcccctcccaaaaaCCAGGTAATACCCTTCGAAATCCTAACCCCA‑AAA‑CCCAAGCAAAAGGGGCGGCTGGCCGACGACAACCCCCGCGAG‑CCCCCCA‑AAAAACCCGTTCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACg < 1:77137/138‑1 (MQ=255) cACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTc > 1:721293/1‑151 (MQ=255) aCCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCt > 1:981552/1‑151 (MQ=255) ccccccaaaaaCCAGGTCATCCGCTTAGAAATACTAAACACC‑AAC‑CCCCGGGGAAATGGGCGGCTCACCGCCAATCACCCCCGCGAG‑CCCCCCA‑AAAACACCGATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCtg < 2:211897/143‑1 (MQ=255) ccTCCCCAAAAACAGGTAATGCTCTTAGAGATACTAAACCCA‑AAA‑CCCCAGCGAAATGAGCGGCTTCCCAACGACCACCCCCGCGGG‑CCCCACC‑AAAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCt < 1:315177/150‑1 (MQ=255) cTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGCAAACCGGACTTCACACCTAACAACCACGGTCtgg > 1:1093783/1‑151 (MQ=255) ataTCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGCATACCGATTCCGCCCGCCAGTCAACCGGCCTTCACACCTAACAACCACGGTCTGGTGGTtcg > 2:504894/3‑151 (MQ=255) tagtccccccGCTTCGACACAATCAACACA‑AAA‑CACCAGAAAATGGGGCGGCTTCCCGAC‑AACTACCCCGCGCGCCCCCACC‑AAATCCCCGCGCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCgg < 1:808500/141‑1 (MQ=255) aGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGAt > 2:603564/1‑151 (MQ=255) accccctgggaaaTACTAAACGCA‑AAC‑CCCCAGAGAAGGGAGCGGTTGCCCGGAAAACAACCCCGCGCG‑CCCCACA‑AGAACACCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGa < 1:328864/133‑1 (MQ=255) tACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAAc > 1:538175/1‑151 (MQ=255) ttCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATc > 2:129728/1‑151 (MQ=255) ggagaTATTCAACGAC‑AAC‑CACCAATAAAGTGGGCGGCTTCCCCACGCCCAACCCGGCGAG‑CCCACCC‑AGAAGGCCTTTTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCAc < 2:1069544/150‑1 (MQ=255) cGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCAc > 2:555240/1‑151 (MQ=255) gagaTACTTAACGCC‑AAA‑CAGCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGCTGCGCGGATCTAAACGCATCAcc > 2:26445/1‑151 (MQ=255) gagttactAACCCC‑AAACCCCCAGCAAAAGGGGCGGCTTGCCGCCCACCACCCCCGCGGG‑CCCCCCA‑AAAACCCTCATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCAc < 1:675807/143‑1 (MQ=255) agaTACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACct > 1:744532/1‑151 (MQ=255) aTACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCACCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACctct > 2:1059040/1‑151 (MQ=255) tACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTg > 2:894326/1‑151 (MQ=255) actaaccgac‑AAA‑CACCAGCAAAACGAGCGGCTTCCCGACAATCAACCCCGCGCG‑CCCCACC‑AAAATACCGTTCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACctct < 1:355086/140‑1 (MQ=255) ttAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGt > 2:16418/1‑151 (MQ=255) accGCC‑AAA‑CCCCAGAGAAATGGGCGGCTTGCCGACGATCACCCCCGCGAG‑CGCCACC‑AAAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGt < 1:283159/147‑1 (MQ=255) cc‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAAc > 1:996452/1‑151 (MQ=255) aaa‑CCCCAGAAAAAGGGGCGGCTTCCCGACGACCACCCCCGCGAG‑CCCCACA‑AAAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAAc < 1:156471/149‑1 (MQ=255) gggcgcccccccaacgaTCACCCCCGCGAG‑CCCCACA‑AAAACCCCGTCCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAAcc < 2:168459/137‑1 (MQ=255) ggCTTGCCGACAATCAACCCCGCGCG‑CCCCACC‑AGACTACCGATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTg < 1:1156653/151‑1 (MQ=255) tcgCCGACAAACAACCCCGCGAG‑CCCCCCC‑AAAACCCCGTTCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTg < 1:444141/146‑1 (MQ=255) acgaTCAACCCCGCGAC‑CCCCCCC‑AGAAAACCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCt < 2:939087/141‑1 (MQ=255) acgaTCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcgg > 1:216201/1‑151 (MQ=255) caaccacccccGCGAG‑CCCCACA‑AGAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcggc < 1:435518/142‑1 (MQ=255) gaTCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcggcg > 1:520438/1‑151 (MQ=255) ccGCGAG‑CCCCACA‑AGAATCCCCACCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTc < 2:197289/148‑1 (MQ=255) ccGCGAC‑CGCCACA‑AAAATCCCGCCCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcggcg < 2:983444/143‑1 (MQ=255) gAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAAt > 2:599634/1‑151 (MQ=255) gacccccacc‑aAAACCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCgaag < 2:865187/143‑1 (MQ=255) aaaaccccGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGc < 1:504592/143‑1 (MQ=255) aGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCg > 1:14384/1‑150 (MQ=255) | GAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCC‑AAA‑CACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAG‑CGCCACC‑AGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCG > NC_000913/3131291‑3131578 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |