Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,131,4400TC41.4% 94.9 / 14.5 58G323G (GGA→GGGyghQputative inner membrane polysaccharide flippase
Reads supporting (aligned to +/- strand):  ref base T (29/5);  new base C (0/24);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.85e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCG  >  NC_000913/3131291‑3131578
                                                                                                                                                         |                                                                                                                                          
gaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTc                                                                                                                                           >  1:514789/1‑151 (MQ=255)
gaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTc                                                                                                                                           <  2:302165/151‑1 (MQ=255)
 aaatcaCTGAGTCAGCCGAGAAGAATTTTCCCGCTTATTCGCTCCTTCCCTAAATCAGCTCATACGCTTCGAGATACTTACCGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTcc                                                                                                                                          <  2:379003/151‑1 (MQ=255)
   atgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccgc                                                                                                                                        <  1:815295/151‑1 (MQ=255)
     gaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccgccc                                                                                                                                      >  2:960854/1‑151 (MQ=255)
      aCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccgcccg                                                                                                                                     <  1:891622/151‑1 (MQ=255)
        tGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCcgcccgcc                                                                                                                                   >  2:354460/1‑151 (MQ=255)
         gAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCcgcccgcc                                                                                                                                   >  2:399187/1‑150 (MQ=255)
            tCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGt                                                                                                                                >  2:435503/1‑150 (MQ=255)
                   agaagaATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCgg                                                                                                                         >  2:1158066/1‑150 (MQ=255)
                        atttcccccGCTTATCCCCCCCTCCCCGAAATCGGGTAATCCGCTGGAAGATCCTAACCGCAAACCCCCAGCAAAAGGAGCGGCTTGCCGAAGATCACCCCCGCGGCCGCCACAAAATTTCCGCTCCCCCCCGCCAGTAAACCGGACTTca                                                                                                                   <  1:403594/146‑1 (MQ=255)
                           ttCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCacac                                                                                                                >  2:675598/1‑151 (MQ=255)
                             ccccGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCt                                                                                                              >  1:46283/1‑151 (MQ=255)
                              ccctttttgtccccccTCCCATAAACCAGGTCATCCGCTCAGAATTACTTACCGCCAACCCCCGGAAAAAGGAGGGCCTTGCCGACGACCACCACCGCGGGCGCCACCAGAACACCAATCCCGCCCGCCAGTAAACCGGACTTCACACCTa                                                                                                             <  1:859089/137‑1 (MQ=255)
                              cccGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCt                                                                                                              >  1:32337/1‑150 (MQ=255)
                               ccttttttcccccccTCCCCAAAATGGGGTCATACTCTTCGAAATTCTTACCCCCAAACCCCAGCAAAATGTGGGGCGTGCCGAGGCTCAACACCGCGAGCCCAACAAGATTCCCGTTCCGGCCCGCCAGTAAACCGGACTTCACAcc                                                                                                               <  1:1131410/135‑1 (MQ=255)
                                  cTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTaaca                                                                                                          >  2:929473/1‑150 (MQ=255)
                                   ttatcccccccTCCCCAAAATCGGTCCATACGCTTCGAGATACTAACCGCCAAACCCAAGAAAAAGGGGCGGCTGCCCGACGTCCAACCCCGCGAGCCCCCCAAAAATACCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTaacaa                                                                                                         <  1:860702/141‑1 (MQ=255)
                                     aTTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAAcc                                                                                                       >  1:850729/1‑150 (MQ=255)
                                      tcccccccTTCCCAAAACCAGCTCATCCGCTTGGAAATTTTAACCCCAAAACCCAAGCAAAAGGGGCGGCTTGCCAACTACAAACCCCGCGCGCCCACCAAAAATCCCGTTCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCa                                                                                                      <  1:806914/144‑1 (MQ=255)
                                        cgcccctcccaaaaaTCAGGTAATCCGCTTCGAGATACTAACCGCAAAACCCCAGAAAAAGGGGCGGCTTGCCGACAACCACCCCCGCGCGCGCCCCAAAAATCCCGATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACgg                                                                                                   <  1:240697/139‑1 (MQ=255)
                                        cgcccctcccaaaaaCCAGGTAATACCCTTCGAAATCCTAACCCCAAAACCCAAGCAAAAGGGGCGGCTGGCCGACGACAACCCCCGCGAGCCCCCCAAAAAACCCGTTCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACg                                                                                                    <  1:77137/138‑1 (MQ=255)
                                          cACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTc                                                                                                 >  1:721293/1‑151 (MQ=255)
                                           aCCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCt                                                                                                >  1:981552/1‑151 (MQ=255)
                                            ccccccaaaaaCCAGGTCATCCGCTTAGAAATACTAAACACCAACCCCCGGGGAAATGGGCGGCTCACCGCCAATCACCCCCGCGAGCCCCCCAAAAACACCGATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCtg                                                                                               <  2:211897/143‑1 (MQ=255)
                                            ccTCCCCAAAAACAGGTAATGCTCTTAGAGATACTAAACCCAAAACCCCAGCGAAATGAGCGGCTTCCCAACGACCACCCCCGCGGGCCCCACCAAAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCt                                                                                                <  1:315177/150‑1 (MQ=255)
                                             cTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGCAAACCGGACTTCACACCTAACAACCACGGTCtgg                                                                                              >  1:1093783/1‑151 (MQ=255)
                                                    ataTCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGCATACCGATTCCGCCCGCCAGTCAACCGGCCTTCACACCTAACAACCACGGTCTGGTGGTtcg                                                                                       >  2:504894/3‑151 (MQ=255)
                                                        tagtccccccGCTTCGACACAATCAACACAAAACACCAGAAAATGGGGCGGCTTCCCGACAACTACCCCGCGCGCCCCCACCAAATCCCCGCGCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCgg                                                                                    <  1:808500/141‑1 (MQ=255)
                                                         aGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGAt                                                                                  >  2:603564/1‑151 (MQ=255)
                                                              accccctgggaaaTACTAAACGCAAACCCCCAGAGAAGGGAGCGGTTGCCCGGAAAACAACCCCGCGCGCCCCACAAGAACACCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGa                                                                                   <  1:328864/133‑1 (MQ=255)
                                                               tACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAAc                                                                            >  1:538175/1‑151 (MQ=255)
                                                                    ttCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATc                                                                       >  2:129728/1‑151 (MQ=255)
                                                                      ggagaTATTCAACGACAACCACCAATAAAGTGGGCGGCTTCCCCACGCCCAACCCGGCGAGCCCACCCAGAAGGCCTTTTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCAc                                                                     <  2:1069544/150‑1 (MQ=255)
                                                                      cGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCAc                                                                     >  2:555240/1‑151 (MQ=255)
                                                                       gagaTACTTAACGCCAAACAGCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGCTGCGCGGATCTAAACGCATCAcc                                                                    >  2:26445/1‑151 (MQ=255)
                                                                        gagttactAACCCCAAACCCCCAGCAAAAGGGGCGGCTTGCCGCCCACCACCCCCGCGGGCCCCCCAAAAACCCTCATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCAc                                                                     <  1:675807/143‑1 (MQ=255)
                                                                        agaTACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACct                                                                   >  1:744532/1‑151 (MQ=255)
                                                                          aTACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCACCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACctct                                                                 >  2:1059040/1‑151 (MQ=255)
                                                                           tACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTg                                                                >  2:894326/1‑151 (MQ=255)
                                                                            actaaccgacAAACACCAGCAAAACGAGCGGCTTCCCGACAATCAACCCCGCGCGCCCCACCAAAATACCGTTCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACctct                                                                 <  1:355086/140‑1 (MQ=255)
                                                                              ttAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGt                                                             >  2:16418/1‑151 (MQ=255)
                                                                                accGCCAAACCCCAGAGAAATGGGCGGCTTGCCGACGATCACCCCCGCGAGCGCCACCAAAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGt                                                             <  1:283159/147‑1 (MQ=255)
                                                                                    ccAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAAc                                                       >  1:996452/1‑151 (MQ=255)
                                                                                       aaaCCCCAGAAAAAGGGGCGGCTTCCCGACGACCACCCCCGCGAGCCCCACAAAAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAAc                                                       <  1:156471/149‑1 (MQ=255)
                                                                                                       gggcgcccccccaacgaTCACCCCCGCGAGCCCCACAAAAACCCCGTCCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAAcc                                       <  2:168459/137‑1 (MQ=255)
                                                                                                           ggCTTGCCGACAATCAACCCCGCGCGCCCCACCAGACTACCGATCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTg                                  <  1:1156653/151‑1 (MQ=255)
                                                                                                              tcgCCGACAAACAACCCCGCGAGCCCCCCCAAAACCCCGTTCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTg                                  <  1:444141/146‑1 (MQ=255)
                                                                                                                    acgaTCAACCCCGCGACCCCCCCCAGAAAACCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCt                                   <  2:939087/141‑1 (MQ=255)
                                                                                                                    acgaTCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcgg                         >  1:216201/1‑151 (MQ=255)
                                                                                                                     caaccacccccGCGAGCCCCACAAGAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcggc                        <  1:435518/142‑1 (MQ=255)
                                                                                                                      gaTCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcggcg                       >  1:520438/1‑151 (MQ=255)
                                                                                                                              ccGCGAGCCCCACAAGAATCCCCACCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTc                  <  2:197289/148‑1 (MQ=255)
                                                                                                                              ccGCGACCGCCACAAAAATCCCGCCCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcggcg                       <  2:983444/143‑1 (MQ=255)
                                                                                                                                  gAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAAt           >  2:599634/1‑151 (MQ=255)
                                                                                                                                   gacccccaccaAAACCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCgaag              <  2:865187/143‑1 (MQ=255)
                                                                                                                                              aaaaccccGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGc   <  1:504592/143‑1 (MQ=255)
                                                                                                                                              aGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCg  >  1:14384/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                          
GAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCG  >  NC_000913/3131291‑3131578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: