Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913213,3410AG42.3% 67.4 / 18.2 52G337G (GGA→GGG) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (5/25);  new base G (22/0);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 3.07e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC  >  NC_000913/213195‑213490
                                                                                                                                                  |                                                                                                                                                     
gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgtg                                                                                                                                                   <  1:19843/151‑1 (MQ=255)
     aCCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAggg                                                                                                                                                   >  2:165579/1‑144 (MQ=255)
       ccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACa                                                                                                                                               >  2:881920/1‑148 (MQ=255)
              aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAggggaccacctaa                                                                                                                                         >  1:404987/1‑135 (MQ=255)
              aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGc                                                                                                                                      <  2:899815/150‑1 (MQ=255)
              aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGc                                                                                                                                      <  1:641441/150‑1 (MQ=255)
              aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCg                                                                                                                                     <  2:803281/151‑1 (MQ=255)
                  ttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGa                                                                                                                                  <  2:928399/150‑1 (MQ=255)
                       cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCCTAAAGCGGGAgggg                                                                                                                              >  2:705763/1‑147 (MQ=255)
                            ttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGTAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTc                                                                                                                        <  1:577764/150‑1 (MQ=255)
                            ttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTc                                                                                                                        <  2:27679/150‑1 (MQ=255)
                             tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGAGAGTACAGCTTAAAGCGGGGGGCGGTAtttg                                                                                                                       >  1:941651/1‑148 (MQ=255)
                              gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGcc                                                                                                                     <  2:241083/151‑1 (MQ=255)
                                    cGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcgc                                                                                                               <  2:591191/151‑1 (MQ=255)
                                     gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGAAGGGGAAAGCTTAAAGCGGGGGGGGATATTCAccccctccg                                                                                                              >  2:403306/1‑145 (MQ=255)
                                       cGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGGGGCGATATTCGCCCTcccc                                                                                                               >  1:228297/1‑146 (MQ=255)
                                           aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCa                                                                                                          <  2:379244/149‑1 (MQ=255)
                                             cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGAc                                                                                                       <  2:484303/150‑1 (MQ=255)
                                                            tggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGAAAACTTTATGCGGGGGGCGGGATTTGCCCCCCGCGGGCCGACGAAGAGGTCAgcgtg                                                                                        >  2:388020/1‑150 (MQ=255)
                                                                  aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTc                                                                                  <  1:39928/150‑1 (MQ=255)
                                                                  aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCa                                                                                 <  1:318055/151‑1 (MQ=255)
                                                                        gTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGGGGAAAAGTTAATGCGGGAGGCGATATTTGCCCCCCGCGCGCAGACGAAGCGGGCAgcgggggggttaaagcg                                                                            >  1:524087/1‑136 (MQ=255)
                                                                        gTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGTGTTTCAAAgcgc                                                                           <  1:877324/151‑1 (MQ=255)
                                                                          cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGAAAGCTTAATGCGGGGGGGGATATTCGCCCCCCCCGTGCAGACGAAGCGGTCCGCGTGCGTTTCAAAACGcc                                                                          >  1:930986/1‑150 (MQ=255)
                                                                                gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtg                                                                   <  1:15732/151‑1 (MQ=255)
                                                                                gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGTACAGCTTAATGCGGGAGGGGGTAATCGCCCCCCGCGTGCAGACGAAGCGGGCAGGGTGCGGTTCAAAGCGCCCGGGtt                                                                    >  2:845621/1‑150 (MQ=255)
                                                                                 cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGGGGGGGGGGGTATTTGCCCTCCCCGTGGAGACGAAGCGGGCAGCGGGCGTTTTAAAACGCCCgg                                                                       >  1:619188/1‑146 (MQ=255)
                                                                                 cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAAAGCGGGGGGGGATATTTGCCCCCCCCGTGCAGAAGAAGCGGGCAGCGGGCGTTTCAAAACGCCCGGGtt                                                                    >  2:805129/1‑149 (MQ=255)
                                                                                   aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGTCACCTTAATGCGGGGGGCGGTATTTGCCCTCCCCGGGGCGACGAAGCGGGCAGCGTGCGTTTCCAAAGGGCAGGATttct                                                                 >  2:567664/1‑150 (MQ=255)
                                                                                   aaGCGAAAACATTGTGCCGTGGCAGACGTGACTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgctg                                                                <  1:857452/151‑1 (MQ=255)
                                                                                    aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGAGGGGGTATTCGCCCTCCGCGTGCAGACGAAGCGGGCAGCGTGCGGTTTAAAACGCCAGGATtg                                                                   >  2:679042/1‑147 (MQ=255)
                                                                                     gCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTTGGGAGAGTACAACTTAAAGCGGGGGGCGATATTTGCCCTCCGCGGGGAGACGAAGCGGGGAGAGGGGGGTTCAAAGCGCCAgg                                                                       >  2:365024/1‑142 (MQ=255)
                                                                                      cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAgc                                                                             <  1:537091/135‑1 (MQ=255)
                                                                                      cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCat                                                             <  2:344225/151‑1 (MQ=255)
                                                                                            cATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTAAAACTTAAAGCGGGGGGCGGTATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGTGCGGTTTAAAGCGCCCGGATTGCTGCATAtttt                                                        >  1:831337/1‑148 (MQ=255)
                                                                                            cATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGCGATATTTTCCCTCCGCGCGCAGACGAAGCGGGCAGCGGGGGGTTCAAAACGCCCGGAGTGCTGCATAtttt                                                        >  2:226898/1‑148 (MQ=255)
                                                                                             aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCg                                                      <  2:491866/151‑1 (MQ=255)
                                                                                             aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCg                                                      <  2:529637/151‑1 (MQ=255)
                                                                                                gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGGCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCggg                                                    >  2:105615/1‑150 (MQ=255)
                                                                                                    cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGCGATATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGTGCGTTTCAAAACGCCAGGATTTCTGCATATTTTCggggg                                                  >  2:414939/1‑146 (MQ=255)
                                                                                                        gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                           <  1:881903/151‑1 (MQ=255)
                                                                                                         cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggcg                                          <  1:588894/151‑1 (MQ=255)
                                                                                                          aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAAAGCGGGGGGGGGTATTTGCCCCCCCCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAACGCCCGGGTTGCTGCCTATTTTCGGGGGTAAcggcgg                                         >  1:939667/1‑151 (MQ=255)
                                                                                                               tGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAAAGCGGGAGGGGATATTTGCCCTCCGCGGGCAGACGAAGCGGGCAGCGGGCGTTTTAAAGCGCCAGGGTTGCTGCATATTTTCGGGCGGAAcggcgggc                                       >  2:181022/1‑146 (MQ=255)
                                                                                                                      aaCCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACCGCTTTATTCGGGAGGCGCTATTTGCCCCCCGCGGGCAGAAGGAGTGGGGCGCGTGCGGTTTAAAAAGCCCGGGGTGTTTCATAGTTTCTGGGGGAACGGCGGGGGTAAGATaa                              >  1:292934/1‑150 (MQ=255)
                                                                                                                       aCCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGGGGTATTCGCCCTCCGCGTGCAGACGAAGCGGGCCGCGGGGGGTTTAAAACGCCAGGATTGCTTCATATTGTCGGGCGGAACGGCGGGAGGAAGCTaa                              >  2:935004/1‑149 (MQ=255)
                                                                                                                         cGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGaa                          <  1:288816/151‑1 (MQ=255)
                                                                                                                           cTGGAATTACCGGCGGGGCTGGGGAGGGGAAAGCCTAATGCGGGGGGCGATATTTGCCCTCCGCGTGCCGACGAAGCGGGCAGCGTGCGGTTCAAAACGCCAGGGTTGCTGCATATTGTCGGGGGGAAAGGCGGGCGGAAGGTTaaaaaa                         >  1:214550/1‑150 (MQ=255)
                                                                                                                                   aCCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCaa                <  1:171583/151‑1 (MQ=255)
                                                                                                                                     cggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAaga              <  2:265666/151‑1 (MQ=255)
                                                                                                                                          gggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg         >  2:938536/1‑151 (MQ=255)
                                                                                                                                                 gAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgcc  >  2:27894/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC  >  NC_000913/213195‑213490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑