Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,1090AC44.6% 95.1 / 16.3 57G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base A (28/3);  new base C (0/25);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.18e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAT  >  NC_000913/4537960‑4538255
                                                                                                                                                       |                                                                                                                                                  
aTCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCAc                                                                                                                                                   >  2:317616/1‑151 (MQ=255)
  ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCAcc                                                                                                                                                  <  1:923108/150‑1 (MQ=255)
    ccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCttt                                                                                                                                               >  1:868568/1‑151 (MQ=255)
             cGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAAc                                                                                                                                      <  2:324893/151‑1 (MQ=255)
                 aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAAccgcc                                                                                                                                  >  1:469937/1‑151 (MQ=255)
                    tACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACcgccgc                                                                                                                                >  1:436219/1‑150 (MQ=255)
                       gCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCAcc                                                                                                                             >  2:850403/1‑150 (MQ=255)
                                      gaCGGGAGCAAGCTTATTCCATTTTAAATTATTCCCGGTAAAATCAATTTCAAATACGGCACCCGTCCGCAATCCTGTTTTGGCTTCGCCATTAATAAGCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCa                                                                                                              <  2:403161/150‑1 (MQ=255)
                                      gaCGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTAcc                                                                                                               >  1:277904/1‑149 (MQ=255)
                                               aaGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCg                                                                                                    >  2:962197/1‑151 (MQ=255)
                                                   ttATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                  >  2:210278/1‑149 (MQ=255)
                                                        ccATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa                                                                                            >  2:380409/1‑150 (MQ=255)
                                                               aatttattcCCGGTAAAATCAATTTAAAATCCGGCACCCGTTCGAATCCCTGTTTTGGTTTCCCCATTAATAAGCCAGTTTTTATCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCa                                                                                      <  2:830050/146‑1 (MQ=255)
                                                               aaaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                    >  1:233832/1‑151 (MQ=255)
                                                                aaTTATTCCCGGTAAAATAAATTTAAAATACGCCATCCGTTCCAAACCCGGTTTGGGCTCCGCAATTAAAAAGCCAGTTTTTATCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                   <  2:634387/151‑1 (MQ=255)
                                                                    attaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                               >  2:901099/1‑151 (MQ=255)
                                                                     ttcccGGTAAAATCAAGTTCAAATCCGGCATCCGTTCGCAACCCTGTTTGGGCTTCCCCATTAAAAAGCCAGGTTTTACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                               <  2:441449/147‑1 (MQ=255)
                                                                     ttaCGGGTGAAATCAAGTACAAATACGGCATCCGTCCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGTTTTTATCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                               <  2:41672/150‑1 (MQ=255)
                                                                      taCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                             >  2:682280/1‑151 (MQ=255)
                                                                      taCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                             >  2:826676/1‑151 (MQ=255)
                                                                        cgggTAAAATCAATTTAAAATACGGCATCCGTTCGCAATCCTGTTTGGGCTTCGCCATAAATAAGCCAGTTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                           <  2:485237/149‑1 (MQ=255)
                                                                        ccGGTAAAATAAATTTAAAATCCGGCATCCGTTCGAAATCCTGGTTTGGTTTCCCAATTAATAAGCCAGTTTTTATCCCCTTTATTCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                            <  2:432405/150‑1 (MQ=255)
                                                                              aaaTCAAGTAAAAATCCGTCATCCTTTCGAAATCCTGGTTTGGCTCCGCCATTAAAAAGCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt                                                                     <  2:771258/151‑1 (MQ=255)
                                                                                aTCAAGTTAAAATACGGCATCCTTTCGCAATCCTGGTTTGGCTTCACAATTAATAACCCAGGTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCa                                                                   <  2:24989/151‑1 (MQ=255)
                                                                                   aaGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                  >  2:546083/1‑149 (MQ=255)
                                                                                   aaGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                  >  2:509669/1‑149 (MQ=255)
                                                                                         aaaTCCGGCTTCCGTTCCCAACCTTGTTTTGGCTTCGCAATAAATAAGCAAGTTTTTACCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaa                                                           <  2:868584/150‑1 (MQ=255)
                                                                                         aaaTACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAAAAAGCCAGGTTTTACCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                             <  2:348734/148‑1 (MQ=255)
                                                                                                gCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACt                                                   >  2:819656/1‑151 (MQ=255)
                                                                                                    cctttCGCAACCTTGGTTTGGTTTCGCTTTAAATAACCCAGGTTTTACCCCTTTAATTCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt                                               <  1:853044/148‑1 (MQ=255)
                                                                                                    ccGTTCGCAATCCTGTTTTGGCTTCGCCATTAATAACCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt                                               <  2:98113/151‑1 (MQ=255)
                                                                                                    ccGTTCGCAATCCTGGTTTGGTTTCGCCATTAAAAAGCCAGGTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAt                                                <  2:254680/150‑1 (MQ=255)
                                                                                                    ccGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt                                               >  1:299650/1‑151 (MQ=255)
                                                                                                     cGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                              >  1:894031/1‑151 (MQ=255)
                                                                                                      gTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                              >  2:931306/1‑150 (MQ=255)
                                                                                                       ttCGCAATCCTGTTTTGGCTTCGCCATTAATAAGCCGGTTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                              <  2:437013/149‑1 (MQ=255)
                                                                                                       ttCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                             >  2:791136/1‑150 (MQ=255)
                                                                                                               ccTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat                                    >  2:89523/1‑151 (MQ=255)
                                                                                                                cgtgTTTGGTTTCCCCCATTAATAGCCCGGTTTTTACCGCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                              <  1:206908/138‑1 (MQ=255)
                                                                                                                   gTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttc                                >  2:390832/1‑151 (MQ=255)
                                                                                                                    tttGGCTTCGCAATTAAGAAGCCGGTTTTTATCCCCTTTATCCAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat                                    <  1:112826/146‑1 (MQ=255)
                                                                                                                     ttGGCTCCCCCTTAAAAAAGCCAGTTTTTACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct                               <  2:889957/150‑1 (MQ=255)
                                                                                                                         gCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtt                           >  1:596500/1‑151 (MQ=255)
                                                                                                                            tCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttttt                        >  2:94419/1‑151 (MQ=255)
                                                                                                                             cGCAATTAAAAACCCAGTTTTAACCACTTTAATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttt                          <  1:967315/148‑1 (MQ=255)
                                                                                                                                ccATTAATAAGCCAGTTTTTATCCCCTTTACTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttt                          <  2:467114/146‑1 (MQ=255)
                                                                                                                                caaTTAATAAGCCAGGTTTTACCCCCTTTATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                         <  2:113195/145‑1 (MQ=255)
                                                                                                                                  aTTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                    >  1:716159/1‑150 (MQ=255)
                                                                                                                                     aataaGCCAGTTTTTATCCCCTTTTTTCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaaa                <  2:382129/151‑1 (MQ=255)
                                                                                                                                     aaaaacccAGGTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaa                  <  1:572956/143‑1 (MQ=255)
                                                                                                                                     aaaaaccagctttttATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCTAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCa                   <  1:913910/137‑1 (MQ=255)
                                                                                                                                     aaaaaGCCAGGTTTTACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa                 <  2:617463/147‑1 (MQ=255)
                                                                                                                                             aGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGc        >  1:360025/1‑151 (MQ=255)
                                                                                                                                              gtttttATCCCCTTTATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGc        <  1:74754/148‑1 (MQ=255)
                                                                                                                                                ttttACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa       <  2:510030/149‑1 (MQ=255)
                                                                                                                                                  ttATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg    >  2:866767/1‑150 (MQ=255)
                                                                                                                                                   tATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAt  >  2:743523/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
ATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAT  >  NC_000913/4537960‑4538255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: