Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,538,109 | 0 | A | C | 44.6% | 95.1 / 16.3 | 57 | G219G (GGT→GGG) | nanM | N‑acetylneuraminic acid mutarotase |
Reads supporting (aligned to +/- strand): ref base A (28/3); new base C (0/25); total (28/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.18e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAT > NC_000913/4537960‑4538255 | aTCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCAc > 2:317616/1‑151 (MQ=255) ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCAcc < 1:923108/150‑1 (MQ=255) ccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCttt > 1:868568/1‑151 (MQ=255) cGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAAc < 2:324893/151‑1 (MQ=255) aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAAccgcc > 1:469937/1‑151 (MQ=255) tACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACcgccgc > 1:436219/1‑150 (MQ=255) gCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCAcc > 2:850403/1‑150 (MQ=255) gaCGGGAGCAAGCTTATTCCATTTTAAATTATTCCCGGTAAAATCAATTTCAAATACGGCACCCGTCCGCAATCCTGTTTT‑GGCTTCG‑CCATTAATAAGCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCa < 2:403161/150‑1 (MQ=255) gaCGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTAcc > 1:277904/1‑149 (MQ=255) aaGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCg > 2:962197/1‑151 (MQ=255) ttATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc > 2:210278/1‑149 (MQ=255) ccATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa > 2:380409/1‑150 (MQ=255) aatttattcCCGGTAAAATCAATTTAAAATCCGGCACCCGTTCGAATCCCTGTTTT‑GGTTTCC‑CCATTAATAAGCCAGTTTTTATCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCa < 2:830050/146‑1 (MQ=255) aaaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt > 1:233832/1‑151 (MQ=255) aaTTATTCCCGGTAAAATAAATTTAAAATACGCCATCCGTTC‑CAAACCCGGTTTGGGCTCCG‑CAATTAAAAAGCCAGTTTTTATCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg < 2:634387/151‑1 (MQ=255) attaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt > 2:901099/1‑151 (MQ=255) ttcccGGTAAAATCAAGTTCAAATCCGGCATCCGTTCGCAACCCTGTTTG‑GGCTTCC‑CCATTAAAAAGCCAGGTTTTACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt < 2:441449/147‑1 (MQ=255) ttaCGGGTGAAATCAAGTACAAATACGGCATCCGTCCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGTTTTTATCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt < 2:41672/150‑1 (MQ=255) taCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt > 2:682280/1‑151 (MQ=255) taCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt > 2:826676/1‑151 (MQ=255) cgggTAAAATCAATTTAAAATACGGCATCCGTTCGCAATCCTGTTTG‑GGCTTCG‑CCATAAATAAGCCAGTTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg < 2:485237/149‑1 (MQ=255) ccGGTAAAATAAATTTAAAATCCGGCATCCGTTCGAAATCCTGGTTT‑GGTTTCC‑CAATTAATAAGCCAGTTTTTATCCCCTTTATTCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt < 2:432405/150‑1 (MQ=255) aaaTCAAGTAAAAATCCGTCATCCTTTCGAAATCCTGGTTT‑GGCTCCG‑CCATTAAAAAGCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt < 2:771258/151‑1 (MQ=255) aTCAAGTTAAAATACGGCATCCTTTCGCAATCCTGGTTT‑GGCTTCA‑CAATTAATAACCCAGGTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCa < 2:24989/151‑1 (MQ=255) aaGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa > 2:546083/1‑149 (MQ=255) aaGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa > 2:509669/1‑149 (MQ=255) aaaTCCGGCTTCCGTTCCCAACCTTGTTTT‑GGCTTCG‑CAATAAATAAGCAAGTTTTTACCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaa < 2:868584/150‑1 (MQ=255) aaaTACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAAAAAGCCAGGTTTTACCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac < 2:348734/148‑1 (MQ=255) gCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACt > 2:819656/1‑151 (MQ=255) cctttCGCAACCTTGGTTT‑GGTTTCG‑CTTTAAATAACCCAGGTTTTACCCCTTTAATTCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt < 1:853044/148‑1 (MQ=255) ccGTTCGCAATCCTGTTTT‑GGCTTCG‑CCATTAATAACCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt < 2:98113/151‑1 (MQ=255) ccGTTCGCAATCCTGGTTT‑GGTTTCG‑CCATTAAAAAGCCAGGTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAt < 2:254680/150‑1 (MQ=255) ccGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt > 1:299650/1‑151 (MQ=255) cGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg > 1:894031/1‑151 (MQ=255) gTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg > 2:931306/1‑150 (MQ=255) ttCGCAATCCTGTTTT‑GGCTTCG‑CCATTAATAAGCCGGTTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg < 2:437013/149‑1 (MQ=255) ttCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga > 2:791136/1‑150 (MQ=255) ccTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat > 2:89523/1‑151 (MQ=255) cgtgTTT‑GGTTTCCCCCATTAATAGCCCGGTTTTTACCGCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg < 1:206908/138‑1 (MQ=255) gTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttc > 2:390832/1‑151 (MQ=255) ttt‑GGCTTCG‑CAATTAAGAAGCCGGTTTTTATCCCCTTTATCCAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat < 1:112826/146‑1 (MQ=255) tt‑GGCTCCC‑CCTTAAAAAAGCCAGTTTTTACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct < 2:889957/150‑1 (MQ=255) gCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtt > 1:596500/1‑151 (MQ=255) tCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttttt > 2:94419/1‑151 (MQ=255) cG‑CAATTAAAAACCCAGTTTTAACCACTTTAATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttt < 1:967315/148‑1 (MQ=255) ccATTAATAAGCCAGTTTTTATCCCCTTTACTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttt < 2:467114/146‑1 (MQ=255) caaTTAATAAGCCAGGTTTTACCCCCTTTATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt < 2:113195/145‑1 (MQ=255) aTTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc > 1:716159/1‑150 (MQ=255) aataaGCCAGTTTTTATCCCCTTTTTTCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaaa < 2:382129/151‑1 (MQ=255) aaaaacccAGGTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaa < 1:572956/143‑1 (MQ=255) aaaaaccagctttttATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCTAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCa < 1:913910/137‑1 (MQ=255) aaaaaGCCAGGTTTTACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa < 2:617463/147‑1 (MQ=255) aGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGc > 1:360025/1‑151 (MQ=255) gtttttATCCCCTTTATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGc < 1:74754/148‑1 (MQ=255) ttttACCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa < 2:510030/149‑1 (MQ=255) ttATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg > 2:866767/1‑150 (MQ=255) tATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAt > 2:743523/1‑151 (MQ=255) | ATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTT‑GGCTTCG‑CCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAT > NC_000913/4537960‑4538255 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |