Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,590	C→T	G386D (GGT→GAT)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,590	0	C	T	100.0%	227.8 / NA	58	G386D (GGT→GAT)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/24);  total (34/24)

CGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCAT  >  NC_000913/2724443‑2724737                                                                                                                                                    |                                                                                                                                                    cGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAAccatca                                                                                                                                                   >  1:12065/1‑150 (MQ=255)   tCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAat                                                                                                                                                >  1:27536/1‑151 (MQ=255)     aCTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAatat                                                                                                                                              >  2:197250/1‑151 (MQ=255)     aCTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAatat                                                                                                                                              >  2:166697/1‑151 (MQ=255)       tAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAatat                                                                                                                                              >  2:325386/1‑149 (MQ=255)        aaaGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAAtata                                                                                                                                             <  2:113487/149‑1 (MQ=255)        aaaGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAg                                                                                                                                            <  2:780984/150‑1 (MQ=255)          aGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCAt                                                                                                                                         <  2:705588/151‑1 (MQ=255)            aCGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTa                                                                                                                                       <  1:703838/151‑1 (MQ=255)              gCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGc                                                                                                                                     >  2:854232/1‑151 (MQ=255)               cATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCg                                                                                                                                    <  1:11358/151‑1 (MQ=255)                aTCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCg                                                                                                                                    >  2:186700/1‑150 (MQ=255)                   cccttcccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCg                                                                                                                                <  1:379648/151‑1 (MQ=255)                       tcccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCat                                                                                                                             <  2:13359/150‑1 (MQ=255)                       tcccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCat                                                                                                                             >  1:646935/1‑150 (MQ=255)                                   gTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAAc                                                                                                                >  2:297784/1‑151 (MQ=255)                                     aGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGc                                                                                                              >  1:873150/1‑151 (MQ=255)                                       cATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCt                                                                                                            >  1:574710/1‑151 (MQ=255)                                          cAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAAt                                                                                                         >  1:840688/1‑151 (MQ=255)                                           aaaGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATg                                                                                                        >  2:526903/1‑151 (MQ=255)                                            aaGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgc                                                                                                       >  2:189983/1‑151 (MQ=255)                                                     gAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCtg                                                                                              <  1:710442/151‑1 (MQ=255)                                                      aaaCGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCtgt                                                                                             <  1:509813/151‑1 (MQ=255)                                                      aaaCGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCtgt                                                                                             >  1:9605/1‑151 (MQ=255)                                                          gCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCg                                                                                         >  1:890987/1‑151 (MQ=255)                                                           ccaccaCGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCgg                                                                                        <  2:417339/151‑1 (MQ=255)                                                                  ggCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAtc                                                                                 <  2:766609/151‑1 (MQ=255)                                                                  ggCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAtc                                                                                 <  1:796362/151‑1 (MQ=255)                                                                      aTCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAtctc                                                                               >  2:568501/1‑149 (MQ=255)                                                                         aaGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCt                                                                          <  2:142501/151‑1 (MQ=255)                                                                         aaGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCt                                                                          >  1:373795/1‑151 (MQ=255)                                                                          aGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCtt                                                                         <  2:680519/151‑1 (MQ=255)                                                                              cacaTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGt                                                                     >  2:534899/1‑151 (MQ=255)                                                                                 aTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTAtt                                                                  >  2:257364/1‑151 (MQ=255)                                                                                      agaagaAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACt                                                             <  1:72530/151‑1 (MQ=255)                                                                                      agaagaAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACt                                                             >  2:215029/1‑151 (MQ=255)                                                                                       gaagaaGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtg                                                            <  2:451155/151‑1 (MQ=255)                                                                                          gaagGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgatt                                                         >  1:963965/1‑151 (MQ=255)                                                                                            agGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgattg                                                        >  1:63796/1‑150 (MQ=255)                                                                                              gCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATg                                                     >  2:951430/1‑151 (MQ=255)                                                                                                tGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGt                                                    >  2:470372/1‑150 (MQ=255)                                                                                                      cATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaaaaa                                             <  2:27537/151‑1 (MQ=255)                                                                                                             aTTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACt                                       >  2:556343/1‑150 (MQ=255)                                                                                                             aTTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTa                                      <  1:157805/151‑1 (MQ=255)                                                                                                              ttGATTTCAGCGACAACGCTACGTACTCCGCCGCACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTAt                                     >  1:282442/1‑151 (MQ=255)                                                                                                               tGATTTCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATc                                    >  1:536521/1‑151 (MQ=255)                                                                                                                   ttCAGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagc                                 >  2:67507/1‑150 (MQ=255)                                                                                                                      aGCGACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAggg                             >  1:135282/1‑151 (MQ=255)                                                                                                                         gACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac                          >  2:279625/1‑151 (MQ=255)                                                                                                                         gACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac                          >  2:479453/1‑151 (MQ=255)                                                                                                                         gACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac                          >  1:656999/1‑151 (MQ=255)                                                                                                                         gACAACGCTACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac                          <  2:10284/151‑1 (MQ=255)                                                                                                                                 tACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCAcc                  >  1:893261/1‑151 (MQ=255)                                                                                                                                  aCGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCAcc                  >  1:918842/1‑150 (MQ=255)                                                                                                                                  aCGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCAcc                  <  1:429313/150‑1 (MQ=255)                                                                                                                                    gTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGa               <  1:491661/151‑1 (MQ=255)                                                                                                                                      aCTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAcc             <  2:12066/151‑1 (MQ=255)                                                                                                                                          cgccgAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCaaaa         <  1:186697/151‑1 (MQ=255)                                                                                                                                              gAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCgg     <  2:533618/151‑1 (MQ=255)                                                                                                                                                 ccatcaAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCAt  <  1:854216/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCAT  >  NC_000913/2724443‑2724737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑