Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,175	G→C	L185L (CTC→CTG)	pdxJ ←	pyridoxine 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,175	0	G	C	100.0%	215.0 / NA	53	L185L (CTC→CTG)	pdxJ	pyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (30/23);  total (30/23)

TTCTGCCACCGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCT  >  NC_000913/2701031‑2701316                                                                                                                                                 |                                                                                                                                              ttCTGCCACCGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCtt                                                                                                                                         >  1:248770/1‑151 (MQ=255)  tCTGCCACCGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTg                                                                                                                                        >  2:407604/1‑151 (MQ=255)    tGCCACCGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCg                                                                                                                                      >  1:853588/1‑151 (MQ=255)      ccACCGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGc                                                                                                                                    >  1:443857/1‑151 (MQ=255)         ccGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCaaa                                                                                                                                 <  1:592702/151‑1 (MQ=255)         ccGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCaaa                                                                                                                                 <  2:638619/151‑1 (MQ=255)          cGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAg                                                                                                                                <  1:710798/151‑1 (MQ=255)                   cAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCt                                                                                                                       <  1:447672/151‑1 (MQ=255)                    aGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCtt                                                                                                                      <  1:126744/151‑1 (MQ=255)                        cGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGc                                                                                                                  <  2:519437/151‑1 (MQ=255)                        cGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGc                                                                                                                  <  2:861897/151‑1 (MQ=255)                               caCTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATAcgc                                                                                                           >  2:482470/1‑151 (MQ=255)                                aCTGCACGACCAATAATGGCATGAACGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCCGGTTTGCGGCAAAGGTCGCGGCTTTGGCGATAcgcg                                                                                                          >  2:207956/1‑151 (MQ=255)                                         ccAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCtt                                                                                                 <  1:859874/151‑1 (MQ=255)                                           aataatGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTgc                                                                                               <  1:725152/151‑1 (MQ=255)                                            ataatGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTgcg                                                                                              <  1:268523/151‑1 (MQ=255)                                             taatGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTgcgc                                                                                             >  2:279787/1‑151 (MQ=255)                                                 ggCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGt                                                                                         <  2:99470/151‑1 (MQ=255)                                                  gCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCCGtt                                                                                        <  2:803344/151‑1 (MQ=255)                                                      gACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGc                                                                                    >  1:180809/1‑151 (MQ=255)                                                       aCCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCg                                                                                   >  2:259704/1‑151 (MQ=255)                                                         cGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTc                                                                                 >  1:138622/1‑151 (MQ=255)                                                          gATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTc                                                                                 >  1:139969/1‑150 (MQ=255)                                                           atatTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTc                                                                                 >  2:101466/1‑149 (MQ=255)                                                                  gTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGc                                                                        >  2:155897/1‑151 (MQ=255)                                                                          aTCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCAt                                                                >  2:325979/1‑151 (MQ=255)                                                                            ctcAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCAt                                                                >  1:119936/1‑149 (MQ=255)                                                                              cAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCAt                                                                <  1:692859/147‑1 (MQ=255)                                                                              cAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAg                                                              <  2:443866/149‑1 (MQ=255)                                                                              cAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGc                                                             >  2:906818/1‑150 (MQ=255)                                                                               aGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCa                                                            >  2:45425/1‑150 (MQ=255)                                                                                 ggATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAAcc                                                         <  2:802403/151‑1 (MQ=255)                                                                                    tGGCGACAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCgg                                                       >  1:821021/1‑150 (MQ=255)                                                                                     ggcggcAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGgtg                                                     <  1:412885/151‑1 (MQ=255)                                                                                      gcggcAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGgtgt                                                    <  2:365526/151‑1 (MQ=255)                                                                                        ggcAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTgg                                                  <  1:399772/151‑1 (MQ=255)                                                                                           aaTGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtc                                               <  1:471904/151‑1 (MQ=255)                                                                                            aTGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtct                                              >  1:444754/1‑151 (MQ=255)                                                                                              ggCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCg                                            >  2:929256/1‑151 (MQ=255)                                                                                              ggCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCg                                            >  1:644115/1‑151 (MQ=255)                                                                                               gCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCg                                            >  1:49913/1‑150 (MQ=255)                                                                                                  ttCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGAt                                          <  1:483228/149‑1 (MQ=255)                                                                                                     aCGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACg                                     >  2:832611/1‑151 (MQ=255)                                                                                                       gTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCTATAAAc                                      >  2:56387/1‑148 (MQ=255)                                                                                                                   cAGACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCACAGGCCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACct                         >  2:528324/1‑149 (MQ=255)                                                                                                                     gACCGTGTCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGc                     >  2:412753/1‑151 (MQ=255)                                                                                                                           gtCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAg                <  2:542860/150‑1 (MQ=255)                                                                                                                           gtCCGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGc               >  1:750164/1‑151 (MQ=255)                                                                                                                              cGGCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCttt            <  1:658345/151‑1 (MQ=255)                                                                                                                               ggCGTTAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTg           >  2:365426/1‑151 (MQ=255)                                                                                                                                    tAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGGTAGACGGTGCACCATCAACTACCGCAGCTTTGATCTg      >  1:941008/1‑151 (MQ=255)                                                                                                                                    tAACTTTCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCt       <  1:791609/150‑1 (MQ=255)                                                                                                                                        tttCAGACCCAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGctct  >  2:821822/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                              TTCTGCCACCGCATCTTTCAGTCCGGTCATCACTGCACGACCAATAATGGCATGACCGATATTCAGTTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCT  >  NC_000913/2701031‑2701316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑