Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,155	G→T	R198S (CGT→AGT)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,155	0	G	T	98.1%	207.3 / ‑6.2	53	R198S (CGT→AGT)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (28/24);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTC  >  NC_000913/2725009‑2725296                                                                                                                                                   |                                                                                                                                              gatgaATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCAt                                                                                                                                           <  2:21599/151‑1 (MQ=255)    gaATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATccc                                                                                                                                        >  1:870253/1‑151 (MQ=255)    gaATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATccc                                                                                                                                        <  1:967140/151‑1 (MQ=255)     aaTGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATcccc                                                                                                                                       <  1:663665/151‑1 (MQ=255)      aTGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATcccc                                                                                                                                       >  1:817077/1‑150 (MQ=255)         cACGGCGATTGCGCCACAATCCTTTCAGAGATCCAGCTTCTTTTAATGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCAGAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCActc                                                                                                                                   >  2:14772/1‑151 (MQ=255)          aCGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCActct                                                                                                                                  <  1:511832/151‑1 (MQ=255)                  tGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTg                                                                                                                           <  2:450754/150‑1 (MQ=255)                  tGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTg                                                                                                                           <  1:563038/150‑1 (MQ=255)                   gcgcCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGc                                                                                                                          <  1:152479/150‑1 (MQ=255)                             ccTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTcc                                                                                                                <  1:606184/150‑1 (MQ=255)                                ttCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCAt                                                                                                              <  2:183905/149‑1 (MQ=255)                                ttCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCAt                                                                                                              <  2:717039/149‑1 (MQ=255)                                  cAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGgtg                                                                                                          >  1:327693/1‑151 (MQ=255)                                      gaTCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACATAGCTCCTAACGCGAAAGGAATACTCAATCCCCACTCTCTGAGAGCAGCGTCTTCCATGGTGtgtt                                                                                                      >  1:77000/1‑151 (MQ=255)                                       aTCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGtgttg                                                                                                     >  2:925126/1‑151 (MQ=255)                                       aTCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGtgttg                                                                                                     <  1:111635/151‑1 (MQ=255)                                              ttcttTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAAcc                                                                                              >  1:446789/1‑151 (MQ=255)                                              ttcttTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAAcc                                                                                              >  1:780966/1‑151 (MQ=255)                                                  tttAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACg                                                                                           >  1:12358/1‑150 (MQ=255)                                                   ttAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGAc                                                                                         >  1:821306/1‑151 (MQ=255)                                                    tAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGAc                                                                                         >  2:499341/1‑150 (MQ=255)                                                     aaaGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACaa                                                                                       <  2:315158/151‑1 (MQ=255)                                                     aaaGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACa                                                                                        >  2:345786/1‑150 (MQ=255)                                                     aaaGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACaa                                                                                       <  1:620374/151‑1 (MQ=255)                                                        gcgcgcGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCa                                                                                    <  1:67779/151‑1 (MQ=255)                                                         cgcgcgTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAAcc                                                                                     >  1:961040/1‑149 (MQ=255)                                                                    tgttgCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAg                                                                          <  2:89419/149‑1 (MQ=255)                                                                      ttgCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGt                                                                      >  2:790539/1‑151 (MQ=255)                                                                        gCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTg                                                                    <  2:349883/151‑1 (MQ=255)                                                                               ttCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTccgcc                                                              <  2:179774/150‑1 (MQ=255)                                                                                tCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTccgccg                                                             <  1:530652/150‑1 (MQ=255)                                                                                 cATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGAt                                                           >  2:854934/1‑151 (MQ=255)                                                                                     tAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAAc                                                       >  2:793460/1‑151 (MQ=255)                                                                                             gacgTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACAt                                                >  1:440600/1‑150 (MQ=255)                                                                                                 tAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTg                                            >  2:865699/1‑150 (MQ=255)                                                                                                  aaCCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGa                                           >  1:521903/1‑150 (MQ=255)                                                                                                     cacaACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAatg                                       <  2:936064/151‑1 (MQ=255)                                                                                                     cacaACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAatg                                       >  2:329037/1‑151 (MQ=255)                                                                                                      acaACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAatga                                      <  1:485547/151‑1 (MQ=255)                                                                                                        aaCGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAatgatg                                    <  2:841763/151‑1 (MQ=255)                                                                                                        aaCGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAatgatg                                    >  1:4178/1‑151 (MQ=255)                                                                                                         aCGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGc                                   <  1:899022/151‑1 (MQ=255)                                                                                                            ccACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTa                                <  1:796012/151‑1 (MQ=255)                                                                                                                 acaGCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAAc                           <  1:731355/151‑1 (MQ=255)                                                                                                                    gCTAACACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCtt                        >  2:32626/1‑151 (MQ=255)                                                                                                                       aaCACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTgc                     >  2:365858/1‑151 (MQ=255)                                                                                                                       aaCACAGCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTgc                     >  1:50273/1‑151 (MQ=255)                                                                                                                             gCTCCTAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCtt               <  1:533462/151‑1 (MQ=255)                                                                                                                                  tAACGCGAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACg          >  1:32101/1‑151 (MQ=255)                                                                                                                                      gcgAAAGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCa       >  2:407508/1‑150 (MQ=255)                                                                                                                                          aaGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTc  >  1:977744/1‑151 (MQ=255)                                                                                                                                          aaGGAATACTCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTc  >  2:65072/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                              GATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTC  >  NC_000913/2725009‑2725296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑