Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,724,588 C→T G387S (GGT→AGT)  kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,724,5880CT98.4% 244.4 / ‑3.9 64G387S (GGT→AGT) kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (38/25);  total (39/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGC  >  NC_000913/2724443‑2724735
                                                                                                                                                 |                                                                                                                                                   
cGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCaa                                                                                                                                                <  1:265145/151‑1 (MQ=255)
 gTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATGTCAGCGACAACGCTACGTACTCCGCCGAACTACCaa                                                                                                                                                <  2:121740/150‑1 (MQ=255)
      tAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAatat                                                                                                                                            >  1:645174/1‑149 (MQ=255)
          gACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCAtt                                                                                                                                      <  1:260003/151‑1 (MQ=255)
          gACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCAtt                                                                                                                                      >  1:42322/1‑151 (MQ=255)
           aCGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTa                                                                                                                                     <  1:819513/151‑1 (MQ=255)
           aCGCATCCCCTTCCCTTTGCGATGTACCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCAtt                                                                                                                                      >  2:1008718/1‑150 (MQ=255)
            cGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTa                                                                                                                                     <  2:679037/150‑1 (MQ=255)
            cGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAg                                                                                                                                    <  1:312043/151‑1 (MQ=255)
              cATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCg                                                                                                                                  <  2:788723/151‑1 (MQ=255)
               aTCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCg                                                                                                                                  >  1:507187/1‑150 (MQ=255)
                   ccttcccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGc                                                                                                                             <  1:579281/151‑1 (MQ=255)
                      tcccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCat                                                                                                                           >  2:312307/1‑150 (MQ=255)
                       cccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCata                                                                                                                          <  2:964996/150‑1 (MQ=255)
                           ttGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGa                                                                                                                       <  1:752672/149‑1 (MQ=255)
                           ttGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGAc                                                                                                                      >  1:807260/1‑150 (MQ=255)
                               gATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGAcc                                                                                                                 >  2:137813/1‑151 (MQ=255)
                                aTGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCa                                                                                                                <  1:880365/151‑1 (MQ=255)
                                 tGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCa                                                                                                                >  1:744820/1‑150 (MQ=255)
                                 tGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCa                                                                                                                >  2:493971/1‑150 (MQ=255)
                                        tCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTa                                                                                                         >  1:549104/1‑150 (MQ=255)
                                           aaGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgc                                                                                                     >  2:532947/1‑151 (MQ=255)
                                             gAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgcgc                                                                                                   >  1:580219/1‑151 (MQ=255)
                                              aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGcgcg                                                                                                  >  2:288073/1‑151 (MQ=255)
                                                   aGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCt                                                                                             <  2:851848/151‑1 (MQ=255)
                                                   aGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCt                                                                                             >  1:373076/1‑151 (MQ=255)
                                                            accacGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCggg                                                                                     >  2:264684/1‑150 (MQ=255)
                                                               acGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAAc                                                                                  >  1:856274/1‑150 (MQ=255)
                                                                 ggCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAtc                                                                               >  1:131823/1‑151 (MQ=255)
                                                                  gCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCACGAACCTGTGCCGGGAACAt                                                                                >  2:431454/1‑149 (MQ=255)
                                                                   ccATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAtctc                                                                             >  1:510322/1‑151 (MQ=255)
                                                                     aTCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAtctc                                                                             >  2:859947/1‑149 (MQ=255)
                                                                     aTCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAg                                                                           <  1:965834/151‑1 (MQ=255)
                                                                             cacaTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGt                                                                   <  1:461717/151‑1 (MQ=255)
                                                                             cacaTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGt                                                                   <  1:8308/151‑1 (MQ=255)
                                                                             cacaTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGt                                                                   >  1:76996/1‑151 (MQ=255)
                                                                                    cAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCAc                                                            >  2:676761/1‑151 (MQ=255)
                                                                                       aagaagGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtg                                                          >  1:794646/1‑150 (MQ=255)
                                                                                               tGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGt                                                  >  1:762424/1‑150 (MQ=255)
                                                                                                gTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGtat                                                >  2:823549/1‑151 (MQ=255)
                                                                                                gTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGt                                                  <  2:828506/149‑1 (MQ=255)
                                                                                                gTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGt                                                  <  1:773373/149‑1 (MQ=255)
                                                                                                 tttCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGtat                                                >  1:330074/1‑150 (MQ=255)
                                                                                                   tCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaa                                              >  1:615740/1‑150 (MQ=255)
                                                                                                    ccATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaaa                                             <  2:770268/150‑1 (MQ=255)
                                                                                                        tCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACt                                         <  2:4619/150‑1 (MQ=255)
                                                                                                             ttcaTTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCAc                                      <  2:491311/145‑1 (MQ=255)
                                                                                                             ttGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTAt                                   >  2:323599/1‑151 (MQ=255)
                                                                                                              tGATTTCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTAt                                   <  1:877154/150‑1 (MQ=255)
                                                                                                                 tttCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagc                               >  1:921721/1‑151 (MQ=255)
                                                                                                                  ttCAGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagc                               >  2:719052/1‑150 (MQ=255)
                                                                                                                     aGCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagcag                             >  1:673212/1‑149 (MQ=255)
                                                                                                                      gCGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGc                          >  1:1061582/1‑151 (MQ=255)
                                                                                                                       cGACAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGc                          >  2:348000/1‑150 (MQ=255)
                                                                                                                          cAACGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcacac                      <  1:680968/151‑1 (MQ=255)
                                                                                                                            aCGCTACGTACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACAt                    <  1:493987/151‑1 (MQ=255)
                                                                                                                                    tACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAc            >  2:324937/1‑151 (MQ=255)
                                                                                                                                    tACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAc            >  1:496924/1‑151 (MQ=255)
                                                                                                                                    tACTCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAc            >  1:539379/1‑151 (MQ=255)
                                                                                                                                       tCCGCCGAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCa          >  2:748095/1‑150 (MQ=255)
                                                                                                                                          gccgAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCaaa        >  1:404597/1‑149 (MQ=255)
                                                                                                                                           ccgAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGc     <  1:70036/151‑1 (MQ=255)
                                                                                                                                            cgAACTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCg    >  1:750096/1‑151 (MQ=255)
                                                                                                                                              aaCTACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGc  <  1:56750/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGC  >  NC_000913/2724443‑2724735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑