Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,871	0	T	A	45.3%	67.6 / 29.4	53	G58G (GGT→GGA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (26/3);  new base A (0/24);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 4.17e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGT  >  NC_000913/1755721‑1756014                                                                                                                                                       |                                                                                                                                                cACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGt                                                                                                                                                 <  2:578315/151‑1 (MQ=255)    cATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaa                                                                                                                                               >  2:26499/1‑150 (MQ=255)       cGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAcc                                                                                                                                           <  2:59040/151‑1 (MQ=255)        ggACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccg                                                                                                                                          <  1:614183/151‑1 (MQ=255)         gACCCAAAACCGAATCTGGAGAACTGTTAGCTAAAATGCTGGACGCTGCCATGAACGTTATGCATGTGAACTTCTCTCACGGTGACTATGCAGAACACGGACAGCGCACTCAGAATCTGCGCAAAGCCATGAGCAAAACTGGTAAAAc                                                                                                                                            >  1:163616/1‑148 (MQ=255)            cGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACcgccgc                                                                                                                                      >  1:853688/1‑151 (MQ=255)               aaaCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTAt                                                                                                                                   >  2:502721/1‑151 (MQ=255)                        cggAAGAGATGTTATCAAAATGGCGGGCCGCGGGCATAACTGTTATGCTTCTAACCTTCTCGCAGGGTGACTATGAAGAACGCGGTCGCCCCATCCAAAATCGGCGCAACGTGAGAAGAAAAATTGGAAAAACCGCCGCTATCCTGCTTg                                                                                                                           <  2:799237/148‑1 (MQ=255)                          gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGAt                                                                                                                         >  1:147347/1‑150 (MQ=255)                           aaGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATAc                                                                                                                       >  2:117364/1‑151 (MQ=255)                            agagtttttacaaaaaaTGGTGGACGCTAGAAAGATCTTTATGCCTCTGACCTCCTCTAATAGTGACTATAAAAACCAGGGTCAGCCCATCCAGATTCGGCGCAAGGTGAGGAACAAAAGTGTAAAAACCGCCGCTATCCTGCTTGATAcc                                                                                                                      <  1:352254/138‑1 (MQ=255)                            agagATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATAcc                                                                                                                      >  1:107730/1‑151 (MQ=255)                                 ttttAGATAAAGTGGTGGACGCGGGAAGGACCGTTAGGTTTCTAAACTTCTCTCAGGGGGCATATGAAAAACAGGGGCAGCGCTTTCAAACTCTGCGAAGGGTGATGAGAAAAACTGAAAAACCCGCCGCTATCCTGCTTGATACCa                                                                                                                     <  1:469665/145‑1 (MQ=255)                                   ttAGATAAAAGGCGGGACGCTGGCAGAAACTTTATGCGTCTGAACTTCTCTCAGGGTAACTAGGCAAAACACGGCCAGCGAATTCAGAATCCGCGCAAGGTAATGAAAAAAACGGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGt                                                                                                                <  1:731221/150‑1 (MQ=255)                                         aaaaTGCTGGACGCGGGCATAACCGTTATGCTTCTGAACTTCTCTCAGGGTGACTATGCAGAACACGGTCAGCCCATACAGAATCGGCGCAAGGTAAAAAAAAAAACTAAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                                                                                                           <  1:564794/149‑1 (MQ=255)                                           aaTGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATc                                                                                                       >  1:487827/1‑151 (MQ=255)                                                gggACGCGGGCAGAAACTTTAGGCGTCTAAACTTCTCTCATGGTAACTAAGAAAAACACGGTAACCGAATGCAGACTCGGCACAACGTAAGAAAAAAAAGTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCAc                                                                                                  <  1:471760/150‑1 (MQ=255)                                                 ggccGCTGGCATGACCTTTAGGCGTCTAACTTTCTCTCATGGTGACTATGAAAAACCCGGCCAGCGCTTTAAAAATCTGCACAACGGAAGGAAAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCa                                                                                                   <  2:849734/146‑1 (MQ=255)                                                   aCGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCAt                                                                                               >  2:921384/1‑151 (MQ=255)                                                             gAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGa                                                                                      >  2:156939/1‑150 (MQ=255)                                                                  ttATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCgg                                                                                >  1:775468/1‑151 (MQ=255)                                                                    aTGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGt                                                                               >  2:424660/1‑150 (MQ=255)                                                                       cGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAacg                                                                           >  2:215785/1‑151 (MQ=255)                                                                            gAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGtt                                                                      >  1:879462/1‑151 (MQ=255)                                                                                   tctcATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTg                                                                >  2:376163/1‑150 (MQ=255)                                                                                   tctcATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTg                                                                >  2:991898/1‑150 (MQ=255)                                                                                      cATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGaa                                                              >  2:815654/1‑149 (MQ=255)                                                                                          ggaacaaTGCAGACCGCGGTCAGCGCATTAAAAATCTGCGCAAGGTGAGGAGAAAAAGGGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCt                                                          <  2:581435/143‑1 (MQ=255)                                                                                             aCTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTc                                                      >  2:183284/1‑150 (MQ=255)                                                                                             aCTATGCAAAACACGGCCACCGCTTTCACAATCTGCGAAACGTGAGAAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCt                                                          <  1:709639/146‑1 (MQ=255)                                                                                                aTGAAGAACACGTGCACCCAATTCAAAACCTGCGAAACGTAAGGAAAAAAAGGGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGa                                                   <  1:306933/150‑1 (MQ=255)                                                                                                   cAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCtt                                               >  2:845401/1‑151 (MQ=255)                                                                                                    aaaaCACGGCCAGCGTATACAGAATCGCCGCAAGGTGAGAAGAAAAGCTGAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGa                                                   <  1:635855/144‑1 (MQ=255)                                                                                                     gAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCttt                                              >  2:297229/1‑150 (MQ=255)                                                                                                      aaCGCGGTCAGCGCATTCAAAATCGGCACAACGTGATGAAAAAAATGGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGAc                                                  <  2:782581/145‑1 (MQ=255)                                                                                                      aaCACGGTCAGCGCATTAAGAATCTGCGCAACGTGATGAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCa                                                     <  2:566324/142‑1 (MQ=255)                                                                                                       acagggtgggcgcATTCAGAACCTGGGCAGGGGGAGAAGAAAAACGGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACt                                           <  1:625723/142‑1 (MQ=255)                                                                                                                gcgcATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACt                                  >  1:52036/1‑151 (MQ=255)                                                                                                                 cgcATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTg                                 >  2:652588/1‑151 (MQ=255)                                                                                                                 catagccaacaccggcgcAGAGTGAGGAATAAAACTGAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTcacca                                    <  1:878460/134‑1 (MQ=255)                                                                                                                     ttCAGAATCTGCGAAGCGTGAGGAGAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTcaccac                                   <  1:494092/145‑1 (MQ=255)                                                                                                                       cAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAAt                           >  2:396005/1‑151 (MQ=255)                                                                                                                        aGAATCTCCACAAGGTGATGAAAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGa                                <  1:705637/145‑1 (MQ=255)                                                                                                                        aGAATCCGCGCACCGTAATAACAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATc                          <  1:318442/151‑1 (MQ=255)                                                                                                                          aaTCGGCGCAACGTGAAGAGCAAAATTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATaaa                            <  1:109022/147‑1 (MQ=255)                                                                                                                          aaTCGGCGAAACGTGATGAGAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTg                        <  2:623180/151‑1 (MQ=255)                                                                                                                             ctgcgaagggTGATGAAAAAAGCTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCt                         <  2:143512/138‑1 (MQ=255)                                                                                                                              tGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTAt                    >  1:639169/1‑151 (MQ=255)                                                                                                                              ggcgcAAAGAGAAGAACAAAACTAAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTAt                    <  1:531783/150‑1 (MQ=255)                                                                                                                                cgcAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCg                  >  1:756864/1‑151 (MQ=255)                                                                                                                                  cAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCgg                 >  1:857974/1‑150 (MQ=255)                                                                                                                                            agaaaaaCTGATAACACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGaa       <  1:26497/147‑1 (MQ=255)                                                                                                                                            aaaaaacCTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCaa              <  2:107740/140‑1 (MQ=255)                                                                                                                                                aaaCTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGt  <  1:229165/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                CACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGT  >  NC_000913/1755721‑1756014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑