Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,163	G→A	R158C (CGT→TGT)	sapF ←	antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,163	0	G	A	100.0%	212.3 / NA	56	R158C (CGT→TGT)	sapF	antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

TACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTG  >  NC_000913/1352024‑1352310                                                                                                                                            |                                                                                                                                                    taCGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTc                                                                                                                                          >  1:816207/1‑151 (MQ=255)       aTGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTg                                                                                                                                    <  2:334081/151‑1 (MQ=255)                ttttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACatat                                                                                                                           >  2:239750/1‑151 (MQ=255)                ttttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACata                                                                                                                            <  2:399060/150‑1 (MQ=255)                 tttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACatat                                                                                                                           <  1:27973/150‑1 (MQ=255)                 tttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATg                                                                                                                          >  1:96108/1‑151 (MQ=255)                  ttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGc                                                                                                                         >  2:189184/1‑151 (MQ=255)                       gTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGAt                                                                                                                     >  2:606964/1‑150 (MQ=255)                       gTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGAt                                                                                                                     <  1:401778/150‑1 (MQ=255)                       gTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATa                                                                                                                    <  2:211736/151‑1 (MQ=255)                         aaTTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGt                                                                                                                  >  2:163027/1‑151 (MQ=255)                           ttCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTa                                                                                                                 >  1:415544/1‑150 (MQ=255)                               aGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGa                                                                                                            <  1:796969/151‑1 (MQ=255)                                      gATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATc                                                                                                     >  2:14645/1‑151 (MQ=255)                                          aaTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCgg                                                                                                  <  1:791617/150‑1 (MQ=255)                                          aaTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGt                                                                                                 >  2:751590/1‑151 (MQ=255)                                           aTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTa                                                                                                <  2:907656/151‑1 (MQ=255)                                               aCTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagcag                                                                                            <  2:436172/151‑1 (MQ=255)                                                   cGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGccc                                                                                         >  2:670241/1‑150 (MQ=255)                                                   cGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCa                                                                                        <  2:518499/151‑1 (MQ=255)                                                    gAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCa                                                                                        >  2:525222/1‑150 (MQ=255)                                                     aaCGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCacc                                                                                      >  1:940935/1‑149 (MQ=255)                                                      aCGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAAcc                                                                                     >  2:555351/1‑151 (MQ=255)                                                      aCGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAAcc                                                                                     >  2:719801/1‑151 (MQ=255)                                                        gCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCAt                                                                                   <  2:366376/151‑1 (MQ=255)                                                            cGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGc                                                                               >  2:782038/1‑151 (MQ=255)                                                            cGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGc                                                                               >  2:407025/1‑151 (MQ=255)                                                                            gCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCt                                                               <  2:514270/151‑1 (MQ=255)                                                                               aGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGtt                                                            >  1:436222/1‑151 (MQ=255)                                                                                  gCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGc                                                         >  2:480361/1‑151 (MQ=255)                                                                                     tCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGAc                                                      >  2:277492/1‑151 (MQ=255)                                                                                     tCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGAc                                                      >  1:653106/1‑151 (MQ=255)                                                                                        tCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCt                                                   >  2:897303/1‑151 (MQ=255)                                                                                         cGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCt                                                   <  2:54364/150‑1 (MQ=255)                                                                                          ggCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGt                                                 >  1:918295/1‑151 (MQ=255)                                                                                           gCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGtt                                                <  2:787039/151‑1 (MQ=255)                                                                                           gCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGtt                                                >  1:884187/1‑151 (MQ=255)                                                                                            cAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTc                                               >  2:439398/1‑151 (MQ=255)                                                                                             aataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTcc                                              <  1:588330/151‑1 (MQ=255)                                                                                              ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCg                                             <  2:121504/151‑1 (MQ=255)                                                                                                  tGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTc                                         >  1:724020/1‑151 (MQ=255)                                                                                                    aCTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCa                                       <  1:743980/151‑1 (MQ=255)                                                                                                     cTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTcc                                        >  1:610130/1‑149 (MQ=255)                                                                                                       tttGGGCGCAATATCATCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGGTTGCGACGCTGTTCCGGTTCCAgg                                     >  1:563328/1‑150 (MQ=255)                                                                                                       tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAg                                      <  2:96117/149‑1 (MQ=255)                                                                                                                aaTATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCa                           <  2:22606/151‑1 (MQ=255)                                                                                                                aaTATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCa                           <  2:737593/151‑1 (MQ=255)                                                                                                                 atatCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAg                          <  1:851621/151‑1 (MQ=255)                                                                                                                   atCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAgg                         <  2:928417/150‑1 (MQ=255)                                                                                                                     cAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGgcgc                      <  1:223341/151‑1 (MQ=255)                                                                                                                         gcgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTg                  >  2:911321/1‑151 (MQ=255)                                                                                                                          cgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTgg                 <  2:849383/151‑1 (MQ=255)                                                                                                                          cgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTgg                 <  2:181857/151‑1 (MQ=255)                                                                                                                                ccagacccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATc           >  2:688504/1‑151 (MQ=255)                                                                                                                                      ccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAAt     <  2:33149/151‑1 (MQ=255)                                                                                                                                         gacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAATATCCAGAATTTg  <  1:695642/151‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                    TACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTG  >  NC_000913/1352024‑1352310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑